Pleurotus florida is a widely consumed and easily cultivated mushroom, valued for its high nutritional content. This study aimed to enhance its mineral content through substrate fortification, specifically using magnesium sulfate, to create more nutrient-rich food products. Since minerals are crucial for mushroom growth, supplementing substrates can significantly improve both incubation and fructification, addressing the need for higher-quality mushrooms in functional foods and dietary supplements. Therefore, an experiment was conducted at the Mushroom Technology Laboratory, School of Studies in Biotechnology, Pt Ravishankar Shukla University, Raipur, India, to examine the effects of Magnesium sulfate (MgSO4) on P. florida during the rainy season (July–October 2022) in both submerged and solid-state cultivation. Different concentration of MgSO4 (Control, 200 mg/kg, 400 mg/kg, 600 mg/kg, 800 mg/kg and 1000 mg/kg) was supplemented to PDA media and paddy straw for cultivation of P.floridamushroom. The study assessed fruiting body production, mycelial biomass yield, protein content, and antioxidant activity. Results showed that MgSO4 significantly increased biomass production (28.67mg/100ml and 89.06g/500g of substrate for T3 for both mycelia and fruiting body), antioxidant activity (88.90 and 96.86% for control), and bioactive content (protein 20.28and 20.72 mg/100g for both mycelia and fruiting body respectively at treatment T4), notably affecting the mushrooms' metabolic profile (p less than 0.05). These findings suggest that mineral-fortified P. florida mushrooms are rich in essential nutrients and have potential as valuable pharmaceutical products.
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