Tuberous Roots Research Articles

Pharmacological Activities of the Roots of Stemona tuberosa Medicinal Plant: An Overview

: Since childhood, plants and plant products have been used extensively as medicine. The majority of rural residents still rely on natural resources and the old-fashioned medical system for their daily needs. The biological richness of Arunachal Pradesh is renowned, as is its rich cultural diversity. The native population uses a number of aromatic and medicinal plant species to cure a wide range of illnesses. The current review deals with the biological activity of Stemona tuberosa, a possible medicinal plant species of Arunachal Pradesh. Extract from tuberous roots has numerous properties, including anti-bacterial, anti-inflammatory, anti-tobacco mosaic virus, antitussive, wound healing, and anti-herpes. The roots' powder is employed as a preservation and insect deterrent. Alkaloids, sugars and glycosides, phenolic compounds, trace amounts of proteins, saponin, flavonoids, and gum mucilage, were all found during the phytochemical analysis, which shows that the species has a significant potential for therapeutic use. As a result, it is possible to think of the examined species as a potential source of beneficial medications.

Comparative Efficacy of Ocimum basilicum and Ocimum gratissimum Essential Oils Against Cyclas puncticollis Infesting Sweet Potatoes in Burkina Faso

Aims: The aim of the present study was to compare the insecticidal effect of the essential oils Ocimum basilicum and Ocimum gratisiimum on the sweet potato weevil Cyclas punticollis. Study Design: The aim of this approach was to contribute to the management of the sweet potato weevil in Burkina Faso. Place and Duration of Study: The experiments were carried out at the INERA Entomology laboratory in Bobo-Dioulasso during the cropping season 2022-2023. Methodology: Five essential oil concentrations (25; 50; 75; 100 and 125 μL/L) were used. Toxicity tests were carried out by fumigation in jars by depositing a charge of essential oil on a 6 cm disc of filter paper. Batches of ten C. puncticollis adults were introduced into the jars containing a fresh, healthy tuberous root of the sweet potato and immediately closed. Each test was repeated five times. The number of dead insects in each batch (treated and untreated jars) was counted every 30 min up to 5 h of exposure and for 4 days (24 h, 48 h ,72 h and 96h) per observation. Results: The results showed that the different oils had an insecticidal effect on C. puncticollis and that these oils contained chemical substances responsible for the mortality observed. Indeed, mortality rates of 100% were observed after 2 hours of exposure of the weevils with the 125µL/L concentration of O. gratissimum, followed by concentrations of 100µL/L, 75µL/L, 50µL/L after 24 hours and 48 hours with 25µL/L. With O. basilicum essential oil, 100% mortality was observed after 24 hours of weevil exposure with O. basilicum concentrations of 125µL/L and 100µL/L, followed by 98% mortality with concentrations of 75µL/L, 50µL/L and 25µL/L after 96 hours. Conclusion: These results suggest that the essential oils of Ocimun gratissimun and Ocimun basilicum can be used in the management of C. puncticollis. However, it would be necessary to carry out a concrete application of these oil extracts on large potato stocks in order to confirm or not their efficacy.

Effects of Bio and water-soluble fertilizers on sweet potato yield, quality and soil properties in a continuous cropping system under plastic film-mulched drip-fertigated field conditions.

To verify an effective approach for alleviating problems associated with the consecutive monoculture of sweet potato, five fertilizer treatments were designed under plastic film-mulched, drip-fertigated conditions in fields continuously planted with sweet potato over five years. These treatments included: (1) no fertilizer application, (2) basic application of water-soluble (WS) fertilizer, (3) basic application of biofertilizer (BF) and WS fertilizer, (4) split application of WS fertilizer, and (5) combined basic application of BF and split application of WS fertilizer. The effects of BF and WS fertilizer applications on yield, quality, and soil properties were evaluated. Fertilizer applications had positive effects on increasing soil activities and nutrients compared with no fertilizer application. Split fertigation with WS fertilizer increased the activities of soil dehydrogenase (DHA) by 10.94 ~ 14.74%, alkaline phosphatase (ALP) by 4.97 ~ 5.13%, and soil organic matter (SOM) by 10.43 ~ 12.47% in the second year compared with a single application. In both years, split fertigation exerted positive effects on increasing dry matter (DM) accumulation in tuberous roots and increased the productive efficiency in nitrogen (N), phosphorus (P), and potassium (K) fertilizers. BF application for two consecutive years increased the activities of soil sucrase by 7.05 ~ 17.83%, DHA by 18.65 ~ 21.34%, ALP by 6.87 ~ 7.03%, soil available P by 18.34 ~ 28.10%, and SOM by 8.18 ~ 10.17% compared with the no BF application. BF also increased the root yield by 8.88 ~ 14.14%, the carotenoid content in tuberous roots by 20.38 ~ 30.64%, and the K utilization efficiency by 11.09 ~ 14.97%. The combination of BF and split fertigation for two consecutive years was most conducive to the activation of soil nutrients, maintenance of soil fertility, and improvement in yield and quality, which could mitigate problems associated with the consecutive monoculture of sweet potato.

Association between dietary diversity and lipid indices among Indian Kho-Kho players

Purpose Dietary diversity (DD) is a proxy indicator of nutrient adequacy and has been propagated as a health beneficial component of dietary behavior. This study aims to understand DD and its association with lipid indices among Indian Kho-Kho players. Design/methodology/approach In all, 50 Indian Kho-Kho players aged between 16 years and 31 years belonging to different regions of India were recruited. Dietary diversity score (DDS; food groups/day) and food variety score (FVS; number of food items/day) were considered to assess the DD. The United Nations Food and Agricultural Organization food frequency questionnaire was used as a tool. Serum total cholesterol (TC), triglycerides (TG), LDL, VLDL and HDL were measured. Findings The DDS and FVS showed that athletes were consuming approximately 7 of 9 food groups (Mean ± SD: 6.5 ± 1.3) and 27 varieties of foods (Mean ± SD:27.0 ± 8.6) from 9 food groups per day. This study could not find significant differences for DDS and FVS among athletes belonging to different regions. FVSs of organ meat, eggs and spices and condiments were significantly positively associated with TG (p < 0.036, p < 0.020 and p < 0.001) and VLDL (p < 0.054, p < 0.021 and p < 0.001). LDL was inversely significantly associated with FVS of cereals, white root and tubers (p < 0.032). Research limitations/implications DDS and FVS were not associated with lipid indices among Kho-Kho players. However, individual FVS of organ meat, eggs and spices and condiments may increase TG and VLDL. Optimum consumption of cereals and root tubers may help to maintain LDL levels among Kho-Kho players. Originality/value It was found that daily consumption of organ meat and eggs may increase TG and VLDL, and optimum consumption of cereals and white roots and tubers may help to maintain LDL. High energy and fat foods may act as mediators along with spices and condiments in increasing TG and VLDL.

Genome-Wide Identification of the bHLH Gene Family in Callerya speciosa Reveals Its Potential Role in the Regulation of Isoflavonoid Biosynthesis

Callerya speciosa (Champ. ex Benth.) Schot is a significant leguminous plant valued for its edible tuberous roots, which are a plentiful source of isoflavonoids. Basic helix–loop–helix (bHLH) transcription factors (TFs) have been reported to regulate secondary metabolism in plants, especially flavonoid biosynthesis. However, the bHLH genes in C. speciosa have not yet been reported, and their regulatory role in isoflavonoid biosynthesis remains unexplored. Here, 146 CsbHLH genes were identified in the C. speciosa genome, classifying them into 23 subfamilies based on the gene structures and phylogenetic relationships. All the CsbHLH proteins contained both motifs 1 and 2, whereas motif 8 was only distributed in subgroup III (d + e). Collinearity analysis demonstrated that fragmental replications are the primary driver of CsbHLH evolution, with the majority of duplicated CsbHLH gene pairs experiencing selective pressure. Nine candidate CsbHLH genes were found to play a potential role in regulating isoflavonoid biosynthesis through a combination of gene-to-metabolite correlation analysis and weighted gene co-expression network analysis (WGCNA). Additionally, the cis-regulatory elements and response to MeJA of these nine genes were characterized and confirmed through quantitative real-time PCR (qRT-PCR) analysis. Among them, three CsbHLHs (CsbHLH9, CsbHLH89, and CsbHLH95) were selected for further investigation. Yeast two-hybrid (Y2H), dual-luciferase (LUC) assays, bimolecular fluorescence complementation (BiFC) assays, and transient transformation demonstrated that CsbHLH9 acted as a transcriptional activator through its interaction with CsMYB36 and binding to the promoters of isoflavonoid biosynthesis genes in a MeJA-induced manner, such as CsIFR2, CsI3′H2, and CsCHS4, to promote isoflavonoid (calycosin, calycosin-7-o-glucoside, and formononetin) accumulation. Our results establish a basis for the functional analysis of bHLH genes and investigations into the molecular mechanisms underlying isoflavonoid biosynthesis in C. speciosa.

Origin and ontogeny of the xylopodium in Cerrado legumes: Role of the hypocotyl and ecological significance

Bud-bearing underground organs play a role on plant persistence in different fire-prone ecosystems worldwide, but their origin, development and anatomical features are still a matter of inquiry. The xylopodium, for instance, seem to originate from the hypocotyl or primary root, or both, usually associated with tuberous roots. Because this structure is commonly found in legumes across the Brazilian savannas (Cerrado), understanding its formation is important for unveiling its relevance for post-fire resprouting. Here we aimed to investigate the role of the hypocotyl on the development of six Cerrado legume shrubs, as well as histochemical features and ecological implications from seedlings to adult plants in this frequently-burned ecosystem. Seeds of the six study species were collected in central Brazil, set to germinate and cultivated until the stages of four, six, nine, and twelve months. At each stage, the region correspondent to the hypocotyl was delimited and anatomical and histochemical analyses performed. Over time, the hypocotyl region underwent a tuberization process, during which the cortex was progressively replaced by the formation of secondary xylem through intense cambial activity. Before the twelve-month-old stage, all species presented buds, mainly cotyledonary, and reacted to starch, phenols and pectins. Early hypocotyl tuberization drives the formation of the xylopodium from seedlings to adulthood, and the presence of buds and the accumulation of reserves during its formation is a fundamental process driving species’ persistence after fire.

Design and experiment of a deviation information detection mechanism for sugar beet harvesters based on agricultural machinery and agronomy integration

To address large geometric shape differences, poor ridge distribution straightness and the uncertain spatial distribution of beet roots during the harvesting period, as well as damage caused by the inaccurate row correction detection mechanisms of combine harvesters, this study conducts a design analysis and performance tests of deviation information detection mechanisms based on the integration of agricultural machinery and agronomy. The agronomic process of mechanized sugar beet production was analyzed, the geometric dimensions of root tubers under typical planting patterns of typical varieties in main sugar beet production areas were measured, and a three-dimensional geometric model of beet root tubers was established. A device for measuring ridge shape and root tuber distribution was designed, and agronomic parameters during the harvesting period, such as plant spacing, ridge height, unearthed height, and deviation distance of beet root tubers, were measured and analyzed. A spatial model of ridge shape and beet root tuber growth distribution on the ridge during harvesting was established. The overall structure of the deviation information detection mechanism was analyzed and designed, and the structural forms and key parameters of key mechanisms, such as left‒right swing detection and up‒down floating profiling, were designed on the basis of agronomic parameter analysis. Using the missed detection rate as the evaluation index, field performance tests of the deviation information detection mechanism were conducted. The results revealed that when the average forward speed of the harvester was 0.84 m/s, the average missed detection rate of the deviation information detection mechanism was 0.56%. This method has high adaptability and detection accuracy and achieves a high level of integration for agricultural machinery (detection spatial region of the detection mechanism) and agronomy (beet root distribution spatial region). This study provides a technical basis and methodological reference for improving the quality and efficiency of automatic row correction combined with harvesting operations for crops such as sugar beets.

Effect of blanching and microwave-hot air drying of Asparagus racemosus Willd. tuberous roots on shelf life

The quality of dried tuberous roots of Asparagus racemosus Willd. depends on the drying method and storage conditions. This research aims to compare three methods for drying blanched and non-blanched root slices: tray drying at 60 °C (TD 60C), microwave-assisted hot air drying at 1000W with 60 °C (MW1000+60C), and at 1500W with 60 °C (MW1500+60C). Drying efficiency was evaluated over a one-year shelf life. The blanching was carried out at 80 °C for 5min. MW1000 60C was the most suitable for brightness, total phenolic content (TPC), total flavonoid content (TFC), total saponin content (TSC), and sugar content. Its brightness (L⁎), TPC, TFC, and TSC were 90.90 ± 1.08, 1.96 ± 0.08mg GAE/g DW, 0.69 ± 0.07mg ECE/g DW, and 1256.8 ± 25.1mg SE/g DW, respectively. Total sugar content and total reducing sugar content were 1577.1 ± 17.1mg Glu/g DW and 47.43 ± 2.06mg Glu/g DW, respectively. Blanching before MW1000+60C decreased TPC, TFC, total sugar content, and total reducing sugar content by 31%, 25%, 20%, and 43%, respectively, but did not reduce the TSC. The optimum shelf life was 6 months. The storage for 12 months caused negative changes in moisture content, colour, TPC, TFC, sugar content, and microbial load. The saponin compounds were asparacoside, shatavarin IX, shatavarin V, asparanin A, aspafilioside B, and sarsasapogenin 3-[4''-glucosyl-6''-arabinosylglucoside]. Theasaponin E3 was found in blanched and dried roots after 12 months. Consequently, this research provided guidelines for drying methods and monitoring shelf life.

A New Starches Extracted from Non- Conventional Sources are the Vogue for Pharmaceutical Industry: A Review

Industrially, starch is obtained from cereal grains such as wheat and maize (corn), or from tubers, such as potatoes, tapioca, and arrowroot, or from the pith of the sago plant. By far, the larger part of the starch is obtained from maize, wheat, potato, and tapioca. Starch is present in almost all the tissue such as leaves, roots, tubers, seeds, stems, flowers, etc. of green plants however there are some plants which are grown commercially for the starch which includes cereal such as wheat, corn, sorghum, and rice, tuber mainly potato, root like tapioca and arrowroot, stem of sago, and legume crops mainly pea. Worldwide production of an overview on applications of starch 142 starch is depending on the use of cereals as the raw materials. Normally starch from Conventional sources of starch include sources like cereal corps and legume seeds, tuber crops, and some root tubers is used in large amount in the field of pharmacy. Due to unbelievable increasing demand for starch some nonconventional starch resources have been investigated in recent Years.

Effects of UV-A/B/C on flavonoids and related synthetic enzymes in Tetrastigma hemsleyanum.

Tetrastigma hemsleyanum is a folk and rare medicinal plant, and specifically, it is distributed in the south, China. To investigate the cumulative properties of its medicinal components, we examined the effect of UV light on flavonoid content and related enzyme activity changes in T. hemsleyanum. The leaves and tubers were treated with UV-A, UV-B and UV-C for 1 h, 1L/23D h, 3 h and 3L/21D h (D represents darkness treatment). High-performance liquid chromatography (HPLC) analysis showed that the content of many flavonoids decreased significantly during UV-A treatment, increased after UV-B and UV-C irradiation and accumulated again after darkness treatment. In the root tubers of the UV-A group, naringin content in the 3L/21D h group (0.069 μg/g) was 16.30 times higher than that of 3 h group (0.0042 μg/g). The rutin content was elevated after UV irradiation but was not detected in the CK group. The test results of the enzyme-linked kit indicated that the activities of many enzymes were higher in the UV-A and UV-B irradiation groups than those in the CK group, but the results were reversed in the UV-C treatment. After darkness treatment, the activities of most enzymes were higher than those with UV irradiation alone; F3'5'H activity in the 3L/21D h group (97.25 U/L) was 1.24 times higher than that in the 3 h group (78.12 U/L) in the UV-A-treated group. The study results suggest that appropriate UV-B and UV-C irradiation, as well as darkness supplementation, had a promotive effect on flavonoids in the leaves and root tubers of T. hemsleyanum. Additionally, UV irradiation and darkness treatment enhanced the activity of most enzymes.

Ireland Bros.’ Pan-American Electric Carnival: Canadians’ border-crossing circuits of early cinema

ABSTRACT Early cinema exhibitors George H. and Egerton L. Ireland were better known in Jamaica and Trinidad than in their homeland of Canada. Named the Pan-American Electric Carnival, after a failed bid to show moving pictures at the 1901 Pan-American Exposition, between 1900 and 1908 they toured Canada, the United States, across the Caribbean and into South America. Almost absent from Canadian film history, los hermanos Ireland are mentioned in at least eight national and local film histories from across Latin America, and even in a US history. Yet, few of these recognize connections to each other, let alone the brothers’ roots in Canada. I take the Ireland brothers’ peripatetic travels throughout the Western hemisphere as a curious case that is uniquely Canadian. Although Ireland Bros.’ motivations are unknowable, their inter-colonial journeys can only partly be explained by wanderlust, seeking escape from rural Canadian roots. I propose their significance is the provision of modern entertainment that was not simply a conduit of British colonialism nor merely a channel for American imperialism. Instead, I conclude speculatively that the brothers’ circuits of cinema along Pan-American trade routes reflect an ethos of Pan-Americanism emergent at the turn of the twentieth century.

Belowground systems in tropical savanna: Fabaceae morphoanatomical traits and their relation to fire.

Post-fire regeneration characterizes woody vegetation of the Cerrado. Several species (e.g., from the Fabaceae) can resprout after fire due to the presence of storage bud-bearing belowground structures, such as xylopodia, having the capacity to rapidly allocate resources for the formation of new aboveground shoots, an advantage in fire-prone ecosystems. Therefore, we evaluated the morphoanatomical structure of the belowground organs, buds and their storage to elucidate fire-related functional traits in relation to regeneration. Besides the strong capacity of plants with xylopodia to resprout and/or their associated root suckers to propagate laterally, they also provide protection against pathogens, through the presence of defence compounds. We evaluated the morphoanatomy and performed histochemical tests with the belowground organs of eight legume species collected in open savannas in Central Brazil. Two species presented a taproot tuber and the six remaining species had a xylopodium as belowground organ. All xylopodia had buds on their upper portion. These organs were basically composed of lignified tissue, containing defence (phenolic compounds and lipidic substances), and storage (starch) substances. All xylopodia were associated to tuberous roots, and in two species these roots were also root suckers. Thus, the presence of belowground storage organs, in combination with stored defence compounds, likely facilitates the persistence of the investigated legumes in fire-prone ecosystems.

Selectivity of pre-emergent herbicides in cassava (Manihot esculenta Crantz) and its relationship with the stem cutting planting system

Cassava (Manihot esculenta Crantz, Euphorbiaceae), an important source of food for millions of people in the world, is distributed mainly in Asia, Africa, Latin America, and the Caribbean. Weeds are important biotic constraints on cassava production, and herbicide application can provide effective weed control in this crop. However, the different possibilities of herbicide selectivity (factors associated and not associated with the plants) must be studied before implementation. The aim of this study was to evaluate the effects of the combination of different pre-emergence herbicides and two stem cutting planting systems (vertical and horizontal) on phytotoxicity and several agronomic traits of the cassava crop. The experiment was conducted through a factorial arrangement in a completely random design in Corrientes, Argentina. The factors studied were planting systems (horizontal and vertical position), weed control methods (hoeing or application of diuron, S-metolachlor, pendimethalin or clomazone, 625; 1920; 900 and 1000 g a.i. ha−1, respectively), and cropping seasons (2018–2019 and 2019–2020). The following variables were measured: phytotoxicity, plant height, relative plant stand, total weight of tuberous roots per plant, starch concentration of tuberous roots, and starch production per ha. The highest precipitations occurred in the 2018–2019 cropping season. The vertical planting system in combination with herbicides showed lower phytotoxicity values and higher values of plant height, starch concentration, and starch production per ha than the horizontal planting system. The highest phytotoxicity values were recorded with the application of diuron combined with the horizontal planting system and in the 2018–2019 season, with a 33.33% decrease in relative plant stand compared to the use of hoeing. Starch concentration remained unchanged with the use of clomazone and pendimethalin, whereas the effect of these herbicides on starch production varied with the cropping season, decreasing only in 2019–2020. Starch production was significantly lower with the use of S-metolachlor (up to 55.29% in 2018–2019) and of diuron (up to 60.57% in 2019–2020) than with the use of hoeing. In conclusion, phytotoxicity values were higher for the horizontal planting system than for the vertical system. The herbicide with the highest phytotoxic effect on the cassava crop was diuron; the effect increased when combined with the horizontal planting system in the cropping season with higher rainfall. This work provides the basis for integrated weed management concerning the use of herbicides and their selectivity according to planting systems for cassava crops.

Integrated Transcriptional and Metabolomic Analysis of Factors Influencing Root Tuber Enlargement during Early Sweet Potato Development.

Sweet potato (Ipomoea batatas (L.) Lam.) is widely cultivated as an important food crop. However, the molecular regulatory mechanisms affecting root tuber development are not well understood. The aim of this study was to systematically reveal the regulatory network of sweet potato root enlargement through transcriptomic and metabolomic analysis in different early stages of sweet potato root development, combined with phenotypic and anatomical observations. Using RNA-seq, we found that the differential genes of the S1 vs. S2, S3 vs. S4, and S4 vs. S5 comparison groups were enriched in the phenylpropane biosynthesis pathway during five developmental stages and identified 67 differentially expressed transcription factors, including AP2, NAC, bHLH, MYB, and C2H2 families. Based on the metabolome, K-means cluster analysis showed that lipids, organic acids, organic oxides, and other substances accumulated differentially in different growth stages. Transcriptome, metabolome, and prophetypic data indicate that the S3-S4 stage is the key stage of root development of sweet potato. Weighted gene co-expression network analysis (WGCNA) showed that transcriptome differential genes were mainly enriched in fructose and mannose metabolism, pentose phosphate, selenium compound metabolism, glycolysis/gluconogenesis, carbon metabolism, and other pathways. The metabolites of different metabolites are mainly concentrated in amino sugar and nucleotide sugar metabolism, flavonoid biosynthesis, alkaloid biosynthesis, pantothenic acid, and coenzyme A biosynthesis. Based on WGCNA analysis of gene-metabolite correlation, 44 differential genes and 31 differential metabolites with high correlation were identified. This study revealed key gene and metabolite changes in early development of sweet potato root tuber and pointed out potential regulatory networks, providing new insights into sweet potato root tuber development and valuable reference for future genetic improvement.

Identification and functional analysis of the Dof transcription factor genes in sugar beet.

In this study, members of the BvDof transcription factor family were identified in the beet genome data (Beta vulgaris L.) Through systematic analysis, 22 BvDof family genes were found in the beet genome, and they were divided into nine groups by phylogenetic analysis. Fifteen members of the BvERF family were involved in the transition to rapid root tuber growth. There was a tandem replication during the generation of the Dof gene family in sugar beet. Bv1_zfms, Bv_ofna, Bv5_racn, and Bv6_augo may be involved in the regulation of secondary cambium development in the beet root tuber. Bv9_nood, Bv1_zfms, and Bv6_cdca may be related to the growth rate of root tubers. The results provide a reference for further elucidating the molecular mechanism of the BvDof transcription factor, which regulates the development of beet root tubers.

Recognition of Pleuropterus angulatus (Polygonaceae) as a separate species: evidence from morphological and molecular data

On the basis of morphological and molecular data, we demonstrate that Polygonum multiflorum var. angulatum actually belongs to the genus Pleuropterus. As a consequence, a nomenclatural change (Pleuropterus angulatus comb. et stat. nov.) is proposed. This species is closely related to Pleuropterus multiflorus, but differ from the latter in root tuber color (white vs. yellow-brown in P. multiflorus), vascular strands (absent vs. present), branchlets (densely papillate vs. smooth), and vein (densely papillae vs. non-papillate), and extra-floral nectaries (deeper, thicker around the lips vs. shallow, thin).

Alkaloids from Stephania cephalantha and their enzyme inhibitory activities

A new protoberberine type alkaloid, 11-diethylaminomethyl corydalmine (1), and 27 known alkaloids (2-28) were isolated from the tuberous roots of Stephania cephalantha. Their structures were identified by spectral analysis. Enzyme inhibitory activities of the compounds on acetylcholinesterase (AChE), tyrosinase and α-glucosidase were evaluated. Compounds 13, 16 and 18 exhibited comparable AChE inhibitory activities to the positive control huperzine A, with the IC50 values of 0.046, 0.048, and 0.057 mg/mL, respectively.

Antifungal activity of 2-chloro-5-trifluoromethoxybenzeneboronic acid and inhibitory mechanisms on Geotrichum candidum from sour rot Xiaozhou mustard root tuber

Xiaozhou mustard (Brassica napiformis) root tuber, a traditional fermented vegetable, has a long history in Rongan County, Guangxi Province. However, the frequent occurrence of root tuber sour rot by Geotrichum candidum (G. candidum) has seriously reduced Xiaozhou mustard production and quality in recent years. The objective of the present study is to investigate the antifungal efficacy of 2-chloro-5-trifluoromethoxybenzeneboronic acid (Cl-F-BBA) against G. candidum and its possible mechanisms. The results revealed that a concentration of 0.25 mg/mL Cl-F-BBA completely halted mycelial growth and spore germination. Furthermore, a slightly lower concentration of 0.20 mg/mL was sufficient to compromise the integrity of the plasma membrane in mycelia and mitochondria, leading to a reduction in respiratory rate, activities of malate dehydrogenase (MDH), and succinate dehydrogenase (SDH), ATP content, and energy charge. This concentration also significantly disordered antioxidant metabolism, resulting in the accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA), and caused intracellular leakage in mycelia. In vivo experiments further demonstrated that Xiaozhou mustard root tubers treated with Cl-F-BBA exhibited markedly lower decay rates and lesion diameters compared to the control group. In summary, Cl-F-BBA presents a promising solution for controlling root tuber sour rot in Xiaozhou mustard caused by G. candidum.

Assessment of suitable region of Asparagus cochinchinensis (Lour.) Merr. under different climatic conditions in China by the MaxEnt model and HPLC analysis.

Asparagus cochinchinensis is a member of the Asparagaceae family whose medicinal part is the dried root tuber. The distribution of A. cochinchinensis and its secondary metabolites are closely associated with environmental factors, such as climate and soil properties. By establishing and optimizing a maximum entropy model, we analyzed and predicted the distribution pattern and migration direction of suitable habitats for A. cochinchinensis and determined the main environmental factors affecting the accumulation of secondary metabolites. Under current climatic conditions, the area of suitable habitats for A. cochinchinensis (208.38 × 104 km2) accounts for 21.71% of the land area of China, and the areas of lowly, moderately, and highly suitable areas were 64.15 × 104 km2, 113.66 × 104 km2, and 30.57 × 104 km2, respectively. Under future climate scenarios, the total area of suitable habitats hardly changes. The area of highly suitable habitats significantly decreases under the SSP1-RCP2.6 scenario (to 83.22% of the current value) and the SSP3-RCP7 scenario (to 48.94% of the current value), but eventually increases to 112.86% of the current value under the SSP5-RCP8.5 scenario, which indicates that A. cochinchinensis might adapt better to a high-carbon-emissions scenario. Under different climate scenarios, low-impact areas mainly occur in southern China and will correspond 92.07% of the current suitable area. Highly suitable habitats primarily occur in the southeastern Sichuan Basin, northern Guangxi, eastern Guizhou, and western Hunan. HPLC analysis showed that the content of protodioscin (0.373%) and protogracillin (0.044%) in S2 was the highest. The total saponins contents of S1 and S2 were the highest, which were 35.6586 and 33.1262 mg/g, respectively. The total polysaccharide content of S9 was the highest (16.9467%). The total contents of saponins and polysaccharides in A. cochinchinensis were significantly, but oppositely, correlated with temperature, precipitation, and other factors. This study has identified environmental factors affecting the growth and quality of A. cochinchinensis, which has guiding significance for resource conservation and site selection for large-scale cultivation.

The Impact of Nitrogen Levels on the Growth, Development, and Yield of Orange-fleshed Sweet Potatoes (Ipomoea batatas L.)

Nitrogen (N) is one of the most important fertilizers in agriculture because it promotes the growth of plants and the uptake of other plant nutrients. This nutrient plays a significant role in determining the yield and nutrient composition of sweet potato root tubers. Therefore, this study aimed to determine the optimum application rate of N in sweet potatoes to maximize yields and increase growth efficiency. Four levels of N (50, 100, 150, and 200 kg/ha) were applied as treatments. A randomized complete block design was used, and each treatment was replicated five times. Vine length, leaf length, stem thickness, and chlorophyll content were measured weekly, and the storage root yield was determined at the end of the experiment. The results showed a significant effect of the N treatments on plant growth, chlorophyll content, vine length, leaf area index and storage root yield. The chlorophyll content and vine length increased with an increase in the N rate. However, an inverse relationship was observed between storage root yield and N rates; the highest yield was recorded for the 50 kg/ha N treatment and the lowest yield was recorded for the 200 kg/ha N applied. Therefore, a rate of 50 to 100 kg/ha N is recommended for the production of orange-fleshed sweet potatoes.