Effect of variety differences on the meat quality traits and flavour of donkey meat based on transcriptomic and metabolomic analysis.

Deziyangxin (DZYX) is a classical Tibetan multi-herb prescription recorded in Jing Zhu Ben Cao and traditionally used for conditions characterized by blood stasis, phlegm-heat accumulation, and tumor-like masses. Despite its long-standing clinical use, the pharmacological basis underlying its potential anti-tumor activity remains insufficiently understood. This study aimed to evaluate the anti-tumor efficacy of DZYX in non-small cell lung cancer (NSCLC) and to explore its potential mechanisms with emphasis on tumor immune microenvironment modulation. Serum pharmacochemistry was performed using LC-MS/MS to identify circulating constituents of DZYX. A syngeneic Lewis lung carcinoma (LLC) mouse model was established in mice, which were treated with DZYX by oral gavage at 78.5mg/kg or 157mg/kg for 25 days. Tumor growth and histopathology were evaluated, while transcriptomic analysis, network pharmacology, flow cytometry, and immunohistochemistry were used to investigate molecular pathways and immune cell infiltration. LC-MS/MS identified 167 circulating components derived from DZYX. DZYX treatment significantly suppressed tumor growth and induced tumor necrosis in LLC-bearing mice. Transcriptomic and enrichment analyses indicated modulation of chemokine signaling, T-cell activation, and innate immune pathways. Flow cytometry and immunohistochemistry further demonstrated increased infiltration of CD4+ and CD8+ T cells, NK cells, and B cells within tumor tissues and spleens. Enhanced intratumoral Granzyme B and IFN-γ signals suggested increased cytotoxic immune activity. DZYX exerts anti-tumor activity in NSCLC associated with remodeling of the tumor immune microenvironment and multi-pathway molecular regulation. These findings provide mechanistic support for the traditional use of DZYX in tumor-related disorders and highlight its potential as an immunomodulatory candidate for lung cancer therapy.

Perkinsus spp. are pathogenic protistan parasites that pose a significant threat to the aquaculture of the Hong Kong oyster (Crassostrea hongkongensis). This study investigated the transcriptional response of the Hong Kong oyster digestive gland to natural Perkinsus spp. infection. The parasite species was identified via PCR, while infection prevalence, tissue-specific abundance, and infection grade were assessed using Ray's Fluid Thioglycollate Medium (RFTM) culture. Transcriptomic analysis was performed to compare gene expression profiles among uninfected (Neg), lightly infected (L), and light-to-moderately infected (LM) oyster digestive glands. Results confirmed the presence of P. beihaiensis, with an overall infection prevalence of 85.5%. The digestive gland exhibited the highest infection prevalence (80.1%) and abundance (1880.0 ± 144.2 cells/g) compared to other tissues (gills, mantle, and siphon). Transcriptomic analysis of 12 samples (4 biological replicates per Neg, L, and LM group) using an Illumina Novaseq 6000 platform generated 102,827 unigenes. A total of 531 differentially expressed genes (DEGs) were common to both infected groups relative to the Neg group. GO and KEGG enrichment analyses highlighted key biological processes and pathways: up-regulated DEGs were associated with the cell cycle, antigen processing and presentation, and signal transduction, while down-regulated DEGs were linked to cholesterol metabolism, phagosome function, and fat digestion and absorption. qRT-PCR validation of 8 DEGs confirmed consistent expression patterns with RNA-seq data. These findings elucidate the tissue-specific infection patterns of P. beihaiensis in Hong Kong oysters in the digestive gland and provide critical insights into host-parasite interactions.

Transcriptomic analyses of piperitone inhibited sprouting of 'Yan 25' sweet potato storage roots.

Mechanism of N-butanol extract of Pulsatilla decoction protected vaginal epithelial cells against Candida albicans infection by HIF-1α signaling and glucose metabolism revealed by transcriptomics and molecular docking.

Wild tomato species Solanum habrochaites (SH) exhibits superior drought tolerance compared with cultivated Ailsa craig (AC). This study investigated the molecular mechanisms underlying this trait through integrated transcriptomic and metabolomic analysis, focusing on the regulatory role of SlTAT in coordinating antioxidant and metabolic responses. We employed comparative physiological assays, virus-induced gene silencing (VIGS) of SlTAT, and integrated multi-omics analysis (RNA-seq and LC-MS/MS) to characterize drought response mechanisms in SH versus AC under controlled drought conditions. SlTAT was identified as a critical regulator enriched in amino acid and alkaloid biosynthesis pathways. Silencing SlTAT in AC significantly impaired drought tolerance (P < 0.01), concurrently altering expression of antioxidant and secondary metabolism genes and reducing accumulation of protective metabolites. SlTAT enhances drought tolerance by synchronizing antioxidant defences with metabolic reprogramming in amino acid and alkaloid pathways. These findings provide novel targets for improving crop resilience through secondary metabolite engineering.

Integrated transcriptome and metabolome analysis reveals new insights into acclimation of sea cucumber Stichopus monotuberculatus to hypo-osmotic stress.

Unraveling novel transposable elements (TEs)-driven gene dysregulation in non-small cell lung cancer (NSCLC) by integrated transcriptomic and TEs analysis.

Transcriptome and alternative splicing analyses uncover immune-centric pathogenesis in periodontitis versus barrier-dysfunction-driven pathogenesis in peri-implantitis.

Unveiling the mechanisms of kojic acid-induced resistance in postharvest tomato against Pectobacterium carotovorum subsp. carotovorum through biochemical and transcriptomic analysis

Identification of key genes associated with butyrate metabolism in keratoconus through Mendelian randomization and transcriptomic analysis.

Whole-cell biocatalysis of HMF into BHMF by Aspergillus niger: Process development and identification of key genes.

Physiological effects of long-term saline-alkaline stress on the gills of Acanthopagrus latus: A combined analysis of transcriptomics and metabolomics.

Phytotoxicity beyond bioaccumulation: Hexafluoropropylene oxide homologues disrupt Arabidopsis thaliana growth and reproduction via metabolic and hormonal dysregulation.

Transcriptional coordination enhances folate biosynthesis in dry common bean grains during storage.

Di-tert-Butylphenol (2,4-DTBP) and its analogs disrupt thyroid hormone signaling via activation of retinoid X receptor α.

Activating NK cell immunity against prostate cancer with Hedysarum polybotrys Hand.-Mazz.: A multi-omics integration of computational prediction and experimental validation on PI3K/AKT and PD-1/PD-L1 axes.

Metabolic response of Haloferax mediterranei to different amino acids as a nitrogen source: from rich to limiting nitrogen conditions.

Integrated transcriptomic and metabolomic analyses reveal the mechanism of hypoxia tolerance in Aspergillus chevalieri under controlled nitrogen atmosphere.

Urolithin 9-dehydroxylase from Enterocloster bolteae JCM 12243T catalyzing regiospecific dehydroxylation of urolithins.