This study investigates and reviews methods for the assessment of the terrestrial bioaccumulation potential of hydrocarbons and related organic substances. The study concludes that the unitless biomagnification factor (BMF) and/or trophic magnification factor (TMF) are appropriate, practical, and thermodynamically meaningful metrics for identifying bioaccumulative substances in terrestrial food-chains. The study shows that various methods, including physical-chemical properties like the KOA and KOW , in-vitro biotransformation assays, quantitative structure activity relationships, in-vivo pharmacokinetic and dietary bioaccumulation tests as well as field based trophic magnification studies can inform on whether a substance has the potential to biomagnify in a terrestrial food-chain as defined by a unitless BMF exceeding 1. The study further illustrates how these methods can be arranged in a 4-tier evaluation scheme for the purpose of screening assessments that aim to minimize effort and costs and expediate bioaccumulation assessment of the vast numbers of organic substances in commerce; identifies knowledge gaps; and provides recommendations for further research to improve bioaccumulation assessment. This article is protected by copyright. All rights reserved. Integr Environ Assess Manag 2023;00:0-0. © 2023 SETAC.