Monitoring the dust cloud dispersion process inside testing equipment like the MIKE3 minimum ignition energy device is useful for assessing the ignitability characteristics of different dust materials. However, it is difficult to comprehensively capture the relevant micro-scale particle properties and macro-scale flow behavior of a dust cloud dispersion using a single measurement method. Thus, the objective of this work is to combine two complementary laser diagnostic techniques to obtain quantitative particle and flow properties from dust clouds in the MIKE3 glass tube. For this experiment, thirty repeated runs for each method were conducted using small (15-mg) aluminum powder samples. No ignition electrodes are included in the test geometry to establish a baseline flow configuration for the glass tube, although measurements are still made at the characteristic time delay and location of ignition tests. The first technique, digital in-line holography (DIH) is used for three-dimensional micro-scale particle diagnostics, yielding particle number (n = 78 ± 20), size (D̅ = 18.9 ± 0.6 µm), and three-component velocity (v̅x = 0.05 ± 0.05 m/s, v̅y = 0.60 ± 0.09 m/s, v̅z = − 0.07 ± 0.16 m/s) measurements. The second laser diagnostic technique, particle image velocimetry (PIV), is used for macro-scale flow diagnostics, yielding two-dimensional flow velocity and vorticity vector field measurements. Particle sizes are observed to follow a lognormal distribution (μ = 2.89, σ = 0.31), and a discrepancy between in-situ and ex-situ sizing results is identified for particle sizes larger than ~55 µm. Two-dimensional particle velocities follow a Weibull distribution (θ = 0.86, β = 2.12), and reasonable agreement is found between the DIH and PIV velocity measurements.