The management of transboundary aquifers across the Americas faces significant challenges, especially as climate change and population growth intensify groundwater stress. Groundwater use has increased to support domestic, industrial, and agricultural demands but has been extracted through unregulated withdrawals, leading to the severe degradation of aquifer health and transboundary frictions. This study focuses on how hydro-diplomacy can accelerate the adaptive governance of shared groundwater resources in three key regions: Canada–USA, USA–Mexico, and Mexico–Guatemala–Belize. We utilized a mixed methodology by integrating a transect approach, borrowed from ecology, into the field of geopolitics. To compare the hydro-diplomatic relations and groundwater governance across a continental gradient in the Americas, we conducted a literature review and employed the TWINS conflict–cooperation matrix to evaluate governance frameworks and hydro-diplomatic interactions across time. Our findings demonstrate that hydro-diplomacy plays a pivotal role in expediting agreements, fostering transboundary data sharing, and supporting participatory governance models. In particular, the presence of supranational bodies such as the International Joint Commission (IJC) between Canada and the USA has been effective in maintaining long-term collaboration through social learning and technical cooperation. Meanwhile, in regions like Mexico–Guatemala–Belize, the absence of robust institutions has hindered progress, with limited financial and knowledge-sharing networks. This study highlights the need for improved cross-border cooperation mechanisms and the establishment of common monitoring protocols to better manage aquifer resources under the pressures of climate change. The results support the development of more adaptive transboundary groundwater management strategies aligned with Sustainable Development Goal (SDG) 6.5.2 and call for broader geopolitical cooperation to address the complexities of groundwater governance.
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