Experimental Treatments Research Articles

In Vitro Gene Therapy Using Human iPS-Derived Mesoangioblast-Like Cells (HIDEMs) Combined with Microdystrophin (μDys) Expression as the New Strategy for Duchenne Muscular Dystrophy (DMD) Experimental Treatment

Duchenne Muscular Dystrophy (DMD) is a genetic disorder characterized by disruptions in the dystrophin gene. This study aims to investigate potential a therapeutic approach using genetically modified human iPS-derived mesoangioblast-like cells (HIDEMs) in mdx mouse model. This study utilizes patient-specific myoblasts reprogrammed to human induced pluripotent stem cells (iPSCs) and then differentiated into HIDEMs. Lentiviral vectors carrying microdystrophin sequences have been employed to deliver the genetic construct to express a shortened, functional dystrophin protein in HIDEMs. The study indicated significant changes within redox potential between healthy and pathological HIDEM cells derived from DMD patients studied by catalase and superoxide dismutase activities. Microdystrophin expressing HIDEMs also improved expression of genes involved in STARS (striated muscle activator of Rho signaling) pathway albeit in selective DMD patients (with mild phenotype). Although in vivo observations did not bring progress in the mobility of mdx mice with HIDEMs, microdystrophin interventions this may argue against “treadmill test” as suitable for assessment of mdx mice recovery. Low-level signaling of the Rho pathway and inflammation-related factors in DMD myogenic cells can also contribute to the lack of success in a functional study. Overall, this research contributes to the understanding of DMD pathogenesis and provides insights into potential novel therapeutic strategy, highlighting the importance of personalized gene therapy.

Bayesian Safety and Futility Monitoring in Phase II Trials Using One Utility-Based Rule.

For phase II clinical trials that determine the acceptability of an experimental treatment based on ordinal toxicity and ordinal response, most monitoring methods require each ordinal outcome to be dichotomized using a selected cut-point. This allows two early stopping rules to be constructed that compare marginal probabilities of toxicity and response to respective upper and lower limits. Important problems with this approach are loss of information due to dichotomization, dependence of treatment acceptability decisions on precisely how each ordinal variable is dichotomized, and ignoring association between the two outcomes. To address these problems, we propose a new Bayesian method, which we call U-Bayes, that exploits elicited numerical utilities of the joint ordinal outcomes to construct one early stopping rule that compares the mean utility to a lower limit. U-Bayes avoids the problems noted above by using the entire joint distribution of the ordinal outcomes, and not dichotomizing the outcomes. A step-by-step algorithm is provided for constructing a U-Bayes rule based on elicited utilities and elicited limits on marginal outcome probabilities. A simulation study shows that U-Bayes greatly improves the probability of determining treatment acceptability compared to conventional designs that use two monitoring rules based on marginal probabilities.

Therapeutic and Safety Promise of Mesenchymal Stem Cells for Liver Failure: From Preclinical Experiment to Clinical Application.

Liver failure (LF) is serious liver damage caused by multiple factors, resulting in severe impairment or decompensation of liver synthesis, detoxification, metabolism, and biotransformation. The general prognosis of LF is poor with high mortality in non-transplant patients. The clinical treatments for LF are mainly internal medicine comprehensive care, artificial liver support system, and liver transplantation. However, none of the above treatment strategies can solve the problems of all liver failure patients and has its own limitations. Mesenchymal stem cells (MSCs) are a kind of stem cells with multidirectional differentiation potential and paracrine function, which play an important role in immune regulation and tissue regeneration. In recent years, MSCs have shown multiple advantages in the treatment of LF in pre-clinical experiments and clinical trials. In this work, we reviewed the biological characteristics of MSCs, the possible molecular mechanisms of MSCs in the treatment of liver failure, animal experiments, and clinical application, and also discussed the existing problems of MSCs in the treatment of liver failure.

Study on combination of surfactant and acid for depressure and increasing injection oil displacement in heterogeneous sandstone reservoirs

This study addresses the challenges faced by unconventional tight sandstone reservoirs, including low porosity, permeability, high clay content, and complex wettability, which lead to increased flow resistance and injection pressures. The research aims to optimize depressure and increasing injection methods by investigating the effects of various two-phase and three-phase displacement systems, employing experimental treatments including acids, alkalis, and surfactants. Nuclear magnetic resonance, computed tomography, scanning electron microscopy, inductively coupled plasma, and wettability tests are utilized to investigate the mechanisms of these treatments. Key findings indicate that weak alkaline ethylenediaminetetraacetate tetrasodium and weak acids like hydroxyethylidene diphosphonic acid and acetic acid can cause significant pore blockage, while hydrochloric acid can dissolve pore minerals, achieves a high depressure rate of 89.42%. Although surfactants exhibit a negative effect in two-phase displacement systems, they demonstrate considerable potential in three-phase displacement. Surfactants can modify the wettability of rock surfaces, reduce oil saturation, and improve water phase permeability, resulting in a depressure rate of 11.68%. Notably, the combination of surfactants and HCl enhances the depressure rate to 60.82% and improves oil displacement efficiency from 26.12% to 57.96%. The optimal formulation identified is “0.5% unconventional agent (CNI-A) +3% HCl,” which improves oil displacement capacity and alleviates injection pressure, providing valuable insights for the management of heterogeneous sandstone reservoirs.

Using pack animals instead of tractors in Central Italy's protected areas: No evidence of reduced soil disturbance

In thinning operations in Mediterranean beech forests, pack animals are often used when topographic conditions do not allow for the use of mechanisation to perform logging operations. Otherwise, mechanised extraction is the primary choice. In an effort to reduce soil disturbance caused by mechanised ground-based logging, the forest authorities who guard protected areas have been mandating animal logging at all logging sites in recent years. Since no comparative scientific trials of soil disturbance of these two extraction systems have ever been conducted in the target setting, there is no scientific evidence to sustain this approach. On this basis, we chose two study areas where the forest authorities mandated animal logging. Each study area consists of three sub-compartments, one unharvested, one harvested by animals, and one harvested by tractor in the same year (2021). We examined soil compaction, soil organic matter, and soil microarthropod biodiversity in animal skid trails, tractor skid trails, soil unaffected by extraction methods, and unharvested control sites in each study areas. We used linear mixed-effects models, indicating the study area as random intercept, to investigate the effect of the experimental treatments on the target soil features, and to check the relationship among soil physico-chemical parameters and soil microarthropod biodiversity. We discovered that only the animal and tractor skid trails showed signs of disturbance in terms of soil compaction and organic matter loss. Additionally, there was a significant decline in the biodiversity of soil microarthropods in both the skid trail types, with values of the QBS-ar index which were practically halved in comparison to the control area. We did not find any significant difference in the values in the tractor and animal skid trails for any of the examined soil characteristics. Low soil microarthropod biodiversity was found to be correlated with high soil compaction in terms of penetration resistance and low soil organic matter levels, according to linear mixed-effects models. Despite our preliminary findings, we made it clear that the short wood system by a tractor fitted with forwarding bins is unquestionably a very soil-disturbing extraction system, that ought to be avoided when conducting logging operations in protected areas. However, pack animals are not a suitable solution to reduce soil disturbance. Other extraction systems such as chute, small-scale cable yarders, as well as combination of animals and machine avoiding extensive traffic on the forest soil, are better solutions to the fundamental issue of low-impact logging in protected areas.

The impact of apple cider vinegar on nonalcoholic fatty liver disease in rainbow trout, Oncorhynchus mykiss: A study of therapeutic potential and health benefits

AbstractIn this study, we aimed to experimentally induce fatty liver disease in rainbow trout, Oncorhynchus mykiss, and then assessed the illness recovery process, growth, and changes in the expression of FAAH and ACADL genes in both healthy (0 [C2] and 4% apple cider vinegar [T4]) and diseased fish (0 [C1], 1 [T1], 2 [T2], and 4% [T3]) apple cider vinegar. To conduct the study, 180 rainbow trout were randomly assigned to six different experimental treatments, each with three replications. The investigation lasted for 60 days. Growth indices, liver histology, blood biochemical parameters, and transcription of the ACADL and FAAH genes in the liver tissue were measured. The study found no significant differences in the final weights across all the treatments. Apple cider vinegar (ACV) administration resulted in a decrease in AST, ALT, and ALP; however, these values did not show a significant difference from C2. In T3, triglycerides significantly decreased (p < 0.05), whereas in T4, triglycerides significantly increased (p < 0.05). Hepatocytes from ACV‐containing treatments showed reduced fat compared with T4 and the control group (C1). While there was a significant difference (p < 0.05) in the expression of the FAAH gene, there was no significant difference (p > 0.05) in the expression of the ACADL gene between experimental treatments. The findings of our study indicate that an inclusion of up to 2% ACV may have positive effects on trout aquaculture and NAFLD treatment.

Chitosan-based NPK nanostructure for reducing synthetic NPK fertilizers and improving rice productivity and nutritional indices

Researchers have repeatedly emphasized how urgently we have to decrease the massive nitrogen fertilizer consumption to support agricultural productivity and maintain a sustainable ecosystem. Using chitosan (CS) as a carrier for slow release is considered a potential tool for reducing synthetic fertilizer and improving crop productivity. Therefore, two field experiments were arranged in a randomized complete block design to investigate the effects of seven treatments including synthetic fertilizer and exogenous application of chitosan-based NPK nano-structure (Ch/NPs-NPK) on growth, productivity, and nutrient uptake traits of rice as a worldwide strategy crop during 2022 and 2023 growing seasons. The experimental treatments were: T1 = full recommended synthetic NPK (recommended urea, superphosphate, potassium sulfate; control treatment), T2 = 70% of T1 + Ch/NPs-NPK 100 ppm, T3 = 70% of T1+ Ch/NPs-NPK 200 ppm, T4 = 70% of T1+ Ch/NPs-NPK 300 ppm, T5 = 30% of T1+ Ch/NPs-NPK 100 ppm, T6 = 30% of T1+ Ch/NPs-NPK 200 ppm, and T7 = 30% of T1 + Ch/NPs-NPK 300 ppm. The results revealed that T4 (i.e., 70% of recommended NPK+ Ch/NPs-NPK 300 ppm) and T1 (full recommended synthetic NPK) resulted in the highest and most significant growth and yield traits of rice as well as nutrient grain contents compared to other treatments. Therefore, combining 70% of recommended NPK with Ch/NPs-NPK 300 ppm as an exogenous application can be a smart choice for reducing synthetic NPK fertilizers by 30% in paddy fields without producing a significant decline in terms of growth, yield characteristics, or nutrient grain contents when applying the full recommended synthetic NPK.

The impact of 12 weeks of circuit body weight training on athletes’ physical fitness

The purpose of this study is to find out the impact of 12-week circuit body weight training on athletes’ physical fitness. This study used an experimental design with a control group. This experimental model consists of three stages. Firstly, pre-testing the dependent variable before implementing the treatment. Secondly, experimental participants are given experimental treatment, specifically the circuit body weight approach. Thirdly, provide a post-test to measure the dependent variable after treatment. The experimental sample included 25 athletes from various sports who were separated into two treatment groups. The circuit body weight group consisted of 13 samples, while the remaining 12 were trained using conventional direct teaching methods. The data analysis technique begins with calculating the data’s normality. The homogeneity test determines if two data sets contain homogeneous versions. Finally, the Mann-Whitney test compares two similar population means. Calculations using SPSS IBM 26 on data before treatment in the experimental class resulted in a valid sample size of 25 and a score of 48.40 ± 2,931. The experimental group had a valid sample size of 25, with a score of 49.57 ± 2,979. Pre-test data for the control class showed 25 valid samples with a score of 38.7 ± 6,607. Meanwhile, the control class post-test data obtained a valid sample size of 25 with a score 39.43 ± 6.796. The table shows a sig (2-tailed) value of 0.000 < 0.05, indicating a significant difference in physical fitness scores between the experimental and control groups. Additionally, there is a difference in average Physical Fitness results between the Circuit bodyweight training model and the conventional learning model.

디지털기기를 활용한 가정연계 자연체험활동이 유아의 환경보전태도 및 창의성에 미치는 영향

The purpose of this study is to verify the effects of home-linked nature experiences using digital devices on the environmental conservation attitudes and creativity of 3-year-old children. A total of 26 randomly selected children from an A workplace daycare center in Gyeonggi Province were assigned to either an experimental group (13 children from A Daycare Center) or a comparison group (13 children from B Daycare Center). The experimental treatment was conducted twice a week for 8 weeks from April 15, 2024, to June 7, 2024. The experimental group participated in home-linked nature-based activities using digital devices, while the comparison group engaged in thematic activities from the Nuri curriculum. Data were analyzed using t-tests (SPSS/WIN 25.0) to compare the pre- and post-scores of the experimental and comparison groups. The results were as follows: First, the experimental group showed significant improvements in environmental conservation attitudes and all sub-variables compared to the comparison group. Second, the experimental group also showed significant gains in creativity compared to the comparison group. This study is meaningful in that it verifies the positive effects of home-linked nature-based activities using digital devices on fostering environmental conservation attitudes and creativity, offering implications for early childhood education.

텃밭활동이 만 4세 유아의 편식, 식습관, 환경 친화적 태도에 미치는 효과

Objectives The purpose of this study was to verify the effects of garden activities on the eating habits, picky eating, and environmentally friendly attitudes of 4-year-old children. Methods Twelve children from A Daycare Center located in Gyeonggi Province were randomly assigned to the experimental group, and 12 children from B Daycare Center were randomly assigned to the comparison group. The experimental treatment was conducted three times a week for 13 weeks from April 15, 2024 to July 12, 2024.The experimental group experienced garden activities and conducted theme activities. Data processing was performed by comparing and analyzing the pre- and post-scores of the experimental and comparison groups using t-tests and using SPSSWIN 25.0. Results First, the experimental group that experienced gardening showed significant differences from the comparison group in both eating habits and sub-variables. Second, the experimental group that experienced gardening showed significant differences from the comparison group in all picky eating. Third, the experimental group that experienced gardening showed significant differences from the comparison group in both environmentally friendly attitudes and sub-variables. Conclusions It is significant that the effects of garden activities on children's eating habits, picky eating, and environmentally friendly attitudes have been verified, and it suggests that it can be applied to actual early childhood education settings to foster children's eating habits, picky eating, and environmentally friendly attitudes.

First principles investigation of elastic, electronic and thermoelectric properties of lead-free Cs–X–I (X = Pb, Gd, Nd, Y) perovskites

Abstract Perovskites have become the center of recent research for their possible application in perovskite solar cells, owing to their desirable optical and electronic properties, flexibility, tunability, and low–cost fabrication. Most of the perovskites are however made of lead, which is a highly poisonous element. It is therefore necessary to seek alternative perovskites for this application that are less toxic. This study investigated the elastic, electronic, and thermoelectric properties of Cs–X–I (X = Pb, Gd, Nd, and Y) as possible replacements to the leaded CsPbI3 due to their less toxic nature. The density functional theory was utilized in the computations, with quantum espresso and BoltzTrap packages. The results showed that all the materials were structurally stable. The computed mechanical properties also showed that all the other materials had better elastic constants compared to those of CsPbI3. CsPbI3 was observed to exhibit the lowest band gap, unlike the others. Moreover, the other materials possessed higher elastic constants, electrical conductivities, and lowest thermal conductivities, which are highly needed in the perovskite solar cells. However, an experimental treatment needs to be done on the studied structures in order to confirm the properties obtained in this work.

Allelopathic interactions of Carthamus oxyacantha, Macrophomina phaseolina and maize: Implications for the use of Carthamus oxyacantha as a natural disease management strategy in maize.

Fungicides are used to control phytopathogens but all these fungicides have deleterious effects. Allelopathic interactions can be harnessed as a natural way to control the pathogens but there are no reports that show the allelopathic interactions of donor plant, recipient crop, as well as the target plant pathogen and the material used for inoculum production. So, in the present study, the suitability of Carthamus oxyacantha M. Bieb. was assessed against Macrophomina phaseolina, the cause of charcoal rot in maize. Among the various treatments in pot experiment, a negative control, 3 concentrations of inoculum (1.2×105, 2.4×105, and 3.6×105 colony forming units (CFU) mL-1, 3 concentrations (0.5, 1.0, and 1.5% w/w) of C. oxyacantha along with an autoclaved M. phaseolina (Mp) and C. oxyacantha alone were included to investigate their allelopathic effects on maize, not investigated earlier. Maximum suppression of the disease was observed by 1.5% (w/w) concentration of C. oxyacantha. Soil amendment with C. oxyacantha significantly suppressed the disease incidence (DI) and disease severity index (DSI) in charcoal rot of maize up to 40 and 55%, respectively over the strongest level of inoculum (Mp3). C. oxyacantha not only reduced area under disease incidence progress curve (AUDIPC) and area under disease severity progress curve (AUDSPC), but also improved the morphological, biochemical and physiological parameters of maize. The maximum increase of 48, 65, and 75% in values of shoot length (SL), shoot dry mass (SDM), and root dry mass (RDM), respectively was observed by application of the highest concentration of C. oxyacantha in the treatment Mp1+Co3, over infested control (Mp1). Photosynthetic pigments, such as chlorophyll a, chlorophyll b and carotenoids were increased to 58, 64, and 46%, respectively over Mp1, by the application of C. oxyacantha. Carbon assimilation rate (A), stomatal conductance (gs), rate of transpiration (E), and internal carbon dioxide concentration (Ci) were significantly increased to 58, 48, 48, and 20%, respectively over infested control (Mp3), by application of C. oxyacantha concentration 1.5 (w/w). Moreover, defense enzymes like superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities were boosted up to 27, 28, and 28% over Mp3, respectively. Positive allelopathy of C. oxyacantha towards maize and negative allelopathy towards M. phaseolina makes C. oxyacantha a suitable candidate for charcoal rot disease control in maize.

Investigation of Phytochemical and Antidepressants Activity of Cinnamon Powder Extract

Neurodegenerative disease is the most common type of mobility issue, but unfortunately, there is now no medication that can alter the course of the disease. We don't know what causes this ailment. In mouse models of Parkinson's disease induced with 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine, the oral administration of cinnamon powder and sodium benzoate may prevent the death of dopaminergic cells, dysregulation of striatal neurotransmitters, and motor impairments. The mechanisms driving its function include controlling autophagy, antioxidant effects, Parkin, DJ-1, and glial cell line-derived neurotrophic factor activation, TLR/NF-κB pathway modulation, and excessive proinflammatory response prevention. Moreover, research carried out in both laboratory and living organism settings has shown that cinnamon extracts may impact the oligomerisation and aggregation of α-synuclein. This article's goal is to discuss recent findings about this phytochemical's potential as a novel treatment for Parkinson's disease (PD). We highlight additional areas of mechanism that require investigation and possible constraints that must be overcome before this phytochemical may be used in PD trials. Neurodegenerative disease is the most common type of mobility impairment, and unfortunately, there is now no medication that can alter this disease. We don't know what causes this ailment. There has been a recent uptick in interest in medicinal plant use because of the novelty, safety, and relative affordability of this field. The characteristic flavour and aroma of cinnamon, a spice that is often used, may have neuroprotective effects on people with Parkinson's disease (PD) and other neurodegenerative diseases. The essential oils of Cinnamomum species, such as cinnamaldehyde and sodium benzoate, have shown in vitro that they can protect cells from oxidative stress, ROS generation, and autophagy dysregulation. Consequently, these oils may exert a neuroprotective effect. The in vivo evidence suggests that cinnamon powder and sodium benzoate, when administered orally to Parkinson's disease models in mice, may prevent the death of dopaminergic cells, dysregulation of striatal neurotransmitters, and motor deficits. In this essay, we will go over the latest research on this phytochemical and its potential as a novel treatment for Parkinson's disease (PD). Incorporating this phytochemical into experimental PD treatments requires further investigation into additional molecular aspects and the potential overcoming of constraints and obstacles.

Liver Transplantation for Intrahepatic Cholangiocarcinoma After Chemotherapy and Radioembolization: An Intention-To-Treat Study

Liver transplantation (LT) is a potentially curative experimental treatment for unresectable intrahepatic cholangiocarcinoma (iCC). Pre-transplant downstaging may help defining tumor aggressiveness and drive patient selection. We report the preliminary results of LT for liver-limited unresectable iCC after sequential downstaging with systemic chemotherapy and radioembolization (SYS-TARE). In case of sustained disease stability after SYS-TARE, patients underwent surgical nodal sampling and, if negative, were listed for LT. In this study, 13 patients with unresectable iCC underwent downstaging with SYS-TARE. The median age was 70 years and 77% were female. All had single bulky lesions at diagnosis. After SYS-TARE, 9 (69%) dropped out: 3 due to progressive disease after TARE with no response to second-line, 4 due to extrahepatic disease development and 2 due to positive nodal disease at pre-listing abdominal exploration. The median OS after dropout was 11.5 months. Four (31%) were successfully listed and transplanted. At pathology, viable tumor ranged from 30% to less than 5%. All four patients are alive and disease-free at 73, 40, 12, and 8 months from LT. LT for unresectable iCC after downstaging with SYS-TARE appears to select suitable patients for LT, achieving optimal oncological outcomes in case of response to therapy and no lymphnodal spread.

Maternal Supplementation of Collagen Peptide Chelated Trace Elements Enhances Skeletal Muscle Development in Chicks.

Maternal nutrition plays an important role in regulating the growth and development of offspring. Collagen peptide chelated trace elements (PTE), as a new additive, have been proven to have a positive maternal effect on the intestinal health of offspring, but its effect on the growth anddevelopment is still unclear. This study aimed to investigate the effect of maternal PTE supplementation on the skeletal muscle development of offspring. A total of 270 breeder hens were randomly divided into 3 groups, and fed basal diet (CON), basal diet + 500mg/kg PTE (L-PTE), and 1000mg/kg PTE (H-PTE) for 8weeks. A total of 180 eggs were collected from each group for incubation, and then the hatched male chicks (6 replicates, 12 chicks/replicate) were allocated according to maternal treatment for a 14-day feeding experiment. The results showed that maternal PTE supplementation significantly increased the deposition of iron (Fe), zinc (Zn), and manganese (Mn) in the egg yolk (P < 0.05). In comparison with the CON group, the body weight (days 1 and 14), breast muscle weight (day 14), and muscle fiber density (day 14) of broilers were increased in the L-PTE group (P < 0.05). The serum creatinine (CREA) levels in 1-day-old broilers were reduced in the L-PTE group and H-PTE group(P < 0.05). Additionally, maternal PTE supplementation could upregulate the relative expression level of catalase (CAT) mRNA in breast muscle (P < 0.05). The relative mRNA levels of hemeoxygenase-1 (HO-1) and superoxide dismutase 1(SOD1) in the H-PTE group were significantly upregulated (P < 0.05). Moreover, PTE treatment upregulated the mRNA expression of skeletal muscle development-related genes (Pax7 and MyoG) and the IGF signaling pathway (mTOR, IGF-1R, and IGF-2R) (P < 0.05). In conclusion, maternal PTE supplementation may improve the growth performance and skeletal muscle development of offspring by activating the IGF signaling pathway.

Can AI Robot Teaching Improve Children’s Performance in Housework? A Quasi-Experimental Study

Background: The primary objective of this study is to explore the effects of using Artificial Intelligence (AI) robot-assisted instruction in preschool activities on young children’s performance in housework. Methods: A quasi-experimental design was employed, with an experimental group and two control groups, to observe changes in children’s performance in housework before and after AI robot-assisted teaching. The study sample consisted of preschoolers from metropolitan Taipei, with 65 children in the experimental group, 53 in control group 1, and 75 in control group 2. Children participating in the research project required consent from the school, teachers, and parents. The children in the experimental group received AI robot-assisted instruction on household tasks for two days a week over three consecutive weeks, with each session lasting approximately 20–30 min. Control group 1 did not receive any experimental treatment, while control group 2 underwent the same learning schedule and content as the experimental group but was instructed by teachers instead of AI robots. The data were analyzed based on pre- and post-test surveys of parents observing their children’s performance in household tasks. Results: This study found that both AI robot-assisted teaching and teacher-led instruction enhanced children’s household skills, with the AI robot-assisted method showing slightly better results. These findings suggest that both technological tools and teacher guidance can effectively improve children’s housework performance, while artificial intelligence robots may provide young children with motivation and curiosity to learn due to their appearance and interactive design, which deserves further analysis. Conclusion: As technology rapidly advances, young children are exposed to various technological devices at an early age. Integrating technological media into future preschool teaching is inevitable, and leveraging tools like AI robots to support teaching, reduce teacher burden, and diversify instructional methods could be a direction worth considering.

Nanomedicine in Cancer Treatment: Mechanisms, Applications, and Future Directions

Nanomedicine, an emerging field at the intersection of nanotechnology and medicine, holds significant promise for revolutionizing cancer treatment. By utilizing materials at the nanoscale, ranging from 1 to 100 nanometers, nanomedicine offers a versatile platform for targeting tumors, improving drug delivery, and minimizing side effects. This paper explores the design and preparation of nanoparticles, including common materials like liposomes, polymers, metals, and quantum dots, as well as their mechanisms of targeted delivery through both passive and active methods. Key applications of nanomedicine are discussed, particularly in chemotherapy, immunotherapy, and imaging. Clinical progress is highlighted through examples such as FDA-approved drugs like Onivyde for pancreatic cancer and experimental treatments like BIND-014 for metastatic prostate cancer. Despite rapid advancements, significant challenges remain, particularly in scaling up production and ensuring batch consistency. Safety concerns, such as nanoparticle toxicity and unintended accumulation in organs, must be addressed through rigorous testing and design improvements. Ethical considerations and regulatory hurdles also pose obstacles to the widespread clinical adoption of nanomedicine. Looking forward, multifunctionalization and personalized nanomedicine represent key areas of future growth, offering the potential for highly individualized treatments that combine therapeutic and diagnostic capabilities. Continued research and collaboration across scientific disciplines will be crucial in overcoming current limitations and unlocking the full potential of nanomedicine for cancer treatment.

Effect of P Reduction on phoD-Harboring Bacteria Community in Solar Greenhouse Soil

Phosphorus (P) enrichment frequently occurs in the soil used in greenhouse vegetable production (GVP). Minimizing the application of P fertilizer represents a crucial approach to mitigating the accumulation of P in the soil and enhancing its utilization efficiency. However, the changes in bacterial communities and the turnover mechanism of soil P fractions related to soil P cycling after P fertilizer reduction are still unclear. To unravel these complexities, we devised three experimental treatments: conventional nitrogen (N), P, and potassium (K) fertilizer (N1P1K1); conventional N and K fertilizer without P (N1P0K1); and no fertilizer (N0P0K0). These experiments were conducted to elucidate the effects of P reduction on cucumber plant growth, soil P fractions, and the phoD-harboring bacterial community in the P-rich greenhouse soil. The results showed that there were no significant differences between the N1P1K1 and N1P0K1 treatments in terms of plant growth, yield, and P uptake, and the values for the N0P0K0 treatment were significantly lower than those for the N1P1K1 treatment. In a state of P depletion (N0P0K0, N1P0K1), the main P sources were Resin-Pi, NaHCO3-Pi, NaHCO3-Po, and NaOH-Pi. The contents of NaOH-Po and CHCl-Po in the N1P0K1 treatment increased significantly. Without P fertilizer, alkaline phosphatase (ALP) activity, phoD gene abundance, and bacterial community diversity were significantly increased. The abundance of Ensifer in the N0P0K0 and N1P0K1 treatments was 8 and 10.58 times that in the N1P1K1 treatment, respectively. Additionally, total phosphorus (TP) and available nitrogen (AN) were key factors affecting changes in the phoD bacterial community, while Shinella, Ensifer and Bradyrhizobium were the main factors driving the change in soil P fractions, and NaHCO3-Pi and NaOH-Pi were key factors affecting crop yield. Therefore, reducing the application of P fertilizer will increases the diversity of phoD-gene-harboring bacterial communities and promote organic P mineralization, thus maintaining the optimal crop yield.

Variety of Lettuce (Lactuca sativa L.) Growth and Yield in Response to Various Organic Fertilizers in Makurdi, Nigeria

This study was conducted in 2023 at the nursery of the Teaching and Research farm of the Department of Crop production of Joseph Sarwuan Tarka University Makurdi, Benue State, Nigeria. The experimental treatments were two (2) varieties of lettuce (ice berg and Butter head) and three (3) organic manure sources (Poultry dropping, cow dung, goat manure) and a control. An experiment was set up as a 2 x 4 factorial design using a randomized complete block design (RCBD) with three replications. Data were collected from the following parameters, plant height, plant diameter, number of leaves, leaf area index (LAI), crop growth rate (CGR), roof weight, and fresh weight. All the study parameters of lettuce were significantly (P&lt;0.05) different, on both organic manure, and variety. The iceberg variety surpassed the butter head variety, with statistically significant (P&lt;0.05) differences observed in several parameters: plant height (20.32 cm), number of leaves (23.85), leaf area index (15.19 cm³), сrop growth rate (1.54 g m³ per day), chlorophyll content (23.32 mg/g), fresh weight (83.86 g), root weight (20.23 g), and yield (3.19 t/ha) Similarty, poutry droppings obtained significantly (P&lt;0.05) better results in terms of plant height (21.73 cm), number of leaves (23 92), leaf area Index (16.75 cm²), crop growth rate (3.70 g m³ per day), chlorophyll content (25.73 mg/g), fresh weight (632.02 g), root weight (22.13 g) and yield (3.75 t/ha) as compared to other organic manure sources such as cow dung, goat manure and control. In addition, a significant difference (P&lt;0.05) was observed in the interaction between lettuce varieties selection and organic fertilizers at various levels, particularly with the high level of iceberg x poultry manure

Optimization of Amaranthus production under irrigation and poultry manure application using grey relational analysis

This study was carried out to optimize Amaranthus production under varying irrigation levels and poultry manure applications using Grey Relational Analysis. The experimental design was split-plot, where irrigation treatment was the main plot, while poultry manure was the subplot treatment. The irrigation levels are FIT50, FIT75, FIT100 and FIT135 which correspond to 50% Full irrigation, 75% full irrigation, 100% full irrigation and 135% full irrigation treatments, in combination with the poultry manure application rates M0t/ha, M5t/ha, M10t/ha and M20t/ha. Sixteen experimental treatments were arranged in a 4 x 4 factorial experimental design and replicated 3 times. Sprinkler irrigation system was adopted for the study. Results showed that there was corresponding increase in the response of Amaranthus growth characteristics and yield to the combination of irrigation and poultry manure treatments. The Crop Water-use efficiency initially showed a linear corresponding increase to an average value of 473kg/ha/mm but later exhibits a diminishing return beyond FIT100 irrigation. While production cost showed continual increase to average value of $377/ha as the inputs treatment level also increased to FIT135 irrigation combined with the poultry manure M20t/ha. Grey Relational Grades (GRG) calculated were 0.854, 0.790, 0.787, 0.765, 0.740, 0.629, 0.621, 0.620, 0.546, 0.543, 0.515, 0.475, 0.458, 0.443, 0.417 and 0.403 respectively. Amaranthus cultivated under FIT100_M20t/ha treatment matched with the highest GRG value 0.854, making FIT100_M20t/ha treatment the optimum. Analysis of variance for the GRG showed that irrigation and poultry manure are highly significant input variables for Amaranthus production. The findings from this study guide farmers on the optimal combination of water and organic fertilizer to maximize Amaranthus yield and minimize resource waste, thereby enhancing sustainability in crop production.