Human resources refer to a special and unique field as they are the most valuable but also the most costly factor of production. The aim of the research is to analyze the level of motivation of university teachers in Slovakia in terms of time and age, and to define the motivational needs of university teachers. The method of sociological questioning is used. The collected data from 2016 university teachers from Slovak technical universities are analyzed using the Tukey HSD test. Based on the research results, it can be stated that university teachers are the most motivated by relational and financial motivational factors. There is a significant change in the level of average importance of motivational factors across time (years), but there is no change in their structure. In terms of the age factor, significant differences over time are identified. Finally, Slovak teachers display the need for a more respected social status and a better image of their profession. The research findings will help university managers in raising the level of teachers’ motivation and in designing motivation programs.