Treatment Of Diabetes In Rats Research Articles

An intelligent wearable artificial pancreas patch based on a microtube glucose sensor and an ultrasonic insulin pump

In order to improve the living standards of diabetes patients and reduce the negative health effects of this disease, the medical community has been actively searching for more effective treatments. In recent years, an artificial pancreas has emerged as an important approach to managing diabetes. Despite these recent advances, meeting the requirements for miniaturized size, accurate sensing and large-volume pumping capability remains a great challenge. Here, we present a novel miniaturized artificial pancreas based on a long microtube sensor integrated with an ultrasonic pump. Our device meets the requirements of achieving both accurate sensing and high pumping capacity. The artificial pancreas is constructed based on a long microtube that is low cost, painless and simple to operate, where the exterior of the microtube is fabricated as a glucose sensor for detecting diabetes and the interior of the microtube is used as a channel for delivering insulin through an ultrasonic pump. This work successfully achieved closed-loop control of blood glucose and treatment of diabetes in rats. It is expected that this work can open up new methodologies for the development of microsystems, and advance the management approach for diabetes patients.

Polyherbal antidiabetic drug: An approach to cure diabetes

In recent years, diabetes has become a major health concern. India is referred to as the diabetes capital of the world. There are plenty of chemical agents available to monitor and treat diabetic patients, but up to this date, no complete recovery from diabetes has been recorded. Many herbal plants with hypoglycemic properties are known from around the world as an alternative to these synthetic agents; which is a natural remedy to keep the blood sugar under control by consuming vegetables & herbs in our diet. Such medicinal plants & their herbal preparation with proven antidiabetic and related beneficial effects were used in the treatment of diabetes in rats. The effect of polyherbal drug Anti-hyperglycemic activity is studied in rats against alloxaninduced diabetes. This polyherbal drug consists of 16 antidiabetic plants. Disorders in diabetes-induced glucose metabolism have been shown to be regulated. The present study was conducted in the Dept. of Pharmacology and Dept. of Biochemistry at Central Animal House Facility of the SBH Govt Medical College, Dhule, in collaboration with Datta Meghe Medical College, (Datta Meghe Institute of Medical Sciences Sawangi, Meghe) Nagpur, Maharashtra, India. After the treatment, fasting blood glucose, plasma insulin and glycosylated hemoglobin (HbA1c) were determined in normal and experimental rats. Polyherbal mixture was seen to be an effective and safe method for management of diabetes which reduces blood sugar levels & shows positive effect in altering blood glucose levels. The herbal formulation could be lowering the insulin resistance, thereby normalizing the uptake of glucose by cells.

The Preparation of Hyaluronic Acid Nanoparticles from Aspicilia lichens Using Bifido Bacteria for Help in the Treatment of Diabetes in Rats In Vivo.

Many common herbs and spices are claimed to have blood sugar lowering properties that make them useful for people with or at high risk of diabetes. The main of compounds of kiwifruit (Actinidia deliciosa), rhubarb (Rheum ribes), membrane inner of egg shell, wool of sheep, human fingernail (unguis), hyaluronic acid produced by the Bifidobacterium, and usnic acid of Aspicilia lichen were extracted by different methods. All compounds of the extract were divided into five groups. We used variables such as pH, different compounds, concentration, number of injections, and blood glucose monitoring in different situations. Our study, extracts changed to nanoform. The extract compounds and nanoparticles were analyzed by gas chromatography mass spectroscopy, Fourier transform infrared spectroscopy, hydrogen-1 nuclear magnetic resonance, and scanning electron microscope. The average size of the nanoparticles was found to be 55nm. Five groups of nanoparticles were injected into rats, and they reduced their blood glucose levels significantly (statistical significance was declared at p<0.05). The synthesized hyaluronic acid helped to treat diabetes in rats. Copyright © 2017 John Wiley & Sons, Ltd.

Pharmacological Activity of Some Nigeria Plants Extracts in the Remidiation of Alloxan-Induced Diabetes in Rats: A Review

Plants are considered to be medicinal if they possess pharmacological activities of possible therapeutic use. A narrative perspective of medicinal evidences on the biochemical effectiveness of plant extracts used in the treatment of diabetes in rats was reviewed. The review was designed to highlight the chemical constituents and pharmacological potentials of some Nigeria plants used in experimental diabetes. The literature survey reveals the therapeutic efficiency of crude aqueous extracts of many plant species used either independently or in combination with some standard drugs for the treatment of diabetes with rats. Identified literature show a considerable degree of overlap and consistency in methods and results of findings. Some plant extracts were reported to be more effective in combination with other plant extracts and also a few were more effective than many standard drugs. There is a good number of quality research regarding plant extracts for the treatment and management of diabetes in rats. These plants identified as having anti diabetic potentials may be remedy for the treatment and management of diabetes in human. Research should explode more about these valuable plants to ascertain the dosage that may be required for man and if possible develop novel drug molecules for the future.

Additive effect of ozone therapy to insulin in the treatment of diabetic rats

Background Chronic hyperglycemia of diabetes is associated with long-term damage, dysfunction, and failure of various organs, especially the heart and blood vessels. Therapy in the past few decades was mainly aimed at reducing hyperglycemia. It became clear that ameliorating oxidative stress through treatment with antioxidants might be an effective strategy for reducing diabetic complications. Medical ozone treatment may be useful in the treatment of diabetes and its complications. Objective This study aims to show the additive effect of ozone to insulin in the treatment of diabetes in rats. Materials and methods Diabetes was induced by an intraperitoneal injection of streptozotocin (45 mg/kg) in 0.2 ml of 10 mmol/l citrate buffer. Rats were considered diabetic when fasting blood glucose was at least 113 mg/dl. Rats proved to be diabetic were isolated and subdivided into four subgroups: (a) diabetic nontreated rats (n = 8), (b) diabetic ozone-treated rats (n = 8), (c) diabetic insulin-treated rats (n = 8), and (d) diabetic insulin + ozone-treated rats (n = 8). After induction, all rats were fasted for 12 h. Systolic blood pressure (SBP) was measured. Retro-orbital blood samples were collected for estimation of fasting serum glucose, glycosylated hemoglobin, total antioxidant capacity (TAC), and malondialdehyde (MAD) level. Rats were then sacrificed; vascular reactivity to norepinephrine and acetylcholine with and without endothelial lining was estimated. Results The data showed that insulin reduced the elevated fasting serum glucose, glycosylated hemoglobin, MAD, and SBP significantly when compared with the diabetic nontreated group. Also, it significantly reduced TAC and vascular reactivity to norepinephrine with and without endothelium, but there was an increase in the percent of relaxation to acetylcholine. Ozone therapy potentiated the effects of insulin on SBP and vascular reactivity. Importantly, serum MAD and TAC and glycemic state were significantly improved. Conclusion This study shows that ozone therapy may have an additive effect in the treatment of diabetes by insulin; this may attribute to the multiprotective antioxidant effect of ozone.

Ileal interposition for the treatment of diabetes in rats: repercussion on beta cells mass

Objetivo: Pesquisa com o objetivo de estudar a açáo da interposiçáo do íleo terminal na hiperglicemia e na massa de células beta pancreáticas em ratos diabéticos náo obesos. Métodos: Foram utilizados de 18 ratos Wistar, divididos aleatoriamente em 3 grupos de 6 cada. Um grupo controle náo diabético, um grupo (controle diabético) submetido à induçáo do diabetes com estreptozotocina, e outro grupo diabetes submetido à interposiçáo ileal. A massa de células beta foi quantificada por método indireto através da divisáo dos níveis séricos de peptídeo-C (ng/mL) pela glicemia (mg/dL). Usados os testes de variância (ANOVA) e Tukey, para análise de diferenças específicas, considerando-se significância de p&lt;0.05. Resultados: A glicemia dos animais diabéticos com interposiçáo ileal foi significativamente reduzida (94,5±5.6mg/dL), quando comparada com o grupo controle diabético (245±4,8mg/dL) (p&lt;0,05). O peptídeo-C revelou níveis significativamente maiores nos animais do grupo diabetes interposiçáo (0,58±0,06ng/mL) do que no grupo controle diabetes (0,42±0,03ng/mL), sugerindo maior resposta à estimulaçáo pancreática neste grupo (p&lt;0,05). A razáo entre peptídeo-C e glicemia revelou diferença significante (p&lt;0,05), observando-se maior massa e funçáo de células beta nos animais diabetes interposiçáo (0,61) do que nos controle diabetes (0,004). Conclusáo: Os resultados obtidos permitem concluir que a interposiçáo ileal contribuiu para a reduçáo da glicemia e para o aumento da massa de células beta funcionantes em ratos diabéticos.