Traditional Fermented Sausage Research Articles

Whole-genome analysis suggesting probiotic potential and safety properties of Pediococcus pentosaceus DSPZPP1, a promising LAB strain isolated from traditional fermented sausages of the Basilicata region (Southern Italy).

Many lactic acid bacteria (LAB) strains are currently gaining attention in the food industry and various biological applications because of their harmless and functional properties. Given the growing consumer demand for safe food, further research into potential probiotic bacteria is beneficial. Therefore, we aimed to characterize Pediococcus pentosaceus DSPZPP1, a LAB strain isolated from traditional fermented sausages from the Basilicata region of Southern Italy. In this study, we analyzed the whole genome of the P. pentosaceus DSPZPP1 strain and performed in silico characterization to evaluate its applicability for probiotics and use in the food industry. The whole-genome assembly and functional annotations revealed many interesting characteristics of the DSPZPP1 strain. Sequencing raw reads were assembled into a draft genome of size 1,891,398 bp, with a G + C content of 37.3%. Functional annotation identified 1930 protein-encoding genes and 58 RNAs including tRNA, tmRNA, and 16S, 23S, and 5S rRNAs. The analysis shows the presence of genes that encode water-soluble B-group vitamins such as biotin, folate, coenzyme A, and riboflavin. Furthermore, the analysis revealed that the DSPZPP1 strain can synthesize class II bacteriocin, penocin A, adding importance to the food industry for bio-enriched food. The DSPZPP1 genome does not show the presence of plasmids, and no genes associated with antimicrobial resistance and virulence were found. In addition, two intact bacteriophages were identified. Importantly, the lowest probability value in pathogenicity analysis indicates that this strain is non-pathogenic to humans. 16 s rRNA-based phylogenetic analysis and comparative analysis based on ANI and Tetra reveal that the DSPZPP1 strain shares the closest evolutionary relationship with P. pentosaceus DSM 20336 and other Pediococcus strains. Analysis of carbohydrate active enzymes (CAZymes) identified glycosyl transferases (GT) as a main class of enzymes followed by glycoside hydrolases (GH). Our study shows several interesting characteristics of the isolated DSPZPP1 strain from fermented Italian sausages, suggesting its potential use as a promising probiotic candidate and making it more appropriate for selection as a future additive in biopreservation.

Genetic Identification and Technological Potential of Indigenous Lactic Acid Bacteria Isolated from Alheira, a Traditional Portuguese Sausage.

Alheira is a naturally fermented meat sausage traditionally made in the Portuguese region of Trás-os-Montes. Lactic acid bacteria (LAB) are the dominant microorganisms in alheira and can endow it with various technological properties. This study aimed (1) to characterize technological features and in vitro antimicrobial activity of LAB isolated from alheira, and (2) to reveal associations between such phenotypic characteristics and the isolates species identified through amplification and sequencing of the 16S ribosomal gene. Sixty-two LAB isolates were identified and Enterococcus (E.) faecium corresponded to 32.3% of isolates, followed by Leuconostoc (L.) mesenteroides (19.4%) and Latilactobacillus (Lb.) sakei (17.7%), aligning with previous research on traditional Portuguese fermented meat sausages. The phenotypic analysis of LAB isolates indicated diverse acidification capacities, proteolytic activities, and inhibitory effects against foodborne pathogens Listeria (L.) monocytogenes, Salmonella (S.) Typhimurium and Staphylococcus (S.) aureus. Overall, lactobacilli displayed high inhibition activity against the pathogens S. aureus, L. monocytogenes, and S. Typhimurium. Although the mechanisms for the inhibition of pathogen growth need to be further elucidated, these findings enhance our understanding of LAB diversity and functionality in alheira sausages, contributing to product safety and quality.

High-Pressure Processing Helps Meet the Escherichia coli O157:H7 and Shiga Toxin–Producing E. coli (STEC) Performance Standards for Beef Summer Sausage

The United States Department of Agriculture–Food Safety Inspection Service (USDA-FSIS) performance standards require that manufacturers of fermented beef sausages validate their processes to achieve a 5-log reduction of Escherichia coli O157:H7 and Shiga toxin–producing E. coli (STEC). Most processors rely on rapid fermentation to a low pH and a mild heat treatment to achieve the lethality performance standard. However, this process alters the sensorial characteristics of traditional fermented sausages. An alternative method to achieve lethality using high-pressure processing (HPP) during the manufacture of summer sausage with higher pH (5.0) and minimal heat treatment was evaluated. Sausages inoculated with circa 9.1 log CFU/g of E. coli O157:H7 and 8.9 log CFU/g of STEC were fermented to target pH values of 4.6 or 5.0. Subsequently, fermented sausages were heated to endpoint temperatures of 54.4°C, 48.9°C, or 43.3°C to the total process treatments of (1) Process A: pH 4.6 and 54.4°C, simulated cold air chilling, (2) Process B: pH 5.0 and 54.4°C, simulated cold air chilling, (3) Process C: pH 5.0 and 54.4°C, ice bath chilling, (4) Process D: pH 5.0 and 48.9°C, ice bath chilling, and (5) Process E: pH 5.0 and 43.3°C, ice bath chilling. After processing, the product was subjected to HPP (586 MPa; 4°C±2°C) for hold times of 1, 150, or 300 s and a nontreated (no HPP) control. All treatments subjected to HPP for 150 and 300 s reduced (P≤0.05) E. coli (O157:H7 and STEC) populations by>5.0 log CFU/g and >7.5 logCFU/g, respectively. The use of HPP allows for the production of more mild beef summer sausage (pH 5.0 and a mild thermal treatment of 43.3°C) while still achieving USDA-FSIS performance standards for lethality.

The Impact of HPP-Assisted Biocontrol Approach on the Bacterial Communities' Dynamics and Quality Parameters of a Fermented Meat Sausage Model.

Traditional foods are increasingly valued by consumers, whose attention and purchase willingness are highly influenced by other claims such as 'natural', 'sustainable', and 'clean label'. The purpose of the present study was to evaluate the impact of a novel non-thermal food processing method (i.e., HPP-assisted biocontrol combining mild high hydrostatic pressure, listeriophage Listex, and pediocin PA-1 producing Pediococcus acidilactici) on the succession of bacterial communities and quality of a fermented sausage model. A comparative analysis of instrumental color, texture, and lipid peroxidation revealed no significant differences (p > 0.05) in these quality parameters between non- and minimally processed fermented sausages throughout 60-day refrigerated storage (4 °C). The microbiota dynamics of biotreated and untreated fermented sausages were assessed by 16S rRNA next-generation sequencing, and the alpha and beta diversity analyses revealed no dissimilarity in the structure and composition of the bacterial communities over the analyzed period. The innovative multi-hurdle technology proposed herein holds valuable potential for the manufacture of traditional fermented sausages while preserving their unique intrinsic characteristics.

Effect of selected agents for ochratoxin A biocontrol on the colour, texture and volatile profile of dry-cured fermented sausages.

Traditional dry-cured fermented sausages favour the growth of an autochthonous microbial population, which plays an important role in their sensory aspects. However, some moulds can produce mycotoxins such as ochratoxin A (OTA). The biocontrol agents (BCAs) Debaryomyces hansenii FHSCC 253H and Staphylococcus xylosus FHSCC Sx8 have been demonstrated to reduce OTA production in dry-cured meat products, but their influence in the sensory characteristics of sausages has to be tested. The aim of this study was to evaluate the effect of these BCAs on the colour, texture and volatile profile of dry-cured fermented sausages. D. hansenii caused few differences in the tested parameters with respect to the control batch. S. xylosus modified the texture and colour, although the values found were within the range expected for dry-cured fermented sausages 'salchichón'. Additionally, the volatile profile revealed the potential antioxidant effect of both BCAs and their ability to produce compounds associated with the ripened aroma that could increase product acceptability. The results indicate that there were no inconveniences in implementing both BCAs during the processing of dry-cured fermented sausages 'salchichón'. Moreover, D. hansenii FHSCC 253H could improve the volatile profile of this product. © 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Effect of oat bran addition on the survival of selected probiotic strains in Turkish fermented sausage during cold storage

The aim of this study was to determine the probiotic survivability of selected probiotic in the oat-bran added sausages. In this study, traditional fermented Turkish sausage was produced by adding oat bran at levels of 0.0%, 1.0%, 2.5%, and 5.0% of raw meat, as well as inoculating it with probiotic bacteria strains Lactobacillus acidophilus DSM 20079, Lacticaseibacillus casei 431®, and Lactobacillus acidophilus NCFM. The effects of these additions on the sausage were investigated by creating 16 different groups of samples (4 groups for each level of oat bran and control group (no inoculation) + 3 groups inoculated with different probiotic strains). After fermentation, the inoculated sausages were stored under highly controlled conditions for 30 days. Sausage samples underwent physicochemical and microbiological analyses at different storage stages (days 0, 15, and 30). It was found that the survival of probiotic bacteria was at an acceptable level (8–9 log10 CFU/g). The addition of oat bran and the inoculation of probiotic bacteria increased the thiobarbituric acid (TBARS) number of the sausage. It has been found that a 5% oat bran content contributes to the survival of L. acidophilus DSM 20079 and reduces the number of yeasts and moulds. L. acidophilus DSM 20079 also had the longest life cycle when compared to the other probiotics. Despite probiotic bacteria's potential for increasing oxidation, certain study groups showed lower TBARS values. To further improve their functional properties, it may be beneficial to consider using different starter cultures or a combination of starter cultures.

Microbiological, Physicochemical and Sensorial Changes during the Ripening of Sucuk, a Traditional Turkish Dry-Fermented Sausage: Effects of Autochthonous Strains, Sheep Tail Fat and Ripening Rate

This study aimed to investigate the effects of autochthonous starter cultures (spontaneous fermentation, Lactiplantibacillus plantarum GM77, Staphylococcus xylosus GM92 or L. plantarum GM77 + S. xylosus GM92) isolated from sucuk (a traditional Turkish dry fermented sausage), the use of sheep tail fat (beef fat-control, sheep tail fat and beef fat + sheep tail fat) and the ripening rate (slow or fast) on the microbiological, physicochemical and sensorial changes during the ripening of sucuk. L. plantarum GM77 as a monoculture or mixed culture with S. xylosus GM92 exhibited good growth during fermentation and following days of ripening. S. xylosus GM92 remained at the inoculation level of 106 CFU/g. L. plantarum GM77 as a monoculture inhibited the growth of spontaneous Micrococcus/Staphylococcus in both the slow and fast ripening conditions. In the presence of L. plantarum GM77, the pH value decreased under 5.0 after the first three days of fermentation. The fast ripening yielded a lower mean aw and TBARS values compared to the slow ripening. Regarding TBARS value, the lowest mean value was determined in the presence of L. plantarum GM77 + S. xylosus GM92. The use of sheep tail fat caused an increase in TBARS; the highest mean value was determined in sucuk prepared with only sheep tail fat. The groups with L. plantarum GM77 yielded a higher mean L* value, while the highest a* value was determined in the group with L. plantarum GM77 + S. xylosus GM92. In addition, fast ripening caused an increase in the a* value. The L*, a* and b* values were not affected by the use of sheep tail fat. L. plantarum GM77 + S. xylosus GM92 groups demonstrated the best results in terms of general acceptability in both slow and fast ripening.

Identification of some yeast species in traditional Turkish fermented sausage with Vitek 2 compact system

Fermented sausage, produced by traditional methods, matures by fermentation of microbial flora originating from the raw materials and the place of production. The source of contamination of the meat industry with yeasts, which are widespread in the environment, are the surfaces of the tools and equipment used in processing. Although the presence of yeasts in meat products contributes to the formation of flavor and aroma, some yeast species can cause undesirable flavors, discoloration, and the formation of a soft texture. Therefore, our study aimed to determine the yeast profile of fermented sausages using the Vitek2 Compact System, in which various biochemical tests were performed. In the sausage samples was detected Candida zeylanoides in 56.25%, Candida sake in 52.10%, Pichia farinosa in 25%, Cryptococcus laurentii in 10.42%, Candida glabrata in 4.17%, and Rhodotorula glutinis in 10.42% yeast species. The difference in yeast species in fermented sausages varied depending on the microbial load of the raw material and compliance with hygiene regulations during processing and fermentation conditions.

Incidence of Polycyclic Aromatic Hydrocarbons in Portuguese Traditional Dry Smoked Meat Products Manufactured at Home for Self-Consumption and by Micro Industrial Plants

Polycyclic aromatic hydrocarbons (PAHs) concentration in dry meat products is a priority safety requirement, mainly in those processed under the traditional smoking procedure. In order to investigate the levels occurred in traditional dry fermented sausages manufactured by regional home producers, samples were collected in region of Central-East part of Portugal for comparison with those from one micro industrial plant situated in the same geographical area. In the former products, mean total PAHs level (1364.0 µg/kg) almost doubled that of industrial counterparts (750 µg/kg) with PAH8 representing 2.9 and 1.65% of those total contaminations, respectively. In both product groups, BaA and CHR were the most concentrated compounds within the PAH8 indicator (15.39 µg/kg and 13.66 µg/kg vs 5.67 µg/kg and 5.15 µg/kg). In home made products the BaP concentration surpassed the limit value of 5.0 µg/kg in 31.3% of the samples, with 3 of these cases more than doubling that amount. In addition, more than 93% of samples showed a BaP contamination higher than 1.0 µg/kg. By the contrary, among products processed under the industrial conditions that limit was never surpassed and 81.3% of the samples presented a BaP level lower than 1.0 µg/kg.

Microbiological effect of complete replacement of nitrites/nitrates with starter cultures in traditional raw-dried fermented sausage “Lukanka Panagyurska”

The article is focused on microbiological safety of the traditional raw-dried meat product “Lukanka Panagyurska”, produced by a starter cultures, containing Lactiplantibacillus plantarum GLP3 and Debaryomyces hansenii ATCC 36239 instead of using nitrates/nitrites. The aim of the study was to demonstrate that the starter cultures had a similar or better preservative effect as the traditional nitrates transformed into nitrites in the drying-ripening phase. The effect of the same starter cultures, produced by two different technological approaches on the survival of foodborne pathogens (Salmonella Typhimurium, Listeria monocytogenes, Escherichia coli and Clostridium perfringens) was examined. The study showed the presence of the lactic acid microorganisms at every stage of the production process of the raw-dried meat product. Zoonotic pathogens as S. Typhimurium and L. monocytogenes and sanitary indicator bacteria as E. coli and C. perfringens were combined to control the levels of pathogen inactivation. The preservative effect of the starter cultures resulting from lactic acid fermentation was more effective in comparison to that of nitrites/nitrates. The experiments proved that the microbiological safety of the raw-dried meat product was improved in comparison with the popular practice of adding nitrates/nitrites as a preservative.

Diversity and Safety Aspects of Coagulase-Negative Staphylococci in Ventricina del Vastese Italian Dry Fermented Sausage

Ventricina del Vastese is a traditional dry fermented sausage from Central Italy not yet characterized for the occurrence, identity and safety of coagulase-negative staphylococci (CNS), a bacterial group technologically important for this kind of product. Therefore, in this study, 98 CNS isolates from four manufacturers were differentiated using repetitive element palindromic PCR (Rep-PCR) and identified using 16S rRNA gene sequencing. These were examined for genes encoding biogenic amine (BA) production, resistance to aminoglycosides, β-lactams, tetracyclines and staphylococcal enterotoxins (SEs). Staphylococcus succinus (55%) predominated, followed by S. xylosus (30%), S. epidermidis (7.4%), S. equorum (3.1%), S. saprophyticus (3.1%) and S. warneri (1%). One S. succinus subsp. casei isolate was slightly β-hemolytic. SEs and the histidine decarboxylase gene hdcA were not detected, whereas the tyrosine decarboxylase gene tdcA was detected in four S. xylosus isolates. The blaZ beta-lactamase gene in an S. equorum isolate, tetracycline resistance genes tetK in six S. succinus isolates and tetA in one S. succinus isolate also bearing tetK were found. The product examined is characterized by a peculiar CNS species ratio and a low occurrence and diversity of AR transferable genes than found in other studies, as a probable consequence of production only with meat from animals raised in small farms with extensive rearing systems in which antibiotic usage is infrequent.

Effect of Hibicus sabdariffa on the Nutritional and Sensory Properties of Novel Naem Product

Naem is a traditional Asian fermented sausage made from the mixture of raw meat, cooked rice, garlic, salt, sugar, spices, and sodium nitrite. With consumers more concerned with healthy food choices, Hibiscus sabdariffa (HS) can be used as substitute for sodium nitrite in Naem preparation. Therefore, the objective of this study was to evaluate the effects of HS on the sensory, quality, and physicochemical analyses of Naem products. Four concentrations of HS were evaluated in this experiment: 1) 0%, 2) 1%, 3) 3% and 4) 5% HS. Treatments were analyzed for sensory evaluation by using a 9-point hedonic scale (trained panelists = 38). Physicochemical characteristics were evaluated for color (L*, a*, and b* values), pH, water activity, moisture (%), ash content, and lipid stability (TBARS). In addition, nutrition profiles, lactic acid bacteria, aerobic plate counts, Escherichia coli, and Listeria spp. were completed. SPSS with one-way ANOVA was used to evaluate any significant differences with p<0.05. The sensory evaluation revealed that Naem prepared with 3% HS had the highest overall acceptance scores (5.76), flavor (5.81), and taste (5.66). In addition, fermented Naem with 3% HS showed the highest scores of acceptability (92.2%), purchase intent (71.1%), and lactic acid bacteria counts (5.41 log CFU/g). The initial pH values, water activity, moisture (%), and ash content in this experiment ranged from 5.44-5.57, 0.93-0.94, 63.17-64.94%, and 1.51-1.74%, respectively. There was a significant (p<0.05) on color after 7 days storage at 3°C. Specifically, a* values were decreased in all treatments. The control treatment obtained the highest TBARS values (0.83 mg MDA/kg). No E. coli or Listeria spp. were detected. The results of this study indicate that Hibiscus sabdariffa can be used as a natural spice for Naem products which may help the meat industry increase market share through this innovative product.

Antioxidative effect of dry distilled rose petals extract in traditional Bulgarian dry fermented sausages with reduced nitrate content

The aim of study was to explore the dry distilled rose petals extract (DDRPE) addition to Bulgarian lukanka type dry fermented sausages with half-reduced nitrate content. There was studied the antioxidative effect of DDRPE in lukanka filling mass on color characteristics, pH, acid value (AV), peroxide value (POV), TBARS and sensory characteristics. Four samples: C100 - 0.500 g/kg nitrate without DDRPE, C50 - 0.250 g/kg nitrate without DDRPE, R1 - 0.250 g/kg nitrate and 1.140 g/kg DDRPE and R2 - 0.250 g/kg nitrate and 2.280 g/kg DDRPE were examined dynamically on the 1-st, the 10-th and 18-th d during the sausage processing. The color brightness (L*) at the end of the experiment was the highest in C100 and R1 while the redness (a*) and yellowness (b*) values were higher in R1 samples only. The pH was the lowest in R2 and R1. An increase in AV during the studied period was evidence for an outgoing lipolysis in all samples. In comparison to using 2.280 g/kg DDRPE (R2), the addition of 1.140 g/kg DDRPE (R1) demonstrates a better inhibitory effect on lipid peroxidation (POV, TBARS) of traditional Bulgarian dry fermented sausages. C100 had the highest sensory scores followed by R2.

Technological characterization and flavor-producing potential of lactic acid bacteria isolated from traditional dry fermented sausages in northeast China

Thirty-seven lactic acid bacteria (LAB) strains were isolated from traditional dry sausages collected from Northeast China, including Latilactobacillus sakei (29 strains), Lactiplantibacillus plantarum (4 strains), Latilactobacillus curvatus (2 strains), Weissella hellenica (1 strain), and Lactococcus lactis (1 strain). Some LAB strains had tolerance to high concentrations of sodium chloride (6%), sodium nitrite (150 mg/L NaNO2), and acid (pH 4.0). They showed good growth and acidification properties and antimicrobial activity. Among them, five LAB strains that exhibited the best technological properties were selected and inoculated in the sausage model to explore their roles in flavor development. The contents of total free amino acids (FAAs) decreased ranging from 109.11 mg/g to 58.06 mg/g. A total of 46 volatile compounds were identified and the contents of volatile compounds increased in the sausage model during fermentation. Partial least squares regression analysis showed that Lb. sakei HRB10, Lb. plantarum MDJ2, W. hellenica HRB6, and Lc. lactis HRB0 promoted the generation of FAAs and volatile compounds in the sausage model. These findings demonstrated that the autochthonous LAB species are promising for the production of sausage with better flavor and fermentation performance.

Reformulation of Traditional Fermented Tea Sausage Utilizing Novel (Digital) Methods of Analysis

The main objective of this paper was to investigate the effect of fat reduction on different quality traits of tea sausage. This study also aimed to deploy the following digital methods of analysis: three-dimensional (3D) laser imaging, computer vision system and oral processing. Three batches of tea sausage with different amounts of pork back fat were manufactured: control (25%), medium fat (17.5%) and low fat (10%). Samples for the analyses were taken on the production day and after 7, 14, 21, 28 and 35 days of ripening. The fat level significantly influenced shrinkage, texture, pH, aw, moisture and ash contents, peroxide value, acid number, number of chewing strokes, consumption time, eating rate and fat intake rate. Oxidative stability, colour and microbiological parameters were not affected by fat reduction. The results of the sensory analysis showed that the fat level can be reduced to 17.5% without negatively affecting the quality and sensory properties of the product. The ripening time of the fat-reduced tea sausage should be reduced to 28 days. A strong correlation between shrinkage and weight loss suggests the possibility of using 3D laser imaging in predicting weight loss and moisture content of dry sausages.

Technological properties and flavour formation potential of yeast strains isolated from traditional dry fermented sausages in Northeast China

The technological properties and flavour formation potential of yeast strains isolated from traditional dry fermented sausages were evaluated. A total of 46 yeast strains were isolated and characterized at the strain level by genotyping. Among them, 34 strains were selected for evaluation of technological properties (tolerance to acid, salt, and sodium nitrite stresses; proteolytic and lipolytic activities; and biogenic amine formation capacity) based on their genetic profiles. Then, seven strains (Debaryomyces hansenii HRB2, SH4, and HG2; Torulaspora delbrueckii DQ3 and HG7; Pichia kudriavzevii MDJ1; and Candida zeylanoides DQ7) were selected to investigate their flavour formation potential in a fermented sausage model. A total of 56 volatile compounds were detected in all models, of which 17 volatile compounds were the most important flavour contributors with an odour activity value (OAV) > 1. The most remarkable result was the significant production of acid, alcohol, and esters in the inoculated models, such as acetic acid, hexanoic acid, ethanol, phenethyl alcohol, ethyl acetate, and ethyl hexanoate. Of all the strains, D. hansenii SH4 was considered to be the best flavour producer because it produced multiple compounds with the highest OAVs, which are associated with the development of desirable sensory characteristics in dry sausages.

Metagenomic and metabolomic profiling reveals the correlation between the microbiota and flavor compounds and nutrients in fermented sausages

Understanding the interrelationships between the differentially abundant microorganisms and metabolites of traditional “Fuet” fermented sausages (FSs) and inoculated fermented sausages (IFSs) can help us identify key species that are missing from commercial starter cultures to reproduce the flavor compounds and nutrients of traditional Fuet FSs. IFSs, inoculated with P. pentosaceus, P. acidilactici, S. xylosus, S. carnosus (SBM-52) or P. pentosaceus, and S. xylosus (THM-17), were deficient in reproducing the volatilome profile (in particular esters, methyl aldehydes, and methyl ketones) of traditional Fuet FSs because of the lack of diverse Staphylococci (S. carnosus, S. xylosus, S. equorum, and S. saprophyticus). Moreover, the combination of Pediococcus and Staphylococcus were positively associated with amino acid, fatty acid, l-anserine, and l-carnosine levels. Pyridoxal and indolelactic acid levels were significantly increased in IFSs with the addition of P. acidilactici and S. carnosus, which were positively associated with vitamin B6 and tryptophan metabolic pathways.

Evaluation of the Antioxidant Effects of Postbiotics and Paraprobiotics in Lactic Acid Bacteria Isolated from Traditional Fermented Sausages

Recent studies in the field of probiotic biotechnology have shown that some non-living microorganisms or the metabolites they produce have at least as valuable biological activities as probiotic bacteria. Paraprobiotics are described as non-viable microbial cells that confer a benefit to the human or animal when administered in adequate amounts. Bioactive soluble metabolic byproducts produced by live probiotic microorganisms that impart some physiological benefit to the host are referred to as postbiotics. Live probiotic bacteria have been used for many years to strengthen the antioxidant defense system against oxidative stress that causes many diseases. The antioxidant activity of postbiotics and paraprobiotics in lactic acid bacteria isolated from twelve different hand-made fermented sausages was assessed using the DPPH radical scavenging assay in this research. It was determined that the free radical scavenging effect of postbiotics used in the study was between 5.65% and 76.04%, and the effect of paraprobiotics varied between 5.90% and 18.07%. According to the findings, antioxidant capacity of the postbiotics and paraprobiotics are strain-dependent and the postbiotics have higher antioxidant activity than the paraprobiotics.

Farklı balık türleri kullanarak fermente balık sucuklarının üretimi ve mikrobiyal kalitelerinin belirlenmesi

In this study frozen-thawed rainbow trout (Onchorhynchus mykiss) and seabass (Dicentrarchus labrax) fillets were used for preparing of dry fermented fish sausages. The total mesophilic aerob bacteria (TMAB), total psychrophilic aerob bacteria (TPAB), yeast–mould (YM), lactic acid bacteria (LB), Enterobactericeae (EB), Staphylococcus aureus (SA) changes of these dry fermented sausages were examined in the refrigerated storage at 6-8˚C. At the end of the storage period of 90 days, the results of the TMAB, TPAB, YM, LB, EB and SA counts of dry fermented seabass sausages were determined as 6.25, 7.01, 3.61, 5.31, <1.0 ve <1.0 log cfu/g, while, TMAB, TPAB, YM, LB, EB and SA counts of dry fermented trout sausages were found as 6.57, 7.20, 4.44, 5.14, <1.0 ve <1.0 log cfu/g, respectively. In this study both fermented fish sausages were determined as too much dried and exceeded the microbiological limit of TMAB at the end of the storage period of 90 days in the refrigerator. However, fermented seabass sausage reached the maximum level of YM count on the 10th day of storage, whereas fermented trout sausage reached this level on the 30th day of storage. Therefore, it is suggested that they should be packaged in vacuum packaging because of preventing too much drying and the growth of undesirable moulds. Additionally, the identification of microorganisms in fermented fish sausages would also be advised to determine desirable and undesirable microorganisms. Dry fermented fish sausage would be an alternative product to traditional dry fermented meat sausage in Turkey because of the health benefits of fish.

Competition between Starter Cultures and Wild Microbial Population in Sausage Fermentation: A Case Study Regarding a Typical Italian Salami (Ventricina).

The work reports a case study describing how the competition wild microflora vs. starter cultures affects the final product characteristics. This study regards an industrial lot of Ventricina, an Italian long-ripened traditional fermented sausages, produced using starter cultures. After ripening, some relevant organoleptic defects (off-odour, crust formation) were observed. Therefore, analyses were carried out in the inner and outer sausage section to explain this phenomenon. Microbiological analyses indicated a high meat batter contamination and metagenomic analyses evidenced the inability of LAB starter cultures to lead the fermentation process. The results of this not controlled fermentation were the accumulation of high levels of biogenic amines (including histamine) and the formation of a volatile profile different if compared with similar products. Indeed, the volatilome analysis revealed unusually high amounts of molecules such as isovaleric acid, propanoic acid, 1-propanol, which can be responsible for off-odours. This study demonstrated that starter culture use needs to be modulated in relation to production parameters to avoid safety and organoleptic concerns.