An efficient and straightforward three-component reaction of alkynes (terminal and internal), elemental sulfur, and amides using novel bio-nanocomposite is described. The bio-nanostructure obtained from step-by-step coating the titanomagnetite core by silica, N,N,N-tributyltryptophanium iodide ionic liquid, and cobalt respectively. The Fe3-xTixO4-SiO2@TrpBu3+I−-Co(II) characterized though FT-IR, FESEM, EDAX, XRF, TGA/DTG, VSM, and XPS techniques. Its catalytic efficacy examined successfully in the solvent-free three-component reaction of alkynes (terminal and internal), S8, and amides to prepare the corresponding aryl thioamides via the CC triple bond cleavage. The reusability examination of the Fe3-xTixO4-SiO2@TrpBu3+I−-Co(II) bio-nanocomposite proceeded successfully within two runs. The recovered nanostructure characterized through EDAX and FESEM techniques that demonstrated no significant changes in the morphology and constitutive elements.