Tubules In Presence Research Articles

Basolateral regulation of pHi in isolated snake renal proximal tubules in presence and absence of bicarbonate.

Intracellular pH (pHi) and its basolateral regulation were studied in isolated proximal-proximal and distal-proximal segments of garter snake (Thamnophis spp.) renal tubules with oil-filled lumens in HEPES-buffered and in HEPES-HCO-3-buffered media (pH 7.4 at 25 degrees C). pHi was measured with the pH-sensitive fluorescent dye 2',7'-bis(2-carboxyethyl)-5,6-carboxyfluorescein (BCECF) under resting conditions and in response to NH4Cl pulse. Resting pHi (approximately 7.1-7.2) and its response to and rate of recovery (dpHi/dt) from an NH4Cl pulse were not affected by the presence or absence of HCO-3 in either segment. Rate of recovery was depressed by Na+ removal in distal-proximal segments only and only in HEPES buffer. It was not affected by removal of Cl- or of both Na+ and Cl- or by reduction in membrane potential through addition of Ba2+ (5 mM) or high K+ (75 mM) in either segment in either HEPES or HEPES-HCO-3 buffer. The Na+/H+ exchange inhibitor ethylisopropylamiloride (EIPA) (100 microM) and the anion exchange inhibitor DIDS (100 microM) reduced dpHi/dt in the distal-proximal segments only and only in HEPES-HCO-3 buffer. The H+-ATPase inhibitor bafilomycin (1 microM), H+-K+-ATPase and K+/NH+4 exchange inhibitor Schering 28080 (10-100 microM), organic cation efflux inhibitor tetrapentylammonium (25 microM-20 mM), and K+ channel blocker tetraethylammonium (20 mM) had no effect on dpHi/dt in either segment. These data do not clearly support basolateral regulation of pHi in snake proximal renal tubules by commonly recognized Na+-dependent or Na+-independent acid or base transporters.

The effect of barbiturates on microtubular assembly

Purified tubulin was prepared from fresh pig brain. Purity was determined by gel electrophoresis. Polymerization and depolymerization of microtubules were studied as a function of the presence (10 −3 M ) and absence of phenobarbital. The drug markedly depressed tubular assembly. Dialysis of presumably protein bound phenobarbital affected recovery of polymerization properties favorably, but only to 65% of control levels. Protein assembly was determined by biophysical methodology as incubation of tubulin proceeded at 37°C. Phenobarbital added to assembly medium interrupted polymerization and reduced both viscosity and turbidity. The various preparations were monitored by transmission electron microscopy. The lessened production of tubules in presence of drug was characterized by the presence of tubules of only 10 μ m in length. The significance of this drug effect on CNS development is discussed.

PAH transport by snake proximal renal tubules: differences from urate transport.

ARTICLESPAH transport by snake proximal renal tubules: differences from urate transportWH DantzlerWH DantzlerPublished Online:01 Mar 1974https://doi.org/10.1152/ajplegacy.1974.226.3.634MoreSectionsPDF (2 MB)Download PDF ToolsExport citationAdd to favoritesGet permissionsTrack citations ShareShare onFacebookTwitterLinkedInWeChat Previous Back to Top Next Download PDF FiguresReferencesRelatedInformation Cited ByRenal Morphology6 July 2016Transport of Organic Substances by Renal Tubules6 July 2016Osmoregulation and Excretion19 March 2014Osmotic and Ionic Regulation in Reptiles5 February 2014Challenges and intriguing problems in comparative renal physiologyJournal of Experimental Biology, Vol. 208, No. 4Action of EGF and PGE2 on basolateral organic anion uptake in rabbit proximal renal tubules and hOAT1 expressed in human kidney epithelial cellsC. Sauvant, D. Hesse, H. Holzinger, K. K. Evans, W. H. Dantzler, and M. Gekle1 April 2004 | American Journal of Physiology-Renal Physiology, Vol. 286, No. 4Regulation of renal proximal and distal tubule transport: sodium, chloride and organic anionsComparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, Vol. 136, No. 3Renal organic anion transport: a comparative and cellular perspectiveBiochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1566, No. 1-2Method for measuring luminal efflux of fluorescent organic compounds in isolated, perfused renal tubulesApichai Shuprisha, Stephen H. Wright, and William H. Dantzler1 November 2000 | American Journal of Physiology-Renal Physiology, Vol. 279, No. 5PKC regulation of organic anion secretion in perfused S2 segments of rabbit proximal tubulesApichai Shuprisha, Ronald M. Lynch, Stephen H. Wright, and William H. Dantzler1 January 2000 | American Journal of Physiology-Renal Physiology, Vol. 278, No. 1Real-time assessment of α-ketoglutarate effect on organic anion secretion in perfused rabbit proximal tubulesApichai Shuprisha, Ronald M. Lynch, Stephen H. Wright, and William H. Dantzler1 October 1999 | American Journal of Physiology-Renal Physiology, Vol. 277, No. 4Amino acid fluxes in rat thin limb segments of Henle’s loop during in vitro microperfusionOlga H. Brokl, and William H. Dantzler1 August 1999 | American Journal of Physiology-Renal Physiology, Vol. 277, No. 2Basolateral regulation of pHiin isolated snake renal proximal tubules in presence and absence of bicarbonateWilliam H. Dantzler, Oscar K. Serrano, Diane E. Abbott, Yung Kyu Kim, and Olga H. Brokl1 June 1999 | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, Vol. 276, No. 6Transport of organic molecules by reptilian nephronsJournal of Experimental Zoology, Vol. 283, No. 7Vertebrate Renal System1 January 2011Renal tubular secretion of organic anionsAdvanced Drug Delivery Reviews, Vol. 25, No. 2-3Comparative aspects of renal urate transportKidney International, Vol. 49, No. 6Renal Transport of Organic Acids and Bases in Nonmammalian VertebratesChapter 15 XenobioticsRenal vasculature and excretory system of the agamid lizard,Ctenophorus ornatusJournal of Morphology, Vol. 217, No. 3Evaluation of Function in Single Segments of Isolated Renal Blood Vessels, Nephrons, and Collecting Ducts1 January 2011Comparative Physiology of the Kidney1 January 2011Renal Excretion and Tubular Transport of Organic Anions and Cations1 January 2011Tetraethylammonium transport by snake renal brush-border membrane vesiclesPfl�gers Archiv European Journal of Physiology, Vol. 418, No. 4[11] Comparative kidney tubule sources, isolation, perfusion, and functionOrganic acid (or anion) and organic base (or cation) transport by renal tubules of nonmammalian vertebratesJournal of Experimental Zoology, Vol. 249, No. 3Effects of ureteral occlusion and ethacrynic acid infusion on renal prostaglandin degradation in the dogActa Physiologica Scandinavica, Vol. 133, No. 4Luminal and peritubular steps in renal transport ofBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, Vol. 906, No. 2Effects of SITS on urate transport by isolated, perfused snake renal tubulesPfl�gers Archiv European Journal of Physiology, Vol. 403, No. 1The renal handling of terephthalic acidToxicology and Applied Pharmacology, Vol. 77, No. 1Lack of effect of low [Ca2+], La3+, and pyrazinoate on urate transport by isolated, perfused snake renal tubulesPflügers Archiv European Journal of Physiology, Vol. 401, No. 3Inhibitory Effect of 6-Aminonicotinamide on the Renal Transport of Para-Aminohippurate in the RatJapanese Journal of Pharmacology, Vol. 36, No. 4Studies of organic anion and cation transport in isolated segments of proximal tubulesKidney International, Vol. 22, No. 5Studies on nonmammalian nephronsKidney International, Vol. 22, No. 5Comparative Physiology of the Renal Transport of Organic SolutesIsolated nephron segments as pharmacological toolsTrends in Pharmacological Sciences, Vol. 2Renal excretion of urate: Factors determining the actions of drugsKidney International, Vol. 18, No. 5Perfusion of Isolated Mammalian Renal TubulesMicroperfusion of Isolated TubulesUrate Excretion in Nonmammalian VertebratesRenal Functions in Hens Fed Graded Dietary Levels of Ochratoxin A13 March 2009 | Acta Pharmacologica et Toxicologica, Vol. 38, No. 3Renal tubular mechanisms of organic solute transportKidney International, Vol. 9, No. 2The Renal Excretion of DrugsThe Use of the Isolated Tubule Preparation for the Investigation of DiureticsRenal ATPase as a Receptor for Drugs Acting on the KidneyComparative pharmacology of uricosuric drugsGeneral Pharmacology: The Vascular System, Vol. 7, No. 1 More from this issue > Volume 226Issue 3March 1974Pages 634-641 Copyright & PermissionsCopyright © 1974 by American Physiological Societyhttps://doi.org/10.1152/ajplegacy.1974.226.3.634PubMed4817417History Published online 1 March 1974 Published in print 1 March 1974 Metrics

Effect of metabolic alkalosis and acidosis on tubular urate secretion in water snakes.

ARTICLESEffect of metabolic alkalosis and acidosis on tubular urate secretion in water snakesWH DantzlerWH DantzlerPublished Online:01 Sep 1968https://doi.org/10.1152/ajplegacy.1968.215.3.747MoreSectionsPDF (1 MB)Download PDF ToolsExport citationAdd to favoritesGet permissionsTrack citations ShareShare onFacebookTwitterLinkedInWeChat Previous Back to Top Download PDF FiguresReferencesRelatedInformation Cited ByInitial Process in Urine Formation6 July 2016Transport of Inorganic Ions by Renal Tubules6 July 2016Transport of Organic Substances by Renal Tubules6 July 2016Renal plasticity in response to feeding in the Burmese python, Python molurus bivittatusComparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, Vol. 188Osmoregulation and Excretion19 March 2014Osmotic and Ionic Regulation in Reptiles5 February 2014Ammonia functions as a regulatory molecule to mediate adjustments in glomerular filtration rate in response to changes in metabolic rateMedical Hypotheses, Vol. 57, No. 6Basolateral regulation of pHiin isolated snake renal proximal tubules in presence and absence of bicarbonateWilliam H. Dantzler, Oscar K. Serrano, Diane E. Abbott, Yung Kyu Kim, and Olga H. Brokl1 June 1999 | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, Vol. 276, No. 6Vertebrate Renal System1 January 2011Renal Transport of Organic Acids and Bases in Nonmammalian VertebratesComparative Physiology of the Kidney1 January 2011Net tubular secretion of bicarbonate by the alligator kidney. Antimammalian response to acetazolamideKidney International, Vol. 28, No. 5Effects of temperature on acid and base excretion in a lizard,Dipsosaurus dorsalisJournal of Comparative Physiology B, Vol. 154, No. 1Acid and base excretion: Assessment and relationships to diet and urine compositionComparative Biochemistry and Physiology Part A: Physiology, Vol. 66, No. 3Kidney and bladder function in a uricotelic treefrog (Phyllomedusa sauvagei)Journal of Comparative Physiology ? B, Vol. 133, No. 3Urate Excretion in Nonmammalian VertebratesDrugs Affecting the Renal Handling of Uric AcidThe excretion of hyperosmotic urine and other aspects of the electrolyte balance of the lizard Amphibolurus maculosusComparative Biochemistry and Physiology Part A: Physiology, Vol. 54, No. 3Renal function in goutThe American Journal of Medicine, Vol. 59, No. 5Urate and cation interactions in the liquid and precipitated fractions of avian urine, and speculations on their physico-chemical stateComparative Biochemistry and Physiology Part A: Physiology, Vol. 48, No. 1Renal mechanisms for regulation of uric acid excretion, with special reference to normal and gouty manSeminars in Arthritis and Rheumatism, Vol. 2, No. 1Excretion of urate salts by reptilesComparative Biochemistry and Physiology Part A: Physiology, Vol. 41, No. 3Relation of potassium to urate accumulation by kidney slices from desert spiny lizards (Sceloporus magister)Comparative Biochemistry and Physiology Part A: Physiology, Vol. 40, No. 2Plasma-Renin-Aktivität der Ratte bei systemischer Alkalose und AcidoseZeitschrift für die gesamte experimentelle Medizin einschließlich experimentelle Chirurgie, Vol. 153, No. 3Water and electrolyte balance of the desert iguana, dipsosaurus dorsalis, in its natural habitatComparative Biochemistry and Physiology, Vol. 35, No. 4Absorption of water and electrolytes from the cloaca of an Australian lizard, Varanus gouldii (gray)Comparative Biochemistry and Physiology, Vol. 35, No. 3Comparison of renal tubular transport of urate and PAH in water snakes: Evidence for differences in mechanisms and sites of transportComparative Biochemistry and Physiology, Vol. 34, No. 3Comparison of renal function of bull and water snakes (Pituophis melanoleucus and natrix sipedon)Comparative Biochemistry and Physiology, Vol. 32, No. 2 More from this issue > Volume 215Issue 3September 1968Pages 747-751 Copyright & PermissionsCopyright © 1968 by American Physiological Societyhttps://doi.org/10.1152/ajplegacy.1968.215.3.747PubMed5671016History Published online 1 September 1968 Published in print 1 September 1968 Metrics