Postmenopausal breast cancer survivors, who often experience significant weight gain after diagnosis, are at greater risk of morbidity and mortality than women without a history of breast cancer. There is evidence that mindfulness has positive effects on markers of cardiovascular health; however, little is known about changes in the biological mechanisms underlying this behavioral intervention. The objective of the study was to examine the effects of a mindful eating intervention on biomarkers of prognosis and survival in overweight and obese postmenopausal breast cancer survivors. Participants were recruited through local physician's offices, cancer support centers, and newspaper advertisements in 2016. Participants completed 8 weekly 2‐hour Mindful Eating Workshop© group sessions, led by a certified mindfulness instructor. The workshop addressed mindless, stress‐related, or emotional related eating, and disordered eating patterns through mindfulness meditation and group discussion. Data were collected at the University of Georgia Clinical and Translational Research Unit at 2 time points: baseline and follow‐up (12 weeks). The following data were collected: adiposin, adiponectin, leptin, PAI‐1, 6Ckine, MIP‐3b, MIG‐1, MCP‐1, IL‐6, IL‐8, IL‐10, and TNF‐a. Paired t‐tests and Wilcoxon signed rank test were used to compare outcomes pre‐ and post‐intervention. The analytic sample included 10 postmenopausal breast cancer survivors in remission with a BMI of ≥ 25 kg/m2 who completed the intervention. Mean age was 64.4 ± 6.0 years, 90.0% were non‐Hispanic white, 50.0% were married, and 50.0% had an annual household income of more than $80,000. At baseline, participants had a mean BMI of 33.7 ± 7.2 kg/m2, 80.0% took 1+ blood pressure medications, and 40.0% had pre/diabetes. All participants significantly lost weight (mean weight change: −0.8 ± 0.8 kg; p= 0.015). In the absence of significant changes in TNF‐a and other markers of inflammation, PAI‐1 increased (p=0.012) and there was a trend towards a decrease in adiponectin (p=0.051) post‐intervention. However, participant PAI‐1 plasma concentrations were not elevated above the reference normal concentration of 5–40 ng/ml. In addition, in 60.0% of participants adiponectin concentrations remained within normal concentrations post‐intervention. The results from this study provide preliminary evidence of the translational effects of a mindful eating intervention on biomarkers associated with prognosis and survival in postmenopausal breast cancer survivors. Larger, better controlled studies are needed to confirm findings and examine the long‐term impact of this intervention.Support or Funding InformationThe study was funded through the University of Georgia Clinical and Translational Research Unit seed grant.