In this paper, we proposed and demonstrated a novel switchable fiber comb filter based on polarization beam splitter (PBS). The proposed filter transmission characteristics is theoretically analyzed in detail by using the transmission matrix theory. The simulated results show that the filter can generate two different output channels selected by adjusting the polarization controllers (PC) in front of the PBS, and the two different channels’ wavelength intervals can be changed with polarization-maintaining fiber’s (PMF’s) characteristics. Different PMFs give a new switchable combination of channel intervals. A multi-wavelength fiber laser (MWFL) based on the proposed filter is experimentally demonstrated. Its wavelength interval can be switched among 0.85 nm, 0.40 nm, which is consistent with the simulated results of the filter. The power fluctuation of the two channel interval outputs in one hour is less than 0.88 dB and 1.37 dB, and the wavelength fluctuation is less than 0.06 nm and 0.18 nm, respectively.