• Spectra of savanna understorey vegetation species over phenological seasons. • Separability of functional groups, and species, depended on season. • Informs species separation and timing for drone-based hyperspectral captures. • VNIR drone spectral range excludes foliar traits such as cellular water absorption. The relationship between species phenology and spectral separability is essential to determine the optimal remote sensing sampling period to maximise spectral separability of vegetation species. However, this relationship in many native grasses, introduced grasses and herbs, shrubs, and vine species in tropical savannas is unknown. We measured the in-situ hyperspectral response of monospecific vegetation stands of several understorey species representing different functional groups over phenological stages (throughout dry and wet seasons) using a non-imaging spectrometer. We present a spectral library of both native and introduced species of a tropical savanna environment. We analysed the data using continuum removal to highlight absorption features. Most understorey species displayed a photosynthetic spectral response with increased greenness at the end of the wet season that progressively declined as vegetation dried out. For some species, there were seasonally dependent differences in absorption features with spectral differences between the late wet and early dry season, and late dry and early wet seasons. We resampled the data to the spectral range typical of drone-mounted hyperspectral sensors (i.e., 150 bands between 400 and 1000 nm), which omits the water absorption features of the SWIR. These findings suggest an ideal sampling period for measuring outdoor canopy reflectance of understorey species, which will promote methodological improvements of hyperspectral data capture. The use of a VNIR only hyperspectral sensor will exclude the important regions of the spectrum for plant spectral identification including non-pigment bands for water, nitrogen and cellulose. The results have implications for determining the completeness of rehabilitation assessing reestablishment among indigenous species across mine sites under rehabilitation.