Treatment Of Chromatograms Research Articles

Use of Computer-Assisted Color Treatment of Chromatograms in the TLC Determination of Tartrazine

A procedure was developed for the treatment of thin-layer chromatograms in the analysis of the Tartrazine E102 synthetic food dye. The procedure includes the conversion of a TLC plate into the Windows 95/98 bit-mapped graphics format using scanning on a flatbed scanner followed by the processing of the image obtained with the Adobe Photoshop 5.0 program package in the RGB color mode (red, green, and blue color channels).

METABOLISM AND TRANSLOCATION OF 1,2-14C(2-CHLOROETHYL)PHOSPHONIC ACID IN PRUNUS PERSICA (L.) BATSCH

Abstract 1,2- 14 C (2-chloroethyl)phosphonic acid ( 14 C-CEPA) translocation and metabolism was studied in short-term experiments on ‘Andross’ peach trees at the end of Stage I of fruit development. The chemical was applied to the fruit surface or to the abaxial surface of the basal leaf of a developing shoot. When the tracer was applied to the fruit, no translocation of the chemical occurred. When the 14 C-CEPA was applied to the leaf, translocation was observed. Thin-layer chromatography (TLC) of the radioactivity recovered in acidic methanol extracts of both fruits and one-year shoots, collected 24, 48 and 96 h after 14 C-CEPA application to the leaf, indicated that the translocation occurred in forms different from the original chemical. Treatment of chromatograms with AgNO 3 and FeCl 3 at 100°C showed that the colour reaction of sugars was associated with the radioactive areas. Therefore it seems likely that the translocation of 14 C-CEPA occurred in sugar-conjugated form. Results of the TLC of 14 C-CEPA before spotting or overspotted with sugars or fruit and leaf extracts, showed that the binding of 14 C-CEPA to sugars is not a metabolic reaction, although the complex, being more stable than the original chemical and biologically active, could have physiological significance in determining long-term responses of tissues to treatments with CEPA.

Metabolism and translocation of 1, 2- 14C(2-chloroethyl) phosphonic acid in Prunus persica (L.) Batsch

1,2- 14C (2-chloroethyl)phosphonic acid ( 14C-CEPA) translocation and metabolism was studied in short-term experiments on ‘Andross’ peach trees at the end of Stage I of fruit development. The chemical was applied to the fruit surface or to the abaxial surface of the basal leaf of a developing shoot. When the tracer was applied to the fruit, no translocation of the chemical occurred. When the 14C-CEPA was applied to the leaf, translocation was observed. Thin-layer chromatography (TLC) of the radioactivity recovered in acidic methanol extracts of both fruits and one-year shoots, collected 24, 48 and 96 h after 14C-CEPA application to the leaf, indicated that the translocation occurred in forms different from the original chemical. Treatment of chromatograms with AgNO 3 and FeCl 3 at 100°C showed that the colour reaction of sugars was associated with the radioactive areas. Therefore it seems likely that the translocation of 14C-CEPA occurred in sugar-conjugated form. Results of the TLC of 14C-CEPA before spotting or overspotted with sugars or fruit and leaf extracts, showed that the binding of 14C-CEPA to sugars is not a metabolic reaction, although the complex, being more stable than the original chemical and biologically active, could have physiological significance in determining long-term responses of tissues to treatments with CEPA.