Transfer Of Immunoglobulins Research Articles

Acceptance and uptake of vaccines against tetanus, influenza, pertussis, and COVID-19 among pregnant and postpartum women in low- and middle-income countries: a systematic review and meta-analysis protocol

BackgroundPregnant women, fetuses, and neonates are particularly vulnerable to vaccine-preventable diseases (VPDs). These VPDs are associated with high morbidity and mortality among expectant mothers and their fetuses and neonates. Vaccination during pregnancy can protect the expectant mother from VPDs to which she may be especially vulnerable while pregnant. In addition, the passive transfer of maternal neutralizing immunoglobulin G (IgG) and secretory immunoglobulin A (IgA) also protects the fetus against congenital infections and may further protect the neonate from infection during the first few months of life. Despite this, coverage of recommended maternal vaccines remains suboptimal globally, especially in resource-constrained settings. Determinants of vaccine acceptance and uptake are frequently understudied in low- and middle-income countries (LMICs) and among specific groups such as pregnant and postpartum women. This proposed systematic review will assess the acceptance and uptake of vaccines against tetanus, influenza, pertussis, and COVID-19 among pregnant and postpartum women in LMICs.MethodsA Boolean search strategy employing common and medical subject heading (MeSH) terms for tetanus, influenza, pertussis, and COVID-19 vaccines, as well as vaccine acceptance, hesitancy, together with uptake, pregnancy, and postpartum, will be used to search electronic databases for relevant literature published between 2009 and 2024. Only studies conducted in LMICs that investigated determinants of acceptance, hesitancy, and uptake of tetanus, influenza, pertussis, and COVID-19 vaccines among pregnant and postpartum women will be eligible for inclusion in the review. The quality and the risk of bias of all eligible full-text articles will be assessed using the Joanna Briggs Institute’s (JBI) critical appraisal tools.DiscussionThis protocol proposes a systematic review and meta-analysis that aims to assess the uptake of maternal vaccines and to systematically appraise and quantify determinants of the acceptance and uptake of recommended vaccines during pregnancy and postpartum in LMICs. A better understanding of these factors and how they influence maternal vaccine decision-making will enable public health practitioners as well as global and national policymakers to design more effective interventions as we look towards expanding the scope and reach of maternal immunization programs.

Antibody-mediated autoimmunity in symptom-based disorders: position statement and proceedings from an international workshop.

A 2-day closed workshop was held in Liverpool, United Kingdom, to discuss the results of research concerning symptom-based disorders (SBDs) caused by autoantibodies, share technical knowledge, and consider future plans. Twenty-two speakers and 14 additional participants attended. This workshop set out to consolidate knowledge about the contribution of autoantibodies to SBDs. Persuasive evidence for a causative role of autoantibodies in disease often derives from experimental "passive transfer" approaches, as first established in neurological research. Here, serum immunoglobulin (IgM or IgG) is purified from donated blood and transferred to rodents, either systemically or intrathecally. Rodents are then assessed for the expression of phenotypes resembling the human condition; successful phenotype transfer is considered supportive of or proof for autoimmune pathology. Workshop participants discussed passive transfer models and wider evidence for autoantibody contribution to a range of SBDs. Clinical trials testing autoantibody reduction were presented. Cornerstones of both experimental approaches and clinical trial parameters in this field were distilled and presented in this article. Mounting evidence suggests that immunoglobulin transfer from patient donors often induces the respective SBD phenotype in rodents. Understanding antibody binding epitopes and downstream mechanisms will require substantial research efforts, but treatments to reduce antibody titres can already now be evaluated.

Salivary IgG and IgA in newborn calves and the possible role in the assessment of passive immunity transfer.

The transfer of immunoglobulins from the mother to newborns is widely recognized as a critical event for safeguarding offspring against potentially life-threatening infectious diseases. Mainly for this reason, this study aimed to assess the concentrations of immunoglobulin G (IgG) and immunoglobulin A (IgA) in the saliva of newborn calves and explore its potential use for monitoring passive immunity transfer from cows to calves, as also to evaluate how colostrum intake affects serum and saliva IgG and IgA concentrations. The quality of colostrum samples was evaluated using an optical refractometer before administration to the calves. Saliva and blood samples from 24 calves were obtained at the day of birth (T0) and 2 days after (T2) for determination of serum concentrations of total protein by refractometer, IgG and IgA (both on serum and saliva) by ELISA test. Positive correlations were observed between salivary IgA at T2 and salivary IgG at T2. A significant increase in both IgG and IgA levels in calf serum and saliva was noted. Salivary IgA levels can reflect salivary IgG levels. These findings suggest the potential utility of IgA in monitoring passive immunity transfer, and do not exclude saliva as an alternative, practical, and non-invasive matrix for assessing passive immunity transfer.

FcRY is a key molecule controlling maternal blood IgY transfer to yolks during egg development in avian species.

Maternal immunoglobulin transfer plays a key role in conferring passive immunity to neonates. Maternal blood immunoglobulin Y (IgY) in avian species is transported to newly-hatched chicks in two steps: 1) IgY is transported from the maternal circulation to the yolk of maturing oocytes, 2) the IgY deposited in yolk is transported to the circulation of the embryo via the yolk sac membrane. An IgY-Fc receptor, FcRY, is involved in the second step, but the mechanism of the first step is still unclear. We determined whether FcRY was also the basis for maternal blood IgY transfer to the yolk in the first step during egg development. Immunohistochemistry revealed that FcRY was expressed in the capillary endothelial cells in the internal theca layer of the ovarian follicle. Substitution of the amino acid residue in Fc region of IgY substantially changed the transport efficiency of IgY into egg yolks when intravenously-injected into laying quail; the G365A mutant had a high transport efficiency, but the Y363A mutant lacked transport ability. Binding analyses of IgY mutants to FcRY indicated that the mutant with a high transport efficiency (G365A) had a strong binding activity to FcRY; the mutants with a low transport efficiency (G365D, N408A) had a weak binding activity to FcRY. One exception, the Y363A mutant had a remarkably strong binding affinity to FcRY, with a small dissociation rate. The injection of neutralizing FcRY antibodies in laying quail markedly reduced IgY uptake into egg yolks. The neutralization also showed that FcRY was engaged in prolongation of half-life of IgY in the blood; FcRY is therefore a multifunctional receptor that controls avian immunity. The pattern of the transport of the IgY mutants from the maternal blood to the egg yolk was found to be identical to that from the fertilized egg yolk to the newly-hatched chick blood circulation, via the yolk sac membrane. FcRY is therefore a critical IgY receptor that regulates the IgY uptake from the maternal blood circulation into the yolk of avian species, further indicating that the two steps of maternal-newly-hatched IgY transfer are controlled by a single receptor.

Assessing colostral and serum immunoglobulin G in alpacas using Brix refractometry and total serum protein

The adequate transfer of passive immunity is a critical factor in neonatal development and survivability. Although well documented in the dairy and equine industries, the recognition of inadequate immunoglobulin transfer on-farm and its impact on the ability of alpaca cria to thrive is largely unknown. Colostrum samples were collected from female alpaca within 24 h of parturition by the owners and whole blood collected from cria by the investigators between 1 and 7 days of age. Direct IgG concentration of milk and serum was determined using radial immunodiffusion assay (RID) and was indirectly estimated using optical and digital Brix refractometry for total solids and clinical refractometry for total serum protein. There was a strong correlation between optical and digital Brix refractometry, and colostral IgG concentration determined by RID. There was a moderate correlation between serum IgG concentration determined by RID and total serum protein in crias. Optical and digital Brix refractometry for colostral IgG estimation and total serum protein for serum IgG estimation are reliable, accurate and easy-to-use tools that can be used on-farm by trained, competent technicians to assess a failure of passive transfer in alpacas. A pilot study at one property only was performed, due to COVID-19 travel restriction interference. Further research is required to determine the reference intervals for these tools to be practical.

Murine models of idiopathic inflammatory myopathies.

Idiopathic inflammatory myopathies (IIMs) are divided into polymyositis and dermatomyositis (DM) with specific cutaneous manifestation. Several myositis-specific autoantibodies (MSAs) have been identified in IIMs and were found to be associated with distinct clinical features, including anti-synthetase syndrome (ASyS) and immune-mediated necrotizing myopathy (IMNM). Moreover, MSA-related clinical features have been identified even within DM. Although MSAs are valuable for the diagnosis of IIMs, the pathogenic roles of these antibodies remain unknown. To investigate the pathogenesis of IIMs, classical murine models of autoimmune myositis, experimental autoimmune myositis, and C protein-induced myositis have been established by immunization with muscle-specific antigens, myosin, and myosin-binding skeletal C protein, respectively. To according to MSA-related autoimmunity, a murine model of ASyS was generated by immunization with a murine recombinant histidyl-transfer RNA (tRNA) synthetase, Jo-1, in which muscle and lung inflammation are induced depending on acquired immunity. Furthermore, it was found that the transfer of human Immunoglobulin G (IgGs) from patients with IMNM, comprising anti-signal recognition particles and anti-3-hydroxy-3-methylglutaryl coenzyme A reductase antibodies, induced complement-mediated myositis in recipient mice. We found that CD8+ T cell-mediated myositis can be established depending on autoimmunity against transcriptional intermediary factor 1γ (TIF1γ), an autoantigen for MSAs induced by recombinant human TIF1γ immunization. These new murine models reflecting MSA-associated IIMs will reveal the immunological mechanisms underlying IIMs.

Climatic and calf-related risk factors associated with failure of transfer of passive immunity in Holstein calves in a hot environment.

This retrospective observational study aimed to assess the effect of temperature-humidity index (THI) at calving and in the last trimester of pregnancy and calve-related factors affecting passive transfer of maternal immunoglobulin using Brix refractometry in Holstein calves. Blood samples from 4411 Holstein calves from a single large dairy farm in spring 2022 were used. A subset of data containing 6318 calvings was used to determine the effect of climatic conditions on the occurrence of agammaglobulinemia. Risk factors predictive of failure of passive transfer (FPT) were calculated using multiple logistic models. Females were 1.4 times more likely not to have FPT (56%; Brix% > 8 equivalent to ≥ 10g/L IgG) than males (47.2%). Calves born as singles increased the likelihood of not presenting FPT (52.6%) than calves born as twins (42.9%). Calves from cows with no dystocic delivery had a lower risk for FPT (odds ratio = 2.3) than calves from cows with dystocia. Agammaglobulinemia was 1.5 and 1.8 times more likely to occur in calves with THI ≥ 80 and ≥ 82 in the last trimester of gestation and at calving, respectively, than in calves not experiencing heat stress. Agammaglobulinemia was twice as likely to occur in male than in female calves. Calves with birth weight ≥ 37kg and gestation length ≥ 275kg were less likely to present agammaglobulinemia than lighter calves and calves with shorter gestation periods. This study raises questions for management practices in Holstein calves undergoing in utero heat stress and around calving to avoid agammaglobulinemia.

Dose-response of inactivated yeast in diets of late gestating and lactating gilts on immunoglobulin transfer and offspring preweaning growth performance.

Fifty gilts (initial body weight [BW] 190.7 ± 4.2kg) were recruited on day 85 of gestation and were used until day 19 of lactation to assess the dose-response of inactivated yeast via hydrolyzation (HY) inclusion on offspring growth and immunoglobulin (Ig) transfer prior to weaning. Gilts were assigned to one of the 5 experimental diets: a control with no HY (HY0) or inclusion of 0.25% (HY0.25), 0.5% (HY0.5), 1.0% (HY1.0), or 1.2% (HY1.2) HY. Gilts were weighed on days 85 and 110 of gestation and days 1 and 19 (weaning) after farrowing. Offspring were weighed on days 1 and 19 of age. On lactation day 1 (approximately 24h after farrowing), colostrum, gilt plasma, and plasma from 2 median BW piglets were collected and on day 19, plasma from each gilt and 2 median BW piglets per litter were collected for determination of Ig concentrations. Contrast statements were used to assess the linear, quadratic, cubic, and quartic effects of HY inclusion. The inclusion of HY had minimal effects on gilt BW or litter characteristics at birth (total number born and born alive, piglet birth weight). Lactation average daily feed intake of the gilts tended to increase then decrease with increasing HY inclusion (quadratic; P = 0.085). Piglet preweaning average daily gain (linear, quadratic, and quartic; P < 0.05) and BW at weaning (quadratic and quartic; P < 0.05) increased then decreased with increasing HY inclusion. On lactation day 1, colostrum and gilt plasma Ig concentrations were not affected by dietary treatment (P > 0.10) but piglet IgA and IgM decreased then increased with HY inclusion level (cubic; P < 0.05). On lactation day 19, piglet plasma IgG tended to increase with HY inclusion (linear; P = 0.099). In summary, increasing HY inclusion in late gestating and lactating gilt diets improved immune transfer in the first 24h after birth and piglet preweaning growth rates and BW at weaning. Therefore, maternal feeding of HY could be used as a strategy to improve offspring immunocompetence and BW at weaning, with possible carryover benefits for the postweaning phase.

Difructose Anhydride and Passive Immunity Effects on Passive Immune Transfer and Performance of Feeding Difructose Anhydride to Neonatal Calves.

The objective of this study was to assess the potential effects of supplementing difructose anyhdride III (DFAIII) during the first days of life on the absorption of immunoglobulin G (IgG) and growth performance of calves early in life fed colostrum with a high IgG concentration. Sixty-six healthy new-born Holstein calves were randomly assigned to three treatments consisting of no supplementation (control), supplementation of 12 g/d (DFA12), or 36 g/d (DFA36) of DFAIII during the first 7 d of life via colostrum and milk replacer (MR). Calves were separated from dams at birth and bottle-fed colostrum in two meals, each targeting 2.5 L within the first 18 h of birth. Colostrum had been previously collected from other dams (and preserved frozen) within the first 2 h of calving and had a Brix value ≥32%. Daily consumption of starter concentrate and MR (and colostrum on the first day) were individually monitored. Calves were body weighed using an electronic scale at birth and on a weekly basis thereafter until the end of study at 42 d of age. A sample of colostrum fed to each calf and a blood sample from the jugular vein of the calves were collected at 12 and 24 h of life to determine the IgG concentration. The mean colostrum IgG concentration fed in the current study was 110 ± 33.7 g/L (mean ± SD). No differences in animal performance were found among the treatments. Calves on all treatments consumed the same amount of colostrum with a similar concentration of IgG, and thus the amount of IgG consumed was also similar. Serum IgG concentrations were greater at 24 than at 12 h but did not differ among treatments. However, the apparent efficiency of absorption of colostral immunoglobulins was greater in DFA12 and DFA36 at 12 h of life than in control calves, with no differences observed at 24 h. Even when feeding high-quality colostrum, in terms of IgG concentration, supplementation with difructose anhydride III may pose an additional advantage in promoting the passive transfer of immunoglobulins in neonatal Holstein calves during the first 12 h of life.

Total and pathogen-specific serum Immunoglobulin G concentrations in neonatal beef calves, Part 1: Risk factors.

Maternal antibodies, delivered to the calf via colostrum, are crucial to prevent calfhood diseases and death. However, knowledge regarding the factors influencing this transfer of total and specific Immunoglobulin G (IgG) against common enteric and respiratory disease pathogens under current production conditions is sparse. The objectives of this study were to determine risk factors influencing total and pathogen-specific immunoglobulin G (IgG) concentrations against Escherichia coli (E. coli), bovine Rotavirus (BRoV), Cryptosporidium parvum (C. parvum), Bovine Viral Diarrhea Virus type 1 and 2 (BVDV), Parainfluenza Virus Type 3 (PI-3), Bovine Respiratory Syncytial Virus (BRSV), and Bovine Herpesvirus type 1 (BHV-1) in the serum of newborn beef calves. A total of 420 serum samples were collected from 1- to 7-day-old beef calves born on 6 farms in Alberta, Canada. Samples were analyzed by radial immunodiffusion for total IgG concentration and by enzyme-linked immunosorbent assays for pathogen-specific IgG concentrations against E. coli, BRoV, C. parvum, BVDV, PI-3, BRSV, and BHV-1. Multivariable multilevel linear and logistic regression models were built to evaluate dam- and calf-level risk factors associated with total and pathogen-specific IgG concentrations, failed transfer of passive immunity (FTPI; serum IgG < 10g/L), and inadequate transfer of passive immunity (ITPI; serum IgG < 24g/L). Farm was included as a random effect in all models to account for clustering at the herd level. Of the 420 calves included in this study, 5% (n=20) and 18% (n=75) of calves had FTPI and ITPI, respectively. Receiving colostrum intervention (i.e., being fed colostrum or colostrum product by either bottle or tube) was the most consistent risk factor for low total IgG concentration and significantly increased the odds of FTPI (Odds ratio (OR): 6.1, 95% CI: 2.0-18.9) and ITPI (OR: 4.8, 95% CI: 2.1-10.8). Calves born to cows consistently had higher pathogen-specific IgG concentrations (P<0.0001), compared to calves born from heifers, and calves born to vaccinated dams had significantly higher BRoV, BVDV, and BHV-1-specific IgG concentrations. Interestingly, E.coli-specific IgG concentrations were associated with dam vaccination only in cows but not in heifers, which was likely due to differing vaccination strategies used. This study highlights the need to review and refine protocols with respect to dam vaccination and colostrum intervention on cow-calf operations.

106 Using Postbiotic Saccharomyces Cerevisiae Yeast in Gestation and Lactation Diets of Gilts to Improve Immunoglobulin Transfer and Offspring Growth Pre-Weaning

Abstract Gilts [n = 50; initial body weight (BW) 190.7 ± 4.2 kg] were used to determine the optional inclusion level of postbiotic Saccharomyces cerevisiae in late gestation and lactation diets to improve immunoglobulin transfer and piglet growth performance during the suckling period. Gilts were assigned to one of five (n = 10) diets: standard gestation and lactation diets (Y0) or diets with postbiotic yeast included at either 0.25% (Y0.25), 0.5% (Y0.5), 1.0% (Y1), or 1.2% (Y1.2). Diets were fed from d 85 of gestation until weaning (d 21 after farrowing). During gestation gilts were fed 2.6kg/d and feed was offered ad libitum during lactation. Gilt BW was recorded on d 85 and 110 of gestation, and gilt and piglet BW were recorded on d 1 and 21 of lactation. Twenty-four hours after farrowing, plasma was collected from each gilt and two median BW piglets per litter via orbital sinus. Additionally, 50 mL of colostrum was collected in equal parts from all functional teats on the right side. On d 21, plasma samples were collected from each gilt and two median BW piglets per litter. Concentrations of immunoglobulin (Ig) A, G, and M were quantified in plasma and colostrum. Data were analyzed in SAS with treatment as the main effect. Linear, quadratic, and yeast vs. no yeast contrast statements were used to assess responses to dietary inclusion of yeast. Time (minutes) between farrowing (defined as birth of the first piglet, confirmed by video recording) and sampling was used as a covariate for d 1 Ig concentrations. There were no differences in number of piglets born alive (12.5 ± 0.9), birth weight (1.40 ± 0.45kg), or gilt BW change during lactation (-7.98 ± 6.68kg). Lactation average daily feed intake tended to be 14.6% greater for gilts provided yeast versus no yeast (5.11 vs. 4.46 ± 0.31kg, respectively; P = 0.069). At weaning, offspring from Y0.25 fed gilts had greater BW than piglets from Y0- or Y1.2-fed gilts (6.48 vs. 5.66 and 5.82 kg ± 0.16, respectively; P &amp;lt; 0.001), with all other treatments intermediate. On d 1, colostrum and gilt plasma Ig concentrations were unaffected by diets. However, yeast increased piglet plasma concentrations of IgM (linear; P = 0.034) and tended to increase IgA (linear; P=0.093), and total Ig (linear; P = 0.074). On d 21, gilts fed yeast-containing diets tended to have greater plasma IgA (yeast vs. no yeast and quadratic; P = 0.063 and P = 0.076, respectively), but IgM was greater for gilts fed Y0 (yeast vs. no yeast; P &amp;lt; 0.05). On d 21, piglet plasma IgA tended to be greater when gilts were provided yeast-containing diets (yeast vs. no yeast; P = 0.069). Therefore, postbiotic Saccharomyces cerevisiae was effective in improving maternal transfer, with piglet IgA and IgM concentrations increasing linearly with increasing yeast inclusion, while Y0.25 also improved piglet growth performance during the suckling phase.

Cellular and humoral immunity of broilers subjected to posthatch fasting and a prestarter diet containing conjugated linoleic acid

This study aimed to evaluate the immunity of chickens up to 35 d subjected to posthatch fasting and supplementation with conjugated linoleic acid (CLA). A total of 320 chicks were housed in a completely randomized design with a 2×2 factorial arrangement (0 or 12 h of fasting×0.000 or 0.025% CLA in a prestarter diet), totaling 4 treatments (No-F-12 h; F-12 h; No-CLA; CLA) with 8 replicates of 10 birds each. The relative weights (% body weight) of the spleen and bursa were determined 12 h posthatch (Post-12 h) and then weekly. Immunoglobulin Y (IgY) titers against Newcastle disease virus (NDV) were measured by ELISA in the yolk sac contents Post-12 h and in the serum weekly. Hypersensitivity to phytohemagglutinin (PHA) inoculation was evaluated by toe-web swelling response on d 13 and 34, 4 times a day (after 3 h, 6 h, 12 h, and 24 h inoculation, respectively, PHA-3 h, PHA-6 h, PHA-12 h, and PHA-24 h). The data were subjected to analysis of variance (P < 0.05). F-12h reduced the Post-12 h relative weight of the spleen, and CLA reduced the relative weight of the bursa at this stage and at 28 d. At 13 d, F-12 h reduced PHA-3 h, whereas PHA-12 h was increased by CLA. At 34 d, CLA reduced PHA-3 h. A greater reaction was observed in the No-F-12 h-CLA chicks, for the PHA-24 h. In the Post-12 h evaluation, F-12h reduced, whereas CLA increased NDV-specific IgY titers in the yolk sac. No-F-12 h-No-CLA chicks had the lowest serum titers. At 21 d, F-12 h-CLA chicks exhibited the highest serum titers. Titers were higher in the F-12 h-No-CLA chicks, when compared to other treatments. At 28 d, fasting reduced the titers. In conclusion, F-12 h and CLA accelerated the transfer of immunoglobulins from the yolk sac to the serum. F-12 h impairs cellular immunity, whereas CLA favors it.

Neutrophils infiltrate sensory ganglia and mediate chronic widespread pain in fibromyalgia

Fibromyalgia is a debilitating widespread chronic pain syndrome that occurs in 2 to 4% of the population. The prevailing view that fibromyalgia results from central nervous system dysfunction has recently been challenged with data showing changes in peripheral nervous system activity. Using a mouse model of chronic widespread pain through hyperalgesic priming of muscle, we show that neutrophils invade sensory ganglia and confer mechanical hypersensitivity on recipient mice, while adoptive transfer of immunoglobulin, serum, lymphocytes, or monocytes has no effect on pain behavior. Neutrophil depletion abolishes the establishment of chronic widespread pain in mice. Neutrophils from patients with fibromyalgia also confer pain on mice. A link between neutrophil-derived mediators and peripheral nerve sensitization is already established. Our observations suggest approaches for targeting fibromyalgia pain via mechanisms that cause altered neutrophil activity and interactions with sensory neurons.

In utero priming of fetal immune activation: Myths and mechanisms

Mechanisms of fetal immune system development in utero remain incompletely elucidated. Protective immunity, the arm of reproductive immunology concerned with the progressive education of the fetal immune system as pregnancy advances, allows for programming of the immune system and immune maturation in utero and provides a responsive system to respond to rapid microbial and other antigenic exposure ex utero. Challenges in studying fetal tissues, immune system development, and the contributions of various endogenous and exogenous factors to this process are difficult to study as a progressive sampling of fetal biological samples is impractical during pregnancy, and animal models are limited. This review provides a summary of mechanisms of protective immunity and how it has been shaped, from transplacental transfer of immunoglobulins, cytokines, metabolites, as well as antigenic microchimeric cells to perhaps more controversial notions of materno-fetal transfer of bacteria that subsequently organize into microbiomes within the fetal tissues. This review will also provide a quick overview of future direction in the area of research on fetal immune system development and discusses methods to visualize fetal immune populations and determine fetal immune functions, as well as a quick look into appropriate models for studying fetal immunity.

The role of anti-E. coli antibody from maternal colostrum on the colonization of newborn dairy calves gut with Escherichia coli and the development of clinical diarrhea

Escherichia coli (E. coli) is an early organism in the colonization of mucosal tissue and has an influence on the development of mucosal and systemic inflammatory responses. To understand the impact of transfer of Immunoglobulin G (IgG), E. coli-specific antibody and general immune factors to the calf on the gut microbiota, an observational study that followed the temporal colonization of the intestine with E. coli as correlated with the total quantity of IgG and the antibody recognizing E. coli, was conducted in newborn Holstein calves. The calves were subjected to the “gold standard” of colostrum management, including dam vaccination prior to delivery to prime colostrum. Twenty Holstein dams, producing an adequate quantity of good-quality colostrum following uncomplicated deliveries of healthy female offspring, were utilized in this study. Each calf was monitored and scored daily for diarrhea. Blood and fecal samples were collected from calves on day (D) D1 (before colostrum intake), then D3, D7, D14 and D28 after colostrum feeding. E. coli isolated from fecal samples of the calves were assessed for virulence genes and the quantity per gram of feces using conventional Polymerase chain reaction and Real-time polymerase chain reaction quantitative, respectively. The total quantity of plasma IgG and the titer of E. coli-binding antibody were measured by enzyme-linked immunosorbent assay. The peak in diarrhea in the calves was observed on D12. No IgG or E. coli-binding antibody were detected prior to colostrum intake (D1). Both total IgG and binding antibody sharply increased by D3. Total and binding IgG declined with calf age. The total number of bacteria (16S rRNA) in feces was similar between D1 and D14. The number of total bacteria increased over the period from D14 to D28. The number of E. coli was minimal on D1. The number of E. coli increased during the period D3-D7. A decrease in the number of E. coli per gram of feces declines between D14and D28. The stable toxin gene was frequently identified in the E. coli isolated from the calves. Further, E. coli K99 was not identified in this study. While the gut was colonized by E. coli early in life corresponding to the peak level of IgG and specific antibody, a decreasing number of E. coli were observed as the passive immunity waned. This suggests that the function of the passive antibody may be to control colonization and seed innate immune function that then stabilizes the level of E. coli in the digestive tract of calves.

Effects of broiler breeder age on immune system development of progeny

The objective of this study was to determine the effects that breeder age has on digestive and immune system development; the transfer of immunoglobulins to egg yolk, yolk sac, and neonate chicks; and the immune response of chicks up to 35 days old. Three ages (32, 42, and 52 weeks) of Hubbard breeders were studied with ages as treatments. A total of 425 eggs were weighed for each of the three treatments and incubated. After hatching, a total of [...]

Effects of freezing storage on the stability of maternal cellular and humoral immune components in porcine colostrum

The placental structure of sows is epitheliochorial and prevents maternal serum immunoglobulin transfer to the fetus; therefore, the piglet relies on the ingestion of colostrum to acquire passive immunity. Colostral antibody-mediated and cell-mediated immunity contribute to immunity in piglets. However, little is known about the effects of freezing at −20 °C on colostral immune components during short-term storage, whether this will somehow compromise the acquisition of passive immunity of newborn piglets fed with this colostrum and the humoral immunity in porcine colostrum, and to possible shifts in immunological levels in colostrum collections during the colostral period. Based on the average concentration of immunoglobulin, frozen and fresh colostrum did not differ significantly. Overall, there were no storage differences in total macrophages, granulocytes, and NK cells. However, the frozen colostrum presented T lymphocyte subsets and B lymphocytes significantly lower than the fresh colostrum (p ≤ 0.05). Therefore, to sustain higher piglet survival rates, B cells may be a selective strategy to ensure immune defense to neonatal piglets. According to our findings, colostrum can be stored by freezing at −20 °C for up to 30 days and surplus porcine colostrum can be collected from the sow up to eight hours after the start of farrowing.

Transplacental transfer of total immunoglobulin G and antibodies to Plasmodium falciparum antigens between the 24th week of gestation and term

Full-term newborns have antibody (Ab) repertoires and levels similar to their mothers to help protect them from environmental pathogens. Unfortunately, preterm babies, especially those born < 34 weeks, have reduced levels of protective antibodies. In Africa, antibodies to Plasmodium falciparum are important in protection from malaria. This study investigated the transfer of total IgG and antibodies to 9 P. falciparum antigens and tetanus toxoid between 24 weeks and term. Paired maternal and cord samples from 166 preterm (24–37 weeks) and 154 term deliveries were used. Transfer efficiency was expressed as the ratio of Ab levels in cord to maternal plasma (CMR). At 24–25 weeks, CMR ranged from 0.31 to 0.94 for the different antigens; the rate of transfer was similar for all antigens between 24 and 40 weeks; resulting in median CMR of 0.49–0.95 at term. Babies of mothers with hypergammaglobulinemia and normal IgG levels had similar amounts of IgG, supporting data that saturation of the neonatal Fc-receptor occurs at ~ 16 mg IgG/ml. Thus, babies born prior to 34–35 weeks in Africa are likely to have reduced Ab levels to some, but not all antigens. Since IgG transfer is Fc-mediated, why differences exist in CMR among the antigens warrants further investigation.

Epidemiology and characteristics of SARS-CoV-2 infection in the newborn and pregnant woman. Transplacemental transfer of immunoglobulins

IntroductionSARS-CoV-2 infection during pregnancy and its impact on the newborn were, in the first months of the pandemic, unknown. Recent studies have provided information on the clinical involvement in the newborn and its evolution.This work shows how passive immunity varies in the newborn in relation to the moment of maternal SARS-CoV-2 infection during pregnancy. Population and methodObservational, prospective and longitudinal study in a third level hospital. Epidemiological and clinical data from mothers and their newborns were collected from May 2020 to June 2021. ResultsA total of 109 mothers and 109 neonates have been included. 28.4% of maternal infections were in the first trimester, 24.8% during the second and 58.8% in the third. 56% of maternal infections were symptomatic and only one pregnant woman with severe respiratory infection was admitted to intensive care. The mean gestational age of the newborns was 39 weeks, with a mean weight of 3232 g and a head circumference of 35 cm. Eight newborns born from mothers with SARS-CoV-2 required admission to the neonatal ICU: 2 due to jaundice, 2 due to respiratory distress, 1 due to moderate prematurity, and 3 due to other causes unrelated to infection attributable to SARS-CoV-2. IgG-type antibodies were positive in 56.9% of newborns. Of the mothers infected during the 1 st trimester, IgG were positive in 32.2% of the newborns, in the second trimester 81.5% were positive and in the third 58.8%. No neonate had positive IgM. ConclusionsSARS-CoV-2 infection during pregnancy provides IgG antibodies to half of newborns. The presence of antibodies in the newborn is more likely when the infection has occurred in the second trimester of pregnancy.

Assaying the concentration of immunoglobulin G in colostrum from females postpartum and serum from neonatal calves of buffaloes (Bubalus bubalis)

Neonatal ruminants are born without any humoral immunity due to lack of placental transfer of immunoglobulins during gestation. This predisposes the newborn buffalo calves to a high incidence of morbidity and mortality on exposure to infectious agents. Colostrum is the first milk produced by the females after calving and is a rich source of immunoglobulins, especially immunoglobulin G (IgG). The immunocompetence of the neonates can be boosted by feeding them sufficient amount of good quality colostrum within a few hours after birth. Optimal colostrum management at a farm not only reduces the occurrence of diseases among the younger stock but also enhances their growth performance and productivity once they are adults. In the present study, eighty animals at a Murrah buffalo farm were screened for the concentration of IgG in the colostrum collected from recently parturated females and in the serum collected from their calves within 6 to 12 h of colostrum consumption to determine the status of transfer of passive immunity. Indirect ELISA was used to estimate the IgG levels. The overall mean (range) of colostral and serum IgG concentration was found to be 50.44±3.36 (12.71 to 227.78) and 10.85±0.62 (0.25 to 19.88) mg/ml, respectively for all the 80 animals. Routine screening of buffaloes, in a similar way, will help to reduce calf deaths due to immunodeficiency.