In this Letter, the femtosecond laser holographic two-photon polymerization (HTPP) method is adopted to rapidly realize a unique lab-in-a-needle (LIN) device by manufacturing microtube arrays inside a needle. The HTPP method is to modulate a Gaussian beam into a ring Bessel beam by a spatial light modulator (SLM) loaded with a Bessel hologram, and can fabricate microtube arrays with controllable inside diameter (1-10μm) and designable patterns on such complex three-dimensional (3D) substrates by optimizing experimental parameters. A single LIN device can be processed by this method in about 4min, which is not possible with traditional micronano technology and is much faster than the traditional two-photon polymerization method (at least several hours). To further demonstrate the functionality of this LIN device, a particle separation experiment is carried out. For the purpose of achieving greater functionality and integration of the on-chip system, this HTPP method provides a powerful processing method for integrating 3D functional microstructures on 3D nonplanar substrates.