Abstract Many-body methods that use Gaussian-wave packets to describe nucleon spatial distribution have been widely employed for depicting various phenomena in nuclear systems, in particular clustering. So far, however, the chiral effective field theory, a state-of-the-art theory of nuclear force, has not been applied to such methods. In this paper, we give the formalism to calculate the two-body matrix elements of the chiral two-nucleon forces using the Gaussian-wave packets. We also visualize the matrix elements and investigate the contributions of the central and tensor forces. This work is a foothold towards an ab initio description of various cluster phenomena in view of nucleons, pions, and many-nucleon forces.