Transition Cow Health Research Articles

Association of uterine health in the first lactation with transition cow health and reproductive performance in the second lactation of Holstein dairy cows

The objective of this study was to evaluate the effects of puerperal metritis (PM) diagnosed and treated during the early postpartum period of the first lactation on transition cow health, milk production, reproduction, and culling of dairy cows in their second lactation. Diagnosis of PM was based on fetid watery red-brown uterine discharge and rectal temperature above 39.5°C. Two farms were enrolled in this retrospective observational cohort study (farms A and B). In both farms, the following diseases were recorded during the first 30 DIM in lactation 1 and 2: clinical hypocalcemia (CH), retained fetal membrane (RFM), PM, hyperketonemia (KET), left displaced abomasum (LDA), and clinical mastitis (MAST). Statistical analyses were performed using SPSS for Windows separately for each farm. Linear and logistic regression models were used for continuous (e.g., milk yield) and binary (e.g., disease, pregnancy per AI, pregnancy loss) outcomes, respectively. Cox proportional hazard regression models were calculated to model the time to event outcomes for culling or death during the first 60 DIM and for pregnancy within 250 d of the second lactation. The initial models contained the following variables: year of calving, month of calving, calving ease, stillbirth, twins, days open in lactation 1, 305-d milk yield in lactation 1, PM in lactation 1, and PM in lactation 2 as explanatory variables. A total of 4,834 cows (farm A) and 4,238 cows (farm B) in the second lactation were considered for statistical analyses. On farm A, the incidence of PM in lactations 1 and 2 were 20.1% and 11.2%, respectively. On farm B, the incidence of PM in lactations 1 and 2 were 14.4% and 8.5%, respectively. On both farms, cows with PM in their first lactation had greater odds for RFM and PM in their second lactation, whereas there was no association of PM in the first lactation with any other nonuterine diseases (i.e., CH, KET, LDA, and MAST) in the second lactation. Cows with PM in lactation 2 had reduced milk yield. The reduction in milk yield in second lactation was greater for cows that already experienced PM in lactation 1. On farm A, cows with PM in their first lactation had a greater hazard for culling within 60 DIM of the second lactation; however, the same association was not present on farm B. Cows with PM in lactation 1 had reduced pregnancy per AI at first service in the second lactation only on farm B. Cows with PM in lactation 2 had reduced pregnancy per AI at first service in the second lactation on both farms. Pregnancy loss in lactation 2 was only associated with PM in lactation 2 but not with PM in lactation 1. On both farms, cows had a reduced hazard for pregnancy in their second lactation within 250 DIM when they experienced PM in either lactation. In conclusion, PM in the first lactation had long-lasting negative consequences (i.e., risk of uterine disease and lower reproductive performance) for cows in their next lactation.

SYMPOSIUM REVIEW: EXPLORING SUSTAINABILITY IN DAIRY CATTLE BREEDING FOCUSING ON FEED EFFICINCY AND METHANE EMISSIONS

Reducing emissions is vital to improve sustainability and industry leaders have set emission goals to either reduce gross emissions, lower emissions intensity, or reach net-zero. However, additional traits should also be measured and compared in terms of their impact on the broader definition of sustainability. In addition to environmental impact, a sustainable breeding objective must consider profit, animal welfare, farmer well-being, and social responsibility. Traits to be considered include direct emissions (e.g., nitrogen and methane), production efficiency (e.g., feed efficiency), health (e.g., calf and transition cow health) and welfare traits (e.g., polled). Many of these novel traits require labor- and/or cost-intensive phenotyping procedures. Consequently, this results in relatively modest data sets and estimated breeding values with limited reliability. Opportunities exist to overcome this limitation by developing cost-effective and easily quantifiable proxy traits and utilizing international collaboration to expand novel phenotype reference populations. Furthermore, non-economic values can be estimated to quantify the impact a trait has on societal perspective (e.g., farmer preference) or environmental impact (methane emissions), and combined with economic weights to calculate aggregate weights for each trait. While validation techniques are still uncertain, the United Nations Sustainable Development Goals may be applied to determine the improvement in sustainability due to genetic selection. This approach provides the flexibility to accommodate diverse sustainability perspectives, encompassing the disparities between developed and developing countries. Currently, the number and quality of relevant phenotypes are the main limiting factor. However, as confidence grows in the opportunity to improve sustainability through genetic selection, substantial new investment will be required both in refining phenotyping methodologies and conceptualizing novel breeding designs.

Predictive modelling of deviation from expected milk yield in transition cows on automatic milking systems

The transition period is a pivotal time in the production cycle of the dairy cow. It is estimated that between 30% and 50% of all cows experience metabolic or infectious disease during this time. One of the most common and economically consequential effects of disease during the transition period is a reduction in early lactation milk production. This has led to the utilisation of deviation from expected milk yield in early lactation as a proxy measure for transition health. However, to date, this analysis has been used exclusively for the retrospective assessment of transition cow health. Statistical models capable of predicting deviations from expected milk yield may allow producers to proactively manage animals predicted to suffer negative deviations in early lactation milk production. The objective of this retrospective cohort study was first, to explore the accuracy with which cow-level production and behaviour data collected on automatic milking systems (AMS) from 1–3 days in milk (DIM) can predict deviation from expected 30-day cumulative milk yield in multiparous cows. And second, to assess the accuracy with which predicted yield deviations can classify cows into groups which may facilitate improved transition management. Production, rumination, and physical activity data from 31 commercial AMS were accessed. A 3-step analytical procedure was then conducted. In Step 1, expected cumulative yield for 1–30 DIM for each individual cow-lactation was calculated using a mixed effect linear model. In Step 2, 30-Day Yield Deviation (YD) was calculated as the difference between observed and expected cumulative yield. Lactations were then assigned to one of three groups based on their YD, RED Group (</= −15% YD), AMBER Group (−14% ̶ 0% YD), GREEN Group (>0% YD). In Step 3, yield, rumination, and physical activity data from days 1–3 in lactation were used to predict YD using machine learning models. Following external validation, YD was predicted across the test data set with a mean absolute error of 9%. Categorisation of animals suffering large negative deviations (RED group) was achieved with a specificity of 99%, sensitivity of 35%, and balanced accuracy of 67%. Our results suggest that milk yield, rumination and physical activity patterns expressed by dairy cows from 1–3 DIM have utility in the prediction of deviation from expected 30-day cumulative yield. However, these predictions currently lack the sensitivity required to classify cows reliably and completely into groups which may facilitate improved transition cow management.

Association of transition cow health with pregnancy per artificial insemination and pregnancy loss in Holstein cows submitted to a Double-Ovsynch protocol for first service

This observational study was conducted to evaluate the effect of transition cow health on pregnancy per artificial insemination (P/AI) and pregnancy loss (PL) in cows submitted to a Double-Ovsynch protocol (DO) for first service. Lactating Holstein cows (n = 15,041) from one commercial dairy farm in northern Germany between January 2015 to December 2021 were enrolled into a modified Double-Ovsynch protocol (GnRH, 7 d later PGF2α, 3 d later GnRH, 7 d later GnRH, 7 d later PGF2α, 24 h later PGF2α, 32 h later GnRH, and 16 to 18 h later timed artificial insemination) for first service at 72 ± 3 d in milk. Pregnancy was diagnosed at 32 and 60 d post-AI via transrectal ultrasonography. Pregnancy loss was defined as the proportion of cows diagnosed pregnant 32 d post-artificial insemination that were diagnosed nonpregnant 60 d post-artificial insemination. Health-related events (i.e., milk fever [MF], hyperketonemia [KET], retained fetal membranes [RFM], metritis, mastitis, left displaced abomasum [LDA]) were assessed by farm personnel using standard operating procedures. Multivariable logistic regression was used for testing potential associations between transition cow health event occurrence and outcome variables, including P/AI and PL. Three separate models were built for cows in first lactation, second lactation, and ≥third lactation. Overall, 20.0% (885/4,430), 34.9% (1,391/3,989), and 53.9% (3,570/6,622) of cows had at least one transition cow health event for first, second, and ≥third lactations, respectively. The most prevalent transition cow health event for first-lactation cows was metritis (10.7%; [473/4,430]), whereas second-lactation cows suffered mostly from mastitis (16.6%; [664/3,989] and KET (16.6%; [661/3,989]), and cows with ≥third lactations were mostly affected by KET (33.2%; [2,198/6,622]). We observed a negative association between inflammatory disorders (i.e., RFM, metritis, mastitis) and P/AI in all cows irrespective of parity. Metabolic disorders (i.e., MF, KET, LDA) were negatively associated with P/AI only in multiparous cows. Irrespective of parity, only uterine diseases (i.e., RFM, metritis) were significantly associated with PL. These results show that enrolling cows into a fertility protocol, such as DO, cannot overcome the carryover effects of inflammatory and metabolic disorders on P/AI and PL and highlight the importance of optimizing transition cow health as a prerequisite for achieving high fertility in a DO protocol.

Perspective: Can early embryonic losses be reduced in lactating dairy cows?

Perspective: Can early embryonic losses be reduced in lactating dairy cows?

Effect of transition cow health and estrous expression detected by an automated activity monitoring system within 60 days in milk on reproductive performance of lactating Holstein cows

The objective of this observational study was to evaluate the association of transition cow health and estrous expression, detected by an automated activity monitoring system (Smarttag Neck, Nedap Livestock Management), with reproductive performance in lactating Holstein cows. A total of 3,750 lactating Holstein cows (1,563 primiparous cows and 2,187 multiparous cows) from a commercial dairy farm in Slovakia calving from January 2020 until July 2021 were enrolled on an ongoing basis. Activity data were recorded from d 7 until d 60 postpartum. Within this observational period, cows were classified into 3 categories: (1) no estrus event (Estrus0), (2) 1 estrus event (Estrus1), or (3) 2 or more estrus events (Estrus2+). Transition cow health was assessed by farm personnel within the first 30 d in milk (DIM) using standard operating procedures. Generalized linear mixed models were used to analyze continuous and categorical data. Cox proportional hazard models were used for time to event data. The overall prevalence of anestrus was 20.8%. Multiparous cows had a greater risk for anestrus compared with primiparous cows [odds ratio (OR) = 1.4]. Cows with stillbirth (OR = 1.76), retained placenta (OR = 2.19), puerperal metritis (OR = 1.48), or subclinical ketosis (OR = 1.51) had a greater risk for anestrus. In addition, cows calving in summer (OR = 0.82), autumn (OR = 0.38), or winter (OR = 0.56) had a higher incidence of anestrus than cows calving in spring. Estrous expression from d 7 until d 60 postpartum was associated with estrous duration (DU) and estrous intensity at first artificial insemination (AI). Cows in Estrus0 had the shortest DU at first postpartum AI (9.4 ± 0.18 h) compared with cows in Estrus1 (10.5 ± 0.13 h) and Estrus2+ (11.4 ± 0.12 h). Cows in Estrus2+ had a longer DU at first postpartum AI compared with cows in Estrus1. For Estrus0, Estrus1, and Estrus2+ cows, pregnancy per AI at first service was 42.5%, 50.9%, and 55.4%, respectively. Estrous expression from d 7 until d 60 postpartum was associated with time to first AI and time to pregnancy. Compared with Estrus0 cows, Estrus1 [hazard ratio (HR) = 1.43] and Estrus2+ cows (HR = 1.62) had an increased hazard of being inseminated within 100 DIM. Compared with Estrus2+, Estrus1 cows had a reduced hazard of being inseminated within 100 DIM (HR = 0.89). Compared with Estrus0 cows, Estrus1 (HR = 1.24) and Estrus2+ cows (HR = 1.46) had an increased hazard of becoming pregnant within 200 DIM. Median DIM to pregnancy were 121, 96, and 92 for Estrus0, Estrus1, and Estrus2+ cows, respectively. In conclusion, cows with transition cow disorders (i.e., stillbirth, retained placenta, puerperal metritis, or subclinical ketosis) had a greater chance for anestrus compared with healthy cows. Cows in Estrus0 had reduced estrous expression at first AI and inferior reproductive performance compared with cows that displayed estrous activity from d 7 until d 60.

Defining breeding objectives for sustainability in cattle: challenges and opportunities

This paper reviews breeding objectives of the current global cattle industry and outlines existing challenges and opportunities for improving sustainability in the livestock sector through genetic selection. Cattle breeding programs have historically been focused on generating profit by selecting for high-producing animals and broadened to include traits related to health, reproduction, fertility, and efficiency. Now, cattle industries around the world are using genetics to reduce their environmental impacts and improve sustainability. Reducing emissions is vital to improve sustainability, and industry leaders have set emission goals to either reduce gross emissions, lower emissions intensity, or reach net-zero. However, additional traits should also be measured and compared in terms of their impact on the broader definition of sustainability. In addition to environmental impact, a sustainable breeding objective must consider profit, animal welfare, farmer wellbeing, and social responsibility. Traits to be considered include direct emissions (e.g. nitrogen and methane), production efficiency (e.g. feed efficiency, growth), closer to biology reproduction and fertility (e.g. oestrous strength and semen quality), health (e.g. calf and transition cow health) and welfare traits (e.g. polled). Many of these novel traits require labour-intensive or expensive phenotyping, resulting in small datasets and low reliability of estimated breeding values. Opportunities exist to overcome this limitation by utilising international collaboration to build large data bases, develop inexpensive and easy-to-measure proxy traits, and expand novel-phenotype reference populations by using female-driven reference populations and young stock and males. Non-economic values can be estimated that quantify the impact that a trait has on societal perspective (e.g. farmer preference) or the environmental impact (methane emissions), and combined with economic weights to calculate aggregate weights for each trait. While validation techniques are still uncertain, the United Nations Sustainable Development Goals may be applied to determine the improvement in sustainability due to genetic selection. This approach allows for various perspectives of sustainability, such as in the developed versus developing world, to be considered. The number and quality of relevant phenotypes are currently the main limiting factors. As confidence continues to grow in the opportunity to improve sustainability through genetic selection, substantial new investment will be required both in phenotyping activities, but also into novel breeding structures and scheme designs that can maximise the value and impact of these phenotypes.

Temporal profiles describing markers of inflammation and metabolism during the transition period of pasture-based, seasonal-calving dairy cows

The physiology of the dairy cow while transitioning from pregnancy to lactation is complex, with multifactorial processes studied extensively for the role they play in manifestation of disease along with associated economic losses and compromised animal welfare. Manuscripts outlining associations among nutrition, production, physiology, and genetics variables and transition cow disorders are common in literature, with blood analytes that are central to energy metabolism (e.g., nonesterified fatty acids; NEFA, β-hydroxybutyrate; BHB) often reported. Immunity and inflammation have increasingly been explored in the pathogenesis and persistence of disorders, with cytokines and acute phase proteins well documented. However, most of these studies have involved cows fed total mixed rations, which may not always reflect profiles of blood analytes and other physiological indicators of transition cow health in grazing cows consuming fresh pasture. Considering the comparatively lesser characterization of these analytes and markers in pasture-based, seasonal-calving dairy cows, we compiled a database consisting of 2,610 cow lactations that span 20 yr of transition cow research in New Zealand. Using this database, analyte profiles from approximately 28 d precalving to 35 d postcalving were identified in dairy cows with a range of genetics, milk production potentials, and pasture-based farm management systems. These profiles characterize changes in energy reserves and metabolism (NEFA, BHB, glucose, insulin, growth hormone, insulin-like growth factor-1, leptin, body condition score, body weight), liver function (globulin, aspartate aminotransferase, glutamate dehydrogenase, gamma-glutamyl transpeptidase, bilirubin, cholesterol, liver triacylglycerides), protein metabolism (albumin, total protein, albumin:globulin ratio, creatinine, urea, creatine kinase), mineral balance (calcium, magnesium, phosphate, potassium, sodium, chloride, bicarbonate), inflammation (IL-1β, IL-6, haptoglobin, reactive oxygen species, total antioxidant capacity), and uterine health (polymorphonuclear cells, macrophage cells, vaginal discharge score). Temporal changes are generally consistent with previously characterized homeorhetic changes experienced by the dairy cow during the transition from pregnancy to lactation in both pastoral and housed systems. Some of the profiles had not previously been presented for pastoral systems, or in some cases, presented for either system. Our results indicate that moderate-yielding dairy cows undergo similar homeorhetic changes to high-yielding housed cows; however, differences in diet composition result in greater BHB concentrations than expected, based on their milk production and NEFA concentrations. In addition, most cows were able to transition to a state of higher energy requirement following calving, albeit with an increased metabolic challenge in the liver, and only a small percentage of cows were classified with severe hepatic lipidosis or severe hyperketonemia. Increases in metabolic function of the liver were accompanied by changes in indicators of the immune system and changes in mineral balance that, combined, probably reflect the innate response to the transition from gestation to lactation.

Effect of dose rate and timing of administration of pegbovigrastim on white blood cell responses in grazing dairy cows

Transition dairy cows experience a decline in immune function that increases the risk of peripartum disease. One strategy to improve peripartum immune function involves the use of a commercially available cytokine: bovine granulocyte-colony stimulating factor, with the addition of polyethylene glycol to increase duration of effectiveness. Treatment with Imrestor (15 mg pegbovigrastim; Elanco) one week before expected calving date (d -7) and again on the day of calving (d 0) was previously reported to increase the neutrophil number and improve neutrophil function; as a result, the incidence of clinical mastitis was reduced. We conducted 2 experiments over consecutive years to investigate the effect of a lower dose rate (half or quarter dose rate) of Imrestor in grazing dairy cattle and reduced administration frequency: one dose instead of the recommended 2. White blood cell counts were measured to determine changes in relative cell populations in response to treatment. Neutrophil function was assessed by measuring myeloperoxidase activity. Imrestor treatment increased the numbers of neutrophils, band cells, lymphocytes, and monocytes until 14 d postcalving in a dose-dependent manner; it also increased neutrophil myeloperoxidase activity. One dose of Imrestor increased white blood cell counts and myeloperoxidase activity, but the timing, degree, and duration of the response were different relative to the recommended 2 doses and were also dependent upon when Imrestor treatment was given. One dose at d -7 relative to expected calving date did not have a lasting effect postcalving, whereas one dose only on d 0 caused a delayed effect relative to cows that received 2 doses. There was no effect of Imrestor on milk yield or on blood indicators of transition cow health. A lower dose rate of Imrestor or a single dose of Imrestor on the day of calving may be sufficient to improve neutrophil function during the early postpartum in grazing dairy cows. Large-scale field studies are required to determine whether the smaller response from lower dose rates or the timing of the immunological response to drug delivery affect animal health in early lactation.

Why isn’t the transition period getting the attention it deserves? Farm advisors’ opinions and experiences of managing dairy cow health in the transition period

During the transition period three weeks before and after calving the dairy cow is at greater risk of developing disease, to the detriment of welfare and production. An understanding of the reasons why and how farmers and their advisors engage in efforts to control metabolic disease during the transition period is required if these diseases are to be more successfully controlled. The study reported here, based on interview research, investigates the opinions and behaviours of farm advisors on transition cow management and nutrition, their experiences of working with their respective farm clients, and interactions with other farm advisors to help manage transition cow health and productivity. Semi-structured interviews were conducted with 12 veterinary advisors and 12 non-veterinary advisors (nutritionists, feed company representatives and independent consultants) in England. A key theme emerging from this qualitative data was a perceived lack of focussed transition management advice provided by advisors. Reasons for suboptimal or lack of appropriate advice included: time pressures for advisors to visit as many farms as possible; avoiding the investigation of areas of potential improvement, for fear of not meeting transition health and performance targets; financial disincentives for nutritionists, as the sales commission attributed to transition cow feeding was small relative to the main milking herd; and a lack of confidence in the subject. Other aspects included the responsibility of providing transition advice which was perceived to be high-risk, a lack of cooperation between veterinarians and nutritionists, and the perceived varying competencies of nutritionists. The findings demonstrate the importance of the varied influences of ‘people factors’ on transition cow health such as the nature of the advisor-farmer relationship, advisor-farmer communication and herd-level advisor collaboration on transition cow health and management.

High fertility cycle

Over the past 2 decades, a reproduction revolution has occurred in the dairy industry in which average 21-d pregnancy rates have more than doubled from around 14% to more than 30% in many herds. Much of this increase in reproductive performance has been driven by development and adoption of fertility programs. Despite the dramatic increase in 21-d pregnancy rates, substantial variation exists among herds using the exact same reproductive management, suggesting that factors other than fertility programs can affect fertility. Change in body weight or body condition score postpartum or during the periparturient period dramatically affects embryo quality, reproductive outcomes, and transition cow health. Although some cows lose body weight or body condition score after calving, some cows maintain, whereas some cows even gain body weight or body condition score during this time period. Surprisingly, milk production during early lactation is not affected based on body condition score change during the first 3 weeks postpartum; however, peak milk measured near 60 DIM was less in both primiparous and multiparous cows that either gained or maintained compared to cows that lost body condition during the 1st 30 DIM. The high fertility cycle coupled with the dramatic increases in reproductive performance due to the development and adoption of fertility programs is a new paradigm that we can now use to explain much of the variation in reproductive performance among herds. The high-fertility cycle: how timely pregnancies in 1 lactation may lead to less BCS loss, fewer health issues, greater fertility, and reduced early pregnancy losses in the next lactation.

332 Young Scholar Presentation: regulation of immune signaling by extracellular vesicles

Abstract Exosomes and other extracellular vesicles traffic a variety of protein and nucleic acid cargo throughout the body. Immune cells especially utilize these vesicles for communication, and they are being studied in clinical settings for their potential to act as disease markers, but also to serve as drug delivery systems. In animal science, a large part of the research around exosomes has centered around their presence in milk, as well as their bioavailability and potential impact for the neonate and for humans; milk-derived exosomes are enriched with immune-related proteins and microRNA that may relate to maternal health, mammary function, or neonate development. We sequenced miRNA isolated from colostrum of dairy cows predicted to feature either moderate or high degree of immune suppression, but found minimal differences (2 differentially expressed miRNA of 343 miRNA analyzed) between the two groups. Immune suppression exhibited during early lactation may also be related to altered exosome-mediated communication between immune cells. We studied the ability of bovine exosomes to alter immune responses of primary bovine monocyte-derived macrophages and found that exosomes alone or in combination with LPS were able to stimulate cytokine production to a greater extent than LPS alone. The ability of exosomes to transport cytokine, bioactive lipid, and regulatory RNA cargo suggests potential involvement in transition cow health. Altered exosome content and function have been associated with uterine infection, mastitis, and metabolic dysfunction; future studies with exosome signaling may help clarify the complexities of transition cow immune function and point to strategies to support immunity.

Associations of pre- and postpartum lying time with metabolic, inflammation, and health status of lactating dairy cows

The objective was to evaluate the associations of pre- and postpartum lying time (LT) with serum total calcium (Ca), nonesterified fatty acids (NEFA), β-hydroxybutyrate (BHB), and haptoglobin concentrations, hemogram, and health status of dairy cows. A total of 1,052 Holstein cattle (401 nulliparous heifers and 651 parous cows) from 3 commercial dairy farms were fitted with electronic data loggers (IceQube, IceRobotics, Edinburgh, UK) on a hind leg 14 ± 3 d before parturition (dpp) and removed at 14 ± 3 d in milk (DIM) to assess their LT. Lying time data were summarized and reported daily (min/d or h/d). Serum concentrations of NEFA (at 14 ± 3 and 7 ± 3 dpp), total serum calcium within 48 h after calving, and BHB (at 7 ± 3 and 14 ± 3 DIM) were determined. Serum concentration of haptoglobin was determined and a hemogram was performed on a subsample of 577 cows (237 primiparous and 340 multiparous) at 7 ± 3 DIM. Cases of milk fever, retained placenta, metritis, mastitis, pneumonia, and digestive disorders within 30 DIM were recorded and cows were categorized into 1 of 4 groups: (1) nondiseased (ND, n = 613; cows without ketosis and any other health conditions); (2) cows with only ketosis (KET, n = 152); (3) sick cows experiencing ≥1 health conditions, but without ketosis (SICK, n = 198); or (4) cows with ketosis plus at least one other health condition (KET+, n = 61). Data were analyzed using mixed linear regression models or logistic regression (MIXED or GLIMMIX procedures). Lying time within 14 dpp had a significant positive quadratic association with serum NEFA concentrations at 14 ± 3 and 7 ± 3 dpp but was not significantly associated with serum Ca concentration within 48 h after calving. Lying time during the first 14 DIM after parturition had a significant linear association with the risk of ketosis within 14 DIM. For every 1-h increment in mean LT (from 8 to 15 h/d) within the first 14 DIM after calving, the risk of diagnosis with ketosis within 14 DIM increased by 3.7 percentage points. Regardless of parity, a greater proportion of KET and KET+ groups had increased serum prepartum NEFA concentration (≥400 µEq/L) and increased body condition loss from 14 dpp to 28 DIM compared with SICK and ND cows. A greater proportion of multiparous KET and KET+ cows had hypocalcemia within 48 h after calving compared with ND and SICK cows, but we did not detect a significant association between hypocalcemia and health status on primiparous cows. Multiparous KET+ cows had significantly reduced neutrophils and white blood cell count compared with ND cows, but lymphocytes did not differ. Regardless of parity, KET+ and SICK cows had significantly higher concentrations of serum haptoglobin compared with ND cows. These results suggest that LT along with energy and Ca balance are critical for transition cow health.

Case report

150-cow Holstein dairy herd was experiencing a higher than desirable incidence of displaced abomasum (8% all lactations), ketosis (60% lactation 1, 75% lactation 2 and greater), and metritis (70% lactation 1, 30% lactation 2 and greater) in transition cows. The producer initially refused to implement recommended management changes that would have potentially improved transition cow health of the herd. The increased incidence of transition cow disease, along with the producer’s reluctance to make management changes, led the nutritionist and the veterinarian to partner to utilize The Vital 90™ Days risk assessment tool (dRisk) and economic assessment tool (dEconPro) as a way of identifying and motivating management changes. The results from the dEconPro showed a pre-management (1 year prior = group PRE) to post-management (1 year after = group POST) reduction in total cost per calving of $269 due to decreased incidence of ketosis, metritis, and displaced abomasum. There was significant parity-by-group interaction for milk production, with first-lactation POST cows producing more first-test milk than PRE (68.1 vs 63.5 lb [30.9 vs 28.8 kg], P=0.05). There was a tendency for more peak milk and more total 305ME milk for POST vs PRE (84.6 vs 81.0 lb [38.4 vs 36.7 kg], P=0.13 and 25,995 vs 24,953 lb [11,791 vs 11,319 kg], P=0.10 respectively). There was no difference in multiparous cows, and no difference in removals by 60 or 400 DIM for all cows. For reproductive performance, there was a tendency for higher odds of successful first service for POST vs PRE (1.83, P=0.14), but no difference in time to pregnancy through 200 DIM. The results show that The Vital 90 Days dEconPro and dRisk proved to be extremely valuable tools to help motivate positive change on this dairy. The main objective of this case study was to demonstrate how the team approach on a dairy farm could combine with the use of transition cow risk- assessment tools to validate management recommendations and quantify the impact of management changes.

Methods of measurement and implications of abnormal calcium concentrations in fresh dairy cows

Hypocalcemia is a prevalent disorder in the early postpartum period. Advances in periparturient nutrition and management strategies have decreased the occurrence of clinical hypocalcemia over the past few decades; however, subclinical hypocalcemia remains an important aspect of transition cow health. These proceedings will discuss the diagnostic tools available to bovine practitioners for individual animal calcium concentration determination and herd-level monitoring, as well as current developments in the epidemiology of hypocalcemia and its prevention.

Updates in transition cow health and nutrition

Transition cow nutritional management strategies have evolved significantly over the past 10 years and continue to evolve. Management of subclinical hypocalcemia is important to support high milk yield, excellent reproduction, and decrease subclinical disease. Feeding anionic diets before calving, perhaps using more aggressive strategies, improves blood calcium status post-calving and increases post-calving dry matter intakes and milk yield. Controlling energy intake before calving improves postpartum energy balance and decreases subclinical ketosis; feeding adequate metabolizable protein before calving helps maintain high milk yield after calving. Excellent feeding management (managing chop length of bulky forage and moisture of the (total mixed ration) TMR) of transition rations is critical for on-farm success; many dairy farms have opportunities for improved feeding management. There may be interactions of starch levels pre- and post-calving; cows fed low starch pre-calving rations should be fed lower starch diets after calving, whereas cows fed moderate starch rations pre-calving can likely be fed starch levels more typical of high starch cow rations after calving. Furthermore, there may be opportunities to combine higher fermentability with higher bulk in fresh cow rations to improve feed intakes after calving, as well as to more critically consider metabolizable protein and amino acid formulation of the fresh diet.

Impact of immunosuppression during the periparturient period in dairy cattle

The majority of sick dairy cows that require treatment by farm staff or veterinary practitioners are seen during early lactation, and are typically labelled as ‘transition cow health problems’. Milk fevers, retained fetal membranes, metritis, displaced abomasum, ketosis and mastitis are all conditions that classically occur at this critical time, and it is widely acknowledged that the transition period (defined as the period from 3 weeks pre-calving to 3 weeks after calving) is a high-risk time in the production cycle of the cow. What happens during this critical period can have long-term implications for cow health, longevity, productivity and future fertility.

Benchmarking Reproductive Efficiency and Transition Cow Health of Kansas Dairy Herds

Benchmarking Reproductive Efficiency and Transition Cow Health of Kansas Dairy Herds

Effects of direct-fed Bacillus pumilus 8G-134 on feed intake, milk yield, milk composition, feed conversion, and health condition of pre- and postpartum Holstein cows

The usage of direct-fed microbials (DFM) has become common in the dairy industry, but questions regarding choice of strain, mode of action, and efficacy remain prevalent. The objective of this study was to evaluate the effects of a DFM (Bacillus pumilus 8G-134) on pre- and postpartum performance and incidence of subclinical ketosis in early lactation. Forty-three multiparous Holstein cows were assigned to 2 treatments in a randomized complete block design; cows in the direct-fed microbial treatment (DFMt, n=21) received 5.0×109 cfu/cow of B. pumilus in 28 g of a maltodextrin carrier, whereas cows in the control treatment (CON, n=22) received 28 g of maltodextrin carrier alone. Treatments were top-dressed on the total mixed ration daily. Treatments were applied from 21 d before expected calving date to 154 d after calving. Cows on treatment DFMt tended to have lower serum haptoglobin concentration than CON cows on d 14. Cows on treatment DFMt had higher IgA concentrations in milk than CON cows during the first week after calving. Cows fed DFMt had higher yields of milk, fat-corrected milk, energy-corrected milk, milk fat, and milk protein during the second week of lactation than CON; however, we found no differences between treatments on milk yield and milk components overall. Cows on DFMt tended to have higher feed conversion and to have lower prevalence of subclinical ketosis (beta-hydroxybutyrate >1.2 mmol/L) on d 5 than cows fed CON. Dry matter intake, body weight, and body condition score were not affected by DFMt supplementation. Milk production efficiencies (calculated based on fat-corrected milk and energy-corrected milk) were higher by 0.1 kg of milk per kilogram of dry matter intake in cows that received DFMt compared with cows that received CON. In conclusion, cows receiving DFMt tended to have lower incidence of subclinical ketosis than cows receiving CON. Cows fed DFMt tended to have higher feed conversion and evidence for greater immunity than CON. Supplementation with B pumilus 8G-134 may provide benefits for transition cow health and milk production efficiency.

Lipid mobilization assessment in transition dairy cattle using ultrasound image biomarkers

Excessive lipid mobilization during the transition period of dairy cows predisposes animals to higher disease incidence and reduces lactation performance. Plasma non-esterified fatty acids (NEFA) are used as a marker of lipid mobilization intensity to monitor transition cow management and as a disease risk predictor. NEFA evaluation can be complemented by continuous monitoring of adipose tissue depth reductions during the transition period using ultrasound images of the retroperitoneal (RPAT) and subcutaneous adipose tissue (BFAT) depots. These image biomarkers are easily obtainable and their real time nature offers an important advantage that could help improve transition cow health programs. The objective of this study was to evaluate the use of both RPAT and BFAT ultrasound measurements as quantitative image biomarkers and its association with plasma NEFA. A longitudinal cohort study evaluated adipose image biomarkers in 44 Holstein cows in a commercial dairy herd. Ultrasound examination and blood samples collection were performed at 4 (dry) and 2–3 (close-up) weeks before expected calving date, and at 1 (calving) and 4 (lactation) weeks after parturition. Both RPAT and BFAT depth measurements were significantly lower at parturition and at the lactation samples compared to pre-calving measures and were negatively associated with plasma NEFA. Only BFAT predicted minimum concentrations of plasma NEFA before and after calving. The use BFAT as an image biomarker of lipid mobilization and its risk predictive value in other herds with different management styles requires further investigation.