Two-spored basidia occur in a number of Basidiomycetes, among which may be mentioned the common cultivated form of Agaricus campestris, Amanita bisporigera, Craterellus cornucopioides and a number of Gasteromycetes. In all of these cases, however, the two-spored condition is exceptional, the great majority of the species of the Agaricales having four-spored basidia; the Gasteromycetes, while more variable in this respect, frequently have more than four. The Dacryomycetaceae is the only extensive group in which the basidium is consistently two-spored. It follows that members of this family afford particularly favorable material for studies which attempt to correlate the two-spored with the more usual four-spored condition. The questions to be considered are: Does nuclear fusion take place in the young basidium, and is it followed by meiosis in the usual manner? If so, what disposition is made of the resulting four nuclei? Do two nuclei pass into each spore? Does the basidium produce a second crop of spores? Or do two of the nuclei remain within the basidium, degenerating with it? Previous reports are far from agreeing in their answers to these questions and it seems desirable to attempt to throw more light upon the problem. The species chosen, Guepinia Spathularia, was selected because it is exceedingly common in North America, it is a typical member of the Dacryomycetaceae and it has not previously been studied cytologically. Although apparently not indigenous to Europe, its taxonomic position has been considered so frequently that it may be said to be universally known. In addition, an abundant supply of early stages was at hand, permitting observations upon the morphological development of the basidiocarp, to whichl little attention has been paid in tremellaceous fungi. The reasons for
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