Trans-sabinene Hydrate Research Articles

Influence of Biochar and Irrigation Levels on Productivity of Marjoram (Origanum majorana L.) under Sandy Soil Conditions

A field experiment designed as a split-plot experiment based on a randomized complete block design with three replications was conducted during two summer successive seasons 2021 and 2022 at the Experimental Farm of EL-Quassasin Horticultural Research Station, Ismailia Governorate; Egypt, to investigate the influence of biochar at rates of 0, 2, 4, and 6 tons/fed. at three irrigation levels (1, 1.5, and 2 hours/day) using a drip irrigation system on the growth, essential oil (productivity and constituents), as well as some chemical constituents of marjoram (Origanum majorana L.) plant grown in sandy soil. The obtained results revealed that both irrigation and biochar levels affected all the above-mentioned traits. It was clear that plant growth parameters increased as long as increasing irrigation levels and biochar rate. Considering the interaction, it was clear that generally the highest values in vegetative growth (plant height, number of branches, and herb fresh & dry weights/ plant), essential oil yield/plant, N%, and K% were attained from the treatment of irrigation at 1.5 hours/day combined with biochar at the rate of 6ton/fed. On the other hand, the highest values of essential oil%, essential oil components (p-cymene, trans-Sabinene hydrate, α-terpineol), as well as proline, were recorded in stressed plants (received 1h/ day irrigation and 0 biochar). The existing outcomes show that biochar application might increase water use efficiency and produce a high yield of marjoram. Subsequently, it could be a viable option to alleviate the severity effect of water deficit in marjoram growth, which opens opportunities for cultivating arid regions.

Phytochemical composition, in vitro bioactivity evaluation and in silico molecular docking study of fruit essential oils of Zanthoxylum armatum DC collected from Himalayan region of Uttarakhand, India

The present research aimed to analyze the nematicidal and insecticidal activity of the essential oil obtained from the fruits of Zanthoxylum armatum collected from Gumma village of Dharchula and Chandak village of Pithoragarh. After extraction via hydro distillation, the essential oils were subjected to GC–MS for their compositional analysis. A total of 10 and 13 components were identified for the Dharchula and Pithoragarh collection respectively. Linalool (32.86 %) was the major component in collection from Dharchula whereas it was totally absent in that of collection from Pithoragarh which has trans-sabinene hydrate (11.7 %) as its major component. Essential oils were tested against Meloidogyne incognita for their nematicidal activity and inhibition of egg hatching in laboratory conditions. It was found that the activity of Z. armatum from Dharchula shows high mortality of Meloidogyne incognita and more inhibition to the egg hatching. When tested for insecticidal activity against Lipaphis erysimi, collection from Dharchula presented stronger activity compared to Pithoragarh collection as shown by their LC50 values 0.006 and 0.165 µL/mL respectively after 36 hrs. The in silico PASS study and molecular docking study was conducted to screen the nematicidal and insecticidal potential of the major components on acetylcholinesterase receptors. Results showed good binding affinities and attributed the strongest inhibitory effect to verbenone for the target protein.

Microbiological Studies on the Influence of Essential Oils from Several Origanum Species on Respiratory Pathogens.

Essential oils (EOs) with established and well-known activities against human pathogens might become new therapeutics in multidrug-resistant bacterial infections, including respiratory tract infections. The aim of this study was to evaluate the antimicrobial activity of EOs obtained from several samples of Origanum vulgare, O. syriacum, and O. majorana cultivated in Poland. EOs were analyzed by GC-MS and tested against four bacterial strains: Staphylococcus aureus (MRSA), Haemophilus influenzae, Haemophilus parainfluenzae, and Pseudomonas aeruginosa. Chemical analyses showed that the Eos were characterized by a high diversity in composition. Based on the chemical data, four chemotypes of Origanum EOs were confirmed. These were carvacrol, terpineol/sabinene hydrate, caryophyllene oxide, and thymol chemotypes. Thin-layer chromatography-bioautography confirmed the presence of biologically active antibacterial components in all tested EOs. The highest number of active spots were found among EOs with cis-sabinene hydrate as the major compound. On the other hand, the largest spots of inhibition were characteristic to EOs of the carvacrol chemotype. Minimal inhibitory concentrations (MICs) were evaluated for the most active EOs: O. vulgare 'Hirtum', O. vulgare 'Margarita', O. vulgare 'Hot & Spicy', O. majorana, and O. syriacum (I) and (II); it was shown that both Haemophilus strains were the most sensitive with an MIC value of 0.15 mg/mL for all EOs. O. majorana EO was also the most active in the MIC assay and had the highest inhibitory rate in the anti-biofilm assay against all strains. The most characteristic components present in this EO were the trans-sabinene hydrate and terpinen-4-ol. The strain with the least sensitivity was the MRSA with an MIC of 0.6 mg/mL for all EOs except for O. majorana, where the MIC value reached 0.3 mg/mL. Scanning electron microscopy performed on the Haemophilus influenzae and Haemophilus parainfluenzae biofilms showed a visible decrease in the appearance of bacterial clusters under the influence of O. majorana EO.

Evaluating glufosinate-ammonium and flame weeding for weed control in sweet marjoram (Origanum majorana L.) cultivation

The objective of this four-year study was to compare the effects of flame weeding and spraying with glufosinate-ammonium on weed control in sweet marjoram field cultivation. The experiment was set up in a complete randomized four-replicate design with a plot area of 8 m2. Moreover, the content of essential oil in marjoram herb obtained from the control plants, as well as the composition of this oil were also studied. Marjoram emergence began 13–17 days after sowing, and it was very uneven and in some years it was extendend up to 5 weeks. Flaming (90 kg propane ∙ ha–1) was applied in two times: immediately after noticing the first marjoram emergence or 5 days later, while glufosinate-ammonium (600 g ∙ ha–1) was applied on the same date as the first flaming. The tested methods of weeding significantly reduced the number and fresh weight of weeds growing 3–4 weeks after the start of marjoram emergence, i.e. in the period of its greatest sensitivity to weed competition. They had no soil residual activity and their effect on weeds disappeared after the first weeding. Among weeds occurring in the experiment, only barnyardgrass (Echinochloa crus-galli (L.) P. Beauv.) was partially tolerant to flaming. Flaming applied at the second time did not increase the effectiveness of weed control, but significantly decreased the number of emergence and the yield of marjoram herb. Essential oil content in marjoram grated herb varied considerably depending on the year, ranging from 1.35 to 2.34%. The oil contained from 33 to 36 identified compounds, among which trans-sabinene hydrate (28–34%) and terpinen-4-ol (17–21%) were clearly dominant.

Phytochemical Analysis and Antioxidant, Antibacterial, and Antifungal Effects of Essential Oil of Black Caraway (Nigella sativa L.) Seeds against Drug-Resistant Clinically Pathogenic Microorganisms.

Nigella sativa (NS) is a plant that has long been utilized in traditional medicine as a treatment for certain diseases. The aim of this work was to valorize the essential oil (EO) of this species by phytochemical analysis and antimicrobial and antioxidant evaluation. EO was extracted by hydrodistillation from the seeds of Nigella sativa (EO-NS). Phytochemical content of EO-NS was evaluated by use of gas chromatography coupled to mass spectrometry (GC-MS/MS). Antioxidant ability was in vitro determined by use of three assays: 2.2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing power (FRAP), and total antioxidant capacity (TAC) relative to two synthetic antioxidants: BHT and quercetin. Antimicrobial effect was evaluated against four clinically important bacterial strains (Staphylococcus aureus, ATCC 6633; Escherichia coli, K12; Bacillus subtilis, DSM 6333; and Proteus mirabilis, ATCC 29906) and against four fungal strains (Candida albicans, ATCC 10231; Aspergillus niger, MTCC 282; Aspergillus flavus, MTCC 9606; and Fusarium oxysporum, MTCC 9913). Fifteen constituents that accounted for the majority of the mass of the EO-NS were identified and quantified by use of GC-MSMS. The main component was O-cymene (37.82%), followed by carvacrol (17.68%), α-pinene (10.09%), trans-sabinene hydrate (9.90%), and 4-terpineol (7.15%). EO-NS exhibited significant antioxidant activity with IC50, EC50, and total antioxidant capacity (TAC) of 0.017 ± 0.0002, 0.1196 ± 0.012, and 114.059 ± 0.97 mg EAA/g, respectively. Additionally, EO-NS exhibited promising antibacterial activity on all strains under investigation, especially on E. coli K12 resulting in inhibition diameter of 38.67 ± 0.58 mm and a minimum inhibitory concentration (MIC) of 1.34 ± 0.00 μg/mL. Also, EO-NS had significant antifungal efficacy, with a percentage of inhibition of 67.45 ± 2.31% and MIC of 2.69 ± 0.00 μg/mL against F. oxysporum, MTCC 9913 and with a diameter of inhibition 42 ± 0.00 mm and MIC of 0.67 ± 0.00 μg/mL against C. albicans. To minimize development of antibiotic-resistant bacteria, EO-NS can be utilized as a natural, alternative to synthetic antibiotics and antioxidants to treat free radicals implicated in microbial infection-related inflammatory reactions.

Phytochemical composition of essential oils from aerial parts of sweet basil infected by a mixed infection of tobacco mosaic virus and phytoplasma

Ocimum basilicum, commonly known as sweet basil, was found naturally infected showing mosaic mottling and witches’ broom symptoms. Semi-nested PCR using specific primers revealed the presence of a mixed infection of TMV and Candidatus phytoplasma. This study aims to evaluate and compare the phytochemical composition of essential oils from aerial parts produced in sweet basil. The comparison covered uninfected, mixed infected, TMV infected, and phytoplasma infected sweet basil. Thirty different essential oils were isolated and evaluated using gas chromatography-mass spectrometry (GC-MS) analysis. The essential oils produced were grouped into 5 main classes: monoterpenes hydrocarbons, oxygenated monoterpenes, sesquiterpenes hydrocarbons, oxygenated sesquiterpenes and ketones. All classes were severely altered due to TMV and Candidatus phytoplasma infection compared to the control. The pathological stress caused by the mixed infection was significantly higher than each pathogen separately. The monoterpenes hydrocarbons, oxygenated monoterpenes and ketones showed a significant increase in the essential oil percentages induced in the mixed infection than that of single infections. Yet, a significant decrease in sesquiterpenes hydrocarbons and oxygenated sesquiterpenes was reported in the infected plants. Furthermore, α-bergamotene, (+)spathulenol, α bergamotene, δ-guaiene, cubenol, bornyl acetate and 1,3-bis(cinnamoyloxymethyl)adamantane, were found only in healthy plants, while α-pinene, camphene, sabinene, β-pinene, β-myrcene, trans-sabinene hydrate, eugenol and trans-caryophyllene were detected only in infected plants. This study reports the possible metabolic consequences and defense mechanisms that induced in sweet basil due to TMV and phytoplasma infection.

GC/MS analysis and potential synergistic effect of mandarin and marjoram oils on Helicobacter pylori

Helicobacter pylori can cause chronic gastritis, peptic ulcer, and gastric carcinoma. This study compares chemical composition and anti-H. pylori activity of mandarin leaves and marjoram herb essential oils, and their combined oil. GC/MS analysis of mandarin oil revealed six compounds (100% identified), mainly methyl-N-methyl anthranilate (89.93%), and 13 compounds (93.52% identified) of marjoram oil, mainly trans-sabinene hydrate (36.11%), terpinen-4-ol (17.97%), linalyl acetate (9.18%), and caryophyllene oxide (8.25%)). Marjoram oil (MIC = 11.40 µg/mL) demonstrated higher activity than mandarin oil (MIC = 31.25 µg/mL). The combined oil showed a synergistic effect at MIC of 1.95 µg/mL (same as clarithromycin). In-silico molecular docking on H. pylori urease, CagA, pharmacokinetic and toxicity studies were performed on major compounds from both oils. The best scores were for caryophyllene oxide then linalyl acetate and methyl-N-methyl anthranilate. Compounds revealed high safety and desirable properties. The combined oil can be an excellent candidate to manage H. pylori.

Antimicrobial, Multidrug Resistance Reversal and Biofilm Formation Inhibitory Effect of Origanum majorana Extracts, Essential Oil and Monoterpenes.

Origanum majorana L. is a widely used medicinal plant; its distilled oil and preparations are extensively utilised in the phytotherapy and food industries. The objective of this study is to evaluate the extracts and the essential oil (EO) of Origanum majorana L, and its monoterpenes for antimicrobial, bacterial multidrug resistance reversing, and biofilm formation inhibitory potency. The composition of EO and n-hexane extract was characterized by GC-MS. In the essential oil terpinen-4-ol (24.92%), trans-sabinene hydrate (25.18%), γ-terpinene (6.48%), cis-sabinene hydrate (5.44%), p-cymene (4.72%), sabinene (4.53%), α-terpineol (4.43%), and α-terpinene (3.00%) were found as the main constituents while trans-sabinene hydrate (1.43%), and terpinen-4-ol (0.19%) were detected in the n-hexane extract besides a series of hydrocarbons. The antibacterial activity of EO and terpinen-4-ol, α-terpinene, and linalool was also assessed against sensitive and drug-resistant S. aureus, and E. coli strains with MIC values of 0.125–0.250% and 30–61 µM, respectively. In the efflux pump (EP) inhibitory assay, made by the ethidium bromide accumulation method in E. coli ATCC 25922, and AG100 and S. aureus ATCC 25923, and MRSA ATCC 43300 strains, EO exhibited substantial activity, especially in the E. coli ATCC 25922 strain. Among the EO constituents, only sabinene was an EP inhibitor in sensitive Escherichia strain. In the case of S. aureus strains, EO and sabinene hydrate exhibited moderate potency on the drug-resistant phenotype. The antibiofilm effects of the samples were tested by crystal violet staining at sub-MIC concentration. γ-Terpinene, terpinen-4-ol, sabinene, sabinene hydrate and linalool were found to be effective inhibitors of biofilm formation (inhibition 36–86%) on E. coli ATCC 25922 and S. aureus MRSA ATCC 43300, while EO was ineffective on these strains. In contrast to this, biofilms formed by E. coli AG100 and S. aureus ATCC 25923 were significantly inhibited by the EO; however, it was not affected by any of the monoterpenes. This observation suggests that the antibiofilm effect might be altered by the synergism between the components of the essential oil.

Effect on Yield and some Quality Characteristics of Seed Harvest at Different Stages of Maturity in Nigella sativa L.

Nigella sativa L. is a significant medicinal and aromatic plant due to the usage of both its seed and fixed oil. The aim of the study is to investigate the effects on seed yield, various yield components, fixed and essential oil content, and chemical composition (2018 year) of black cumin when harvesting the seed at four different stages of maturity. These stages were 25% (SH1), 50% (SH2), 75% (SH3) and 100% (SH4) browning of capsules. Two-year field experiments (2017 and 2018) were set up according to a randomized complete block design with triplicate, using a black cumin population obtained from the Burdur province under Isparta ecological conditions.Significant statistical differences were found among the harvest stages in regards to the seed yield, plant height and the thousand-seed weight, while no differences were found in the numbers of capsules and branches, essential oil and fixed oil contents in both experimental years. Seed yield and its components increased during the harvest stage up to SH3, while essential oil and fixed oil contents decreased insignificantly from SH1 to SH4 in both years. According to the combined years; plant height, the number of capsules, the number of branches, the 1000 seed weight, seed yields, essential oil and fixed oil contents varied between 38.3-42.5 cm, 6.03-6.85 capsule plant-1, 6.50-6.91 branches plant-1, 2.30-2.57 g, 307.3-542.3 kg ha-1, 0.087-0.101% and 31.14-32.69%, respectively. The main components of black cumin essential oil were characterized by cymol (25.01-26.90%), thymoquinone (2.39-4.41%), carvacrol (10.12-10.41%), junipene (5.33-6.66%), Δ-3-carene (5.55-8.71%), β-pinene (2.98-3.65), trans-sabinene hydrate (8.02-11.93%) and α-thujene (7.82-9.42%) according to harvest stages in the 2018 season. Considering the present results, SH3 stage was advised because of its higher seed yield. The contents of essential oil composition of black cumin varied according to harvest stages.

Antioxidant Activity and Molecular Docking Study of Volatile Constituents from Different Aromatic Lamiaceous Plants Cultivated in Madinah Monawara, Saudi Arabia.

A comparative study of volatile constituents, antioxidant activity, and molecular docking was conducted between essential oils from Mentha longifolia L., Mentha spicata L., and Origanum majorana L., widely cultivated in Madinah. The investigation of volatile oils extracted by hydrodistillation was performed using Gas Chromatography-Mass Spectrometry (GC-MS). A total number of 29, 42, and 29 components were identified in M. longifolia, M. spicata, and O. majorana representing, respectively, 95.91, 94.62, and 98.42, of the total oils. Pulegone (38.42%), 1,8-cineole (15.60%), menthone (13.20%), and isopulegone (9.81%) were the dominant compounds in M. longifolia oil; carvone (35.14%), limonene (27.11%), germacrene D (4.73%), and β-caryophyllene (3.02%) were dominant in M. spicata oil; terpin-4-ol (42.47%), trans-sabinene hydrate (8.52%), γ-terpinene (7.90%), α-terpineol (7.38%), linalool (6.35%), α-terpinene (5.42%), and cis-sabinene hydrate (3.14%) were dominant in O. majorana oil. The antioxidant activity, assessed using DPPH free radical–scavenging and ABTS assays, was found to be the highest in O. majorana volatile oil, followed by M. spicata and M. longifolia, which is consistent with the differences in total phenolic content and volatile constituents identified in investigated oils. In the same context, molecular docking of the main identified volatiles on NADPH oxidase showed a higher binding affinity for cis-verbenyl acetate, followed by β-elemene and linalool, compared to the control (dextromethorphan). These results prove significant antioxidant abilities of the investigated oils, which may be considered for further analyses concerning the control of oxidative stress, as well as for their use as possible antioxidant agents in the pharmaceutical industry.

Phytochemical Composition of Flower Essential Oil of Plumeria alba Grown in India

Plumeria alba (Family: Apocynaceae), commonly known as temple tree/white frangipani is an ornamental, deciduous, tropical plant grown in landscape places, gardens, parks, and home premises for its beautiful and fragrant flowers. The different parts of white frangipani are traditionally used for the treatment of several diseases. This study was executed to identify the volatile constituents of the flower essential oil of P. alba from India, as this has not been studied to date. The essential oil composition was investigated through GC/MS analysis. The isolation of essential oil through the hydrodistillation method produced a yield of 0.05±0.01 %. A total of 46 volatile constituents were identified, representing 98.11±0.5 % of the total essential oil. The essential oil was rich in esters comprising 48.61±0.3 % of the essential oil followed by oxygenated monoterpene (15.41±0.1 %), oxygenated sesquiterpene (10.43±0.08 %) and sesquiterpene hydrocarbons (10.39±0.1 %), etc. The dominant components of the essential oil were benzyl salicylate (33.98±0.04 %), benzyl benzoate (12.37±0.03 %), germacrene B (10.30±0.05 %), and linalool (8.17±0.11 %). Other notable constituents identified in the essential oil were (2Z,6Z)-farnesol, n-heneicosane, n-nonadecane, (Z, E)-geranyl linalool, trans-sabinene hydrate acetate, (2E,6Z)-farnesol, α-terpineol, n-docosane, geranyl benzoate, n-tricosane, α-bisabolol, nerol and 8-cedren-13-ol, etc. (1.17 % - 4.76 %).

Antifungal properties of volatile organic compounds produced by Daldinia eschscholtzii MFLUCC 19-0493 isolated from Barleria prionitis leaves against Colletotrichum acutatum and its post-harvest infections on strawberry fruits.

Fungal endophytes are microorganisms living symbiotically with a host plant. They can produce volatile organic compounds (VOCs) that have antimicrobial activity. This study aimed to isolate endophytic fungi from Barleria prionitis plants grown in Thailand and to investigate the antifungal properties of their VOCs against Colletotrichum acutatum, a causal agent of anthracnose disease on post-harvest strawberry fruits. A total of 34 endophytic fungi were isolated from leaves of B. prionitis. The VOCs produced from each individual isolate were screened for their antifungal activity against C. acutatum using a dual-culture plate method. From this in vitro screening experiment, the VOCs produced by the endophytic isolate BP11 were found to have the highest inhibition percentage (80.3%) against the mycelial growth of C. acutatum. The endophytic isolate BP11 was molecularly identified as Daldinia eschscholtzii MFLUCC 19-0493. This strain was then selected for an in vivo experiment. Results from the in vivo experiment indicated that the VOCs produced by D. eschscholtzii MFLUCC 19-0493 were able to inhibit infections by C. acutatum on organic fresh strawberry fruits with an average inhibition percentage of 72.4%. The quality of the pathogen-inoculated strawberry fruits treated with VOCs produced by D. eschscholtzii MFLUCC 19-0493 was evaluated. Their fruit firmness, total soluble solids, and pH were found to be similar to the untreated strawberry fruits. Solid phase microextraction-gas chromatographic-mass spectrometric analysis of the VOCs produced by D. eschscholtzii MFLUCC 19-0493 led to the detection and identification of 60 compounds. The major compounds were elemicin (23.8%), benzaldehyde dimethyl acetal (8.5%), ethyl sorbate (6.8%), methyl geranate (6.5%), trans-sabinene hydrate (5.4%), and 3,5-dimethyl-4-heptanone (5.1%). Each major compound was tested for its antifungal activity against C. acutatum using the in vitro assay. While all these selected VOCs showed varying degrees of antifungal activity, elemicin was found to possess the strongest antifungal activity. This work suggests that D. eschscholtzii MFLUCC 19-0493 could be a promising natural preservative for controlling C. acutatum associated anthracnose disease in strawberry fruits during the post-harvest period.

Detection of Alternaria alternata in tomato juice and fresh fruit by the production of its biomass, respiration, and volatile compounds

Tomato is widely consumed and marketed as juice, puree, or fresh product. Nevertheless, 30% of its harvest volume is lost because of the fungus Alternaria alternata. This research aimed to provide early detection methods for this fungal decay on tomato juice and fresh fruit. Biomass content, CO2, O2 and volatile compounds (VOCs) during A. alternata growth in tomato juice and fruit at two ripening stages (breaker and red colour) were evaluated. Additionally, CO2 and VOCs data set were analysed with a hierarchical cluster technique (HCA) to explore the differences between inoculated and non-inoculated samples. Biomass was determined by gravimetry, CO2 and O2 by gas chromatography (GC), and VOCs by GC-mass spectrometry. Biomass content was not drastically modified by tomato's ripening stage (3–6 mg of dry weight). CO2 in tomato juice was considerably higher in the inoculated samples with A. alternata (27–63%) than in the non-inoculated ones (2.8–6.6%), regardless of the ripeness stage; while in tomato fruit CO2 was higher at breaker stage and inoculated with A. alternata (33–41%) than the remaining treatments (9–23%). It was also observed that, except for limonene, trans-sabinene hydrate, and rhodovibrin, VOCs' release during the interaction between tomato juice and A. alternata was different from the fresh tomato and A. alternata interaction. Only the HCA based on CO2 data showed clear differences between the inoculated and non-inoculated tomato juice and fruit at both ripening stages.

Qualitative Variations of Lavandin Essential Oil under Various Storage Conditions

Essential oils (EOs) are widely used in products related to human health. Thus, providing an optimum storage condition to preserve their quality and stability is very important. The effects of various duration and storage conditions on lavandin (Lavandula × intermedia L.) EO was investigated as used the raw material of aromatherapy and health products. The watery and dehydrated samples were stored in laboratory temperature (24±2°C), refrigerator (+4°C) and freezer (-18°C) for 100 and 200 days. Also, a sample of watery and a sample of dehydrated EO were exposed to air at laboratory temperature. GC/FID-MS analyses were performed to determine the variations in the components of the stored EOs. Linalool (39.49-45.81 %) was the major component in all samples. The other main components were camphor (9.95-14.68 %), borneol (8.77-12.45 %), 4-terpineol (6.12-9.51 %), 1,8-cineole (1.27-6.79 %), linalyl acetate (3.45-4.02 %), cis-linalool oxide (0.30-4.39 %) and trans-linalool oxide (0.19-4.03 %). Due to the evaporation and conversion processes, most of the components had a decreasing or increasing tendency during the storage period. Also, components such as α-pinene, camphene, β-myrcene, γ-terpinene, α-terpinolene, trans-sabinene hydrate and D-carvone disappeared during the oxidation process. Based on observations, storage time and temperature, water presence, atmospheric gases and possibly light had destructive effects on the concentration of lavandin EO components. It can be estimated that the molecular weight, molecular structure and boiling point caused the oscillation of lavandin EO constituents under various storage conditions. According to the findings, the best storage conditions for lavandin EO were low temperature and the absence of atmospheric gases and water.

Influence of Salicylic Acid on Essential Oil Content and Changes its Compositions in Cuminum cyminum L.

The effect of foliar application of Salcylic acid (SA) on grain and oil yield of Cuminum cyminum L. in field condition were investigated in the region of Fasa (Fars province, Iran). Treatments comprised 0.1, 0.4, 0.7 and 1.1 mM SA applied to plants. Results indicated that the highest values of oil content (5.03 % v/w) and 1000 grain weight (3.20 g) were obtained from the application of 1.1 mM SA, however, no significant differences were found between SA levels on grain yield. The results of EO analysis showed that the amounts of β-pinene (2.36-3.48 %), ρ-cymene (7.55-9.19 %), γ-terpinene (9.61-12.37 %), γ-terpinene-7-al (24.47-31.96 %) and cumin aldehyde (24.60-29.19 %) as major oil components increased gradually with increasing SA levels, however, the amounts of cis-sabinene hydrate (0.04-0.06 %), trans-sabinene hydrate (0.12-0.17 %), terpinene-4-ol (0.16-0.28 %) and γ-terpinene-7-al (24.47-31.96 %) decreased with increasing SA levels.

Chemical Composition and Biological Activities of the Essential Oils of Leptospermum petersonii and Eucalyptus gunnii.

The aim of this study was to characterize the chemical composition and to evaluate the antimicrobial and phytotoxic properties of the essential oils (EOs) obtained from leaves of Leptospermum petersonii chemotype “Variety B” and Eucalyptus gunnii, native to Australia. Geranyl acetate, γ-terpinene, geraniol, terpinolene, α-pinene, p-cimene, and linalool were the main components in L. petersonii EO, confirming also the existence of several chemotypes in such taxa; on the other hand, 1,8-cineole, trans-sabinene hydrate acetate, globulol, longicyclene, terpinolene, and camphene were present in major amounts in the E. gunnii EO. Chemical analysis of L. petersonii revealed that it belongs to the variety “B.” E. gunnii EO showed good antibacterial activity, with an MIC of 0.5 and 2 μg/mL against Staphylococcus aureus, and Pectobacterium carotovorum, respectively. The activity of E. gunnii EO was stronger than L. petersonii EO, whose maximum MIC reached 5 μg/mL. E. gunnii and L. petersonii EOs were particularly effective in inhibiting the biofilm formation by S. aureus, already at a concentration of 0.01 μg/mL. The other strains were resistant to both EOs up to a dose of 0.05 μg/mL. The maximum inhibition on biofilm formed by P. carotovorum was recorded for E. gunnii EO, reaching a value of 93.12% at 1.0 μg/mL. This is the first manuscript which studies the biofilm inhibition by EOs and evaluates their effects on biofilm metabolism. Both EOs were more effective against P. carotovorum. In addition, even though L. petersonii EO 0.1 μg/mL was unable to inhibit biofilm formation by Escherichia coli, it decreased the metabolic activity of the biofilm to 78.55% compared to control; furthermore, despite it inducing a relatively low inhibition (66.67%) on biofilm formation, it markedly affected metabolic activity, which decreased to 16.09% with respect to the control. On the contrary, L. petersonii EO 0.5 μg/mL induced a 79.88% inhibition of S. aureus biofilm, maintaining a high metabolic activity (90.89%) compared to the control. Moreover, this EO showed inhibitory activity against radical elongation of Solanum lycopersicum and the germination of radish. On the contrary, E. gunnii EO showed no phytotoxic activity.

Kinetic studies of turpentine isomerization using hydrochloric acid and acetic acid as catalysts

Turpentine is a non-wood forest product derived from pine trees. Turpentine oil can be produced from the distillation of pine tree sap (family Pinaceae). The main component of turpentine is α-pinene, and turpentine isomerization may produce various derivative products. The objective of the present study was to investigate the isomerization products of turpentine oil with two acid catalysts: hydrochloric and acetic acid. Here, the influence of acid concentration was investigated by using various concentrations of hydrochloric and acetic acid concentrations between 0.4 to 1 M. Further, the combination of acetic acid and hydrochloric acid as catalyst was also investigated by varying the molar ratio of HCl:Acetic Acid=1 : 0.5 and 1:1. The experiments were carried out in a batch reactor equipped with heater, condenser, and stirrer. The reaction temperatures were maintained at 150°C and reaction time of 6 h. The resulting products from turpentine isomerization were analyzed using GC-MS. The results showed that the largest isomerization product obtained with HCl and combination of HCl:H.Acetic was trans sabinene hydrate. Additional products that could be detected varied among α-terpinolene, α-terpineol, α-terpinene, γ-terpinene and δ-carene. The experimental data showed that α-pinene conversion as high as 62, 63 and 57% were obtained by using HCl concentrations of 0.4, 0.7 and 1 M, respectively. In addition, the use of HCl:H.Acetic=1 :0.5 and 1:1 gave final conversion of 63 and 61%. The modeling results showed the kinetic model for pinene conversion for both catalysts was

Aroma Profile of Two Populations of Salvia verbenaca Collected from Two Bio-Geographical Zones from Jordan.

The aroma emitted from the different organs of two Salvia verbenaca L. populations from Jordan were extracted by Solid Phase Micro-Extraction (SPME) and then analyzed by GC/MS. The emission profile of the stem, leaf and sepal samples from the Mediterranean zone (Al-Salt) was dominated by monoterpene hydrocarbons (68.0 %, 33.7 %, and 42.2 %, respectively). The emission profile of flowering parts including pre-flowering buds, fully expanded flowers and petals was dominated by oxygenated monoterpenes (58.6 %, 59.3 % and 87.1 %, respectively). The major constituent detected in these organs was trans-sabinene hydrate acetate (range 14.5 %-87.0 %). On the other hand, samples collected from Irano-Turanian zone showed different emission patterns. While the stems, leaves and petal emissions were dominated by sesquiterpene hydrocarbons (54.9 %, 76.8 % and 52.6 %, respectively), monoterpene hydrocarbons dominated the emission profiles of the pre-flowering buds (75.1 %) and fully expanded flowers (73.6 %). Petals emissions were characterized by high concentrations of oxygenated monoterpenes (58.8 %). Notably, trans-sabinene hydrate dominated most organs emissions except for leaves (range 20.0 %-58.8 %). Principle Component Analysis (PCA) and Cluster Analysis (CA) revealed two different clusters related to the two different geographical zones. The current investigation revealed two ecotypes of S. verbenaca that could result in two different chemotypes. Trans-sabinene hydrate acetate and trans-sabinene hydrate are suggested compounds for identifying these two chemotypes.

Characterization of Chemical Components in Organs Emissions of Two Salvia dominica L. Populations from Jordan.

The current investigation reports the chemical composition of volatile organic compounds emitted from the different aerial organs of two populations of Salvia dominica L. from Jordan collected from Mediterranean and Irano-Turanian bio-geographical zones. Oxygenated monoterpenes dominated the emission profiles of most organs from the two populations but with variable qualitative and quantitative differences. Mediterranean samples contained higher content of oxygenated monoterpenes (stems: 88.37%, leaves 89.95%, pre-flowering buds 67.14%, fully opened flowers 79.43%, sepals 90.93% and petals 92.25%) as compared to those from Irano-Turanian origin (range 39.85% to 75.06%). trans-Sabinene hydrate dominated the emission profiles of all organs from Irano-Turanian zone (range 38.54% to 73.24%) in addition to the stem, sepal and petal samples from the Mediterranean zone (51.37% to 86.98%). The other organs from the Mediterranean zone were dominated by α-terpenyl acetate (27.66-54.87%). Cluster and Principle Component statistical analysis classified the two populations into two clusters based on their origin. The current study evidenced the different VOCs composition in the two populations, that was mainly related to climatic and environmental conditions and suggested the presence of two ecotypes of S. dominica L.in Jordan.

Thyme and suico essential oils: promising natural tools for potato common scab control.

Potato common scab is a worldwide disease mainly caused by Streptomyces scabiei. It seriously affects potato crops by decreasing tuber quality. Essential oils (EO) are natural products with recognised antimicrobial properties. In this research, the antibacterial activities of thyme, oregano, suico and mint EO against S. scabiei were analysed. Infected tubers and soil samples were used for bacterial isolation; the obtained isolates were genetically identified. The chemical composition of the EO was determined by GC-MS. The broth microdilution method was used to analyse antibacterial properties of EO. Thirty-one bacterial isolates were obtained. The isolate chosen for antibacterial assays was morpho-physiologically and genetically identified as S. scabiei. Thyme EO was mainly composed of thymol and o-cymene; suico EO of dihydrotagetone, trans-tagetone and verbenone; oregano EO of trans-sabinene hydrate, thymol and ɣ-terpinene; and mint EO of menthone and menthol. All the EO tested were effective against S. scabiei, but thyme and suico EO were the most successful, with a minimum inhibitory concentration of 0.068g·l-1 and 0.147g·l-1 , respectively, and a minimum bactericidal concentration of 0.137g·l-1 and 0.147g·l-1 , respectively. Scanning electron microscopy showed similar damage caused by both thyme and suico EO to the bacterial envelope. Total phenolic content of EO was not related to their antibacterial activity. Thyme and suico EO are effective antibacterial agents against S. scabiei, impeding bacterial viability and disturbing the bacterial cell envelope. These EO are promising tools for control of potato common scab.