In this paper, we examine the role of gravity waves (GWs) generated from tropospheric convection in initiating multiple nighttime ionospheric anomalies over Srinagar, located at 34.1°N, 74.8°E, and 25.91°N MLAT. Optical airglow observations during a geomagnetic quiet night (Ap = 4) of 29–30 June 2021, show the presence of plasma depletion tilted by an angle of ∼14.9° from the geographic North, quasi-periodic south eastward moving wave (QPSEMW), onset of two electrified MSTIDs and generation of plasma depletion channel between two MSTID phase fronts at ∼250 km altitude. GWs originating from deep convection in troposphere are detected at ∼85 and ∼97 km altitudes in OH and 557 nm filters respectively. The GWs in both the filters are propagating northward with almost similar characteristics. The intrinsic time period and vertical wavelength of GWs is estimated to be ∼11 min and ∼53 km respectively. We also present the observational indications of localised upliftment of F-layer ionosphere by ∼38 km, possibly initiated by polarization electric field generated through secondary GWs. The penetration of secondary GWs, generated by the dissipation of convective GWs, beyond the altitude of 500 km is also seen. FORMOSAT-7/COSMIC-2 RO data and SAMI3 model electron density suggest the occurrence of Sporadic-E (Es) layer at around 100 km altitude, caused by the negative gradient in eastward wind.