Trichosporon Research Articles

Adhesion and biofilm formation by two clinical isolates of Trichosporon Cutaneum in various environmental conditions.

Trichosporon spp. is an emerging opportunistic pathogen and a common cause of both superficial and invasive infections. Although Trichosporon asahii is the most frequently isolated species, Trichosporon cutaneum is also widely observed, as it is the predominant agent in cases of white Piedra and onychomycosis. Trichosporon spp. is a known to produce biofilms, which serve as one of its virulence mechanisms, however, there is limited data available on biofilms formed by T. cutaneum. Thus, the aim of this study was to assess the adhesion and biofilm formation of two clinical isolates of T. cutaneum under various environmental conditions (including temperature, nutrient availability, and carbon source), as well as their tolerance to fluconazole. Adhesion was tested on common abiotic substrates (such as silicone, glass, and stainless steel), revealing that T. cutaneum readily adhered to all surfaces tested. CV staining was applied for the evaluation of the environment influence on biofilm efficiency and it was proved that the nutrient availability has a major impact. Additionaly, fluorescent staining was employed to visualize the morphology of T. cutaneum biofilm and its survival in the presence of fluconazole. Hyphae production was shown to play a role in elevated biofilm production in minimal medium and increased tolerance to fluconazole.

Production of single cell protein from brewer’s spent grain through enzymatic saccharification and fermentation enhanced by ammoniation pretreatment

Brewer’s spent grain (BSG) is a major low-value by-product of beer industry. To realize the high value application of BSG, this work proposed a strategy to produce single cell protein (SCP) with oligosaccharide prebiotics from BSG, via ammoniation pretreatment, enzymatic hydrolysis, and fermentation. The optimum conditions of ammoniation pretreatment obtained by response surface method were 11 % ammonia dosage (w/w), 63 °C for 26 h. Suitable enzyme and yeast were screened to enhance the conversion of cellulose and hemicellulose in BSG into sugars and maximize the SCP yield. It was shown that using lignocellulolytic enzyme SP from Penicillium oxalicum and Trichosporon cutaneum, about 310 g of SCP with 80 g of arabinoxylo-oligosaccharides were obtained from 1000 g of BSG. This process is low cost, high efficiency, and easy to implement, which has good industrial application prospects.

Simultaneous enhancement of lignin-derived inhibitor tolerance and lipid accumulation of oleaginous yeast Trichosporon cutaneum by adaptive evolution

Inhibitory compounds should be removed from the pretreated lignocellulose feedstock before fermentative production of microbial lipid by oleaginous yeast. Biodetoxification effectively degrades furan aldehydes and weak organic acids, but is less efficient against complex and various lignin-derived phenolic aldehydes. Residual phenolics in lignocellulose hydrolysates require a tolerant fermentation strain. This study adopted the long-term adaptive evolution combined with centrifugal enrichment in corn stover hydrolysate to simultaneously improve the phenolic inhibitors tolerance and lipid synthesis of Trichosporon cutaneum. The obtained mutant T. cutaneum MS28 showed significant improved performances of phenolic aldehydes tolerance and lipid accumulation. The final cellulosic lipid production of T. cutaneum MS28 utilizing corn stover reached 33.8 ± 0.1 g/L, approximately 6-fold higher than that of the starting strain. Transcriptional analysis suggested that T. cutaneum MS28 had more active lignocellulose-derived sugars metabolism and lipid synthesis shunts. The intracellular contents of lipid synthesis precursors including NADPH and acetyl-CoA increased 1.7–3.0 folds. This adaptive evolution in lignocellulose hydrolysate combined with centrifugal enrichment is an efficient tool to targeted enhancement of inhibitors tolerance and cellulosic lipid production of oleaginous yeast.

Biodegradation and Utilization of the Pesticides Glyphosate and Carbofuran by Two Yeast Strains

The widespread use of synthetic pesticides in agricultural practice is associated with the risk of environmental pollution, damage to non-target organisms, and harm to the health of consumers due to the presence of pesticides in the resulting products. Metabolically active microbial species play a significant role in eliminating harmful chemicals from the environment. The two yeast strains used in this study are Trichosporon cutaneum R57 and Candida tropicalis V1. Both strains showed growth and development in the presence of the pesticides glyphosate and carbofuran used as the sole carbon sources. The gas-chromatographic analysis performed showed that C. tropicalis V1 reached 76% of 0.3 g/L glyphosate biodegradation in 192 h. At the same time, the amount of glyphosate decreased by about 58% in the culture of T. cutaneum R57. During the glyphosate biodegradation process, the presence of the intermediate metabolites methylglycine and glycine was revealed. Strain T. cutaneum R57 demonstrated nearly total biodegradation of 0.3 g/L carbofuran in 192 h of cultivation. Strain C. tropicalis V1 showed a slow decrease (23.4%) of the same amount of carbofuran during 192 h. Pyruvic acid and carbofuran-7-phenol have been found to be intermediate metabolites in the breakdown of carbofuran. This report provides new information on the potential of yeasts to participate in environmental pesticide cleanup processes.

Influence of sampling location and processing on the assembly and network of Polygoni Multiflori Radix surface microbiome

The raw and processed roots of Polygonum multiflorum Thunb is a popular traditional Chinese medicine. However, Polygoni Multiflori Radix is easily contaminated by toxigenic fungi and mycotoxins during harvesting, processing, and transportation, thereby posing a health risk for consumers. This study aims to investigate the presence of fungi on the surface of raw and processed Polygoni Multiflori Radix collected from four producing areas using high-throughput sequencing. Results showed that the phyla Ascomycota and Basidiomycota, the genera Xeromyces, Cystofilobasidium, Eurotium, and Aspergillus were the dominant fungus, and significant differences are presented in four areas and two processed products. Three potential mycotoxin-producing fungi were detected, namely Trichosporon cutaneum, Aspergillus restrictus, and Fusarium oxysporum. The α-diversity and network complexity showed significant differences in four areas. Chao 1 and Shannon were highest in Yunnan (YN), then incrementally decreased from SC (Sichuan) to AH (Anhui) and GD (Guangdong) areas. Meanwhile, α-diversity was also strongly influenced by processing. Chao 1 and Shannon indices were higher in the raw group, however, the network complexity and connectivity were higher in the processed group. In conclusion, the assembly and network of the surface microbiome on Polygoni Multiflori Radix were influenced by sampling location and processing. This work provides details on the surface microbiome of Polygoni Multiflori Radix samples, which could ensure the drug and consumers' safety.

Simultaneous bioreduction of tellurite and selenite by Yarrowia lipolytica, Trichosporon cutaneum, and their co-culture along with characterization of biosynthesized Te–Se nanoparticles

BackgroundNatural and anthropogenic activities, such as weathering of rocks and industrial processes, result in the release of toxic oxyanions such as selenium (Se) and tellurium (Te) into the environment. Due to the high toxicity of these compounds, their removal from the environment is vital.ResultsIn this study, two yeast strains, Yarrowia lipolytica and Trichosporon cutaneum, were selected as the superior strains for the bioremediation of tellurium and selenium. The reduction analyses showed that exposure to selenite induced more detrimental effects on the strains compared to tellurite. In addition, co-reduction of pollutants displayed almost the same results in selenite reduction and more than ~ 20% higher tellurite reduction in 50 h, which shows that selenite triggered higher tellurite reduction in both strains. The selenite and tellurite kinetics of removal were consistent with the first-order model because of their inhibitory behavior. The result of several characterization experiments, such as FE-SEM (Field emission scanning electron microscopy), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), X-ray diffractometer (XRD), and dispersive X-ray (EDX) on Te–Se nanoparticles (NPs) revealed that the separated Te–Se NPs were needle-like, spherical, and amorphous, consisted of Te–Se NPs ranging from 25 to 171 nm in size, and their surface was covered with different biomolecules.ConclusionsRemarkably, this work shows, for the first time, the simultaneous bioreduction of tellurite and selenite and the production of Te–Se NPs using yeast strains, indicating their potential in this area, which may be applied to the nanotechnology industry and environmental remediation.Graphical

Novel supramolecular deep eutectic solvent-enabled in-situ lignin protection for full valorization of all components of wheat straw

Lignin is usually deemed as an inhibitor to enzymatic hydrolysis of cellulose due to its physical barrier, non-productive adsorption, and steric hindrance. Herein, a novel supramolecular deep eutectic solvent (SUPRADES), comprising ethylene glycol and citric acid in 5:1 M ratio, and β-cyclodextrin (β-CD) in a concentration of 3.5% (w/w), was developed to be efficient for pretreating wheat straw. The delignification rate, cellulose enzymatic digestibility, and hemicellulose removal reached 90.45%, 97.36% and 87.24%, respectively, which may be attributed to the introduction of β-CD with superior ability of both adsorption and in-situ lignin protection to efficiently remove lignin with intact structure from cellulose surface. The mechanisms of high-efficiency lignin extraction/protection were systematically illustrated by adsorption kinetics. Moreover, Trichosporon cutaneum grown on the hemicellulose and cellulose fractions after pretreatment afforded 8.8 g total lipids from 100 g wheat straw. The green SUPARDES pretreatment strategy offers a new avenue for upgrading lignocellulose to biofuels.

Coproduction of single cell protein and lipid from lignocellulose derived carbohydrates and inorganic ammonia salt with soluble ammonia recycling

Co-production of single cell protein (SCP) and lipid from lignocellulose-derived carbohydrates and inorganic ammonia offers a promising alternative for poultry or aquaculture feeds. An engineered oleaginous yeast Trichosporon cutaneum MP11 showed great potential for producing SCP and lipid from wheat straw and ammonia sulfate with minimum nutrient input. Trichosporon cutaneum MP11 showed stronger SCP and lipid fermentability using dry acid pretreated and biodetoxified wheat straw than using pure sugars. The residual ammonium sulfate in fermentation broth was recycled up to five times, resulting in ∼70% of nitrogen fixation into SCP. The overall yield of SCP and lipid from lignocellulose-derived sugars was 0.15 g/g and 0.11 g/g, respectively. This translates to the production of one ton of SCP (0.56 ton) and lipid (0.44 ton) from 6.6 tons of wheat straw, or one ton of SCP and lipid containing yeast cells (dry) from 4.8 tons of wheat straw.

Cellulase mediated stress triggers the mutations of oleaginous yeast Trichosporon cutaneum with super-large spindle morphology and high lipid accumulation.

Accumulation of intracellular lipid bodies in oleaginous yeast cells is highly restricted by their natural intracellular space. Here we show a cellulase mediated adaptive evolution with ultra-centrifugation fractionation of oleaginous yeast Trichosporon cutaneum to obtain the favorable cell structure for lipid accumulation. Cellulase was added to the wheat straw hydrolysate during long-term adaptive evolution for disruption of cell wall integrity of T. cutaneum cells. The cellulase, together with ultracentrifugation force, triggered multiple mutations and transcriptional expression changes of the functional genes associated with cell wall integrity and lipid synthesis metabolism. The fractionated mutant T. cutaneum YY52 demonstrated the heavily weakened cell wall and high lipid accumulation by the super-large expanded spindle cells (two orders of magnitude greater than the parental). A record-high lipid production by T. cutaneum YY52 was achieved (55.4±0.5gL-1 from wheat straw and 58.4±0.1gL-1 from corn stover). This study not only obtained an oleaginous yeast strain with industrial application potential for lipid production but also provided a new method for generation of mutant cells with high intracellular metabolite accumulation.

Efficient delignification of wheat straw for microbial lipid production enabled by a novel ternary deep eutectic solvent containing ethylene glycol

Lignocellulose pretreatment often faces the problem of lignin condensation with less attention to biofuel production from total sugars, which significantly hinders biomass fractionation and energy utilization efficiency. The aim of this work was to design a novel ternary deep eutectic solvent (DES) (citric acid: choline chloride: ethylene glycol in a molar ratio of 1:1:2) for pretreatment of wheat straw with a solid/liquid ratio (S/L) of 1:20 (w/w) to achieve non-condensed lignin and high-yield fermentable sugars at 100 °C for 12 h. The introduction of ethylene glycol into ternary DES could capture the formed benzyl carbocation to protect lignin structure, inhibit excessive degradation of hemicellulose but to fermentable sugars (e.g., xylose), and improve the enzymatic digestibility of cellulose (98.73%, w/w). The mechanisms of both lignin fractionation and the in-situ lignin protection during the pretreatment of wheat straw enabled by ternary DES were systematically elucidated. Importantly, Trichosporon cutaneum cultivated in undetoxified DES waste liquid (hemicellulose-rich) and residue hydrolysate (cellulose-rich) could totally afford 8.7 g microbial lipid per 100 g wheat straw, which is superior to previous reports. This green in-situ lignin protection and fractionation strategy provides a new avenue for upgrading lignocellulose to microbial lipids.

Dual-stage biorefinery to convert spentwash hydrolysate into oleochemicals using Trichosporon cutaneum and Yarrowia lipolytica

Yeast lipids from low-cost renewable feedstock are valuable resources for oleochemicals thus enabling circular chemistry. Current study focuses on lipid and volatile fatty acid (VFA) production through dual-stage fermentation of spentwash in a biorefinery framework with Trichosporon cutaneum (Tc) and Yarrowia lipolytica (Yl). During cell proliferation phase, Tc and Yl accumulated 2.9 and 2.5 g/L of dry biomass respectively in acid-hydrolysed spentwash (AHSW) and produced 16 and 5.5 g/L of total VFA respectively. Lipid yields (29.8%) and lipid titres (0.89 g/L) were higher in Tc/AHSW, when compared to Yl indicating the efficacy of Tc in spentwash bioremediation. Lipid accumulation was enhanced to 35% in Tc/AHSW, in presence of 0.05% NH4Cl due to oxidative stress of ammonium ions. Analysis of fatty acid composition revealed the presence of higher oleic acid, which is ideal for biodiesel production. The results demonstrate a sustainable biorefinery model for bioremediation of spentwash and its value addition.

Microbial Lipid Production from High Concentration of Volatile Fatty Acids via Trichosporon cutaneum for Biodiesel Preparation.

Direct bioconversion of high concentration of volatile fatty acids (VFAs) into microbial lipid is challenging due to the aggravated cytotoxicity of VFAs at high loadings. Herein, a robust oleaginous yeast Trichosporon cutaneum was screened for lipogenesis from high concentration of VFAs using a regular batch culture. Biomass and lipid content of 8.9g/L and 49.1%, respectively, were attained from 50g/L acetic acid with 90.9% of which assimilated within 10days. The blend of VFAs (50g/L), with mass ratio of acetic, propionic, and butyric acids of 6:3:1, was found superior to acetic acid for lipogenesis. Biomass and lipid titer increased by 16.9% and 18.2%, respectively, with the three VFAs completely consumed within 8days. Butyric acid was assimilated simultaneously with acetic acid at the beginning of the culture. Heptadecanoic acid (C17:0) and heptadecenoic acid (C17:1) were produced when propionic acid co-existed with acetic and butyric acids. The estimation of biodiesel properties indicated that lipid prepared from VFA blend showed superiority to acetic acid for high-quality biodiesel production. This study strongly supported that T. cutaneum permitted high concentration of VFA mixture for lipid production.

Ultra-centrifugation force in adaptive evolution changes the cell structure of oleaginous yeast Trichosporon cutaneum into a favorable space for lipid accumulation.

Microbial lipid production from lignocellulose biomass provides an essential option for sustainable and carbon-neutral supply of future aviation fuels, biodiesel, as well as various food and nutrition products. Oleaginous yeast is the major microbial cell factory but its lipid-producing performance is far below the requirements of industrial application. Here we show an ultra-centrifugation fractionation in adaptive evolution (UCF) of Trichosporon cutaneum based on the minor cell density difference. The lightest cells with the maximum intracellular lipid content were isolated by ultra-centrifugation fractionation in the long-term adaptive evolution. Significant changes occurred in the cell morphology with a fragile cell wall wrapping and enlarged intracellular space (two orders of magnitude increase in cell size). Complete and coordinate assimilations of all nonglucose sugars derived from lignocellulose were triggered and fluxed into lipid synthesis. Genome mutations and significant transcriptional regulations of the genes responsible for cell structure were identified and experimentally confirmed. The obtained T. cutaneum MP11 cells achieved a high lipid production of wheat straw, approximately five-fold greater than that of the parental cells. The study provided an effective method for screening the high lipid-containing oleaginous yeast cells as well as the intracellular products accumulating cells in general.

Complete Genome Sequencing and Comparative Analysis of the Clinically-Derived Apiotrichum mycotoxinivorans Strain GMU1709.

Over the past decade, Apiotrichum mycotoxinivorans has been recognized globally as a source of opportunistic infections. It is a yeast-like fungus, and its association as an uncommon pulmonary pathogen with cystic fibrosis patients has been previously reported. Immunocompromised patients are at the highest risk of A. mycotoxinivorans infections. Therefore, to investigate the genetic basis for the pathogenicity of A. mycotoxinivorans, we performed whole-genome sequencing and comparative genomic analysis of A. mycotoxinivorans GMU1709 that was isolated from sputum specimens of a pneumonia patient receiving cardiac repair surgery. The assembly of Oxford Nanopore reads from the GMU1709 strain and its subsequent correction using Illumina paired-end reads yielded a high-quality complete genome with a genome size of 30.5 Mb in length, which comprised six chromosomes and one mitochondrion. Subsequently, 8,066 protein-coding genes were predicted based on multiple pieces of evidence, including transcriptomes. Phylogenomic analysis indicated that A. mycotoxinivorans exhibited the closest evolutionary affinity to A. veenhuisii, and both the A. mycotoxinivorans strains and the formerly Trichosporon cutaneum ACCC 20271 strain occupied the same phylogenetic position. Further comparative analysis supported that the ACCC 20271 strain belonged to A. mycotoxinivorans. Comparisons of three A. mycotoxinivorans strains indicated that the differences between clinical and non-clinical strains in pathogenicity and drug resistance may be little or none. Based on the comparisons with strains of other species in the Trichosporonaceae family, we identified potential key genetic factors associated with A. mycotoxinivorans infection or pathogenicity. In addition, we also deduced that A. mycotoxinivorans had great potential to inactivate some antibiotics (e.g., tetracycline), which may affect the efficacy of these drugs in co-infection. In general, our analyses provide a better understanding of the classification and phylogeny of the Trichosporonaceae family, uncover the underlying genetic basis of A. mycotoxinivorans infections and associated drug resistance, and provide clues into potential targets for further research and the therapeutic intervention of infections.

MICROBIOLOGICAL AND TREATMENT STUDIES OF OVINE FOOTROT INFECTION IN MOSUL AREA

It emerges from bacteriological study that it is possible to isolate many bacterial types and yeasts from the lesions of footrot infection in sheep. Anaerobic cultivation came out with the isolation of Spherophorus spp. (24%), Bacteroides spp. (60%), Corynebacterium spp. (64%), Enterobacteracae (76%), Streptococcus spp. (76%), Staphylococcus spp. (36%), Clostridum sordellii (46%) and Irichosporon cutaneum (4%). 
 On the other hand aerobic cultivation rendered the isolation of Corynebacterium spp. (100%), Entrobacteracae (100%), Staphylococcus spp. (15%), Penicillium spp., Aspergillus fumigatus and Trichosporon cutaneum. 
 Five different drug combinations were studied for their efficacy in the treatment of ovine footrot. Their healing rates were as follows : oxytetracycline (59%), oxytetracyclin with formaline (70.9%), Procaine penicillin and streptomycin (72.5%), Procaine penicillin and streptomycin with formaline dipping (80.76%), Formaline alone (63.8%). All kinds of treatment indicated statistically significant differences to exit between the treated (experimental) and untreated (control) groups

Rhamnolipids as a Tool for Eradication of Trichosporon cutaneum Biofilm.

Microbial biofilms formed by pathogenic and antibiotic-resistant microorganisms represent a serious threat for public health in medicine and many industrial branches. Biofilms are involved in many persistent and chronic infections, the biofouling of water and food contamination. Therefore, current research is involved in the development of new treatment strategies. Biofilm is a complex system, and thus all aspects of the measurement and monitoring of its growth and eradication in various conditions, including static and dynamic flow, are issues of great importance. The antibiofilm character of rhamnolipid mixtures produced by four Pseudomonas aeruginosa strains was studied under different conditions. For this purpose, the biofilm of opportunistic pathogen Trichosporon cutaneum was used and treated under static conditions (microscope glass coverslip in a Petri dish) and under dynamic conditions (a single-channel flow cell). The results show that the biological activity of rhamnolipids depends both on their properties and on the conditions of the biofilm formation. Therefore, this aspect must be taken into account when planning the experimental or application design.

Tolerance of Trichosporon cutaneum to lignin derived phenolic aldehydes facilitate the cell growth and cellulosic lipid accumulation

Phenolic aldehydes are the major inhibitors from lignocellulose pretreatment. Previous studies show that oleaginous yeasts are difficult to survive in lignocellulosic hydrolysates even after the removal of furan aldehydes and organic acids inhibitors. This study investigated the cell viability, sugar consumption and lipid accumulation of the major oleaginous yeasts including Trichosporon cutaneum, Rhodosporidium toruloides, Rhodotorula glutinis, Yarrowia lipolytica in wheat straw hydrolysate containing only phenolic aldehydes after furan aldehydes and organic acids were selectively degraded by microorganisms. The results confirmed that the existence of residual phenolic aldehydes was the major reason for poor cell growth and metabolism of oleaginous yeasts. Only T. cutaneum demonstrated the higher tolerance by biodegrading phenolic aldehydes and the satisfactory cell growth and lipid production were obtained. This study revealed that T. cutaneum might be one of the promising cell factories for microbial lipid production from lignocellulosic feedstock.

Microbial lipid fermentation of Trichosporon cutaneum in high saline water

Fermentative production of microbial lipid requires high fresh water input. The utilization of high saline seawater or industrial wastewater is an important alternative to reduce the freshwater consumption. This study revealed that oleaginous yeast Trichosporon cutaneum was tolerant to a high salinity up to 130 g/L of NaCl after long-term adaptive evolution. Lipid fermentation of T. cutaneum in seawater achieved the lipid production of 31.7 g/L with approximately 36% greater than that in freshwater. The saline water containing phenol was also tested for lipid fermentation and 23.6 g/L of lipid was produced simultaneously with the complete biodegradation of phenol. An interesting phenomenon was also observed that the yeast cells spontaneously segregated onto the upper surface of the saline water. This study extended the lipid fermentation options with practical application potentials.

Comparative study of lipid production from cellulosic ethanol fermentation wastewaters by four oleaginous yeasts

The biochemical treatment of cellulosic ethanol wastewater (CEW) is considered to be an ideal green process. To screen out the best oleaginous yeastfor the utilization of cellulosic ethanol wastewater, four oleaginous yeasts (Trichosporon cutaneum, Rhorosporidium toruloides, Cryptococcus albidus and T. coremiiforme) were compared to assess their abilities for lipid production in terms of biomass production, lipid content and lipid yield. Furthermore, thechemical oxygen demand (COD) conversion rate, COD degradation and fatty acid composition were calculated to analyze the effect of wastewaters treatment. According to the fermentation results, the highest biomass and lipid yield of T. cutaneum in CEW were 20.945 and 1.56 g/L, respectively, while the R. toruloides reached the highest lipid content (17.32%). The maximum conversion rates of T. cutaneum are 73.64 and 6.06%, respectively, in terms of conversion yield of biomass/COD and lipids/COD. The content of fatty acids showed that after six days’ fermentation, T. coremiiforme obtained the highest unsaturated fatty acid content, the content of C18:1 and C18:2 was 57.64%. This study suggests that T. cutaneum has great potential for lipid production and wastewaters treatment from cellulosic ethanol fermentation.

Microwave-assisted pretreatment of harmful algal blooms for microbial oil-centered biorefinery approach

Utilization of harmful algal blooms (HABs) for the cultivation of oleaginous microorganisms can provide dual benefits of mitigating the toxicity from the aquatic reservoirs and generation of copious media for biodiesel production. In the present investigation, microwave-assisted dilute alkali-freeze pretreatment was optimized to develop a low-cost growth medium from HAB dried biomass. The electron micrographs along with the elemental analysis confirmed the efficient breakage of HABs after the microwave-assisted hydrolysis treatment as compared with the acid hydrolysis. Moreover, the sugar analysis revealed ~ 46% higher carbohydrate content in microwave-assisted hydrolysate as compared with acid hydrolysate. The microwave-assisted hydrolysate and conventional dilute acid hydrolysate were then used to cultivate microalga (Chlorella minutissima) and yeast (Trichosporon cutaneum) for biomass and lipid accumulation and compared to artificial media. Microalga showed ~ 1.3- and 2-fold higher dry cell weight (DCW) and lipid content, respectively, while the yeast growth increased by ~ 27% with lipid content of 30%. The fatty acid profiles and biodiesel properties were also amenable to the international biodiesel standards. Hence, the present study provides a proof-of-concept of utilizing HAB hydrolysate for culturing oleaginous microorganisms for potential biodiesel production.