Treatment Of Urinary Infections Research Articles

Phytochemical screening and evaluation of antiradical and antimicrobial activities of Annona senegalensis and Detarium microcarpum leaves used in Benin to treat urinary infections

The challenge of resistance to synthetic antimicrobials necessitates new alternative solutions. Medicinal plants are rich in active compounds with various medicinal properties that can be used in treatment of urinary infections. This work aims to highlight the value of Annona senegalensis and Detarium microcarpum by evaluating phytochemical and antimicrobial potentials of their leaf extracts. Qualitative and quantitative analyses were carried out using color and precipitation reactions and spectrophotometric assays. The antiradical activity was evaluated using the DPPH test, and the antimicrobial activity was assessed by the agar diffusion method. Phytochemical screening showed that both plants contain anthraquinones, catechic tannins, coumarins, flavonoids, reducing compounds, sterols and terpenes. Hydroethanolic extract of Detarium microcarpum and A. senegalensis showed IC50 inhibition percentages of 0.20 mg/mL and 0.22 mg/mL respectively. Regarding antimicrobial activity, the hydroethanolic leaf extracts of D. microcarpum and A. senegalensis were found to be bactericidal and fungicidal against the tested bacteria and molds. This extract showed more pronounced antimicrobial activity than amoxicillin and ciprofloxacin, which are synthetic antimicrobials. Diversity of secondary metabolites and notable antimicrobial activity of D. microcarpum and A. senegalensis may justify their use in traditional medicine for treating urinary infections in Benin.

Antimicrobial Potential of Syzygium aromaticium (CLOVE) Extracts on Multidrug-Resistant (MDR) Uropathogenic Bacteria Isolated from Clinical Specimens in Bauchi, Nigeria

Background: Urinary Tract Infection (UTI) is a common pathogenic inflammatory, distressing, and occasionally life-threatening condition that affects people of all ages and gender, mostly propelled by the emergence of multidrug-resistant bacteria. Cloves are used as spices in food and flavouring agent in drinks it is also used traditionally as a treatment for urinary infections.
 Aim: This study was carried out to evaluate the antimicrobial potentials of Clove extracts on multidrug-resistant (MDR) Uropathogenic bacteria.
 Design: This is a Clinical and laboratory-based study of patient with cases of UTI
 Place and Duration of study: This study was conducted in the Microbiology laboratory of Abubakar Tafawa Balewa University Teaching Hospital (ATBUTH), Bauchi, Nigeria, from January to December, 2021.
 Methodology: Two hundred and fourty five (245) clean catch midstream urine samples were collected from patients with suspected cases of urinary tract infection attending GOPD ATBU TH. Bacteria were isolated using standard techniques and antibiotic resistant pattern was tested by Kirby Bauer Disk Diffusion method. Bioactive components of clove was extracted using diethyl ether, ethanolic and water as solvents. Phytochemical analysis of the extracts was also carried out.
 Results: Out of the samples analysed, 168 (68.6%) showed significant bacteriuria. UTI was more prevalent in women within the active age group 21-30. The isolates resistant to seven and above commonly used antibiotics are selected from each specie to test for efficacy. The extracts revealed the presence of alkaloids, glycosides, saponins, tannins, flavonoids, sterols and Triterpenes. In vitro antimicrobial activity of diethyl ether, ethanolic and aqueous extract of cloves at different concentration of: 200mg/ml, 100mg/ml, 50mg/ml, 25mg/ml, 12.5mg/ml
 and 6.25mg/ml were tested against multidrug resistant isolates. S. aureus and Klebsiella spp are the most sensitive to all clove extracts while E. coli and P. aeruginosa are less sensitive. All Three extracts showed a broad spectrum of activities at higher concentrations (200mg/ml) while no or less activity at the lower concentration of the extracts. Diethyl ether extract exerts higher activity than ethanolic and aqueous extract as revealed by the mean diameter of zone of inhibitions, MIC and, MBC values. The MIC values of the extracts were lower than their MBC values suggesting that the extracts inhibited the growth of MDR isolates while being bactericidal at higher concentrations.
 Conclusion: Staphylococcus aureus and E. coli are among the commonest uropathogens clinically encountered in this area and most of the species are resistant to commonly administered antibiotics. Clove extracts had great antimicrobial potential against these bacteria, therefore it can be used in the treatment of UTIs. However, it is necessary to determine its toxicity, pharmacokinetic properties and side effects.

A Comparative Study of Urinary Tests and Cultures for the Effectiveness of Fosfomycin in Catheter-Related Urinary Tract Infections

As the elderly population increases due to an aging society, the number of patients with catheters is increasing, and treatment for urinary infections is needed. The current study analyzed the effectiveness of fosfomycin, the primary antibiotic used to treat urinary tract infections (UTIs), in these patients. Patients who received fosfomycin as the primary antibiotic for a UTI were selected, and the results of urine tests and cultures before and after fosfomycin administration were compared and analyzed. The degree of UTI in patients with a catheter was found to be more severe (p = 0.020), and the infecting strains were found to be different depending on whether a catheter was present (p = 0.014). There was a difference in the treatment success rate depending on whether or not a catheter was present (53.6% vs. 70.4%), but it was found that the treatment rate was more than 50% regardless of whether a catheter was present. The bacterial type, as well as the treatment rate based on the bacterium, differed depending on the presence of a catheter. Fosfomycin has a success rate of more than 50%, even in patients with catheters; therefore, it can be considered the primary antibiotic for treating UTIs.

Clinical practice guideline for the diagnosis and management of urinary tract infections: 2022 update

In 2015, the "New recommendations regarding the current controversies in urinary infection" were published in the Archivos Argentinos de Pediatría. Given the fact that in these past years, new evidence has emerged regarding the diagnosis and treatment of urinary infection, the Pediatric Nephrology Committee of Sociedad Argentina de Pediatría has decided to update these recommendations. The main goal is to provide the pediatrician with the necessary tools to make a correct diagnosis, define the most appropriate treatment, select the patients who will benefit from antibiotic prophylaxis, and decide which imaging studies will be necessary, avoiding costly and invasive interventions. These guidelines also include the management of children with urinary tract infections associated with special situations such as: bladder bowel dysfunction, the newborn, children with neurogenic bladder, kidney transplant patients and fungal urinary tract infections.

Применение препаратов группы оксазолидинонов в терапии инфекции мочевыводящих путей

Рассмотрена клиническая эффективность применения оксазолидинонов с акцентом на линезолид при инфекциях мочевой системы. Отмечена высокая активность препарата при метициллин-резистентном золотистом стафилококке, Enterococcus faecium и Enterococcus faecalis, резистентных к ванкомицину. Определены преимущественные ситуации для эффективного использования линезолида: септические состояния и длительное носительство вышеуказанных патогенов.

Urinarne infekcije - savremeni pristup dijagnostici i lečenju

Urinary tract infections are among the most common infections in the human population. Inadequate interpretation of laboratory findings, as well as mistakes in the application of therapy, often lead to prolongation of treatment and recurrence of infections as well as in a bacterial resistance. The analysis and synthesis of relevant information on up-to-date recommendations in the diagnosis and treatment of urinary infections have been done by searching databases of PubMed, Google academic, Medline and the guidelines of the European Association of Urology, an analysis and synthesis of relevant information on up-to-date recommendations in the diagnosis and treatment of urinary infections will be done. Relevant guidelines recommend usage of Fosfomycin, Nitrofurantoin or Trimethoprim Sulfomethoxazole as the first line of therapy for uncomplicated cystitis in women, while the use of antibiotics in asymptomatic bacteriuria is reserved only for immunocompromised conditions, pregnancy, in patients with chronic recurrent infections and in patients undergoing endourological procedures. Following the modern guidelines of the European Association of Urologists on the treatment of urinary infections enables the most adequate treatment of patients.

Antibiotska rezistencija uzročnika infekcija urinarnog trakta kod pacijenata Instituta za javno zdravlje Kragujevac

Introduction/Aim: Urinary tract infections are one of the most common infections in primary healthcare. The aim of this study was the microbiological analysis of urine of infirmary patients at the Institute of Public Health in Kragujevac during the six-month period (1 st November 2020 -30th April 2021). Methods: Causes of urinary tract infections were identified retrospectively, according to the collected data, and their sensitivity to antibiotics was determined with the help of antibiogram method. Results: A six-month analysis of urine samples of infirmary patients included 4809 urine samples. Of all analyzed samples, 1218 (25.3%) were positive to pathogens. The commonest urinary tract pathogens in the examined period were the following: Escherichia coli 842 (69.1%), Klebsiella spp. 252 (20.7%), Pseudomonas aeruginosa 71 (5.8%) and Enterococcus group 53 (4.4%). E. coli was more frequent in women (82.7%), while Pseudomonas aeruginosa was more frequent in men (76.1%). Klebsiella spp. and Enterococcus spp. were almost equally present in men and women. First-choice drugs in the treatment of urinary infections would be the following: for E. coli amikacin (aminoglycoside) and fosfomycin, and for Klebsiella spp. meropenem (carbapenem) and also amikacin. As far as P. aeruginosa is concerned, meropenem was shown to be the most efficient, as well as the penicillin antibiotic piperacillin-tazobactam. Conclusion: Considering that uropathogens possess mechanisms for the development of resistance, the treatment should include antibiotics depending on the results of antibiogram.

Выбор антибактериального препарата при инфекциях мочевыделительной системы у детей

Инфекции мочевыделительной системы в детском возрасте занимают второе место по частоте обращений за первичной медицинской помощью. Escherichia coli — наибольший причинно-значимый возбудитель внебольничных форм инфекций мочевыделительной системы, которые, как правило, носят неосложненный характер и чаще диагностируются у девочек после трехмесячного возраста. Klebsiella pneumoniae превалирует при нозокомиальных формах инфекций мочевыводящих путей, характеризующихся осложненным течением и чаще диагностируемых у мальчиков до трехмесячного возраста. Одной из причин повторных эпизодов инфекций мочевыводящих путей является формирование антибиотикорезистентности за счет действия β-лактамаз микроорганизмов к наиболее часто используемым антибактериальным препаратам группы аминопенициллинов. В литературном обзоре обоснован выбор антибактериальной терапии инфекций мочевыделительной системы в детском возрасте с учетом клинической эффективности, безопасности и чувствительности основных уропатогенов. Обобщены результаты рандомизированных клинических исследований эффективности и безопасности использования цефалоспорина III поколения цефиксима у детей с инфекциями мочевыделительной системы. Особое внимание уделено сохранению бактериальной чувствительности на уровне свыше 90 % к действию цефиксима у Escherichia coli и Klebsiella pneumoniae и высокой комплаентности при возможности однократного ежедневного перорального применения в качестве самостоятельной терапии на амбулаторном этапе и потенциального перехода от парентерального использования цефалоспоринов III поколения к менее инвазивному — пероральному приему, особенно у детей.

Analysis of the course and outcomes of pregnancy in patients with advanced stages chronic kidney disease

Pregnancy in patients with advanced chronic kidney disease (CKD) is associated with a high risk of adverse outcomes for the mother and the fetus, but data on the characteristics of the course of pregnancy in these women is limited. To analyse of the course and outcomes of pregnancy in patients with CKD stages 3a4. Thirty five pregnant women with CKD stages 34 were included: 3a 12 (34.3%) patients, stage 3b 10 (28.6%), stage 4 13 (37.1%). Proteinuria, serum creatinine, blood pressure in dynamics, the presence of a physiological response were investigated. Pregnancy management included blood pressure correction, antianemic, antiplatelet, anticoagulant therapy, prevention and treatment of urinary infection, correction of metabolic disorders. All pregnant women had proteinuria of varying severity, which increased towards the end of pregnancy. Seventeen (51.5%) patients had hypertension, successfully corrected with antihypertensive drugs. The average delivery term was 34.6 weeks. Preeclampsia developed in 14 (42.4%) cases, an inverse relationship was found between the presence of a physiological response and preeclampsia (p=0.009; rs=-0.463). All children were born alive and viable. After delivery in patients with CKD 3a creatinine values returned to the pre-gestational level, in patients with grade 3b and 4 progression of CKD was noted. A favorable pregnancy outcome in women with late stages of CKD is possible with constant monitoring by a multidisciplinary team of doctors with mandatory monitoring of renal function, proteinuria, blood pressure, coagulation, markers of preeclampsia and indicators of fetal health. It was proposed to consider the physiological response of the kidneys to pregnancy as a predictor of a favorable outcome.

Rational use of antibiotics for treatment of urinary infection in sows

Abstract The endemic character of urinary infections (UI) in sows makes collective antimicrobial therapies via feed a routine. This, however, generates sub-doses unable to heal and contribute to the selection of antibiotic-resistant bacteria. The use of individual therapy is the most appropriate procedure to be performed on animals with UI. With this study, we aimed to evaluate the occurrence of UI in sows housed in the western region of Paraná and the efficacy and cost-benefit of individual treatment. A total of 353 females were selected from five different herds, submitted to urine collection in the final third of pregnancy by spontaneous urination method. The samples were analyzed physically and chemically with the use of reagent strips, and the presence of nitrite was a determinant for positivity for UI. The animals with UI had urine submitted to a bacteriological evaluation, were treated with parenteral medication (marbofloxacin - single dose - 8 mg/kg), and submitted to a new urine collection 24h and 48h after the first. UI was observed in 4.53% of the females evaluated (16/353). Escherichia coli and Streptococcus sp. were the most frequently isolated agents. Seven days after the use of marbofloxacin 87.5% (14/16) of the animals were negative for UI, which demonstrates the efficacy of UI parenteral control. The diagnosis associated with individual therapy at the expense of collective medication was highly cost-effective, made it possible to drastically reduce the number of medicated animals, and was efficient in controlling UI. Thus, it is concluded that it is possible to make rational use of antibiotics by treating only sows that are proven to be positive for UI. This reduces the number of unnecessarily medicated animals and reduces the cost due to the use of antimicrobials only in sick animals.

Association Between Urinary Tract Infection in the First Trimester and Risk of Preeclampsia: A Case-Control Study.

BackgroundUrinary tract infection (UTI) is a common clinical problem during pregnancy that can have serious consequences for the mother and fetus. Some studies have suggested that UTI can trigger or aggravate preeclampsia. The present study aimed to investigate the association between urinary tract infection in the first trimester and the risk of preeclampsia in pregnant women referring to Bahar hospital of Shahroud.Patients and MethodsIn this case–control study, 92 pregnant women with a diagnosis of preeclampsia were selected as cases, and for comparison 92 pregnant women were selected as control. History of previous UTI in the first trimester was assessed as a risk factor. Data were analyzed using SPSS 16 software and related statistical tests such as mean and standard deviation, chi-square, and independent t-test.ResultsIn this study, the mean age of the patients was 28.6 ± 6.9 years that no significant differences were found between the two groups. It was also found that 37 (40.2%) patients in the case group and 29 (31.5%) patients in the control group had a UTI which was significantly (p<0.043) higher in the case group. Also, in the multivariate regression model, UTI was significantly associated with preeclampsia (p<0.048), so that UTI increases the risk of preeclampsia (OR=1.86).ConclusionThe results of this study showed UTI during the first trimester of pregnancy is associated with the risk of preeclampsia. Therefore, controlling and treatment of urinary infections can reduce the risk of preeclampsia in the later months.

Standardization of diagnosis and treatment of urinary infection in the female, maintenance of female physical health and well-being

Standardization of diagnosis and treatment of urinary infection in the female, maintenance of female physical health and well-being

Preliminary Phytochemical Screening and Antimicrobial Activity of Salvadora persica Linn. Extracts against Oral Pathogens

Salvadora persica Linn. is used traditionally as hypotensive, emollient, laxative, diuretic, febrifuge, skin cleanser and in treatment of urinary infections, gall stones, bronchial asthma and diarrhoea. The present study was designed for evaluation of phytochemical and anti-microbial aspects of S. persica stem and bark extracts against oral pathogens. The antimicrobial screening of extracts was examined by agar well diffusion method at 200 mg/ml sample concentration and minimum inhibitory concentrations (MICs) by two fold serial dilution method. Ofloxacin was used as positive control to determine the sensitivity of the strains. The results showed that MeOH extract was more active than other extracts in its antimicrobial activity ranged 14.0 ± 0.50-22.3 ± 0.76 mm respectively. MIC values were recorded between 3.12 to 25 mg/ml for all selected pathogens. Phytoconstituents analysis of plant extract revealed the presence of alkaloids, flavonoids, glycosides, steroids, tannins, terpenoids and saponins. The results corroborate the traditional uses of S. persica in treatment of dental diseases.

Cycloserine as an alternative urinary tract infection therapy: susceptibilities of 500 urinary pathogens to standard and alternative therapy antimicrobials

Cycloserine has been used previously in some areas of the world for the treatment of urinary tract infections. The emergence of multi-resistant strains of Enterobacteriaceae and the lack of new agents in the development pipeline has prompted a need to review the activity of older agents. Susceptibility testing of cycloserine has traditionally been problematic owing to testing in standard media, containing competitive alanine, thus presenting falsely elevated minimum inhibitory concentrations (MICs). This study tests urinary coliforms against cycloserine in both standard and minimal media. Susceptibilities were performed on 500 "wild type" UTI coliforms using Mueller-Hinton broth in the range 0.008-128 μg/ml in accordance with ISO guidelines. Cycloserine was also tested in Minimal Salts medium + 2 % 1 M glucose + 0.2 % 1 M magnesium sulphate. MICs were recorded after 18 h of incubation at 35 °C and interpreted with EUCAST breakpoints (where available). Cycloserine MIC50 for the "wild type" coliforms was 32 μg/ml in Mueller-Hinton broth compared with 2 μg/ml in Minimal Salts. Eighty-seven per cent of "wild type" UTI coliforms show cycloserine MICs < = 8 μg/ml in Minimal Salts. The epidemiological cut-off values for cycloserine for E. coli in this study were 64 μg/ml using Mueller-Hinton broth and 8 μg/ml using Minimal Salts medium. Ninety-four per cent of trimethoprim-resistant and 82 % of third generation cephalosporin-resistant E. coli had MICs in Minimal Salts ≤ 8 μg/ml. Cycloserine is still licensed in some countries for the treatment of urinary infections and the data presented here suggest that it may play a role in the management of infections resistant to trimethoprim and third generation cephalosporins.

&amp;lt;i&amp;gt;In Vitro&amp;lt;/i&amp;gt; Evaluation of Ozone Activity on Recent Clinically Isolated Bacterial Strains

This study aims to evaluate the cozone bactericidal activity in different suspension media (saline, broth and whole blood) at different exposure times. Methicillin-resistant Staphylococcus aureus, Enterococcus faecalis, ESBLpositive Escherichia coli, MDR Pseudomonas aeruginosa were suspended in different media. We used a bacterial concentration of 0.2 MF for all experiments, as this concentration is consistent with the results of septic shock blood experiments. We performed ozone insufflations in a “sealed environment”. The total number of insufflations for each experiment ranged from one to four. The gas concentration was maintained at 80 mcg/ml. We confirmed the bactericidal activity of ozone on saline for all the bacterial strains. Experiments in broth revealed no changes in the bacterial growth. Ozone is primarily bactericidal against E. coli and bacteriostatic on P. aeruginosa, S. aureus and E. faecalis on whole blood. This study confirms the bactericidal efficacy of topical ozone applications and supports the need for further evaluations of the therapeutic potential of major ozone autohemotherapy. The results in E. coli promote further investigations of ozone activity on other Enterobacteriaceae and its potential use in the treatment of urinary infections. In general, these results suggest that ozone-therapy might be an alternative therapy to overcome antibiotic resistance.

Petechiae- adverse reactions to Ciprofloxacin

Sir There are many dermatological adverse reactions to Ciprofloxacin reported in the literature: allergic reaction, pruritus, urticaria, photosensitivity/phototoxicity reaction, flushing, fever, chills, angioedema, edema of the face, neck, lips, conjunctivae or hands, cutaneous candidiasis, hyperpigmentation, erythema nodosum, sweating [1-5]. We report a case of petechiae appeared 24 hours after the initiation of Ciprofloxacin treatment for urinary infection,with long lasting evolution, despite the immediate withdrawal of the medication.The petechiae were present on the lower limbs, with intense pruritus, no systemic reactions.

In vivo study of four preparative extracts of Clematis terniflora DC. for antinociceptive activity and anti-inflammatory activity in rat model of carrageenan-induced chronic non-bacterial prostatitis

The present study was conducted to evaluate the anti-inflammatory and antinociceptive activities of Clematis terniflora DC. extracts and fractions and to further support its traditional use as Chinese folk medicine in treatment of urinary infections, especially the disease of prostatitis. The antinociceptive activity of its water extract (WE), 70% ethanol extract (EE), water eluted part of EE from AB-8 macroporous resin (WEPMR) and 70% ethanol eluted part of EE from AB-8 macroporous resin (EEPMR) was conducted using mice writhing test with different doses. Then the anti-inflammatory activity of the four parts was evaluated on rat models of carrageenan-induced chronic non-bacterial prostatitis (CNP). Preliminary study was taken to determine the phytochemical compositions of the four preparative extracts. Significant writhing inhibitory effect was found with EE at small (7.5 g/kg body wt.), moderate (15 g/kg body wt.) and large (30 g/kg body wt.) doses (doses here are presented as crude herbs) as well as EEPMR at moderate and large doses by oral administration (OA) (p≤0.01). Data from prostatic index, lecithin microsome density and white blood cell level showed that moderate dose of EE and EEPMR both had significant (p≤0.05 or p≤0.01) inhibition effect on carrageenan-induced inflammation in rat prostate. The HPLC analytical results showed that flavonoids were the main active compounds in WE, EE and EEPMR. And most flavonoids were accumulated into the part of EEPMR by AB-8 macroporous resin leaving only few compounds in WEPMR. No acute toxicity was identified in oral administration of the four parts at a dose of 100g/kg body wt. The results described here suggest that extracts of the aerial part of Clematis terniflora DC. might be of therapeutic interest in the treatment of prostatitis.

Preliminary studies on diuretic effect of hygrophila auriculata (schum) heine in rats

Purpose: Hygrophila auriculata is a traditional folk medicine widely used in the treatment of urinary infection, gout, hepatic obstruction and as a diuretic. This study was conducted to examine the diuretic effect of whole plant extracts and its fractions. Methods: The diuretic effect was examined by treating different groups of wistar albino rats with single (200 mg/kg) oral doses of alcoholic extract/fractions. Frusemide (10 mg/kg) was used as positive control in the study. The urine volume and electrolyte concentration (Na+, K+ and Cl-) were measured. Results: Out of the different fractions and extract, the n-butanol fraction (200 mg/kg) significantly and markedly increased the urine output (p < 0.01). The pattern of diuresis induced by the n-butanol fraction was almost similar to that produced by the frusemide. Conclusion: Hygrophila auriculata plant extract posesses diuretic effects in rats. Keywords: Hygrophila auriculata, Diuretics, Electrolytes, Terpenoid.

Valuation of anti-inflammatory and antinociceptive activities of Erica species native to Turkey

Erica L. species (Ericaceae) have been popularly used as antirheumatic, diuretic, astringent and treatment of urinary infections. In order to evaluate this information, anti-inflammatory and antinociceptive activities of different extracts prepared with methanol, chloroform, ethyl acetate, n-butanol and water from the aerial parts of Erica arborea L., Erica manipuliflora Salisb., Erica bocquetii (Peşmen) P.F. Stevens and Erica sicula Guss. subsp. libanotica (C.&W. Barbey) P.F. Stevens (Ericaceae) of Turkish origin were investigated by using in vivo methods. For the anti-inflammatory activity, carrageenan-induced hind paw edema model, PGE 2-induced hind paw edema model, and 12- O-tetradecanoyl-13-acetate (TPA)-induced mouse ear edema model and for the antinociceptive activity p-benzoquinone-induced writhing test in mice were employed. The ethyl acetate extracts of Erica arborea (EAE), Erica bocquetii (EBE) and Erica manipuliflora (EME) exhibited notable inhibition against carrageenan-induced (24.1–32.3%, 23.8–36.1%, 29.2–35.1%, respectively) and PGE 2-induced (21.2–37.7%, 6.8–29.7%, and 6.2–34.1%, respectively) hind paw edema as well as TPA-induced mouse ear edema models in mice, while the ethyl acetate extract of Erica sicula subsp. libanotica (ESE) (10.7–29.7%) displayed potent anti-inflammatory activity only on the PGE 2-induced hind paw edema model. However, the remaining extracts were found to be inactive against inflammatory models. Same extracts, i.e., EAE, EBE and EME were also found to exhibit remarkable antinociceptive activity in p-benzoquinone-induced abdominal constriction test at a dose of 100 mg/kg (46.5%, 27.7% and 36.3%, respectively).

Comparison of antimicrobial activity of traditionally used herbal uroantiseptics against clinical isolates of uro-genital pathogens

In present study, we compared the antimicrobial activity of ethanolic extracts of four traditionally used drugs in the treatment of urogenital infections (Ericaceae flos, Myrtilli folium, Uvae ursi folium, Vitis ideae folium) [1] with arbutin, hydrochinone, and the commonly used uroantiseptic norfloxacin. The activity against clinical isolates of the most common urinary pathogens was evaluated against gram-negative (Escherichia coli p-fimbriae positive-strains, Proteus mirabilis, Klebsiella pneumoniae, Pseudomonas aeruginosa) and gram-positive bacterial species (Enterococcus faecalis, Staphylococcus aureus), as well as against the yeast Candida albicans, using hole plate diffusion and broth dilution methods. In general, the activity of the extracts was species-dependent. In hole plate diffusion method, the extracts of Uvae ursi folium and Myrtilli folium showed stronger bactericidal activity than the rest of the extracts with average inhibition zones of 28.47 and 25.47mm, respectively. In the same method none of the extracts showed noticeable antifungal activity against Candida albicans. Using broth dilution method minimal inhibitory concentrations (MICs) were determined. All the investigated bacterial species were sensitive to the extracts with MICs below or equal to 10.42mg/mL while the MIC values for the isolates of C. albicans yeast were between 8.33 and 25mg/mL. The highest activity was demonstrated by Uvae ursi folium extract against E. faecalis (MIC 2.60mg/mL) and P. aeruginosa strains (3.13mg/mL). The antimicrobial activity of the extracts was lower than the activity of hydrochinone and norfloxacin, but higher than arbutin. Statistical analysis did not reveal clear correlation between content of arbutin, methylarbutin, polyphenols or tannin in extracts and in vitro MIC values. However, the extracts with higher amount of investigated substances tended to be more active in both methods. The results of the study confirm the justifiability of use of these drugs in treatment of urinary infections caused by investigated bacterial species.