PG Treatment Research Articles

The effect of protein-glutaminase from Chryseobacterium proteolyticum on physicochemical and functional properties of high-temperature soybean meal protein

Protein-glutaminase (EC 3.5.1.44, PG) can significantly improve the functional properties of food proteins. However, the low yield of PG has limited industrial applications. Results showed that 0.02 % tea saponin could increase the PG yield by 18.93 %. The transcription level of the PG gene was significantly enhanced, which promoted the extracellular secretion of PG through an increase in membrane permeability. On this basis, PG was used to modify high-temperature soybean meal protein (HSMP) due to its poor properties. In this study, the deamidation degree (DD) of PG-modified HSMP was optimized to 58.61 % by the response surface method. HSMP with different DD was prepared and its physicochemical and functional properties were studied. After PG treatment, the intermolecular repulsive force of HSMP increased, the particle size distribution became uniform, and the solution system was more stable. In addition, the surface morphology of HSMP gradually became loose and porous. The solubility of HSMP significantly improved, reaching 11.34 times that of untreated HSMP at pH 5.00. Meanwhile, the emulsifying and foaming capacity of HSMP significantly improved, but the foaming stability was reduced.

Срединная кольпорафия как способ коррекции пролапса гениталий у пациенток пожилого и старческого возраста.

Introduction. The problem of genital prolapse (PG) remains relevant due to its high prevalence, reaching its maximum frequency in elderly women. When choosing surgical treatment of PG in the elderly and senile, it is necessary to take into account the presence of extragenital pathology and the somatic status of the patient. One of the methods of PG correction is median colporaphy, which has a high safety profile and a low recurrence rate. The aim of the study was to evaluate the results of median colporaphy in elderly and senile patients with genital prolapse. Materials and methods. The study included 40 women aged 65 years and older with symptomatic PG III-IV degree (POP-Q), who did not have sexual intercourse and did not seek it in the future, who underwent median colporaphy using the Lefor-Neugebauer technique in combination with colpoperineolevatoroplasty. The results of surgical treatment were evaluated according to the following criteria: duration of surgery, intra- and post- operative complications, assessment of the degree of PG before and after surgery, functional and anatomical results of surgery, duration of hospital stay, assessment of symptoms of PG before and after treatment. The follow-up period for patients is from 1 to 5 years after surgery. Results. The average duration of surgery is 41.5±9.6 minutes. The average volume of intraoperative blood loss was 55.5±20.1 ml. One intraoperative complication was registered – damage to the bladder wall. The average length of hospital stay is 5.6±1.0 days. No recurrence of PG was noted. Ac- cording to The Overactive Blade Questionnaire (OAB-q), there was an improvement in the average score from 9.9+6.0 to 7.3+2.7, which indicated a decrease in the severity of PG symptoms. The frequency of dysuric phenomena after surgery decreased by 2.2 times. Urinary disorders after surgery were detected in 15 (37.5%) patients; stress urinary incontinence – in 9 (22.5%) patients, in 6 (15.0%) – de novo, imperative urinary incontinence de novo – in 1 (2.5%) case. According to the Pelvic Floor Distress Inventory questionnaire (PFDI-20), there was a decrease in symptoms and func- tional disorders of the bladder and intestines in patients: a decrease in the number of points on the POPDI-6 scale from 62.1+18.9 to 1.4+3.7; CRAD-8 – from 18.0+14.8 points to 0.7+2.1; UDI-6 – from 29.9+23 to 7.1+10.2. According to an oral survey, all 40 (100%) patients would agree to undergo this operation again and not a single patient regretted the loss of sexual function. Conclusions. Median colporaphy is a safe and effective way to correct genital prolapse in elderly and senile women, including those with a burdened somatic status, who agree to give up vaginal intercourse. It is necessary to carefully inform patients about the irreversibility of vaginal obliteration and about the possible occurrence of dysuric phenomena after surgery.

Induced Breeding of Endangered Labeo calbasu (Hamilton, 1822) Under a Hatchery System

Experiment was conducted on the effectiveness of two inducing hormones (Ovaprim and PG) on induced breeding of Labeo calbasu, an endangered Kalibaus species. From April to July of 2022, Labeo calbasu were bred artificially. For the purpose of induced breeding, male and female brood fish weighing between 1.2 kg and 2.0 kg for the male and 1.4 kg and 2.5 kg for the female were chosen, and the sex ratio was maintained at 1:1. PG and LHRH-A were used in the experiment’s design for the period of April, May, June, and July. Under controlled conditions, the impact of two hormones sources on L. calbasu ovulation, fertility, and hatching rate was studied. A single dose of Ovuline® (LHRH-A) (0.44 -0.50 ml/kg body weight) plus two doses of PG (an initial dose of 2.0 mg/kg body weight and a final dose of 4.0-4.5 mg/kg body weight) showed better results in this group of females. In the period of May and June, the ovulation, fertility, and hatching rate of L. calbasu were all better in both PG and LHRH-A. Ovuline® (LHRH-A) 0.15 ml/kg body weight and PG 2 mg/kg body weight provided as a single dose to males resulted in superior spermiation outcomes. Fish weighing 1.55±0.86 kg had the highest GSI value (22.82%) and fecundity (501301±9.96), whereas fish weighing 1.51±0.96 kg had the lowest GSI value (18.24%) and fecundity (350561±8.76). The treatment of PG showed the highest rates of fertilization (98.40±1.17%) and hatching (88.03±2.76%), whereas the treatment of LHRH-A showed the highest rates of fertilization (98.64±0.84%) and hatching (89.55a ±0.88%). According to the results of this experiment, PG and ovaprim are equally effective at causing L. calbasu ovulation, fertilization, and hatching. Both PG and LHRH-A could be applied by hatchery operators to enhance L. calbasu breeding performance by inducing better spawning.

Lipolysis inhibition as a treatment of clinical ketosis in dairy cows: A randomized clinical trial

Excessive and protracted lipolysis in adipose tissues of dairy cows is a major risk factor for clinical ketosis (CK). This metabolic disease is common in postpartum cows when lipolysis provides fatty acids as an energy substrate to offset negative energy balance. Lipolysis in cows can be induced by the canonical (hormonally induced) and inflammatory pathways. Current treatments for CK focus on improving glucose in blood (i.e., oral propylene glycol [PG], or i.v. dextrose). However, these therapies do not inhibit the canonical and inflammatory lipolytic pathways. Niacin (NIA) can reduce activation of the canonical pathway. Blocking inflammatory responses with cyclooxygenase inhibitors such as flunixin meglumine (FM) can inhibit inflammatory lipolytic activity. The objective of this study was to determine the effects of including NIA and FM in the standard PG treatment for postpartum CK on circulating concentrations of ketone bodies. A 4-group, parallel, individually randomized trial was conducted in multiparous Jersey cows (n = 80) from a commercial dairy in Michigan during a 7-mo period. Eligible cows had CK symptoms (lethargy, depressed appetite, and milk yield) and hyperketonemia (blood β-hydroxybutyrate [BHB] ≥1.2 mmol/L). Cows with CK were randomly assigned to 1 of 3 groups where the first group received 310 g of oral PG once per day for 5 d; the second group received PG for 5 d + 24 g of oral NIA once per day for 3 d (PGNIA); and the third group received PG for 5 d + NIA for 3 d + 1.1 mg/kg i.v. FM once per day for 3 d (PGNIAFM). The control group consisted of cows that were clinically healthy (HC; untreated; BHB <1.2 mmol/L, n = 27) matching for parity and DIM with all 3 groups. Animals were sampled at enrollment (d 0), and d 3, 7, and 14 to evaluate ketone bodies and circulating metabolic and inflammatory biomarkers. Effects of treatment, sampling day, and their interactions were evaluated using mixed effects models. Logistic regression was used to calculate the odds ratio (OR) of returning to normoketonemia (BHB <1.2 mmol/L). Compared with HC, enrolled CK cows exhibited higher blood concentrations of dyslipidemia markers, including nonesterified fatty acids (NEFA) and BHB, and lower glucose and insulin levels. Cows with CK also had increased levels of biomarkers of pain (substance P), inflammation, including lipopolysaccharide-binding protein, haptoglobin, and serum amyloid A, and proinflammatory cytokines IL-4, MCP-1, MIP-1α, and TNFα. Importantly, 72.2% of CK cows presented endotoxemia and had higher circulating bacterial DNA compared with HC. By d 7, the percentage of cows with normoketonemia were higher in PGNIAFM = 87.5%, compared with PG = 58.33%, and PGNIA = 62.5%. At d 7 the OR for normoketonemia in PGNIAFM cows were 1.5 (95% CI, 1.03-2.17) and 1.4 (95% CI, 0.99-1.97) relative to PG and PGNIA, respectively. At d 3, 7, and 14, PGNIAFM cows presented the lowest values of BHB (PG = 1.36; PGNIA = 1.24; PGNIAFM = 0.89 ± 0.13 mmol/L), NEFA (PG = 0.58; PGNIA = 0.59; PGNIAFM = 0.45 ± 0.02 mmol/L), and acute phase proteins. Cows in PGNIAFM also presented the highest blood glucose increment across time points and insulin by d 7. These data provide evidence that bacteremia or endotoxemia, systemic inflammation, and pain may play a crucial role in CK pathogenesis. Additionally, targeting lipolysis and inflammation with NIA and FM during CK effectively reduces dyslipidemia biomarkers, improves glycemia, and improves overall clinical recovery.

Combining infiltration hole and mulching techniques with fish-scale pits effectively improved soil water storage in semiarid areas with shallow buried bedrock (Pisha Sandstone) in China

Effectively regulating slope runoff and infiltrating maximum rainwater are essential to reduce soil and water loss, and promote vegetation restoration in the Pisha Sandstone areas of China, which are semiarid areas with shallow buried bedrock. However, the effects of individual or combined rainwater regulation measures, such as infiltration holes, mulch, and fish-scale pits (a semicircle-shaped pit), on soil water infiltration and restoration in such regions have not yet been fully explored. In this study, the responses of soil water to rainfall in the 0–1.6 m soil layer was investigated through in situ monitoring during the two main rainy seasons under four rainwater regulation treatments in the Pisha Sandstone area of China covered in thin soil layer (0–0.3 m): fish-scale pits with grass mulching and infiltration holes (PGH), fish-scale pits with grass mulching (PG), fish-scale pits without mulching (P), and slope land (control, CK). The results have shown that because of the relatively low permeability (0.08–0.17 m d−1) of Pisha Sandstone, individual torrential rainfall events (63.2 mm) increased the soil water only in the 0–0.3 m layer after 3 days under the CK, P, and PG treatments. However, the PGH treatment simultaneously and significantly increased the soil water in the 0–0.3 m and 0.7–0.8 m layers. Compared with the CK treatment, rainwater replenishment depths under the P, PG, and PGH treatments increased from approximately 0.4 m to 0.9 m, 1.0 m, and 1.1 m, respectively, and the average soil water storage increased by 27 mm, 34 mm, and 50 mm, respectively, in the 0–1.6 m soil layer. Moreover, the PGH treatment enhanced rainwater storage in deep soil (0.6–0.9 m), which decreased the potential for water loss by evaporation. We concluded that the PGH treatment was effective in increasing soil water storage and reducing soil erosion in areas with shallow buried bedrock.

Piceatannol-3′-O-β-D-glucopyranoside attenuates colistin-induced neurotoxicity by suppressing oxidative stress via the NRF2/HO-1 pathway

BackgroundMultidrug-resistant Gram-negative bacteria are the most pressing problem in treating infectious diseases. As one of the primary drugs for multidrug-resistant Gram-negative bacteria, the neurotoxicity of colistin has become a significant challenge in clinical practice. PurposeThis study aimed to investigate the potential effect of piceatannol-3′-O-β-D glucopyranoside (PG) on colistin-induced neurotoxicity and the underlying mechanism. MethodsIn vitro, nerve cell damage models were established by exposing N2a cells to 400 μM colistin for 24 h. The effects of PG on cell viability, apoptosis level, and oxidative stress level were analyzed. A western blot experiment was performed to determine the NRF2 pathway, apoptosis, and autophagy-related proteins. Mitochondrial morphology and mitochondrial membrane potential were detected after staining using laser confocal microscopy. In vivo, nerve injury mouse model was established by intracerebroventricular colistin administration. Morphological changes in brain tissues were observed using HE and Nissl staining. ResultsPG significantly reduced colistin-induced neuronal apoptosis levels. The apoptosis-related protein expressions were suppressed after PG intervention. Mechanistically, PG increased the levels of antioxidant factors and decreased the levels of oxidative factors, which might be related to the activation of the NRF2 pathway. In addition, PG treatment reversed the deviations in mitochondrial morphology and membrane potential. PG suppressed autophagy levels in N2a cells, possibly because PG inhibited colistin-induced apoptosis, thus reducing the level of spontaneous protective autophagy in cells. Nrf2 knockdown N2a cell models were applied to confirm that the activation of the NRF2 pathway played a vital role in PG alleviating the nerve damage caused by colistin. ConclusionPG is a potential treatment option for colistin-induced neurotoxicity. It mitigated colistin-induced oxidative stress-associated injury and mitochondrial damage by activating the NRF2/HO-1 pathway, thus reducing nerve cell apoptosis.

Successful long‐term treatment of pyoderma gangrenosum with secukinumab

AbstractPyoderma gangrenosum (PG) belongs to the group of classical neutrophilic dermatoses and is characterised by painful ulcerations. While the clinical features have been well described, the underlying pathological mechanisms are incompletely understood and adequate treatment remains challenging. A 63‐year‐old male patient had suffered from recalcitrant ulcerations on the back, groins and legs for over 20 years. Due to suspicion of PG, a skin biopsy of the flank was obtained, which was confirming the suspected diagnosis. Following several frustrating attempts to treat, another skin biopsy was carried out and a cytokine profile by quantitative reverse transcription polymerase chain reaction was performed. Compared to healthy skin, a notable upregulation of interleukin (IL)‐17A and IL‐8 was detected. Subsequently, treatment with the anti‐IL17 antibody secukinumab was started. After 3 months of treatment, the PG area was 0 cm2. Due to the discontinuation of the drug by the patient typical PG lesions reappeared, all of which disappeared again within a few weeks of restarting secukinumab therapy. At month 23, the PG area was again 0 cm2 and this complete response was maintained under secukinumab therapy to this day (76 months after the onset of secukinumab therapy). This case of a patient with therapy‐resistant PG and subsequent long‐term responsiveness to anti‐IL‐17 therapy supports the potential importance of the Th17 axis in PG. Collectively, treatment of PG using IL‐17 antagonists seems to be very promising.

Investigating the chemical profile of Rheum lhasaense and its main ingredient of piceatannol-3′-O-β-D-glucopyranoside on ameliorating cognitive impairment

Rheum lhasaense A. J. Li et P. K. Hsiao, a stout herb plant from the Polygonaceae, is a typical Tibetan folk herb with heat-clearing and detoxifying effects, but does not have the typical laxative effect compared with other rhubarb plants. Nevertheless, its chemical composition and pharmacological activities still lack in-depth research. The present study endeavored to analyze the possible phytochemical constituents in R. lhasaense and explore the main compound piceatannol-3′-O-β-D-glucopyranoside (PG) effect on cognitive impairment and its underlying mechanism. The chemical profile of R. lhasaense discovered 46 compounds, including 27 stilbenoids and 13 gallotannins using UPLC-Q-TOF-MS/MS. The UPLC determined the contents of 6 main stilbenoids, among which the content of PG was the highest, up to 61.06 mg/g. Moreover, behavioral tests showed that PG (40 mg/kg and 160 mg/kg) administration markedly ameliorated memory impairments of scopolamine-induced mice. Biochemical parameters showed that PG treatment alleviated the levels of Ach, AchE, and inflammatory factors while elevating the levels of antioxidants in mice. In addition, network pharmacology was performed to reveal PG exert an mild cognitive impairment effect by participating in neurodegenerative disease pathways, proliferation and apoptosis-, and inflammation-related pathways. Eventually, the results of molecular docking and the qRT-PCR revealed that PG down-regulated the mRNA expressions of MMP3, MMP9 and BACE1 in cognitive impairment mice brain tissue. In conclusion, our results demonstrated that PG mitigated scopolamine-induced cognitive dysfunction in mice by targeting the BACE1-MMP3/9 pathway, and PG might be a promising mild AD drug candidate.

Reversal of Neuralgia Effect of Beta Carotene in Streptozotocin-Associated Diabetic Neuropathic Pain in Female Zebrafish via Matrix Metalloprotease-13 Inhibition.

Beta carotene is a natural anti-oxidant agent, and it inhibits the matrix metalloprotease (MMP) activity. Diabetic neuropathic pain (DNP) is produced by cellular oxidative stress. The role of the beta carotene effect in diabetic neuropathic pain is not explored yet. The present study is designed for the evaluation of the palm oil mill effluent-derived beta carotene (PBC) effect in DNP in zebrafish. The DNP was induced by the intraperitoneal administration of streptozotocin (STZ). Blood glucose levels of above 15 mM were considered to be diabetic conditions. The zebrafish were exposed to test compound PBC (25, 50, and 100 µM), pregabalin (PG: 10 μM), and an MMP-13 inhibitor (CL-82198; 10 μM) for 10 consecutive days from day 11. The neuralgic behavioral parameters, i.e., temperature test, acetic acid test, and fin clip test were assessed on day 0 and the 7th, 14th, and 21st days. On the 22nd day, the blood glucose and MMP-13 levels and brain thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH), and MMP-13 activity levels were estimated. The treatment of PBC ameliorated the DNP-associated behavioral and biochemical changes. The results are similar to those of PG and CL-82198 treatments. Hence, the PBC possesses a potentially ameliorative effect against DNP due to its potential anti-oxidant, anti-lipid peroxidation, and MMP-13 inhibitory actions.

Induction Spawning of Endangered Kuria Labeo, Labeo gonius (Hamilton-Buchanan, 1822) under Hatchery System

Efficacy of two inducing hormones (PG and Ovaprim) was conducted on induced breeding of endangered Kalibaus (Labeo gonius). Induced breeding of L. gonius was carried out from April to July, 2022. Male and female brood fish weighing between 0.85±0.43kg and 0.88±0.50 kg in case of male and 0.97±0.641.4kg to 0.99±0.802.5kg in case of female were selected for the induced breeding and sex ratio were maintained 1:1 for the breeding purpose. The experiment was designed with the month of April, May, June and July with PG and LHRH-A. Two hormonal sources were tested to evaluate their efficacy on ovulation, fertility and hatching rate of L. gonius under controlled conditions. Double doses of PG (an initial dose of 1.5mg/kg body weight and final dose of 4.0mg/kg body weight) and single dose of Ovuline® (LHRH-A) (0.4 -0.45ml/kg body weight) showed better results in case of females. Bothe PG and LHRH-A had shown better results of ovulation, fertility and hatching rate of L. gonius in the month of May-June. Males were administered with a single dose of Ovuline® (LHRH-A) 0.15 ml/kg body weight and PG 1.5 mg/kg body weight showed better results of spermiation. Highest GSI value (22.24%) and fecundity (300411±7.16) were found in 0.90±0.80kg body weighted fish and the lowest value of GSI (20.01%) and highest fecundity (380822±9.11) were found in 1.10±0.96kg body weighted fish. The highest fertilization rates (98.40±1.17%), hatching rates (88.03±2.76%) were recorded in treatment of PG and highest fertilization rates (98.64±0.84%), hatching rates (89.55a±0.88%) were recorded in treatment of LHRH-A. This experiment recommends Ovaprim and PG both are efficient for inducing ovulation, fertilization and hatching of L. calbasu. The hatchery operators may be used both PG and LHRH-A for induction of spawning better performance of L. gonius breeding.

PSI-6 The Influence of a Single Administration of Prostaglandin on Estrus Synchronization in Range Sheep Production

Abstract Administration of a single injection of prostaglandin after ram introduction has previously been reported as an effective method to synchronize estrus in ewes housed in a dry lot setting. The objective of this study was to investigate the effectiveness of a single injection of prostaglandin administered at the beginning of the breeding season after ram introduction, on estrus synchronization in range ewes. Western Whiteface cross ewes, of which thirty-six were ½ blood South African Meat Merino (SAMM) and seventeen ¼ blood SAMM (total n=53) were assigned to one of two treatments: control (CON; n=26) or one injection of prostaglandin (PG; n=27). Ewes were utilized in a complete randomized block design and were stratified by SAMM percentage, body condition score (BCS) and age. Ewes assigned to PG treatment were administered 3 mL of prostaglandin IM (Lutalyse, Zoetis Animal Health) approximately 60 h after rams were introduced with ewes. Rams (n=4) were applied with marking paint and placed with ewes on (d 0: December 21) and remained for the duration of the 56 d breeding season for natural service. Ewes were observed for estrus twice daily at 0730 h and 1700 h for d 0-10 and d 16-21 of the breeding season, by an experienced evaluator. At lambing, birth weight, sex, and type of birth was recorded. The MIXED and GLIMMIX procedures of SAS were used to analyze estrus response, ewes bred in the first 5 d of the breeding season, days to lambing, lambing date, number of lambs born, and lamb weight. South African Meat Merino percentage was included as an independent variable but was found to be nonsignificant and was removed from the statistical model. The proportion of ewes bred during the first 5 d of the breeding season was not different among the two treatments (P ≥ 0.60). There was no difference between the PG ewes and the CON ewes for the date of estrus (P ≥ 0.82). There was no difference between the PG ewes and the CON ewes for the lambing date (P = 0.60). Treatment did not influence days to lambing (P ≥ 0.25). The number of lambs born per ewe tended to be influenced by treatment (P &amp;lt; 0.08), with PG ewes tending to have more lambs than CON ewes. Lamb weights were not different among treatments (P ≥ 0.50). In conclusion, the administration of a single PG injection after ram exposure did not influence estrus expression, days to lambing, or lambing date, however, PG ewes did tend to have more lambs.

291 Synchronization and Seasonality Effects on Follicular Development, Estrus Expression, and Pregnancy in Brahman Females

Abstract Control of follicular development is essential for estrous synchronization. This experiment determined differences in follicular development during synchronization in Brahman females. Cows (n=219) and heifers (n=90) in Fall (n=170) and Spring (n=139) were synchronized with a CIDR insert and administration of GnRH or PG treatment on d0 (1/2GnRH: n=106, GnRH: n=103, PG: n=100), and CIDR removal and PG administration on d6 followed by estrus detection and AI for 96h. Blood samples were collected on d0, d6, and d8 and/or AI to determine circulating progesterone (P4) concentrations. Transrectal ovarian ultrasonography occurred daily. Cows were grouped by CL presence (d0CL, d6CL) and P4 concentrations (d0P4, d6P4, d8P4, Low&amp;lt; 1 ng/mL, High≥1 ng/mL) on d0 and d6. The GLIMMIX procedure of SAS was used to analyze follicular wave initiation, wave emergence day, estrus expression, and pregnancy success. There were significant interactions of treatment by d0CL (P=0.0237), and season by d0P4 (P=0.0118) on wave initiation. Cows receiving 1/2GnRH with a CL on d0 (97±2%) and Fall cows having High d0P4 (96±3%) had the greatest probability of wave initiation. Age significantly impacted wave initiation (P=0.0213; heifers: 93±4%, cows: 79±5%). Interactions of treatment by season (P=0.0405) and treatment by d0CL (P=0.0379) significantly impacted wave emergence timing. Season (P&amp;lt; 0.0001; Fall: 23±5%, Spring: 65±6%), and the interactions of treatment by d0P4 (P=0.0291) and new wave by d0P4 (P=0.0357) influenced estrus expression. Cows receiving 1/2GnRH with High d0P4 and cows with High d0P4 that initiated a new wave had the greatest probability of estrus expression. Pregnancy rate differed by estrus expression (P=0.0055; no estrus=25±6%, estrus=54±8%) and semen type (P=0.0105; conventional=52±9%, sexed=26±6%), and tended to differ by d8P4 (P=0.0570; Low=30±7%, High=48±8%). These results suggest that differences in follicular development and behavioral estrus in synchronized Brahman females may be due to seasonality and endogenous progesterone production.

PEMPHIGOID GESTATIONIS : REPORT CASE AND LITERATURE REVIEW

Pemphigoid gestationis is a gravidic dermatosis usually appearing between the 28th and 32nd week of amenorrhea. The rash is pruritic, bullous or vesiculopapular and of periumbilical topography. In addition to clinical findings, Thediagnosis is confirmed by direct immunofluorescence. If local treatment fails, systemic corticosteroid therapy should be administered. Oral corticosteroids are the therapeutic mainstay in pregnancy and postpartum.. Consequences may be maternal (threat of premature delivery), fetal (intrauterine growth retardation), and neonatal (skin rash). Through this observation and a review of theliterature the authors will try to focus on the physiopathology, clinicalprofile, immunological diagnosis, treatment and evolution of PG.

Combined effects of pectin–plant essential oil coating with vacuum packaging on the quality of large yellow croaker (Pseudosciaena crocea) during iced storage

AbstractThe study investigated the effects of pectin–plant essential oil coating combined with vacuum packaging on Pseudosciaena crocea during iced storage. Samples were immersed in sterile water (CK), 25% ethanol (E), pectin + 0.4% oregano essential oil (PO), and pectin + 0.4% ginger essential oil (PG), respectively. The results showed that compared with the CK group, PO and PG coatings could effectively inhibit the increase of total viable counts (TVC) and H2S‐producing bacteria counts by 1.25–1.64 log CFU/g and 1.18–1.37 log CFU/g, respectively. Meanwhile, PO and PG treatments could delay the increase of pH, thiobarbituric acid (TBA), and total volatile basic nitrogen (TVB‐N) value, which also inhibited the degradation rate of adenosine triphosphate (ATP) in fish. When samples treated with PO and PG, the shelf‐life of Pseudosciaena crocea could increase from 20 to 27 days and 24 days, respectively, which indicated that the combination of pectin–plant essential oil coating with vacuum packaging is a promising method for the preservation of Pseudosciaena crocea.

Effect of Planting into a Green Winter Cereal Rye Cover Crop on Growth and Development, Seedling Disease, and Yield of Corn.

Terminating winter cereal rye (Secale cereale L.) cover crops ≥10 days before planting (DBP) corn is recommended to minimize seedling disease and potential yield loss. In Iowa, cold temperatures and frequent precipitation can prevent farmers from following that recommendation and sometimes force them to plant corn while the rye plants are still green, referred to as "planting green" (PG). A field trial was established to evaluate the effect of rye termination shortly before or after corn planting on growth, seedling root disease, and yield of corn. A rye cover crop was terminated 17 and 3 DBP and 6 and 12 days after planting (DAP) corn; corn planted following no rye was included as a control. Rye biomass, C/N ratio, and N accumulation increased when terminated 6 or 12 DAP corn compared with rye terminated 17 or 3 DBP corn. Corn seedlings were taller from the PG treatments. More radicle root rot was observed when rye was terminated 3 DBP, 6 DAP, and 12 DAP corn than for the 17 DBP treatment and the no-rye control. Generally, greater Pythium clade B populations were detected on radicles and seminal roots of corn from the PG treatments. Corn populations, ears, or barren plants were not affected by the treatments. In both years, the no-rye control had the greatest corn yield and the 12 DAP treatment had the lowest yield. Our results suggest that PG increased corn seedling root disease and contributed to reduced corn yield.

Enzymatic modification of oat protein concentrate with trans- and protein-glutaminase for increased fibrous structure formation during high-moisture extrusion processing

Oat protein concentrate (OPC) was treated with transglutaminase (TG) and a combination of transglutaminase and protein-glutaminase (TG + PG), with or without preheating (95 °C, 15 min). The characteristics of the modified OPC were examined and further studied in high-moisture extrusion processing (HMEP). SDS-PAGE analysis showed that both enzyme preparations induced cross-linking between the OPC proteins and that the preheating step enhanced it. TG + PG treatments increased protein solubility and decreased the average particle size of OPC. TG treatment also decreased the particle size but had an insignificant effect on protein solubility. TG + PG 5 U treatment with preheating showed the highest RVA viscosity of 1076 mPa s. When OPC samples were studied during extrusion processing, enzyme treatments were found to affect the extrudate properties. The same OPC sample that showed the highest viscosity value, i.e. preheated TG + PG 5 U, showed also the most optimal fibrous structure formation and the strongest structure according to tensile strength results.

Reducing milking frequency from twice to once daily as an adjunct treatment for ketosis in lactating dairy cows—A randomized controlled trial

This randomized controlled trial investigated the effects of temporarily reducing milking frequency (MF) on the resolution of ketosis and milk production in dairy cows in early lactation. To detect ketosis [blood β-hydroxybutyrate (BHB) ≥1.2 mmol/L], Holstein cows were screened daily from 3 to 16 d in milk using a cow-side meter. Cows diagnosed with ketosis (n = 104) were randomly assigned to twice-daily milking (TDM) or reduced to once-daily milking (ODM) for 2 wk, then returned to twice-daily milking. Both treatment groups received a 5-d treatment of an oral propylene glycol drench (PG; 300 g) beginning on the afternoon of the diagnosis; cows received additional 5-d PG treatments if they had a ketotic test result (blood BHB ≥1.2 mmol/L) at least 4 d after finishing the first PG treatment. Blood BHB tests were conducted for the first 3 d after ketosis diagnosis, and then once every 3 d for 21 d of trial (DOT). Milk and milk component data were collected weekly for 15 wk following trial enrollment. The ODM group showed rapidly and markedly decreased blood BHB concentrations (primiparous cows: 1 DOT, 0.92 ODM vs. 1.22 TDM, 15 DOT, 0.55 vs. 0.81 mmol/L; multiparous cows: 1 DOT, 1.01 vs. 1.40, 15 DOT, 0.78 vs. 1.65 mmol/L). In addition, a logistic regression model indicated that ODM cows were less likely to have blood BHB concentrations ≥1.2 mmol/L [primiparous cows: 3 DOT: ODM 1% (95% confidence interval: 0-10%) vs. TDM 43% (30-58%), 15 DOT ODM 0% (0-0.2%) vs. TDM 22% (13-36%); multiparous cows: 3 DOT: ODM 33% (24-44%) vs. TDM 59% (48-69%), 15 DOT ODM 20.9% (13-31%) vs. TDM 64% (53-74%)]. The proportion of ODM cows that required additional treatments of PG were substantially lower than the TDM group (ODM: 39%; TDM: 64%) than the TDM cows during the initial 21-d period. However, during the 2-wk treatment period, cows in the ODM group produced 26% less milk and 25% less energy-corrected milk than the TDM cows. During wk 3 to 15, when all cows were milked twice daily, ODM cows produced less milk (-14%) and energy-corrected milk (-12%) compared with the TDM group. Milk protein percentage was greater, and milk fat percentage and linear score tended to be greater in the ODM group over 15 wk. In conclusion, a 2-wk reduction of MF in ketotic cows from twice to once daily with treatment with PG resolved ketosis and decreased blood BHB concentrations more effectively than treating TDM cows with PG alone. However, the 2-wk MF reduction had immediate and long-term (up to 13 wk after cessation of MF reduction) negative effects on milk production.

Proanthocyanidin from Vitis vinifera attenuates memory impairment due to convulsive status epilepticus

This study investigated the effects of proanthocyanidin-rich fraction (PRF) of Vitis vinifera seed extract on the markers of hippocampal-dependent memory in convulsive status epilepticus (CSE) rat model.One hundred juvenile Wistar rats were randomized into 6 groups. Group 1 (n = 10) received propylene glycol (PG 0.1 ml/100 g) intraperitoneally (i.p), while convulsion was induced in groups 2–6 (n = 18 each) using lithium (127 mg/kg i.p) and pilocarpine hydrochloride (40 mg/kg i.p). The established CSE rats in groups 2–6 received a daily treatment of PG (0.1 ml i.p), PRF (30 mg/kg i.p), PRF (20 mg/kg BW i.p), PRF (10 mg/kg BW i.p) or diazepam (5 mg/kg BW i.p) for seven days. Thereafter, they were kept untreated but with access to feed and water for 21 days. The control and CSE-treated rats were subjected to behavioral tests, while the biochemical and histomorphological evaluations of the hippocampus were done after the sacrifice. The results were presented as mean ± SEM in graphs or tables. The level of significance was considered when p < 0.05.There was significant decrease in the hippocampal-dependent memory, hippocampal weight and an increased malondialdehyde concentration following CSE. The activities of acetylcholinesterase decreased significantly in the PRF-treated CSE rats. The hippocampal glial cells and granule count increased significantly following CSE, with various neurodegenerative features in the CA1 of the hippocampus. These derangements were attenuated significantly following PRF treatment.Memory impairment following CSE may be attenuated with the administration of PRF from V. vinifera seed in rats.

The application of progressive systems in high density vannamei shrimp culture

The increase of super-intensive pond waste and the rearing period of high-density shrimps, has been reported to be a problem of environmental quality deterioration, which is accompanied by the chance of disease outbreak. Therefore, a progressive system with two phases of cultural activities, namely nursery and grow-out, is known to be an alternative in the process of avoiding the critical period between Day of Culture (DOC) 30-50, as well as increasing the chances of a successful culture. This research aims to examine the application of a progressive system, in the super-intensive culture of vannamei shrimps. The two methods used were Progressive & Non-Progressive treatments (PG & NPG). The NPG was used by directly stocking PL-10 to a grow-out pond of 1,000 m2, with a density of 500 PL/m2. However, PG (nursed for 21 days) was used by stocking PL-10 to PL-31 in 100 m2 pond with a density of 5,000 PL/m2, which were then transferred to a grow-out pond of 1,000 m2. The results showed that the PG treatment had better water quality parameters compared to the NPG. The survival rate, production, and feed conversion ratio in the PG treatment produced better performance than the NPG, with differences in the value of feeds used and yield of shrimp sales at 25.64% and 64.33%, respectively.

Porphyromonas gingivalis induces entero-hepatic metabolic derangements with alteration of gut microbiota in a type 2 diabetes mouse model

Periodontal infection induces systemic inflammation; therefore, aggravating diabetes. Orally administered periodontal pathogens may directly alter the gut microbiota. We orally treated obese db/db diabetes mice using Porphyromonas gingivalis (Pg). We screened for Pg-specific peptides in the intestinal fecal specimens and examined whether Pg localization influenced the intestinal microbiota profile, in turn altering the levels of the gut metabolites. We evaluated whether the deterioration in fasting hyperglycemia was related to the changes in the intrahepatic glucose metabolism, using proteome and metabolome analyses. Oral Pg treatment aggravated both fasting and postprandial hyperglycemia (P < 0.05), with a significant (P < 0.01) increase in dental alveolar bone resorption. Pg-specific peptides were identified in fecal specimens following oral Pg treatment. The intestinal Pg profoundly altered the gut microbiome profiles at the phylum, family, and genus levels; Prevotella exhibited the largest increase in abundance. In addition, Pg-treatment significantly altered intestinal metabolite levels. Fasting hyperglycemia was associated with the increase in the levels of gluconeogenesis-related enzymes and metabolites without changes in the expression of proinflammatory cytokines and insulin resistance. Oral Pg administration induced gut microbiota changes, leading to entero-hepatic metabolic derangements, thus aggravating hyperglycemia in an obese type 2 diabetes mouse model.