Trained Conditions Research Articles

Acute resistance exercise and training reduce desmin phosphorylation at serine 31 in human skeletal muscle, making the protein less prone to cleavage.

Desmin intermediate filaments play a crucial role in stress transmission and mechano-protection. The loss of its integrity triggers myofibril breakdown and muscle atrophy for which desmin phosphorylation (pDes) is a priming factor. We investigated whether eccentric accentuated resistance exercise (RE) influences the regulation of pDes, effecting its susceptibility to cleavage. Ten healthy persons performed 14 RE-sessions (2 per week). Muscle biopsies were collected in both untrained and trained conditions at rest (pre 1, pre 14) and one hour after RE (post 1, post 14). Western blotting and immunohistochemistry were utilized to assess desmin content, phosphorylation at several sites and susceptibility to cleavage. In untrained condition (pre 1, post 1), RE induced dephosphorylation of serin 31 and 60. Trained muscle exhibited more pronounced dephosphorylation at Serin 31 post-RE. Dephosphorylation was accompanied by reduced susceptibility of desmin to cleavage. Additionally, training increased total desmin content, upregulated baseline serine 31 phosphorylation and attenuated pDes at serine 60 and threonine 17. Our findings suggest that acute and repeated RE changes the phosphorylation pattern of desmin and its susceptibility to cleavage, highlighting pDes as an adaptive mechanism in skeletal muscle, contributing to the proteostatic regulation in response to recurring stress.

A Proposal for a Noxious Stimuli-Free, Moderate-Intensity Treadmill Running Protocol to Improve Aerobic Performance in Experimental Research on Rats.

Background/Objectives: Animal models can help understand human physiological responses, including the response to exercise and physical activity. However, many of these models incorporate noxious stimuli for various scientific purposes. We propose a noxious stimuli-free treadmill running training program for Rattus norvegicus species to study adaptations to aerobic exercise. Methods: In this study, rats were randomly allocated to training (n = 20) and sedentary (n = 20) groups. The training group underwent a program consisting of 30-50 min of treadmill running at 60% intensity, three times per week for 8 weeks. Maximum speed tasks (Tmax) were conducted to determine, adjust, and evaluate changes in fitness conditions. The rats had one week of familiarization with the treadmill, and a rubber ball was used at the back wall of the lane as a painless stimulus to encourage running. All assessments were conducted by two independent researchers in a double-blind manner, with data analysis conducted by a third-blind investigator. Results: A significant effect of time (η2p = 0.430, p < 0.001, large effect) could be found, showing differences between Tmax1 and Tmax2, and between Tmax1 and Tmax3 in both groups. The training group significantly outperformed the sedentary group (η2p = 0.266, p < 0.001, large effect). There was a significant interaction between time and condition (η2p = 0.152, p < 0.001, large effect). Conclusions: The proposed moderate-intensity treadmill running program could effectively differentiate between trained and sedentary conditions within both the short period of 4 weeks and the extended period of 8 weeks. This protocol can be used as a model for running on a treadmill for Rattus norvegicus species without the use of noxious stimuli.

IoT-Enabled Street Dustbin Monitoring System Based on Machine Learning

Abstract In this article, a novel method is proposed by using the power of the Internet of Things (IoT) and Machine Learning (ML) to monitor and manage street dustbins effectively. The proposed innovative solution aimed at optimizing waste management processes and improper disposing of the waste in street dustbins. The system involves the installation of sensors in dustbins, which collect real-time data about the fill level of the bins. The capability of the proposed model allows for dynamic adjustment of waste collection schedules, reducing unnecessary trips and thus saving time, energy, and resources. In addition to that, the model is also trained in various waste disposal scenario such as improper disposal, massive garbage dumps, and e-waste. The proposed model is able to predict and report the trained conditions with the 100% accuracy. The system is implemented using Raspberry Pi 4, a single-board computer with the necessary hardware interface. A suitable web interface has been developed to oversee the status of dustbins and provide optimum travel route for collection of waste from the street dustbins. The proposed system not only enhances operational efficiency but also contributes to environmental sustainability.

Detection of Signal Integrity Issues in Vibration Monitoring Using One-Class Support Vector Machine

AbstractThis paper presents an analysis of the common signal integrity issues in vibration monitoring caused by sensor saturation and signal distortion, or sensor loosening and detachment, and the development of a method of detecting the occurrence of vibration signal integrity issues using a one-class support vector machine. For this, vibration signals with distortions due to sensor saturation and/or sensor detachment are analysed to determine parameters sensitive to common integrity issues. These features are then extracted from training data of good quality vibration signals. Principal Component Analysis is used to dimensionally reduce the parameters which are then used in the training of the one-class support vector machine. The proposed method was validated on trained and untrained health conditions. Additionally, model sensitivity was also tested on trained health conditions with varying defect sizes. The method successfully detected all signal integrity issues tested proving to be an effective pre-processing step in condition monitoring systems for increased reliability and accuracy.

The Impact of Trained Conditions on the Generalization of Learning Gains Following Voice Discrimination Training.

Auditory training can lead to notable enhancements in specific tasks, but whether these improvements generalize to untrained tasks like speech-in-noise (SIN) recognition remains uncertain. This study examined how training conditions affect generalization. Fifty-five young adults were divided into "Trained-in-Quiet" (n = 15), "Trained-in-Noise" (n = 20), and "Control" (n = 20) groups. Participants completed two sessions. The first session involved an assessment of SIN recognition and voice discrimination (VD) with word or sentence stimuli, employing combined fundamental frequency (F0) + formant frequencies voice cues. Subsequently, only the trained groups proceeded to an interleaved training phase, encompassing six VD blocks with sentence stimuli, utilizing either F0-only or formant-only cues. The second session replicated the interleaved training for the trained groups, followed by a second assessment conducted by all three groups, identical to the first session. Results showed significant improvements in the trained task regardless of training conditions. However, VD training with a single cue did not enhance VD with both cues beyond control group improvements, suggesting limited generalization. Notably, the Trained-in-Noise group exhibited the most significant SIN recognition improvements posttraining, implying generalization across tasks that share similar acoustic conditions. Overall, findings suggest training conditions impact generalization by influencing processing levels associated with the trained task. Training in noisy conditions may prompt higher auditory and/or cognitive processing than training in quiet, potentially extending skills to tasks involving challenging listening conditions, such as SIN recognition. These insights hold significant theoretical and clinical implications, potentially advancing the development of effective auditory training protocols.

Resting Myokine Concentrations Are Reduced With Resistance-exercise Training But Blood Flow-restricted Resistance-exercise Response Is Unchanged.

Myokines are proteins and peptides produced and secreted by skeletal muscle; some are postulated to contribute to skeletal muscle hypertrophy. PURPOSE: To evaluate systemic myokine concentrations following acute blood-flow restricted resistance-exercise (BFR-RE) after a twelve-week traditional strength-training protocol in younger and older males. METHODS: Untrained males (younger: 24.7 ± 3.9 yrs, n = 8; older: 68.3 ± 4.6 yrs, n = 7) performed full-body BFR-RE before and after 12 weeks of progressive full-body strength training. At rest, immediately after, and 3, 6, 24 and 48 hours following each BFR-RE session, venous blood was collected and analyzed in duplicate to determine concentrations of interleukin-4 (IL-4), IL-6, IL-7, irisin, Leukemia Inhibitory Factor (LIF) and nitrate/nitrite. Area under the curve was calculated and a 2-way (age × training status) repeated measures ANOVA was conducted. RESULTS: For older males, IL-4 (untrained = 6996.9 ± 9260.2 AUC vs. trained = 5681.2 ± 8133.6 AUC; p = 0.038) and IL-6 (untrained = 819.2 ± 1104.5 AUC vs. trained = 636.5 ± 952.8 AUC; p = 0.016) were higher in the untrained condition than trained but did not differ for younger males. A significant age effect was identified for irisin, as younger males had higher concentrations than older (mean difference = 9423.8 ± 12546.8 AUC; p = 0.012). However, when AUC was calculated with baseline values subtracted, participants had higher IL-7 in the untrained condition compared to trained (mean difference = 33.5 ± 56.9 AUC; p = 0.04). Other myokines showed no training differences. CONCLUSION: Younger males had higher circulating irisin, and strength training decreased IL-4 and IL-6 in older males. The disappearance of age-related changes in IL-4 and IL-6 after baseline subtraction suggests those differences were due to lower baseline concentrations following the strength training program. Thus, IL-4 and IL-6 levels did not respond differently to BFR-RE in the untrained and trained conditions. Also, the lower response of IL-7 to BFR-RE in the trained vs. the untrained condition indicates that myokine secretion is affected by an individual’s level of training. Funding: This project was supported by grants (SMC & JEA) from the University Collaborative Research Program and Faculty of Science Collaborative - University of Manitoba.

General and Specific Aversive Modulation of Active Avoidance Require Central Amygdala.

Three studies provide evidence that the central nucleus of the amygdala, a structure with a well-established role in conditioned freezing, is also required for conditioned facilitation of instrumental avoidance in rats. First, the immediate early gene c-Fos was measured following the presentation of a previously shock-paired tone in subjects trained either on an unsignaled avoidance task or not (in addition to tone only presentations in naïve controls). Significantly elevated expression of c-Fos was found in both the avoidance trained and Pavlovian trained conditions relative to naïve controls (but with no difference between the two trained conditions). In a subsequent study, intracranial infusions of muscimol into the central amygdala significantly attenuated the facilitation of shock-avoidance by a shock-paired Pavlovian cue relative to pre-operative responding. The final study used a virogenetic approach to inhibit the central amygdala prior to testing. This treatment eliminated the transfer of motivational control over shock-avoidance by both a shock-paired Pavlovian stimulus, as well as a cue paired with a perceptually distinct aversive event (i.e., klaxon). These findings provide compelling support for a role of central amygdala in producing aversive Pavlovian-instrumental transfer.

Effects of Integrated Spelling in Phonics Instruction for At-Risk Children in Kindergarten

The purpose of the present study was to examine the effects of a brief experimental intervention that integrated spelling practice into a systematic phonics approach to initial reading instruction for at-risk children. The effects of this intervention were studied by means of a randomized controlled trial design that compared the experimental condition to two trained control conditions and a further business-as-usual condition. The two trained control conditions were phonics-based interventions without spelling but with additional time spent on letter-sound practice. One emphasized letter-sound production, the other letter-sound recognition. Participants were 65 kindergartners with limited letter knowledge and no reading skills. Each participant was randomly assigned to one of the four conditions. Children were taught individually in four 20-min sessions in all three trained conditions. Analyses of the gains in abilities from pre- to posttest revealed that the integrated spelling condition was associated with significantly larger gains in phoneme awareness, spelling, and reading than were either the trained letter-sound recognition condition (d = 0.38–0.86) or the business-as-usual condition (d = 0.54–1.21). The results also favored the integrated spelling condition over the trained letter-sound production condition. Regarding the two trained control conditions, the letter-sound production condition was associated with slightly better reading and spelling outcomes than the letter-sound recognition condition. These findings indicate that integrated spelling may improve systematic phonics for children at risk of early reading difficulties, and that activities that encourage letter-sound production may be more beneficial than those which only require letter-sound recognition.

Preschoolers benefit from a clear sound-referent mapping to acquire nonnative phonology

AbstractPrevious studies have shown that visual information is a crucial input in early language learning. In the present study we examine what type of visual input helps preschoolers in acquiring nonnative phonological contrasts. Catalan/Spanish-speaking children (4–5 years, N = 47) participated in a task to assess their phonological discrimination abilities before and after a training. Three training conditions were presented: one with clear oral/visual speech information, one with an ostensive object-sound mapping, and one with a rich social interaction. Children’s looking patterns were tracked to examine their focus of interest while being trained. Results revealed that preschoolers’ discrimination abilities increase in all trained conditions, but the condition where the speaker created an ostensive object–sound mapping led to higher long-term gains (especially for younger children). Eye-tracking results further showed that children looked to the object of reference while being exposed to the novel phonological input, which may explain the higher learning gains in this condition. Our results indicate that preschoolers’ learning of nonnative phonological contrasts is particularly boosted when the speech input is accompanied by an object of reference that is signaled ostensively and contingently in the visual space, compared to when the visual space only contains clear oral/visual speech information or social interactivity cues.

Is Innate Memory a Double-Edge Sword in Alzheimer's Disease? A Reappraisal of New Concepts and Old Data.

An emergent concept in immunology suggests that innate immune system is capable to undergo non-specific long-term responses and to provide resistance by modifying the reactivity to sequential pathogen challenge. This phenomenon, named innate memory, involves epigenetic, and metabolic reprogramming of innate immune cells. Current literature shows that the innate memory process has a mainly beneficial role in host defense, but sometimes can exert detrimental effects, as common in many diseases. Alzheimer's disease (AD) is a progressive neurodegenerative disorder characterized by cognitive decline and dementia. Accumulating findings demonstrate that inflammation is involved in AD pathogenesis and progression and recent genetic and functional data confirm the driving role of the innate immune component in the disease. Furthermore, AD patients show high burden of the most relevant infectious agents and up-regulation of inflammatory features in their innate immune cells, including an activated, or “primed” status of myeloid phagocytic cells in both brain and periphery, resembling trained immunity conditions. Thus, it is conceivable that AD innate cells may be firstly involved in the attempt to resolve recurrent/persistent inflammation but then acquire a trained phenotype mostly unable to maintain the immune regulation, leaving uncontrolled or sometimes supporting the progression of neurodegeneration. The present review aims to summarize evidence evoking innate immune memory mechanisms in AD, and to interpret their potential role, either protective or harmful, in disease progression. A better understanding of such mechanisms will provide a fertile ground for development of novel diagnostic, and therapeutic pathways in AD cure.

무오류 학습과 단계적 용암법을 활용한 맨드와 택트 훈련이 자폐 아동의 음성 언어 습득에 미치는 효과*

The purposes of this study were to explore what teaching strategy would be more effective in the speech acquisition for children with autism spectrum disorder, and to determine whether learned speech from the mand and tact training would be transferred to other verbal operant conditions. To investigate these research questions, 3 children with autism spectrum disorder participated in this study and alternating treatment designs were used to study the effects of independent variables including errorless learning and Systematic fading on the speech language acquisition. Results indicated that Systematic fading was more effective than Errorless learning for all participants to acquire verbal responses across the mand and tact training settings. Also, all three participants demonstrated skill transition for using target words from the trained verbal operant condition to other non trained verbal operant conditions. Discussion and implications on these findings would be addressed.

The short- and long-term effects of resistance training with different stability requirements.

The aim of this study was to assess the short- and long-term effects of resistance training (RT) with different stability requirements. Fifty-nine men underwent a 3-week familiarization period followed by a 7-week training period. During familiarization, all participants trained four sessions of squats with a Smith machine, free weights and free weights standing on a wobble board. After week-3, participants were randomized into a low (Smith machine), medium (Free-weight) or high (Wobble board) stability RT program, and Control group. All participants were tested pre-, after week-3 and post-intervention. Ten repetition maximum (10RM), rate of force development (RFD), electromyography (EMG) and maximum voluntary isometric contraction (MVIC) were tested in all three squat conditions in addition to countermovement jump (CMJ) on stable and unstable surfaces, and muscle thickness. After familiarization, greater 10RM loads (21.8–27.3%), MVIC (7.4–13.5%), RFD (29.7–43.8%) and CMJ (4.9–8.5%) were observed in all conditions. Between week 3 and 10, the Free-weight and Wobble board groups similarly improved 10RM in all conditions. Smith machine group demonstrated greater improvement in the trained exercise than the medium and high stability exercises. All training groups showed similar improvement in muscle thickness, RFD and MVIC. There was no CMJ improvement on the stable surface, but the Wobble board group demonstrated significantly greater improvement on the unstable surface. In conclusion, low, medium or high stability RT resulted in similar improvements in trained and non-trained testing conditions except for greater CMJ on the unstable surface in the Wobble group. Greater 10RM strength in trained than non-trained exercise was only observed in low stability group. Familiarization was associated with substantial improvements in 10RM and CMJ, with greater improvement associated with higher stability requirements. These findings suggest that high stability can increase strength, muscle thickness and explosive measurements similar to training with lower stability.

Technology or ecology? New tools to assess cognitive judgement bias in mice

Cognitive judgement bias tests have become important new tools for the assessment of animal emotions. They allow for the inference of an animal's emotional state based on ambiguous cue interpretations. As mice are the predominantly used animal model for cognitive and behavioural neuroscience, research in this field would considerably benefit from the development of suitable judgement bias tests for this species. Against this background, we aimed to implement two different active choice cognitive judgement bias paradigms for mice in a methodological study. For this purpose, two experiments were conducted: in experiment I, an automated, vision-based touchscreen technique was applied, allowing for the direct translation of tasks from rodents to humans and vice versa. Experiment II comprised a task relying on more ecologically relevant cues in form of tunnels of different lengths. While the touchscreen task was characterized by automation-related advantages such as the possibility to present many trials per session and a high convenience for the experimenter, the tunnel task was learned faster by the mice. In both tests, however, the response to the trained and ambiguous conditions resulted in a graded curve, the basic requirement for proving task validity. Thus, both the translational touchscreen task as well as the ecologically more relevant tunnel task could successfully be implemented and provide new tools for the future assessment of cognitive judgement biases in mice.

Limb-position robust classification of myoelectric signals for prosthesis control using sparse representations.

The fundamental objective in non-invasive myoelectric prosthesis control is to determine the user's intended movements from corresponding skin-surface recorded electromyographic (sEMG) activation signals as quickly and accurately as possible. Linear Discriminant Analysis (LDA) has emerged as the de facto standard for real-time movement classification due to its ease of use, calculation speed, and remarkable classification accuracy under controlled training conditions. However, performance of cluster-based methods like LDA for sEMG pattern recognition degrades significantly when real-world testing conditions do not resemble the trained conditions, limiting the utility of myoelectrically controlled prosthesis devices. We propose an enhanced classification method that is more robust to generic deviations from training conditions by constructing sparse representations of the input data dictionary comprised of sEMG time-frequency features. We apply our method in the context of upper-limb position changes to demonstrate pattern recognition robustness and improvement over LDA across discrete positions not explicitly trained. For single position training we report an accuracy improvement in untrained positions of 7.95%, p ≪ .001, in addition to significant accuracy improvements across all multiposition training conditions, p <; .001.

Effect of Different Exercise Intensities on the Myotendinous Junction Plasticity.

Myotendinous junctions (MTJs) are anatomical regions specialized in transmission of contractile strength from muscle to tendon and, for this reason, a common site where acute injuries occur during sport activities. In this work we investigated the influence of exercise intensity on MTJ plasticity, as well as on the expression of insulin-like growth factor 1 (IGF-1) and transforming growth factor beta (TGF-β) and their receptors in muscle and tendon. Three groups of rats were analyzed: control (CTRL), slow-runner (RUN-S) and fast-runner (RUN-F) trained using a treadmill. Ultrastructural and morphometric analyses of distal MTJs from extensor digitorum longus muscles have been performed. Contractile strength and hypertrophy were investigated by using in vivo tension recordings and muscle cross-sectional area (CSA) analysis, respectively. mRNA levels of PGC-1α, vinculin, IGF-1Ea and TGF-β have been quantified in muscle belly, while IGF-1Ea, TGF-β and their receptors in tendon. Morphometry revealed an increased MTJ complexity and interaction surface between tissues in trained rats according to training intensity. CSA analysis excluded hypertrophy among groups, while muscle strength was found significantly enhanced in exercised rats in comparison to controls. In muscle tissue, we highlighted an increased mRNA expression of PGC-1α and vinculin in both trained conditions and of TGF-β in RUN-F. In tendon, we mainly noted an enhancement of TGF-β mRNA expression only in RUN-F group and a raise of Betaglycan tendon receptor mRNA levels proportional to exercise intensity. In conclusion, MTJ plasticity appears to be related to exercise intensity and molecular analysis suggests a major role played by TGF-β.

Perceptual Learning at a Conceptual Level.

Training in object recognition can produce a learning effect that is applicable to new viewing conditions or even to new objects with different physical properties. However, perceptual learning has long been regarded as a low-level form of learning because of its specificity to the trained stimulus conditions. Here we demonstrate with new training tactics that visual perceptual learning is completely transferrable between distinct physical stimuli. This finding indicates that perceptual learning also operates at a conceptual level in a stimulus-invariant manner.

The Acquisitional Value of Recasts in Instructed Second Language Speech Learning: Teaching the Perception and Production of English /ɹ/ to Adult Japanese Learners

The current study investigated the impact of recasts together with form‐focused instruction (FFI) on the development of second language speech perception and production of English /ɹ/ by Japanese learners. Forty‐five learners were randomly assigned to three groups—FFI recasts, FFI only, and Control—and exposed to four hours of communicatively oriented lessons. Whereas many FFI activities including explicit instruction were embedded into the treatment in order for the experimental groups to notice and practice /ɹ/ in a meaningful discourse, an instructor provided recasts only to the FFI‐recast group in response to their mispronunciation of /ɹ/. Perception was measured using a two‐alternative forced choice identification task, while pronunciation performance was elicited using controlled and spontaneous production tests and assessed by 10 naïve native‐speaking listeners. According to the statistical comparisons, whereas the FFI‐only group attained perception and production improvement particularly under trained lexical conditions, the FFI‐recast group demonstrated similar but generalizable gains both in trained and untrained lexical contexts. The results indicate that (a) FFI itself impacts various domains of L2 speech learning processes (perception, controlled, and spontaneous production) and (b) recasts promote learners’ attentional shift away from lexical units as a whole to phonetic aspects of second language speech (i.e., vocabulary to sound learning).

Implante valvular aórtico transarterial: experiencia en Chile

Transcatheter aortic valve implantation (TAVI) is taking a leading role in the management of patients with severe aortic stenosis. Valve replacement surgery prolongs survival and is the technique of choice considering its historical background and long experience worldwide. Recently however, TAVI has positioned itself as the only standard therapy for symptomatic patients who are not candidates for surgery. To report the experience with this new technique comparing our results with those reported in the literature. Between May 2010 and December 2011,17 patients aged 81 ± 7.3 years (58.8% men with an Euro SCORE 29 ± 22.4%) underwent a TAVI. The median transvalvular gradient was 54 ± 15.7 mmHg. All patients received a CoreValve™. Technical success was 100%, with a post implant gradient of 6.29 ± 3.3 mmHg. Residual aortic regurgitation was observed in 94%, none greater than grade II. There were no complications at the vascular access site. One patient developed cardiac tamponade during the procedure. Permanent pacemaker implantation was required in 35.2%. Hospital mortality rate was 5.8%, a figure that remained unchanged at 30 days of follow-up. In high-risk patients with aortic stenosis, TAVI has a high success rate and a low rate of complications. Besides an appropriate patient selection, a trained multidisciplinary team and technical conditions to solve possible complications of the procedure are required.

Different Patterns of Perceptual Learning on Spectral Modulation Detection Between Older Hearing-Impaired and Younger Normal-Hearing Adults

Young adults with normal hearing (YNH) can improve their sensitivity to basic acoustic features with practice. However, it is not known to what extent the influence of the same training regimen differs between YNH listeners and older listeners with hearing impairment (OHI)--the largest population seeking treatment in audiology clinics. To examine this issue, we trained OHI listeners on a basic auditory task (spectral modulation detection) using a training regimen previously administered to YNH listeners (≈ 1 h/session for seven sessions on a single condition). For the trained conditions on which pretraining performance was not already at asymptote, the YNH listeners who received training learned more than matched controls who received none, but that learning did not generalize to any untrained spectral modulation frequency. In contrast, the OHI-trained listeners and controls learned similar amounts on the trained condition, implying no effect of the training itself. However, surprisingly the OHI-trained listeners improved over the training phase and on an untrained spectral modulation frequency. These population differences suggest that learning consolidated more slowly, and that training modified an aspect of processing that had broader tuning to spectral modulation frequency, in OHI than YNH listeners. More generally, these results demonstrate that conclusions about perceptual learning that come from examination of one population do not necessarily apply to another.

Direct Observation of Family Management: Validity and Reliability as a Function of Coder Ethnicity and Training

This study examines the influence of coder ethnicity on the validity and reliability of direct observations of family management. Eight coders, 4 European American (EA) and 4 African American (AA), were randomly assigned to conduct behavior ratings of videotaped family interactions of European American and African American families, under two conditions: untrained and trained. Results indicated statistical differences between EA and AA coder ratings of family management practices across both untrained and trained conditions, suggesting the presence of ethnocentric perceptions of coders. Specifically, EA coders tended to rate AA families as exhibiting poorer family management skills compared with those of EA families. AA coder ratings for EA and for AA families showed no statistical differences. Although not statistically significant, posttraining coding results indicated a trend toward decreased differences among coder perceptions, especially in improving the validity and reliability of EA coder ratings of AA families. These findings are discussed with respect to recommendations for cross-cultural research as well as general theories of ethnic socialization.