Transient Temperature Increase Research Articles

HSP70—A key regulator in chondrocyte homeostasis under naturally coupled hydrostatic pressure-thermal stimuli

ObjectiveDuring physical activities, chondrocytes experience coupled stimulation of hydrostatic pressure (HP) and a transient increase in temperature (T), with the latter varying within a physiological range from 32.5 °C to 38.7 °C. Previous short-term in vitro studies have demonstrated that the combined HP-T stimuli more significantly enhance chondroinduction and chondroprotection of chondrocytes than isolated applications. Interestingly, this combined benefit is associated with a corresponding increase in HSP70 levels when HP and T are combined. The current study therefore explored the indispensable role of HSP70 in mediating the combined effects of HP-T stimuli on chondrocytes. DesignIn this mid-long-term study of in vitro engineered cartilage constructs, we assessed chondrocyte responses to HP-T stimuli using customized bioreactor in standard and HSP70-inhibited cultures. ResultsSurprisingly, under HSP70-inhibited conditions, the usually beneficial HP-T stimuli, especially its thermal component, exerted detrimental effects on chondrocyte homeostasis, showing a distinct and unfavorable shift in gene and protein expression patterns compared to non-HSP70-inhibited settings. Such effects were corroborated through mechanical testing and confirmed using a secondary cell source. A proteomic-based mechanistic analysis revealed a disruption in the balance between biosynthesis and fundamental cellular structural components in HSP70-inhibited conditions under HP-T stimuli. ConclusionsOur results highlight the critical role of sufficient HSP70 induction in mediating the beneficial effects of coupled HP-T stimulation on chondrocytes. These findings help pave the way for new therapeutic approaches to enhance physiotherapy outcomes and potentially shed light on the elusive mechanisms underlying the onset of cartilage degeneration, a long-standing enigma in orthopedics.

Testing S. sonnei GMMA with and without Aluminium Salt-Based Adjuvants in Animal Models.

Shigellosis is one of the leading causes of diarrheal disease in low- and middle-income countries, particularly in young children, and is more often associated with antimicrobial resistance. Therefore, a preventive vaccine against shigellosis is an urgent medical need. We have proposed Generalised Modules for Membrane Antigens (GMMA) as an innovative delivery system for Shigella sonnei O-antigen, and an Alhydrogel formulation (1790GAHB) has been extensively tested in preclinical and clinical studies. Alhydrogel has been used as an adsorbent agent with the main purpose of reducing potential GMMA systemic reactogenicity. However, the immunogenicity and systemic reactogenicity of this GMMA-based vaccine formulated with or without Alhydrogel have never been compared. In this work, we investigated the potential adjuvant effect of aluminium salt-based adjuvants (Alhydrogel and AS37) on S. sonnei GMMA immunogenicity in mice and rabbits, and we found that S. sonnei GMMA alone resulted to be strongly immunogenic. The addition of neither Alhydrogel nor AS37 improved the magnitude or the functionality of vaccine-elicited antibodies. Interestingly, rabbits injected with either S. sonnei GMMA adsorbed on Alhydrogel or S. sonnei GMMA alone showed a limited and transient body temperature increase, returning to baseline values within 24 h after each vaccination. Overall, immunisation with unadsorbed GMMA did not raise any concern for animal health. We believe that these data support the clinical testing of GMMA formulated without Alhydrogel, which would allow for further simplification of GMMA-based vaccine manufacturing.

Ultrafast All‐Optical Control of Light Chirality with Nanostructured Graphene

AbstractUltrafast nanophotonics is a rapidly growing area of study focused on creating nanodevices that can modulate the properties of light at, to this date, unparalleled speed. To facilitate the growth of this field, there is a growing need for compact metamaterial designs for the manipulation of the amplitude, phase, and polarization of light. One promising strategy involves leveraging the optical nonlinearity of nanostructured materials to alter their permittivity by interacting with high‐intensity ultrashort laser pulses. This study showcases how such requirements can be met through the utilization of 2D materials, particularly graphene. The nonlinear optical response of a graphene nanorectangle array is theoretically modeled to achieve all‐optical, fully reversible, broadband, and ultrafast dynamic control of light chirality. This is achieved by taking advantage of the energy relaxation dynamics of coherently excited localized plasmons supported by the metasurface, and the transient increase in electron temperature in graphene. Using finite‐difference time‐domain simulations, ultrafast dynamic tuning between circular and linearly polarized light is demonstrated. The proposed platform gives promise for ultrathin, CMOS‐compatible nanophotonic systems that can provide high‐speed, room‐temperature modulation of light polarization.

Experimental and numerical analysis of the effects of thermal degradation on carbon monoxide oxidation characteristics of a three-way catalyst

This work investigates oxygen-storage capacity (OSC) changes during thermal degradation in modern three-way catalysts. Two experiments are performed using catalysts with different degradation degrees to evaluate OSC and reaction rates. The CO2 production test, where CO and O2 are supplied at a constant temperature, shows decreased CO2 production with more degraded catalysts and reduced purification. The CO2 production test is conducted using transient temperature increases, showing that the maximum CO2 production temperature increases with catalyst degradation. The results reveal an increase in activation energy in the oxygen desorption reaction caused by thermal degradation progresses and a decrease in OSC, resulting in temperature increases in the oxygen storage reaction. In the surface reaction and mass transport model considering the 30 elementary reactions, the predicted results are well-validated for CO2 production, enabling good oxygen storage predictions based on actual data. These results can be used to predict OSC by formulating the changes in active site density and activation energy due to degradation.

The effect of strain on the crystallinity of carbonaceous matter: Application of Raman spectroscopy to deformation experiments

Raman spectroscopy, applied to the carbonaceous matter (CM) present in fault zones, is a potentially powerful tool to aid in the reconstruction of slip conditions, provided the respective effect of heating and strain can be disentangled. For this purpose, we have carried out static heating and deformation experiments on low-grade sediments containing disseminated CM particles and tracked the evolution of CM crystalline ordering (crystallinity) using Raman spectroscopy. Heating at high pressure (200 MPa), at varying temperatures (500 to 800 °C) and durations (3 to 1080 h) resulted in an increased crystallinity, which was observed by an increase in the intensity ratio of the Disordered Band over the Graphite Band (Intensity Ratio R1 parameter) of the Raman spectra. The experimental relationship between R1, T and duration was compiled into a law of evolution, which was then used to design the deformation experiments. Experiments were performed in a Paterson rig at 150 MPa or in a Griggs-type rig at 1 GPa. We observed two types of deformation microstructures: (1) purely brittle ones, such as breccia along slip planes and (2) ductile ones, such as foliated layers, resulting from grain reorientation and comminution along with dissolution precipitation. In all deformation microstructures, an increase in R1 is visible and interpreted to be the result of higher crystallinity of carbonaceous particles. Such increase is negligible in breccia, but is significant, up to 40%, in ductile deformation zones. The slow strain rates prevailing in ductile deformation prevented any local temperature increase, therefore the increase in crystallinity of the CM can be solely attributed to deformation. Thus, in natural fault zone, the increased crystallinity of CM reported in the literature and interpreted to be the result of frictional heating during earthquakes might rather be the result of strain localization without transient temperature increase.

Epoxy composites based on phase change microcapsules with high thermal conductivity and storage efficiency by dispersing with cellulose nanofibrils

The incorporation of microencapsulated phase change materials (MPCMs) into electronic packaging materials has demonstrated the potential to reduce the transient increase in temperature of electronic devices under thermal loading. However, because of their high specific surface area, MPCMs tend to form severe aggregates in polymer matrices, resulting in poor thermal conductivity and energy storage efficiency. Therefore, in this paper, cellulose nanofibers (CNF) were used as dispersants to effectively disperse MPCMs in the epoxy resin matrix to synthesize epoxy composites with superior thermal conductivity and thermal storage properties (referred to as EBM-C). The results demonstrated that the addition of CNF improved the dispersion of MPCMs as well as the thermal conductivity and thermal storage capacity of the composites. The thermal conductivity and energy storage efficiency of the composites with 3 wt% CNF were increased by 100 %, and 97 %, respectively, compared to those of composites without CNF. In addition, the EBM-C composites also had excellent thermal stability and mechanical properties. The experimental results conclusively demonstrate that the EBM-C composites have tremendous application potential as thermal management materials.

Importance, influence and limits of CFD radiation modeling for containment atmosphere simulations

In the appearance of steam, the CFD modeling of thermal-hydraulic phenomena in reactor containments during various phases of an accident necessarily requires consideration of radiative heat transfer. These radiation phenomena involve (i) energy transfer within the gas mixture and (ii) between the gas and the surrounding structures which have a much higher thermal inertia. Preliminary calculations performed for the present experiments in the OECD/NEA HYMERES-2 project with the CFD code containmentFoam using a Monte Carlo thermal radiation solver with non-gray gas modeling of steam absorption, showed that radiant heat transfer is important even with a very low amount of steam (≈ 2%). Therefore, the test matrix was tailored to the two opposite extremes: Either gas compositions with low steam content, where radiative heat transfer can be neglected, or gas mixtures containing larger amounts of steam, so that radiative heat transfer plays a dominant role. For the two selected experiments within the H2P2 series and the corresponding CFD calculations, a vessel with a diameter of 4 m and a height of 8 m was preconditioned with different mixtures of air/steam at room and elevated temperatures. This was followed by the build-up of a stable helium stratification in the upper part of the vessel. Helium was then injected from the top at a higher mass flow rate, which (a) compresses the gas atmosphere, (b) resembles piston compression and (c) leads to a height-dependent and transient increase in gas temperature below this helium stratification, influenced by thermal radiation effects — or their absence. These experiments and the associated CFD calculations were developed to isolate the thermal radiation phenomena as much as possible from convective and diffusive effects. They are the first of their kind to be conducted in a large-scale thermal-hydraulic facility. It is demonstrated (a) that neglecting thermal radiation for the high steam content case, the temperature rise is significantly over-predicted by a factor of two compared to the application of the suggested Monte Carlo thermal radiation solver with non-gray gas modeling, (b) the computational effort considering CFD and non-gray gas radiation is feasible with the suggested tailored Monte Carlo solver and (c) that the suggested radiation model outperforms the widespread used P1 radiation model.

Natural history of changes in knee skin temperature following total knee arthroplasty: a systematic review and meta-analysis

Patients undergoing total-knee arthroplasty (TKA) have transient increases in anterior knee skin temperature (ST) that subside as recovery progresses–except in cases of systemic or local prosthetic joint infections (PJI). This meta-analysis was designed to quantify the changes in knee ST following TKA in patients with uncomplicated recovery as a prerequisite for assessing the usefulness of thermal imaging for diagnosis of PJI. This meta-analysis (PROSPERO-CRD42021269864) was performed according to PRISMA guidelines. PUBMED and EMBASE were searched for studies reporting knee ST of patients that underwent unilateral TKA with uncomplicated recovery. The primary outcome was the weighted means of the differences in ST between the operated and the non-operated knees (ΔST) for each time point (before TKA, and 1 day; 1,2, and 6 weeks; and 3,6, and 12-months post-TKA). For this analysis, 318 patients were included from 10 studies. The elevation in ST was greatest during the first 2-weeks (ΔST = 2.8 °C) and remained higher than pre-surgery levels at 4–6 weeks. At 3-months, ΔST was 1.4 °C. It decreased to 0.9 °C and 0.6 °C at 6 and 12-months respectively. Establishing the baseline profile of knee ST following TKA provides the necessary first step for evaluating the usefulness of thermography for the diagnosis of post-procedural PJI.

Hyperthermia Using Magnetic Cobalt Ferrite Magnetoelectric Nanoparticles

The focus of this article is to understand the application and the factors influencing magnetic hyperthermia using cobalt ferrite nanoparticles. Intrinsic properties, such as particle size, specific absorption rate, and magnetic saturation, are correlated with the material’s property to generate hyperthermia. External factors, such as the magnitude of applied field frequency, also influence the rate of hyperthermia generated by magnetic nanoparticles. Quantitative analysis of Pennes bioheat equation and finite element analysis (FEA) using COMSOL are used to understand the transient increase in temperature at the tumor site using magnetic nanoparticles. The required temperature for generating hyperthermia using nanoparticles is approximately 315 K or 42 °C. Due to the intrinsic high rate of metabolism in tumor cells, the required magnetic field to induce transient increase at the malignant cell site is less than its surrounding healthier cells. This principle can therefore be applied for inducing selective hyperthermia in tumor cells by the application of a lower threshold external magnetic field. This article focuses on the Pennes bioheat equation to understand the phenomenon of heat transfer as a function of both the time and the applied field magnitude. It compares the difference in the rate of heat transfer for 1) cobalt ferrite and cobalt ferrite–barium titanate and 2) hyperthermia in healthier and tumor sites using magnetic nanoparticles. An FEA is performed in conjunction to understand the rate of temperature profile change occurring at a simulated cell site.

Study on Dynamic Injection Prediction Model of High-Pressure Common Rail Injector under Thermal Effect

This study investigates a prediction model for the cycle injection quantity in a high-pressure common rail injector under a transient thermal boundary. The results show that the transient temperature increase curve calculated by the mathematical model of the common rail injector under adiabatic flow is significantly different from the experimental data. A non-isothermal model of the injector coupled with heat transfer is established, which considers the actual heat transfer phenomenon. The excellent agreement between the new calculation results and the experimental data confirms that the fuel injection process of a common rail injector comprises the coupled phenomena of fuel heating and heat transfer. Based on the established simulation model, it is found that in the continuous injection process of the injector, owing to the thermal effect of injection, the cycle injection quantity decreases gradually with an increase in the injector working time and then stabilizes. Under the condition of an injection pulse width of 1.2 ms and frequency of 100 Hz, when the injection pressure increases from 140 MPa to 300 MPa, the reduction in the cycle injection quantity increases from 3.9% to 7.8%, because the higher injection pressure results in higher transient heat at the nozzle holes. In the work of common rail injector assemblies, to achieve more accurate control of the cycle injection quantity, it is necessary to include the correction of a decreasing cycle injection quantity caused by transient heat in the electronic control system.

Genome sequence and experimental infection of calves with bovine gammaherpesvirus 4 (BoHV-4).

Bovine gammaherpesvirus 4 (BoHV-4) is ubiquitous in cattle worldwide, and it has been detected in animals exhibiting broad clinical presentations. The virus has been detected in the United States since the 1970s; however, its clinical relevance remains unknown. Here, we determined the complete genome sequences of two contemporary BoHV-4 isolates obtained from respiratory (SD16-38) or reproductive(SD16-49) tract specimens and assessed clinical, virological, and pathological outcomes upon intranasal (IN) inoculation of calves with the respiratory BoHV-4 isolate SD16-38. A slight and transient increase in body temperature was observed in BoHV-4-inoculated calves. Additionally, transient viremia and virus shedding in nasal secretions were observed in all inoculated calves. BoHV-4 DNA was detected by nested PCR in the tonsil and regional lymph nodes (LNs) of calves euthanized on day 5 post-inoculation (pi) and in the lungs of calves euthanized on day 10 pi. Calves euthanized on day 35 pi harbored BoHV-4 DNA in the respiratory tract (turbinates, trachea, lungs), regional lymphoid tissues, and trigeminal ganglia. Interestingly, in situ hybridization revealed the presence of BoHV-4 DNA in nerve bundles surrounding the trigeminal ganglia and retropharyngeal lymph nodes (day 35 pi). No histological changes were observed in the respiratory tract (turbinate, trachea, and lung), lymphoid tissues (tonsil, LNs, thymus, and spleen), or central nervous tissues (olfactory bulb and trigeminal ganglia) sampled throughout the animal studies (days 5, 10, and 35 pi). This study contributes to the understanding of the infection dynamics and tissue distribution of BoHV-4 following IN infection in calves. These results suggest that BoHV-4 SD16-38 used in our study has low pathogenicity in calves upon intranasal inoculation.

전기자동차용 구동모터 오일 냉각 순시해석

Typical traction motors of electric vehicles utilize water jacket-based or air-based cooling. Currently, lightweight traction motors are preferred to extend the range of electric vehicles. As a result, direct cooling techniques have garnered significant attention from researchers. Direct coil-based cooling methods are of two types: oil injection type and oil spraying type. Direct oil-based cooling methods exhibit high cooling capacities. In this study, we investigate the transient increments in temperature in 160 KW traction motors. To this end, the power losses of motors were estimated using JMAG software . Using two-phase computational fluid dynamics (CFD) simulation over a duration of 18 s, the transient increase in temperature was predicted . The CFD predictions agreed well with the experimental observations, with an error less than 5%. We expect that the conclusions of this study will contribute to the improvement of the cooling performance of 160 KW traction motors.

Maternal Immune Activation during Pregnancy Alters Postnatal Brain Growth and Cognitive Development in Nonhuman Primate Offspring.

Human epidemiological studies implicate exposure to infection during gestation in the etiology of neurodevelopmental disorders. Animal models of maternal immune activation (MIA) have identified the maternal immune response as the critical link between maternal infection and aberrant offspring brain and behavior development. Here we evaluate neurodevelopment of male rhesus monkeys (Macaca mulatta) born to MIA-treated dams (n = 14) injected with a modified form of the viral mimic polyinosinic:polycytidylic acid at the end of the first trimester. Control dams received saline injections at the same gestational time points (n = 10) or were untreated (n = 4). MIA-treated dams exhibited a strong immune response as indexed by transient increases in sickness behavior, temperature, and inflammatory cytokines. Although offspring born to control or MIA-treated dams did not differ on measures of physical growth and early developmental milestones, the MIA-treated animals exhibited subtle changes in cognitive development and deviated from species-typical brain growth trajectories. Longitudinal MRI revealed significant gray matter volume reductions in the prefrontal and frontal cortices of MIA-treated offspring at 6 months that persisted through the final time point at 45 months along with smaller frontal white matter volumes in MIA-treated animals at 36 and 45 months. These findings provide the first evidence of early postnatal changes in brain development in MIA-exposed nonhuman primates and establish a translationally relevant model system to explore the neurodevelopmental trajectory of risk associated with prenatal immune challenge from birth through late adolescence.SIGNIFICANCE STATEMENT Women exposed to infection during pregnancy have an increased risk of giving birth to a child who will later be diagnosed with a neurodevelopmental disorder. Preclinical maternal immune activation (MIA) models have demonstrated that the effects of maternal infection on fetal brain development are mediated by maternal immune response. Since the majority of MIA models are conducted in rodents, the nonhuman primate provides a unique system to evaluate the MIA hypothesis in a species closely related to humans. Here we report the first longitudinal study conducted in a nonhuman primate MIA model. MIA-exposed offspring demonstrate subtle changes in cognitive development paired with marked reductions in frontal gray and white matter, further supporting the association between prenatal immune challenge and alterations in offspring neurodevelopment.

Two Phase CFD Simulations in Stagnant Water Pools: Unsteady Temperature and Level Variation

In the present work, CFD simulations of transient temperature increase in a pool of water due to indirect contact heating in the middle of the pool has been presented. CFD simulations have been able to mimic the transient phenomena of increase in temperature throughout the pool eventually leading to evaporation of water at the top surface and decrease in the level of the pool due to thermal stratification after several hours of operation. The CFD model is first validated with experimental temperature and water vapour volume fraction profiles at a particular level of the pool from the literature. Predictions show good agreement of less than 10% variation is observed. Spatial temperature profiles for different times are analysed to understand the pool boiling in such pools. The profiles indicate thermal stratification after 10000 seconds. Further, analysis transient variation of stratification parameter confirms the strong thermal stratification after 10000 seconds. The evaporation rate after 10000 seconds from top surface have been measured and compared with empirical models from liter

Electron-Phonon Coupling Parameter of Ferromagnetic Metal Fe and Co.

The electron-phonon coupling (g) parameter plays a critical role in the ultrafast transport of heat, charge, and spin in metallic materials. However, the exact determination of the g parameter is challenging because of the complicated process during the non-equilibrium state. In this study, we investigate the g parameters of ferromagnetic 3d transition metal (FM) layers, Fe and Co, using time-domain thermoreflectance. We measure a transient increase in temperature of Au in an FM/Au bilayer; the Au layer efficiently detects the strong heat flow during the non-equilibrium between electrons and phonons in FM. The g parameter of the FM is determined by analyzing the temperature dynamics using thermal circuit modeling. The determined g values are 8.8–9.4 × 1017 W m−3 K−1 for Fe and 9.6–12.2 × 1017 W m−3 K−1 for Co. Our results demonstrate that all 3d transition FMs have a similar g value, in the order of 1018 W m−3 K−1.

Heat-Shock Induces Granule Cell Dispersion and Microgliosis in Hippocampal Slice Cultures.

Granule cell dispersion (GCD) has been found in the dentate gyrus (dg) of patients with temporal lobe epilepsy (TLE) and a history of febrile seizures but was also recently observed in pediatric patients that did not suffer from epilepsy. This indicates that GCD might not always be disease related, but instead could reflect normal morphological variation. Thus, distribution of newborn granule cells within the hilar region is part of normal dg development at early stages but could be misinterpreted as pathological GCD. In turn, pathological GCD may be caused, for example, by genetic mutations, such as the reeler mutation. GCD in the reeler mutant goes along with an increased susceptibility to epileptiform activity. Pathological GCD in combination with epilepsy is caused by experimental administration of the glutamate receptor agonist kainic acid in rodents. In consequence, the interpretation of GCD and the role of febrile seizures remain controversial. Here, we asked whether febrile temperatures alone might be sufficient to trigger GCD and used hippocampal slice cultures as in vitro model to analyze the effect of a transient temperature increase on the dg morphology. We found that a heat-shock of 41°C for 6 h was sufficient to induce GCD and degeneration of a fraction of granule cells. Both of these factors, broadening of the granule cell layer (gcl) and increased neuronal cell death within the gcl, contributed to the development of a significantly reduced packaging density of granule cells. In contrast, Reelin expressing Cajal–Retzius (CR) cells in the molecular layer were heat-shock resistant. Thus, their number was not reduced, and we did not detect degenerating CR cells after heat-shock, implying that GCD was not caused by the loss of CR cells. Importantly, the heat-shock-induced deterioration of dg morphology was accompanied by a massive microgliosis, reflecting a robust heat-shock-induced immune response. In contrast, in the study that reported on GCD as a non-specific finding in pediatric patients, no microglia reaction was observed. Thus, our findings underpin the importance of microglia as a marker to distinguish pathological GCD from normal morphological variation.

Neural Stimulation In Vitro and In Vivo by Photoacoustic Nanotransducers

Summary Neuromodulation is an invaluable approach for the study of neural circuits and clinical treatment of neurological diseases. Here, we report semiconducting polymer nanoparticles based photoacoustic nanotransducers (PANs) for neural stimulation in vitro and in vivo. Our PANs strongly absorb the nanosecond pulsed laser in the near-infrared second window (NIR-II) and generate localized acoustic waves. PANs are shown to be surface modified and selectively bind onto neurons. PAN-mediated activation of primary neurons in vitro is achieved with ten 3-ns laser pulses at 1,030 nm over a 3-ms duration. In vivo neural modulation of mouse brain activities and motor activities is demonstrated by PANs directly injected into brain cortex. With submillimeter spatial resolution and negligible heat deposition, PAN stimulation is a new non-genetic method for precise control of neuronal activities, opening up potentials in non-invasive brain modulation.

Comparison of different analytical methods to evaluate the heat shock protein (HSP) response in fruits. Application to tomatoes subjected to stress treatments

Heat shock proteins (HSP) are synthesized in living tissues exposed to transient increase in temperature and play a central role in the protective response against heat and other stresses. In fruits, this response to heat treatment provides resistance to a physiological alteration known as chilling injury. Despite the physiological importance of this group of proteins, publications comparing different methodological alternatives for their analysis are rather scarce. In the present paper, we conducted a comparative study using different electrophoretic and immunological techniques to evaluate the HSP response in fruits. Proteins were extracted from tomato fruit exposed to an HSP-inducing temperature (38 °C) for different times (0, 3, 20, and 27 h). Different alternatives of analysis (SDS-PAGE, SDS-PAGE followed by IEF, Western blot, and dot blot) were performed, and their potential application discussed. The study was complemented with a practical application, in which tomatoes were subjected to heat and anaerobic treatments and then stored in a chill-inducing temperature. This application evidences the relevance of knowing the level of proteins attained by stress treatments which correlates with the acquired tolerance.

Influence of undulating wall heat and mass flux on MHD natural convection boundary layer flow from a vertical wall

AbstractCurrent study expounds an unsteady magnetohydrodynamic natural convective flow along a vertical wall in presence of variable transverse magnetic field. Small amplitude undulation in wall heat flux and wall mass flux are imposed at the vertical wall to generate the boundary layer flow. The flow governing equations are divided into sets of steady and unsteady equations and then transformed into the similarity and nonsimilarity equations, respectively, by introducing stream function formulations. The sets of nonsimilarity equations are solved numerically by using three different techniques, namely, perturbation solution technique, asymptotic solution technique and implicit finite difference technique applied, respectively, for lower, higher, and all frequencies (ξ). Results are illustrated in connection with the amplitude and phase angles of shear stress, wall temperature, and concentration against the frequency (ξ) for wide ranges of physically significant parameters. Likening of the results obtained by above mentioned numerical methods are presented in every figure and table. Results reveal that the amplitude of undulating shear stress and wall temperature dwindle and the amplitude of wall concentration increases due to increment in Prandtl number (Pr). Besides, on incrementing Schmidt number (Sc) the amplitude of undulating shear stress and wall concentration dwindle and the amplitude of wall temperature increases. Results also reveal that on incrementing magnetic parameter (M) the amplitude of transient shear stress dwindles while the amplitude of transient wall temperature and concentration increase.

Algorithmically Controlled Electroporation: A Technique for Closed Loop Temperature Regulated Pulsed Electric Field Cancer Ablation.

To evaluate the effect of a closed-loop temperature based feedback algorithm on ablative outcomes for pulsed electric field treatments. A 3D tumor model of glioblastoma was used to assess the impact of 2μs duration bipolar waveforms on viability following exposure to open and closed-loop protocols. Closed-loop treatments evaluated transient temperature increases of 5, 10, 15, or 22°C above baseline. The temperature controlled ablation diameters were conditionally different than the open-loop treatments and closed-loop treatments generally produced smaller ablations. Closed-loop control enabled the investigation of treatments with steady state 42°C hyperthermic conditions which were not feasible without active feedback. Baseline closed-loop treatments at 20°C resulted in ablations measuring 9.9 ± 0.3mm in diameter while 37°C treatments were 20% larger (p < 0.0001) measuring 11.8 ± 0.3mm indicating that this protocol induces a thermally mediated biological response. A closed-loop control algorithm which modulated the delay between successive pulse waveforms to achieve stable target temperatures was demonstrated. Algorithmic control enabled the evaluation of specific treatment parameters at physiological temperatures not possible with open-loop systems due to excessive Joule heating. Irreversible electroporation is generally considered to be a non-thermal ablation modality and temperature monitoring is not part of the standard clinical practice. The results of this study indicate ablative outcomes due to exposure to pulses on the order of one microsecond may be thermally mediated and dependent on local tissue temperatures. The results of this study set the foundation for experiments in vivo utilizing temperature control algorithms.