With the rapid development of the social economy, the demand for talents is also constantly increasing, especially the demand for healthy physical fitness of college students. However, there are certain problems in the current curriculum of health and physical fitness training for college students, such as a lack of specificity and weak practicality. Therefore, this study is guided by professional characteristics and explores the construction and practice of health and physical fitness training courses for college students, in order to provide theoretical basis and practical reference for the reform of physical education courses in vocational colleges. This study conducted in-depth analysis of the physical fitness needs of different occupational groups through literature review, expert interviews, and other methods, in order to determine the goals and content of the health and physical fitness training course for college students. On this basis, a curriculum system for healthy physical fitness training for college students has been constructed, including aerobic exercise, strength training, flexibility training, balance training, and speed and agility training, among other aspects. This course system aims to comprehensively improve the health and physical fitness level of college students, enhance their career adaptation ability. A college student health and physical fitness training course guided by career characteristics can significantly improve the physical fitness level of college students, enhance their health status, and enhance their career adaptation ability. In addition, the curriculum system also has high practicality and pertinence, which can meet the needs of different occupational groups for health and physical fitness training. This study also puts forward some suggestions for the reform of physical education courses in vocational colleges. Strengthen the construction of the curriculum system, pay attention to the practicality and pertinence of the course content; Pay attention to the construction of the teaching team, improve the professional competence and teaching ability of teachers; Strengthen school enterprise cooperation, fully utilize enterprise resources, and improve the practicality of the curriculum. This study, guided by professional characteristics, explores the construction and practice of health and physical fitness training courses for college students, providing a certain theoretical basis and practical reference for the reform of physical education courses in vocational colleges. In future education and teaching work, we should continue to deepen curriculum reform, improve the quality of talent cultivation, and meet the demand of society for high-quality talents.