Landrace Research Articles

Population structure and selective signature analysis of local sheep breeds in Xinjiang, China based on high-density SNP chip.

The frigid and droughty climate of Xinjiang in China has given rise to unique indigenous sheep breeds with robust adaptability and resistance. To investigate the genetic mechanism of adaptability of Xinjiang sheep to the local extreme environment, we conducted population genetic structure analyses for three native Xinjiang sheep breeds: Altay sheep (ALT), Bashbay Sheep (BSBC), and Duolang sheep (DLC), as well as two foreign sheep breeds: Suffolk and Dorset, using the Ovine Infinium HD SNP BeadChip(680K). Our findings revealed distinct genetic and evolutionary histories between Xinjiang and foreign sheep breeds. Principal Component Analysis (PCA) and phylogenetic tree effectively differentiate these five sheep breeds based on their geographical origins, and the domestication level of Xinjiang sheep is comparatively lower than that of foreign sheep breeds. Furthermore, by utilizing three selective signature methods, namely Fixation Index (Fst), Cross Population Extended Haplotype Homozygosity Test (XP-EHH), and Nucleotide Diversity (π), we have successfully identified 22 potential candidate genes. Among these genes, there are TBXT, PDGFD, and VEGFA, which are closely related to tail type and lipid metabolism; VIL1, SLC11A1, and ZBTB46, which are associated with immune function; and candidate genes such as BNC1, HDAC1, and BMP5, which impact sheep reproductive traits. This study establishes a foundation for conserving and utilizing local sheep germplasm resources in Xinjiang and provides molecular insights into the genetic mechanisms governing sheep adaptation to extreme cold and arid environments.

Whole-genome sequencing reveals genetic structure and adaptive genes in Nepalese buffalo breeds.

Indigenous buffaloes, as the important livestock species contributing to economy of the country, are the lifeline of livelihood in Nepal. They are distributed across diverse geographical regions of the country and have adapted to various feeding, breeding, and management conditions. The larger group of these native buffalo breeds are present in narrow and stiff hilly terrains. Their dispersal indicates a possible environmental adaptation mechanism, which is crucial for the conservation of these breeds. We utilized whole-genome sequencing (WGS) to investigate the genetic diversity, population structure, and selection signatures of Nepalese indigenous buffaloes. We compared 66 whole-genome sequences with 118 publicly available sequences from six river and five swamp buffalo breeds. Genomic diversity parameters indicated genetic variability level in the Nepalese buffaloes comparable to those of Indian breeds, and population genetic structure revealed distinct geography-mediated genetic differentiation among these breeds. We used locus-specific branch length analysis (LSBL) for genome-wide scan, which revealed a list of potentially selected genes in Lime and Parkote breeds that inhabit the hilly region. A gene ontology (GO) analysis discovered that many GO terms were associated with cardiac function regulation. Furthermore, complementary analyses of local selection signatures, tissue expression profiles, and haplotype differences identified candidate genes, including KCNE1, CSF1R, and PDGFRB, related to the regulation of cardiac and pulmonary functions. This study is a comprehensive WGS-based genetic analysis of the native Nepalese buffalo breeds. Our study suggested that the Nepalese "hilly" buffaloes, especially the Lime and Parkote breeds, have undergone some characteristic genetic changes and evolved increased cardiac and pulmonary function for their adaptation to the steep hilly terrains of the country.

Genome-Wide Scan for Selective Sweeps Reveals Novel Loci Associated with Prolificacy in Iranian Sheep Breeds in Comparison with Highly Prolific Exotic Breed

Domestication and selection significantly changed phenotypic traits in modern domestic animals. To identify the genomic regions associated with prolificacy in this study, 837 ewes from three Iranian indigenous sheep breeds, consisting of Baluchi, Lori-Bakhtiari, and Zandi uniparous breeds, and one Greek highly prolific dairy sheep, namely Chios, were genotyped using OvineSNP50K arrays. Statistical tests were then performed using different and complementary methods based on either site frequency (FST) and haplotype (hapFLK) between populations, followed by a pathway analysis of the genes contained in the selected regions. The results revealed that for the top 0.01 percentile of the obtained FST values, 16 genomic regions on chromosomes 2, 3, 4, 7, 8, 9, 13, 14, 16, 18, 19, and 20, and for hapFLK values, 3 regions located on chromosomes 3, 7, and 13, were under selection. A bioinformatic analysis of these genomic regions showed that these loci overlapped with potential candidate genes associated with prolificacy in sheep including GNAQ, COL5A2, COL3A1, HECW1, FBN1, COMMD3, RYR1, CCL28, SERPINA14, and HSPA2. These regions also overlapped with some quantitative trait loci (QTLs) linked to prolificacy traits, milk yield, and body weight. These findings suggest that future research could further link these genomic regions to prolificacy traits in sheep.

Comparative ultrastructural and transcriptomic profile analysis of skin tissues from indigenous, improved meat, and dairy goat breeds.

High-quality goatskins are valuable byproducts usually produced by indigenous goat breeds with poorer production performance in Asia and Africa. However, the genetic and molecular mechanisms underpinning goatskin's biomechanical properties (e.g., tensile strength) remain elusive. Mechanistic exploration of these traits could greatly aid the genetic improvement and genetic resource conservation of native breeds in these regions. To fulfill this purpose, we collected skin tissues from three goat breeds: Huai goat (HG), a Chinese native variety producing high-quality goatskins; Yudong meat goat (YDMG), a crossbreed of HG and Boer goat; Henan dairy goat (HNDG), a dairy goat breed. Scanning electronic microscopy analysis of skin tissues found that the collagen fiber diameters, collagen fibril diameters, and crimps significantly differed among the three goat breeds; however, collagen fibril diameters are similar in HG and HNDG. A sum of 230, 775, and 86 differentially expressed genes (DEGs) were identified from YDMG versus HNDG, HG versus HNDG, and YDMG versus HG, respectively. Functional enrichment analysis suggested that signaling pathways involved in fatty acid, retinol, steroid metabolisms, and GO items related to the physical properties of the skin (e.g., collagen-containing extracellular matrix) are significantly overrepresented in DEGs identified from meat versus dairy goats. Furthermore, 106 DEGs (e.g., COL1A1, COL1A2, and SPARC) showed specific expression patterns in HG and YDMG versus HNDG. Items about biophysical features of skin (e.g., extracellular matrix organization and ECM proteoglycans) are markedly enriched. Protein-protein interaction analysis suggested that two growth factors (IGF1 and PDGFD) are latent collagen and other ECM protein expression modulators. Ultrastructural analysis of goat skin tissues suggested that collagen fibril diameter is not a major factor affecting goatskin quality. Transcriptomic profiles unveiled core genes and associated biological processes potentially involved in regulating goatskin quality. These discoveries shined new light on deeper understanding the mechanisms of hide-related traits in goat and other livestock.

Inference of Genetic Diversity, Population Structure, and Selection Signatures in Xiangxi White Buffalo of China Through Whole-Genome Resequencing

(1) Background: Buffaloes are crucial livestock species for food and service in tropical and subtropical regions. Buffalo genetics, particularly in indigenous Chinese breeds such as the Xiangxi white buffalo (XWB), remains an intriguing area of study due to its unique traits and regional significance. (2) Methods: This investigation utilized the whole-genome sequences of twenty XWBs (newly sequenced), along with eighty published whole-genome sequences of other buffalo breeds (including Guizhou white buffalo, river buffalo, and Chinese buffalo in the Yangtze River). Using whole-genome sequencing analysis technology, the population structure, genomic diversity, and selection signatures of XWB were determined. (3) Results: This study revealed that the XWB, being phylogenetically positioned in the middle and lower reaches of the Yangtze River, exhibited substantial genomic diversity. Employing four selection sweep detection methods (CLR, iHS, π-ratio, and FST), several genes were positively identified for adaptive traits in the XWB, including coat color phenotypes (ASIP, KIT), the nervous system (GRIK2), reproduction (KCNIP4), growth and development (IFNAR1, BMP6, HDAC9, MGAT4C, and SLC30A9), the body (LINGO2, LYN, and FLI1), immunity (IRAK3 and MZB1), and lactation (TP63, LPIN1, SAE1). (4) Conclusions: In conclusion, this study enhances our understanding of the genetic distinctiveness and adaptive traits of XWB, highlighting selection signatures crucial for future breeding and conservation and ensuring sustainable use of this vital livestock resource.

Analysis of inter-varietal hybridization in rice (Oryza sativa L.) indicate transgressive segregation

Traits under continuous stabilizing selection may yield transgressive phenotypes in suitable cross combinations. Farmers maintained Njavara (Oryza sativa L. landrace Njavara), a poor yielding medicinal rice landrace in Kerala state over centuries for medicinal applications using short duration (60-70 days) as a selection criterion. This implies that the heading date together with several of the yield related traits, which are co-controlled by pleiotropism, were under constant stabilizing selection in Njavara. We examined the level of transgression in 13 yield related traits in F2 populations raised from crosses between Njavara and a high yielding hybrid variety Jyothi. The cross yielded transgressive phenotype in all of the 13 traits in F2 populations. Difference in the genetic architecture between Njavara and Jyothi due to the difference in the selection pressure experienced by them may have contributed to the excessive transgression observed in the study. The results highlight the value of unattractive poor yielding land races in providing alleles to lift yield barrier in high yielders, and suggest the need to examine the genetic architecture of the trait of interest in parental lines for better genetic advancement in crop improvement programmes.

Assembly and characterization of the complete chloroplast genome of the Colombian coffee varieties Caturra Chiroso, Bourbon Chiroso and Chiroso, Coffea arabica L. (Rubiaceae), with insights on their phylogenetic relationships

AbstractVarieties represent a defined group with differentiated characteristics derived through natural selection and/or selective breeding from within a species. In the Central-Andean region of Colombia (Urrao) there are three endemic varieties of the species Coffea arabica L [“Caturra Chiroso” (CCH), “Bourbon Chiroso” (BCH), and “Chiroso” (CHCH)], known as “Chiroso” group, globally renowned for their high quality and distinctive cup profile. Despite its significance, there is a lack of reported genomic resources or basic biological information for these. In this study, we conducted the first assembly and characterization of the complete chloroplast (Cp) genomes of these varieties and reconstructed their ancestry relationships. The Cp genomes were 155,188 bp in length (A = 30.93%; C = 19.06%; G = 18.37%; T = 31.64%); containing 131 genes, comprising 86 protein-coding genes, 8 rRNA genes, and 37 tRNA genes. They consisted of four subregions: the large single-copy (LSC) region (85,159 bp; 83 genes), the short single-copy (SSC) region (18,136 bp; 12 genes), and the inverted repeats IRA (25,944 bp; 18 genes) and IRB (25,945 bp; 18 genes). Likewise, among 26 intraspecific varieties analyzed, CCH + BCH formed a unique haplotype, and CHCH + Bourbon + Caturra formed another. CCH and BCH featured an exclusive Cytosine mutation (SNP: C/A), position 47,413 bp (intergenic spacer region trnT(UGU)-trnL(UAA)]. Likewise, a total of 445 short tandem repeats were found in the Cp genomes (dinucleotides: 370; trinucleotides: 71; tetranucleotides: 1). Finally, the three formed a well-supported monophyletic group with conspecific varieties, being more closely related to Eastern Ethiopian-origin varieties [e.g. Berbere region], as well as with traditional ones like Typica, Bourbon, and Caturra. These coffee varieties are a valuable new genetic resource for use as a gene source for genetic improvement, biotechnology, direct exploitation and cultivation worldwide.

Importance of Proper Breeding Techniques in Enhancing Magur Seed Production

Fish farmers in eastern India, consider it to be a species of choice for diversifying their cultural practice since it provides them with a considerable return on their investment. The natural seeds of Magur have been sharply declining due to ecological imbalance in their natural breeding ground, which is low lying paddy field. This imbalance is caused by the indiscriminate use of chemical fertilizer and chemical pesticides. However, the availability of Magur seeds in desired hours of stocking in desired quantity stands as a constraint to the propagation of culture of this species. It is consequently the only method that is feasible to reduce the severity of the issue, and that is to induce breeding of the species in captivity. There have only been a few models designed for the purpose of reproducing Magur in captivity, but each of these models involves a significant amount of financial investment. However, a labor-intensive and low-cost farmers'-friendly model for the production of small quantity seeds has not yet been standardized for rural small scale fish farmers. In this paper we found that magur seed production can be done through low cost investing, that’s why farmers or rural people get more economic profit. The study demonstrates how specific breeding techniques and management practices can significantly enhance seed production, contributing to the sustainability of Magur aquaculture in India.

The genome landscape of the Xinglong buffalo

BackgroundXinglong buffalo, as an indigenous breed in Hainan province of China, possesses characteristics such as high humidity tolerance, disease resistance and high reproductive capacity. Combined with whole genome sequencing technology, comprehensive investigation can be undertaken to elucidate the genomic characteristics, functions and genetic variation of Xinglong buffalo population.ResultsXinglong buffalo has the highest genetic diversity, lowest runs of homozygosity average length, and fasted decay of linkage disequilibrium in our study population. Phylogenetic tree results revealed that Xinglong buffalo was gathered together with Fuzhong buffalo firstly. The population genetic structure analysis indicates that at K = 3, the Xinglong buffalo for the first time showed a distinct ancestral origin from other water buffalo. Furthermore, compared to different populations, candidate genes displaying significantly distinct patterns of single nucleotide polymorphisms (SNPs) (e.g., RYR2, COX15, PCDH9, DTWD2, FCRL5) distribution have been identified in the Xinglong buffalo.ConclusionsBased on the whole genome sequencing data, this study identified a substantial number of SNPs and assessed the genetic diversity and selection signatures within the Xinglong buffalo population. These results contribute to understanding the genomic characteristics of Xinglong buffalo and their genetic evolutionary status. However, the practical significance of these signatures for genetic enhancement still requires confirmation through additional samples and further experimental validation.

Indigenous Technical Knowledge and Its Potential Application to Uganda's Pig Sub‐Sector

ABSTRACTUganda's pork consumption is speculated to exceed any other livestock meat consumption by 2050. However, the current trend in pig production may not meet the demand due to pig production constraints such as recurrent disease incidences and high feeding costs. The proposed modern practices are not cost‐effective, especially for resource‐constrained pig farmers. This review, therefore, synthesises previous studies on the practicability of integrating Indigenous Technical Knowledge (ITK) in pig production to mitigate the prevailing pig production challenges in Uganda. By employing ITK, smallholder farmers have realised that indigenous pig breeds are disease‐resistant and suit the prevailing harsh tropical conditions. In addition, affordable plant‐based concoctions have been developed to cure diseases in pigs. For example, Centella asiatica has been identified as a potential purgative for diarrhoeal infections. Integrating pigs in cropping systems has also been adopted as a low‐cost alternative to effectively feed pigs for optimal performance. Fortunately, most of the ITK options for solving pig production challenges are feasible in Uganda. Thus, ITK should be embedded in the country's extension system to increase smallholder farmers' awareness about its potential of improving pig production. An ITK‐modern technology integration would probably meet the acceptance and be suitable for commercial pig producers.

Volcanic Terroirs: Exploring Minerals in Canary Red Wine

The mineral composition of monovarietal red wines from the Canary Islands was analyzed to evaluate the potential of mineral content as a marker for wine authenticity by geographical origin. Key minerals—K, Na, Mg, Mn, Fe, Cu, and Co—were quantified in 190 wine samples using flame absorption spectrometry. The study revealed slight mineral profile differences between recently introduced international grape cultivars and traditional ungrafted varieties. A significant correlation was found between K and Mg, highlighting their roles in vine physiology. The results indicated that Tenerife wines had elevated Fe and Mn, Lanzarote wines showed higher Na (likely from marine aerosols), and La Gomera wines had significantly high Mn. Linear discriminant analysis demonstrated that Mn, Mg, and Na differentiated wines by island with 85% classification accuracy, while Cu and Fe correlated with wine ageing. These findings emphasize the influence of volcanic soils and microclimate on mineral profiles, supporting mineral analysis as a cost-effective tool for classifying red wines by origin. This study offers insights into how terroir, grape cultivar, and winemaking practises define the unique characteristics of Canary Island wines.

A comparative study on semen quality and cryopreservation ability in Italian native chicken breeds

This study provides a comparative assessment of fresh semen quality and sensitivity to cryopreservation in several Italian chicken breeds. The research involved 145 roosters from 13 breeds. The results showed a wide variability in all the parameters considered among the different breeds, especially in the quantitative variables of fresh semen, such as volume and concentration. For the qualitative characteristics (sperm membrane integrity and motility parameters), the variability across breeds was more pronounced for frozen than fresh semen. Interestingly, apart from total motility in fresh semen, breed had a significant effect on all semen quality parameters in both fresh and thawed ejaculates. Considering the overall qualitative characteristics, the Robusta maculata, Siciliana, and Mericanel della Brianza breeds produced ejaculates with better semen quality compared to other Italian breeds. By evaluating the main parameters of semen quality, our results underline the potential of these traits to influence the reproductive success and genetic conservation. The Bionda piemontese, Bianca di Saluzzo, Livorno bianca, Pepoi, and Siciliana breeds showed better resilience to cryopreservation, suggesting the need for breed-specific protocols to optimise semen quality after thawing. Importantly, the research highlights the central role of semen quality for both immediate fertilisation success and long-term conservation efforts. Future studies integrating OMICS technologies could elucidate molecular markers influencing breed-specific differences, helping to refine cryopreservation techniques and improve conservation strategies for indigenous Italian chicken breeds. This work contributes valuable insights to global efforts aimed at safeguarding poultry genetic diversity and sustainability.

Agrobiodiversity and food security: challenges and sustainable solutions

Objective: Agricultural biodiversity, also known as agrobiodiversity, encompasses the variety of plants, animals, and microorganisms that are directly or indirectly involved in agriculture. This diversity is the result of millennia of selection, management, and domestication of species, which has allowed societies to adapt to different environmental and cultural conditions. However, in recent decades, the loss of agrobiodiversity, accelerated by industrial agriculture, the expansion of monocultures, and the reduction of varieties, has put global food security at risk. This diversity is crucial to ensuring the resilience of agricultural systems in the face of challenges such as climate change, emerging pests, and the depletion of natural resources. This paper examines the importance of conserving agricultural biodiversity from the perspective of food security. It emphasizes how agrobiodiversity not only contributes to the stability of food production but also improves human diets by diversifying crops and providing essential micronutrients. Design/Methodology/Approach: A search was conducted on the following scientific information platforms: Web of Science database and Google Scholar. A systematic search for publications related to agrobiodiversity systems was carried out in the WoS database and Google Scholar over the last 49 years (1975–2024). Results: Genetic erosion is particularly concerning because genetic diversity is essential for crops to face environmental challenges such as climate change, pests, and diseases. The loss of traditional varieties, which are selected by local farmers to adapt to specific conditions, increases agriculture's vulnerability to external disruptions. These landraces, having been cultivated in genetically diverse mosaics, offer protection against catastrophic losses in the event of crop failures due to extreme conditions or diseases. Findings/Conclusions: Genetic diversity allows for the development of sustainable solutions to pests and diseases, reducing dependence on pesticides and promoting more environmentally friendly farming practices. However, challenges related to biodiversity conservation persist, making it essential to implement public policies that promote agrobiodiversity and address the socioeconomic issues that limit its adoption.

Genetic improvement of indigenous cattle breeds in Ethiopia: A systematic review of the Fogera cattle open nucleus breeding scheme

Fogera cattle are one of the valuable indigenous milk-type local breeds of Ethiopia, widely adapted to the area around Lake Tana in the Amhara region. The objective of this systematic review was to evaluate the performance of the Fogera cattle breed under an open nucleus breeding scheme. The review was done systematically by collecting published and unpublished data sources on the breed. The overall milk yield of the nucleus Fogera cattle herd was 2.26±0.794L/day. From the total herd, the top 10% and 25% of them produced daily milk yields of 3.31 and 2.87L, respectively, and some elite cows gave an average of 5.45±0.73L/day with a maximum yield of 8L/day. The predicted 305-day milk yield for the top 10% and 25% of the total herd was 883.64 and 772.83L, respectively. The average lactation milk yield and lactation length were reported to be 489±184L and 243±72.79 days, respectively. The respective heritability estimates for the aforementioned traits were 0.20±0.23 and 0.27±0.001. The birth and weaning weights (at 8 months of age) of village Fogera cattle born from community-based breeding programmes (CBBP) were 23.77±.21 and 85.89±1.07kg, respectively. The average weaning age for the CBBP herds was reduced to 8 months. The overall calf mortality in the nucleus herd was 3%. The CBBP demonstrated that it could act as a significant entry point for ensuring the conservation and restocking efforts of this breed as a country asset.

Beyond soybean meal: investigating the effects of dietary protein alternatives on gut health, liver function and microbiota in traditional slow-growing chicken breeds

The use of soybean meal in animal feed is crucial for nutrition but has a significant environmental impact, especially related to deforestation and biodiversity loss. With the increasing demand for sustainable agricultural practices, it becomes essential to explore alternatives to soybean meal. This study evaluated the effect of a soy-free diet, replaced with fava beans and pea protein, on an Italian slow-growing chicken breed: the Bianca di Saluzzo. The results indicate that the experimental diet did not compromise the growth, final weight or intestinal health of the animals, demonstrating a good tolerance and adaptability of the breed to these alternative ingredients. Furthermore, the analysis of the intestinal microbiota showed a positive impact, with an increase in organic acids such as succinic and citric, which could improve intestinal health and metabolic efficiency. Some changes in liver enzyme levels were observed, such as the increase in glutamate-oxaloacetate transaminase, an enzyme involved in amino acid metabolism. This increase could indicate a higher efficiency in protein metabolism, suggesting that the diet based on alternative ingredients could support an improvement in liver metabolism. Although this aspect deserves further investigation, it could represent a positive effect of the diet on liver function and overall health of the animals. Further studies are needed to fully understand the long-term implications of these dietary modifications, particularly in relation to the gut microbiota and metabolism.

Hypoxia stress: plant's sensing, responses, and tolerance mechanisms.

Oxygen (O2) is an inhibiting factor for plant growth and development in submerged and flooding environments. Plants experience different O2 concentrations, such as normoxia, hypoxia, and anoxia, which can change over space and time. Plants have evolved various morphological, physiological, and biochemical adaptations to withstand low O2 stress, many of which have been well investigated. This review provides a detailed analysis of how plants respond to hypoxia, a significant stress factor primarily caused by flooding. Hypoxia affects plants at various cellular, developmental, and environmental levels. This review highlights genetic, molecular, and metabolic adaptations crops employ to cope with O2 deficiency. The roles of various transcription factors (TFs) and gene regulation mechanisms in enabling plants to modulate their physiological responses under hypoxic conditions are notable. The review also identifies a significant gap in research on plant responses during reoxygenation, the phase of returning to normal O2 levels, especially under natural lighting conditions. This transition poses ROS generation and photoinhibition challenges, affecting plant recovery post-hypoxia. We discuss various strategies to enhance plant hypoxia tolerance, including traditional breeding, genetic modification, and grafting techniques. It emphasizes integrating these approaches with a comprehensive understanding of hypoxia sensing and response mechanisms. We underscore the complexity of plant adaptations to hypoxia and the need for continued research in this field, especially in the face of global climate change. This is vital for developing sustainable agricultural practices and ensuring future food security.

Applications of CRISPR Technologies in Forestry and Molecular Wood Biotechnology.

Forests worldwide are under increasing pressure from climate change and emerging diseases, threatening their vital ecological and economic roles. Traditional breeding approaches, while valuable, are inherently slow and limited by the long generation times and existing genetic variation of trees. CRISPR technologies offer a transformative solution, enabling precise and efficient genome editing to accelerate the development of climate-resilient and productive forests. This review provides a comprehensive overview of CRISPR applications in forestry, exploring its potential for enhancing disease resistance, improving abiotic stress tolerance, modifying wood properties, and accelerating growth. We discuss the mechanisms and applications of various CRISPR systems, including base editing, prime editing, and multiplexing strategies. Additionally, we highlight recent advances in overcoming key challenges such as reagent delivery and plant regeneration, which are crucial for successful implementation of CRISPR in trees. We also delve into the potential and ethical considerations of using CRISPR gene drive for population-level genetic alterations, as well as the importance of genetic containment strategies for mitigating risks. This review emphasizes the need for continued research, technological advancements, extensive long-term field trials, public engagement, and responsible innovation to fully harness the power of CRISPR for shaping a sustainable future for forests.

Isolation, Molecular Characterisation, and Pathogenicity Analysis of a Novel Recombinant ALV-J Strain Isolated From Chinese Hetian Chickens.

Avian leukosis virus subgroup J (ALV-J) primarily affects poultry, particularly chickens, leading to tumourigenesis and immunosuppression, which results in substantial economic losses. It is important to note that ALV-J is commonly found in indigenous chicken breeds in China, and the virus's vertical transmission characteristics present a significant threat to the preservation of local chicken breeds. The study aimed to investigate the characteristics and effects of the recombinant ALV-J strain LY2021J, with a focus on its genetic composition and its potential influence on virulence and pathogenicity. LY2021J was isolated using DF-1 cells and validated by enzyme-linked immunosorbent assay (ELISA) and IFA. The proviral genome was amplified using segmented PCR and then spliced together using DNASTAR software. Genome-wide genes, including gag, pol, gp85, and long terminal repeat (LTR), were compared. Recombination sites were analysed using RDP5 and SimPlot software. Pathogenicity was evaluated by monitoring symptoms and conducting examinations on SPF chickens. The outbreak of ALV-J in China has caused significant economic losses in the poultry industry. Although largely controlled in white-feather broilers and egg-laying chickens, ALV-J has spread to yellow-feather broilers and local breeds. A strain, LY2021J, isolated from Hetian chickens, showed lower mortality despite severe dysplasia. Genetic analysis revealed high similarity between LY2021J and the Chinese strains JS14NT01 and NX0101, suggesting a shared origin. Recombination with strain ev-1 and specific 3' UTR deletions may explain LY2021J's reduced virulence. Continued monitoring and prevention strategies are essential to mitigate ALV-J's impact.

Assessment of Reproductive Performance and Husbandry Practices of Horro and Crossbred Cattle Under Smallholder in Sibu Sire District of East Wollega Zone, Oromia, Ethiopia.

The reproductive and production performance of dairy cows determines the profitability of a dairy farm. A questionnaire survey was used to assess reproductive performance and husbandry practices by using 100 randomly selected animal owners. Seventy-nine percent of animal owners involved were men, ages between 30 and 60. Their education was as follows: 26% were illiterate, 67% in primary school and 7% in secondary school. Ninety-five percent of them managed their animals extensively and used natural mating and breeding practices (75%). AFS in months were 46.83±0.65 in Ada-Buke Warego, 46.00±1.36 in Hagalo-Tulam, 47.08±0.99 in Dicho Aba-Garmama and 47.29±0.51 in Waligalte. AFC in months were 56.68±0.97 in Ada-Buke Warego, 58.00±1.36 in Hagalo-Tulam, 57.23±1.41 in Dicho Aba-Garmama and 56.24±1.13 in Waligalte. ANSPCs were 1.85±0.08 in Ada-Buke Warego, 1.67±0.14 in Hagalo-Tulam, 1.54±0.14 in Dicho Aba-Garmama and 2.00±0.10 in Waligalte. The mean length of CI in months was 30.88±0.90 in Ada Buke Warego, 29.00±1.68 in Hagalo-Tulam, 28.62±1.62 in Dicho Aba-Garmama and 31.06±1.04 in Waligalte. The average year of longevity was 9.22±0.7 in Ada-Buke Warego, 9.08±0.8 in Hagalo-Tulam, 9.31±0.13 in Dicho Aba-Garmama and 9.21±0.8 in Waligalte, but there is no significant association (p≥0.05). AFC in months was 48.00±0.00 in local and 37.20±1.30 in crossbreeds. ANSPC was 1.92±0.06 in locals and 1.10±0.10 in hybrids. AFC for Horro and crossbred dairy cattle were 57.73±0.59 and 48.00±0.00months, respectively. The mean length of CI was 30.53±0.67 and 29.40±1.99months in natives and crossbreeds, respectively. The average year of longevity was 9.22±0.05 and 9.10±0.10years for local and hybrids, respectively. ANSPC for natural mating and AI were 1.92±0.05 and 1.10±0.10, respectively, with significant association (p<0.05). The reproductive performance of dairy cows in the study area was low, which required the improvement of husbandry practices.

Lessons to learn for better safeguarding of genetic resources during tree pandemics: The case of ash dieback in Europe

Ash dieback (ADB) has been threatening populations of European ash (Fraxinus excelsior & F. angustifolia) for more than three decades. Although much knowledge has been gathered in the recent past, practical conservation measures have been mostly implemented at local scale. Since range contraction in both ash species is likely to be exacerbated already in the near future by westward expansion of the emerald ash borer and climate change, systematic conservation frameworks need to be developed to avoid long-term population-genetic consequences and depletion of genomic diversity. In this article, we address the advantages and obstacles of conservation approaches aiming to conserve genetic diversity in situ or ex situ during tree pandemics. We are reviewing 47 studies which were published on ash dieback to unravel three important dimensions of ongoing conservation approaches or perceived conservation problems: i) conservation philosophy (i.e. natural selection, resistance breeding or genetic conservation), ii) the spatial scale (ecosystem, country, continent), and iii) the integration of genetic safety margins in conservation planning. Although nearly equal proportions of the reviewed studies mention breeding or active conservation as possible long-term solutions, only 17 % consider that additional threats exist which may further reduce genetic diversity in both ash species. We also identify and discuss several knowledge gaps and limitations which may have limited the initiation of conservation projects at national and international level so far. Finally, we demonstrate that there is not much time left for filling these gaps, because European-wide forest health monitoring data indicates a significant decline of ash populations in the last 5 years.