Personal Care Products Triclosan Research Articles

Self-assembled aminothiols: Platforms for ultra-sensitive electrochemical detection of triclosan via surface azo coupling

Extensive use of triclosan (TCS) in commercially available personal and health care products has led to its abundance in environment (water, soil), resulting in adverse impact on aquatic, marine, animal and human life. The present work is intended towards the development of simple and fast electrochemical sensors for trace level detection of TCS in aqueous media. The working of sensors is based on surface diazotization of self-assembled monolayers (SAMs) of two aminothiols, 4-aminothiophenol (4-ATP) and 5-amino-2-mercaptobenzimidazole (AMB) on polycrystalline gold electrodes. Using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), self-assembly of two aminothiols was confirmed as both offered significant impedance to [Fe(CN)6]3-/4− redox probe. The exposed amino groups of two self-assembled aminothiols were subjected to diazotization and diazo derivatives so formed were utilized as platform for impedimetric sensing of TCS through azo coupling. The step-by-step study of self-assembly, diazotization and azo coupling was conducted using different techniques such as FTIR, UV-visible, NMR, FESEM and EDX. The proposed ATP and AMB based EIS sensors exhibited sensitivity towards TCS in wide linear concentration range of 1.0 × 10-10 M – 1.0 × 10-2 M and 1.0 × 10-12 M – 1.0 × 10-4 M with lower detection limits of 5.6 × 10-11 M and 7.9 × 10-13 M, respectively. A good selectivity was observed in the presence of common metal ions (Na+, K+, Ca2+, Mg2+, Cu2+, Fe3+, Zn2+, Co2+) and surfactants (SDS, Triton X-100, Triton X-114). Using proposed EIS sensors, the estimation of TCS in personal care products (soap, facewash, handwash) and water samples (surface and groundwater) has been successfully achieved.

In-situ formation of hydrophobic deep eutectic solvent for the enrichment and quantitative determination of triclosan in personal care products and environmental water samples

Green techniques for sample pretreatment and quantitative analysis are important for environmental concerns. In this work, a new liquid–liquid microextraction strategy is developed by in-situ formation of hydrophobic deep eutectic solvents (DESs) where a contaminant (such as triclosan) serves as a hydrogen bond acceptor and a hydrophobic compound (such as 2,2,4-trimethyl-1,3-pentanediol) acts as a hydrogen bond donor. This strategy, along with high performance liquid chromatography, is used to establish the method for enrichment and quantitative determination of trace triclosan from real samples. For this purpose, the factors affecting the extraction and determination were investigated by using single-factor test. The result showed that the optimal extraction condition was, extraction temperature 45 °C, the amount of extractant 150 mg, the pH of solution 5.9, the volume of diluent (methanol) 100 µL, the concentration of salt (NaCl), 1.00 mol/L. Under the optimum conditions, the linear range of the proposed method was 1.7–1000 µg/L with the linear correlation coefficient (R2) of 0.9991. The detection limit and quantitation limit were 0.5 and 1.7 µg/L, respectively. Finally, this method was used to determinate triclosan content in the leach solution of personal care products and environmental water samples, including facial cleanser, soap, toothpaste, hand sanitizer, tap water, surface water, rainwater, lake water, and wastewater of sewage treatment plant. The extraction recovery of triclosan was between 76 %−115 %, and the relative standard deviation was less than 7.36 %. These results indicate that the proposed method was effective and practical for the enrichment and quantitative determination of trace triclosan in personal care products and environmental water samples.

In-Situ Formation of Hydrophobic Deep Eutectic Solvent for the Enrichment and Quantitative Determination of Triclosan in Personal Care Products from Environmental Samples

In-Situ Formation of Hydrophobic Deep Eutectic Solvent for the Enrichment and Quantitative Determination of Triclosan in Personal Care Products from Environmental Samples

Triclosan affects motor function in zebrafish larva by inhibiting ache and syn2a genes

The widespread use of triclosan in personal care products as an antimicrobial agent is leading to its alarming tissue-bioaccumulation including human brain. However, knowledge of its potential effects on the vertebrate nervous system is still limited. Here, we hypothesized that sublethal triclosan concentrations are potent enough to alter motor neuron structure and function in zebrafish embryos exposed for prolonged duration. In this study, zebrafish embryos were used as vertebrate-animal model. Prolonged exposure (up to 4 days) of 0.6 mg/L (LC50, 96 h) and 0.3 mg/L (<LC50, Sublethal) triclosan produced aberrations in motor neuron innervations in skeletal muscles and reduced touch-evoked escape response in zebrafish larvae. This suggests motor dysfunction in treated embryos. To further explore the mechanisms of triclosan induced neurotoxicity, we determined the enzyme activity of acetylcholinesterase (AChE) and the expression of acetylcholinesterase (ache), myelin basic protein (mbp) and synapsin IIa (syn2a) genes which play an important role in the neural development and synaptic transmission. The ache and syn2a genes were down-regulated in triclosan treated larvae without any significant changes in mbp gene expression. At functional level, we observed a decrease in the AChE activity. Furthermore, docking results showed that triclosan can form a stable interaction with binding pocket of AChE and perhaps it can compete with natural acetylcholine for direct binding to AChE thereby inhibiting it and affecting cholinergic transmission. Therefore, triclosan can be regarded as a neurotoxic agent even at sublethal concentrations. Overall, the growing toxicological evidence against triclosan including ours suggest caution in its widespread use.

The effect of temperature on Triclosan and Lead exposed mussels.

Interest on the effects of emerging contaminants over aquatic organisms has increased in the last years. Nonetheless, the toxic action of classical natural and anthropogenically-driven metals has also to be monitored, especially because they reflect real environmental situations. For that, in the present study we focused on the effects on the marine mussel Mytilus galloprovincialis of the personal care product Triclosan (TCS) and Lead (Pb), as toxic metal, under separate and co-exposure situations at environmentally relevant concentrations: TCS (1 μg/L) and Pb (50 μg/L). The consideration of an additional factor such as an increase in ambient temperature was also included to provide a forecasted scenario of climate change: from the ambient temperature at actual conditions (17 °C) to a predicted warming situation (22 °C). Water chemical characterization and some physical properties and bioaccumulation of TCS and Pb in mussels at the end of the experiment (28 days) was considered. The parameters followed up comprise the energy related system production (electron transport system) and glycogen and protein reserves. Antioxidant enzymatic defences towards reactive oxygen species (ROS) and the consequences of ROS damage over endogenous lipids (LPO) and proteins (PC). Overall the results suggested only particular responses to chemical exposures at 17 °C whereas at 22 °C the detoxification machinery was set up and this prevented the occurrence of LPO. Nonetheless, PC formation occurred under Pb and TCS + Pb co-exposure at the highest temperature. Due to the complexity of the study: 4 chemical conditions, 2 temperatures and 10 biomarkers considered, a principal component ordination (PCO) analysis was included. The results of this integrative analysis confirmed a clear effect of the temperature, more responsiveness to drugs at 22 °C and in all likelihood due to Pb presence.

Fate and impacts of pharmaceuticals and personal care products after repeated applications of organic waste products in long-term field experiments

Recycling organic waste products in agriculture is a potential route for the dispersion of pharmaceutical residues in the environment. In this study, the concentrations of thirteen pharmaceuticals and the personal care product triclosan (PPCPs) were determined in different environmental matrices from long-term experimental fields amended with different organic waste products (OWPs), including sludge, composted sludge with green wastes, livestock effluents and composted urban wastes applied at usual agricultural rates.PPCP concentrations were different in OWPs, varying from a few micrograms to milligrams per kilogram dry matter or per litre for slurry. OWPs from sludge or livestock effluents primarily contained antibiotics, whereas composted urban wastes primarily contained anti-inflammatory compounds.PPCP contents in soils amended for several years were less than a few micrograms per kilogram. The most persistent compounds (fluoroquinolones, carbamazepine) were quantified or detected in soils amended with sludge or composted sludge. In soils amended with composted municipal solid waste, carbamazepine was quantified, and fluoroquinolones, ibuprofen and diclofenac were sometimes detected. The small increases in fluoroquinolones and carbamazepine in soils after individual OWP applications were consistent with the fluxes from the applied OWP. The measured concentrations of pharmaceuticals in soil after several successive OWP applications were lower than the predicted concentrations because of degradation, strong sorption to soil constituents and/or leaching. Dissipation half-lives (DT50) were approximately 750–2500, 900 and <300days for fluoroquinolones, carbamazepine and ibuprofen, respectively, in temperate soils and <350 and <80days for fluoroquinolones and doxycycline, respectively, in tropical soils.Detection frequencies in soil leachates were very low (below 7%), and concentrations ranged from the limits of detection (0.002–0.03μg/L) and exceptionally to 0.27μg/L. The most frequently detected pharmaceuticals were carbamazepine and ibuprofen.Based on the risk quotient, the estimated ecotoxicological risks for different soil organisms were low.

Use the Extended Activated Sludge Process to Remove Triclosan (TCS) from Wastewater Treatment Plant

The antibacterial personal care product Triclosan (TCS), is a broad spectrum antibacterial. It has been used for more than 40 years as an antiseptic, disinfectant or preservative in clinical settings, and in the various consumer products including cosmetics, plastic materials and toys. It has lately been determined in the sources of the aquatic environment; its potential effect on ecosystems. The contaminants can reach the soil and aquatic environment through land application of wastewater effluent and agricultural runoff, and then reach to humans through the food chain. Triclosan is used worldwide on a large scale especially in many personal care products, like toothpaste, shampoo and soap. In this study, the removal of Triclosan was investigated in an extended activated sludge process set in a laboratory-scale reactor. Activated sludge biomass used in the reactor was obtained from Gaziantep wastewater treatment plant. The analytical method based on solid-phase extraction (SPE) followed by high Performance Liquid Chromatography (HPLC) was used for the determination of Triclosan in extended activated sludge effluent. Reactor was operated in different HRTs and Triclosan removal efficiency was obtained as 92.5%, 95.4%, 99.1% and 99.9% for different HRT values of 18h, 24h, 48h, and 52h, respectively.

In vitro interaction of emerging contaminants with the cytochrome p450 system of Mediterranean deep-sea fish.

The interactions of emerging contaminants with the xenobiotic and endogenous metabolizing system of deep-sea fish were compared. The drugs diclofenac, fluoxetine, and gemfibrozil belong to different pharmaceutical classes with diverse mechanistic actions, and the personal care products triclosan, galaxolide, and nonylphenol are representative of antibacterial agents, nitro-musks, and surfactants, respectively. The fish compared are representative of the middle and lower slope of deep-sea habitats. The species were adults of Trachyrynchus scabrus, Mora moro, Cataetix laticeps, and Alepocehalus rostratus. The hepatic metabolic system studied were the activities associated with several cytochrome P450 isoforms (CYPs): 7-ethoxyresorufin-O-deethylase (EROD), benzyloxy-4-[trifluoromethyl]-coumarin-O-debenzyloxylase (BFCOD), and 7-ethoxycoumarin-O-deethylase (ECOD). Results showed differences in baseline activities and sensitivity to chemicals which were species, chemical, and pathway dependent. T. scabrous was the most sensitive species to chemical interactions with the xenobiotic and endogenous metabolizing (EROD and BFCOD) systems, especially in the case of diclofenac interference with BFCOD activity (IC50 = 15.7 ± 2.2 μM). Moreover, T. scabrous and A. rostratus possessed high basal ECOD activity, and this was greatly affected by in vitro exposure to diclofenac in T. scabrous also (IC50 = 6.86 ± 1.4 μM). These results highlight the sensitivity of marine fish to emerging contaminants and propose T. scabrous (middle slope) and A. rostratus (lower slope) as sentinels and the inclusion of ECOD activity as a sensitive biomarker to these exposures.

Obtaining process mass balances of pharmaceuticals and triclosan to determine their fate during wastewater treatment

To better understand pharmaceutical fate during wastewater treatment, analysis in both aqueous and particulate phases is needed. Reported herein is a multi-residue method for the determination of ten pharmaceutical drugs and the personal care product triclosan in wastewater matrices. Method quantitation limits ranged from 7.6 to 76.6ngl−1 for aqueous phases and from 7.0 to 96.7ngg−1 for particulate phases. The analytical method was applied to attain a complete process mass balance of a pilot-scale activated sludge plant (ASP) operated under controlled conditions. The mass balance (inclusive of aqueous and particulate concentrations at all sample points) was used to diagnose removal, revealing pharmaceuticals to be separable into three fate pathways: (a) biological degradation, (b) sorption onto activated sludge and (c) resistant to removal from the aqueous phase. These differences in fate behaviour explained a broad range of secondary removal observed (−8 to 99%). The ASP was also simultaneously compared to a full-scale trickling filter (TF) works whilst receiving the same influent wastewater. Performance of the ASP and TF was similar, achieving total pharmaceutical removals of 253 and 249μgg−1 biochemical oxygen demand (BOD) removed, respectively. This corresponded with reductions in total pharmaceutical load of 91 and 90% (ANOVA, p-value>0.05). Interestingly, despite low suspended solid concentrations final effluents of both the ASP and TF contained significant concentrations of some chemicals in the particulate phase. Individually, triclosan and the antibiotics ofloxacin and ciprofloxacin were within the particulate phase of effluents at concentrations ranging from 26 to 296ngl−1.

Toxicity of biosolids-derived triclosan and triclocarban to six crop species.

Biosolids are an important source of nutrients and organic matter, which are necessary for the productive cultivation of crop plants. Biosolids have been found to contain the personal care products triclosan and triclocarban at high concentrations relative to other pharmaceuticals and personal care products. The present study investigates whether exposure of 6 plant species (radish, carrot, soybean, lettuce, spring wheat, and corn) to triclosan or triclocarban derived from biosolids has an adverse effect on seed emergence and/or plant growth parameters. Plants were grown in soil amended with biosolids at a realistic agronomic rate. Biosolids were spiked with triclosan or triclocarban to produce increasing environmentally relevant exposures. The concentration of triclosan and triclocarban in biosolids-amended soil declined by up to 97% and 57%, respectively, over the course of the experiments. Amendment with biosolids had a positive effect on the majority of growth parameters in radish, carrot, soybean, lettuce, and wheat plants. No consistent triclosan- or triclocarban-dependent trends in seed emergence and plant growth parameters were observed in 5 of 6 plant species. A significant negative trend in shoot mass was observed for lettuce plants exposed to increasing concentrations of triclocarban (p<0.001). If best management practices are followed for biosolids amendment, triclosan and triclocarban pose a negligible risk to seed emergence and growth of crop plants.

A Survey of Alkylphenols, Bisphenols, and Triclosan in Personal Care Products from China and the United States

Exposure of humans to environmental phenolic compounds such as bisphenol A (BPA) and alkylphenols is a matter of concern, due to these compounds' ubiquitous occurrence and estrogenic potencies. Little is known about the levels of environmental phenolics in personal care products (PCPs). In this study, nonylphenol, two octylphenols, eight bisphenols (BPA and its analogs), and triclosan (TCS) were determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in PCP samples (n=231) collected from China and the United States (U.S.). The concentrations of 4-n-nonylphenol (4-NP), 4-n-octylphenol (4-OP), 4-tert-octylphenol (4-t-OP), and TCS were in the ranges of <0.5-39,100 [geometric mean (GM): 21.5], <0.5-315 (0.680), <1.0-10,100 (2.69), and <0.5-53,900 (3.03)ng/g, respectively. The GM concentrations of individual bisphenols, including BPA, bisphenol S (BPS), and bisphenol F (BPF), were generally at sub-nanogram per gram levels. No significant differences in concentrations of the target compounds were found among various PCP categories or between China and the U.S. The estimated GM daily intakes of 4-NP, ∑OPs (sum of 4-OP and 4-t-OP), ∑BPs (sum of eight bisphenols), and TCS through dermal absorption from the use of PCPs were 0.932, 0.093, 0.072, and 0.016μg/day, respectively, for adult Chinese women and 0.340, 0.054, 0.120, and 0.068μg/day, respectively, for adult U.S. women. Body lotions, face creams, and liquid foundations accounted for the majority (>85%) of the dermal exposure doses of the target phenolics.

Application of a non-aqueous capillary electrophoresis method to the analysis of triclosan in personal care products

The application of a non-aqueous capillary electrophoresis method to the analysis of triclosan in personal care products.

Rapid determination of triclosan in personal care products using new in-tube based ultrasound-assisted salt-induced liquid–liquid microextraction coupled with high performance liquid chromatography-ultraviolet detection

This paper describes the development of a novel, simple and efficient in-tube based ultrasound-assisted salt-induced liquid–liquid microextraction (IT-USA-SI-LLME) technique for the rapid determination of triclosan (TCS) in personal care products by high performance liquid chromatography-ultraviolet (HPLC-UV) detection. IT-USA-SI-LLME method is based on the rapid phase separation of water-miscible organic solvent from the aqueous phase in the presence of high concentration of salt (salting-out phenomena) under ultrasonication. In the present work, an indigenously fabricated home-made glass extraction device (8-mL glass tube inbuilt with a self-scaled capillary tip) was utilized as the phase separation device for USA-SI-LLME. After the extraction, the upper extractant layer was narrowed into the self-scaled capillary tip by pushing the plunger plug; thus, the collection and measurement of the upper organic solvent layer was simple and convenient. The effects of various parameters on the extraction efficiency were thoroughly evaluated and optimized. Under optimal conditions, detection was linear in the concentration range of 0.4–100ngmL−1 with correlation coefficient of 0.9968. The limit of detection was 0.09ngmL−1 and the relative standard deviations ranged between 0.8 and 5.3% (n=5). The applicability of the developed method was demonstrated for the analysis of TCS in different commercial personal care products and the relative recoveries ranged from 90.4 to 98.5%. The present method was proven to be a simple, sensitive, less organic solvent consuming, inexpensive and rapid procedure for analysis of TCS in a variety of commercially available personal care products or cosmetic preparations.

Slow O-demethylation of methyl triclosan to triclosan, which is rapidly glucuronidated and sulfonated in channel catfish liver and intestine

The antibacterial personal care product triclosan is discharged in municipal waste, and converted in part by bacteria in sewage sludge and soil to its more lipid-soluble methyl ether, methyl triclosan. Triclosan and methyl triclosan have been detected in water, sediment, fish and invertebrates near sewage treatment facilities. Understanding the biotransformation of methyl triclosan and triclosan in a model food fish, the channel catfish, will be of value in assessing the likelihood that these compounds will bioaccumulate in exposed fish, and therefore potentially pass up the food chain. We hypothesize that cytochrome P450 will catalyze the O-demethylation of methyl triclosan to yield triclosan, which is likely to undergo glucuronidation or sulfonation of the phenolic hydroxyl group. Conversion of methyl triclosan to triclosan was measured by LC/MS/MS following aerobic incubation of varying concentrations of methyl triclosan with NADPH and hepatic and intestinal microsomes from untreated, 3-methylcholanthrene-treated (10mg/kg, i.p.) or PCB-126-treated (0.1mg/kg, i.p.) channel catfish (n=4 per treatment group). The Km values for methyl triclosan were similar for untreated, 3-methylcholanthrene-treated and PCB-126-treated catfish liver microsomes, ranging from 80 to 250μM. Vmax values for O-demethylation ranged from 30 to 150pmol/min/mg protein, with no significant differences between controls, PCB-126-treated or 3-methylcholanthrene-treated fish, suggesting that methyl triclosan O-demethylation was not a CYP1-catalyzed reaction. Methyl triclosan O-demethylation activities in intestinal microsomes were similar to or lower than those found with liver microsomes. The calculated rate of O-demethylation of methyl triclosan in catfish liver at 1μM, a concentration reported in exposed fish, and 21°C, an early summer water temperature, is 0.10pmol/min/mg protein. This slow rate of metabolism suggests that upon continued exposure, methyl triclosan may bioaccumulate in the channel catfish. Triclosan itself, however, was readily glucuronidated by hepatic and intestinal microsomes and sulfonated by hepatic and intestinal cytosol. Triclosan glucuronidation followed Michaelis-Menten kinetics when rates were measured across a concentration range of 5–1000μM, whereas triclosan sulfonation exhibited substrate inhibition at concentrations above 10–20μM in both intestinal and hepatic cytosol. Based on the enzyme kinetic constants measured in hepatic and intestinal fractions at 21°C, triclosan at 1μM could be glucuronidated at rates of 23 and 3.2pmol/min/mg protein respectively in liver and intestine, and sulfonated at rates of 277 (liver) and 938 (intestine) pmol/min/mg protein. These rates are much higher than the rates of demethylation of methyl triclosan, and suggest that triclosan would be rapidly cleared and unlikely to bioaccumulate in catfish tissues

Pharmaceutical and personal care products in tile drainage following surface spreading and injection of dewatered municipal biosolids to an agricultural field

Land application of municipal biosolids can be a source of environmental contamination by pharmaceutical and personal care products (PPCPs). This study examined PPCP concentrations/temporally discrete mass loads in agricultural tile drainage systems where two applications of biosolids had previously taken place. The field plots received liquid municipal biosolids (LMB) in the fall of 2005 at an application rate of ∼ 93,500 L ha − 1 , and a second land application was conducted using dewatered municipal biosolids (DMB) applied at a rate of ∼ 8 Mt dw ha − 1 in the summer of 2006. The DMB land application treatments consisted of direct injection (DI) of the DMB beneath the soil surface at a nominal depth of ∼ 0.11 m, and surface spreading (SS) plus subsequent tillage incorporation of DMB in the topsoil (∼ 0.10 m depth). The PPCPs examined included eight pharmaceuticals (acetaminophen, fluoxetine, ibuprofen, gemfibrozil, naproxen, carbamazepine, atenolol, sulfamethoxazole), the nicotine metabolite cotinine, and two antibacterial personal care products triclosan and triclocarban. Residues of naproxen, cotinine, atenolol and triclosan originating from the fall 2005 LMB application were detected in tile water nearly nine months after application (triclocarban was not measured in 2005). There were no significant differences ( p > 0.05) in PPCP mass loads among the two DMB land application treatments (i.e., SS vs. DI); although, average PPCP mass loads late in the study season (> 100 days after application) were consistently higher for the DI treatment relative to the SS treatment. While the concentration of triclosan (∼ 14,000 ng g − 1 dw) in DMB was about twice that of triclocarban (∼ 8000 ng g − 1 dw), the average tile water concentrations for triclosan were much higher (43 ± 5 ng L − 1 ) than they were for triclocarban (0.73 ± 0.14 ng L − 1 ). Triclosan concentrations (maximum observed in 2006 ∼ 235 ng L − 1 ) in tile water resulting from land applications may warrant attention from a toxicological perspective.

Spectrophotometric determination of triclosan in personal care products

A spectrophotometric method for the determination of triclosan in personal care products was proposed. It was based on the reaction of sodium nitrite with p-sulfanilic acid in an acidic medium to form diazonium ion, with which triclosan further formed an azo compound in an alkaline medium. The resulting yellow colored product has a maximum absorption at 452 nm. A good linear relationship ( r = 0.9999) was obtained in the range of 0–30 mg L −1 triclosan. A detection limit of 0.079 g L −1 was achieved and the relative standard deviation was 0.24% ( n = 11) at 14 mg L −1 triclosan. The proposed method has been applied to the analyses of triclosan in several personal care products and the results were in good agreement with those obtained by high-performance liquid chromatography.

Triclosan is a potent inhibitor of estradiol and estrone sulfonation in sheep placenta

The personal care product Triclosan, 5-chloro-2(2,4-dichlorophenoxy)-phenol, is widely used in consumer products as an antibacterial agent and is increasingly found in the environment as a contaminant of sewage sludge and wastewater. This compound has been identified in plasma and urine of people in the United States, Sweden and Australia. Triclosan is known to inhibit sulfonation of phenolic xenobiotics and is structurally related to inhibitors of estrogen sulfotransferase, such as polychlorobiphenylols. In pregnancy, the placenta is an important source of estrogen, which is needed for normal fetal development and successful parturition, and estrogen sulfotransferase is thought to play an important role in regulation of estrogen availability. In this study, we examined the effect of Triclosan on sheep placental cytosolic sulfotransferase activity with 17-beta-estradiol and estrone as substrates. For comparison, we studied the effects of 4-hydroxy-3,3',4',5-tetrachlorobiphenyl and 2'-hydroxytriclocarban on estradiol sulfonation. The apparent K m for placental cytosolic sulfotransferase activity with estradiol as substrate was 0.27 ± 0.06 nM (mean ± S.D., n = 3 individuals) and with estrone as substrate was 1.86 ± 0.22 nM. Partial substrate inhibition was observed with estradiol at concentrations higher than 10–20 nM, as is typical of estrogen sulfotransferases (SULT1E1) in other species. Studies of the effect of Triclosan on estrogen sulfotransferase activity were conducted with several concentrations (0.1–6 nM) of estradiol and with 2 nM estrone. Triclosan was a very potent inhibitor of both estradiol and estrone sulfonation. For estradiol the inhibition was shown to be mixed competitive/uncompetitive, with K ic of 0.09 ± 0.01 nM and K iu of 5.2 ± 2.9 nM. The IC 50 for inhibition of estrone sulfonation was 0.60 ± 0.06 nM. At an environmentally relevant concentration of 1 µM, Triclosan was not a substrate for glucuronidation in sheep placental microsomes. Triclosan could be sulfonated in placental cytosol with K m 1.14 ±0.18 µM and V max 160 ± 26 pmol/min/mg protein, however the calculated rates of Triclosan sulfonation were negligible at the low nM concentrations that potently inhibit estrogen sulfonation. The high potency of Triclosan as an inhibitor of estrogen sulfotransferase activity raises concern about its possible effects on the ability of the placenta to supply estrogen to the fetus, and in turn on fetal growth and development.

New approach on trace analysis of triclosan in personal care products, biological and environmental matrices

Stir bar sorptive extraction and liquid desorption followed by high performance liquid chromatography with diode array detection (SBSE-LD-LC-DAD) is proposed for the determination of triclosan in personal care products, biological and environmental matrices, which is included in the priority lists, set by several international regulatory organizations. Instrumental conditions and experimental parameters that affecting SBSE-LD efficiency are fully discussed. Throughout systematic assays on 25 mL water samples spiked at the 10.0 μg L −1 level, it had been established that stir bars coated with 126 μL of polydimethylsiloxane, an equilibrium time of 1 h (1000 rpm) and acetonitrile under sonification (60 min) as back-extraction solvent, allowed the best analytical performance to determine triclosan in water matrices. From the data obtained, good recovery and remarkable repeatability were attained, providing experimental average yields (78.5 ± 2.2%), although slightly lower than the theoretical equilibrium (99.7%) described by the octanol–water partition coefficients ( K PDMS/W < K O/W). The analytical performance proved suitable precision (<3.6%), convenient detection limits (0.1 μg L −1) and excellent linear dynamic range ( r 2 > 0.9992) from 0.4 to 108.0 μg L −1. The application of the present method to determine triclosan in real matrices such as commercial toothpaste, saliva and urban wastewater samples, allowed appropriate selectivity, high sensitivity and accuracy using the standard addition methodology. The proposed method showed to be feasible and sensitive with a low-sample volume requirement to monitor triclosan in personal care products, biological and environmental matrices at the trace level, in compliance with international regulatory directives.