Toxoplasma Gondii IgM Antibodies Research Articles

Toxoplasmosis Examination With Anti-Toxo IgM And IgG Results In Petshop Staff

Toxoplasmosis is an infection caused by a single-celled parasite called Toxoplasma gondii. Infection is most commonly acquired from contact with cats and their feces or raw or undercooked meat. There are several risk factors that favor the occurrence of this infection including the presence of cats in the residence, cleaning of pet litter boxes, hand washing habits and consumption of raw or undercooked food. Petshop is a retail business that sells a variety of pets and also sells pet food, accessories, animal care and maintenance. The purpose of this study was to determine the exposure to toxoplasmosis with the results of Toxoplasma gondii IgG antibody examination and Toxoplasma gondii IgM antibody in petshop staff.This study is a descriptive study using cross sectional method with 25 respondents from petshop staff. The test method for toxoplasmosis detection used was Rapid (Immunochromatography). The data analysis technique used was univariate analysis to determine the percentage of seropositive results of Toxoplasma gondii IgG antibodies and Toxoplasma gondii IgM antibodies. Based on the results of the study obtained data 11 respondents seropositive anti-toxo IgG, 14 respondents did not have anti-toxo IgG and anti-toxo IgM and there were no respondents with positive anti-toxo IgM in Petshop officers. Therefore it can be concluded that 11 out of 25 respondents have been infected with Toxoplasma gondii.

Diagnostic significance of C-reactive protein and hematological parameters in acute toxoplasmosis.

Toxoplasmosis is a zoonosis caused by Toxoplasma gondii, which can be acquired by oral contact and may cause severe health problems especially for pregnant (congenital toxoplasmosis) and immunocompromised patients. This study aimed to verify the diagnostic significance of hematological parameters and C-reactive protein (CRP) for toxoplasmosis acute detection. A case-control study was carried out between December 2017 and May 2018, in samples of convenience independent of age and sex. The case group was formed by 25 patients with positive anti-Toxoplasma gondii IgG/IgM antibody and the control group was formed by 21 patients with non-positive anti-Toxoplasma gondii IgG/IgM antibody. The results of the hematological parameters and CRP were analyzed in these patients. The patients with Toxoplasma gondii IgM antibody reagent showed higher lymphocytes counting and lower neutrophils counting than the control group. C-reactive protein levels were not different between the groups case and control. ROC curve analysis highlighted that the cut-off value of > 32.00% for lymphocytes and < 57.50% for neutrophils were able to produce specificity higher than 90% for IgM antibody detection. The Naïve Bayes classifier was considered suitable (AUC ≈ 0.700) to separate both groups according to their white cell counting. Changes in lymphocytes and neutrophils may be useful parameters for toxoplasmosis identification and may be used as a tool in the complementary diagnosis of toxoplasmosis.Graphic abstract

Unpasteurised milk consumption as a potential risk factor for toxoplasmosis in females with recurrent pregnancy loss

In women with a bad obstetric history, certain infections are associated with recurrent foetal loss. One of the common infectious agents is a protozoan parasite, Toxoplasma gondii. The aim of this study was to assess unpasteurised milk consumption as a potential risk factor for toxoplasmosis in females with recurrent pregnancy loss from the province of Khyber Pakhtunkhwa, Pakistan. In this study, we recruited a total of 360 females, comprising a study group of 180 females with previous history of recurrent pregnancy loss and a control group of 180 females with no such history. Blood serum from the participants was analysed for Toxoplasma gondii IgM antibodies by enzyme linked immunosorbent assay. Among the study group, 23 (12.8%) females were serologically positive for IgM antibodies against Toxoplasma gondii, whilst 157 (87.2%) were IgM negative. In the control group, only two (4.8%) females were IgM positive, whilst 178 (95.2%) were IgM negative. Bad pregnancy outcome in the study group and control group was observed to be significantly different (p < .0001). In both of these groups, unpasteurised milk consumption was found as a major risk factor for Toxoplasma gondii infection. A routine serological investigation should be carried out in pregnant women to rule out toxoplasmosis and reduce the risk of recurrent pregnancy loss as well as congenital toxoplasmosis in newborns.Impact statementWhat is already known on this subject? Seropositivity for Toxoplasma gondii antibodies ranges from 7% to 51% in different regions of the world. The prevalence rate varies because of differences in climate, culture, food habits, behaviour, personal hygiene and cooking habits of different societies and ethnic groups. Various risk factors have been identified that contribute to a high prevalence rate of the disease, including consumption of raw or poorly cooked meat, physical contact with cats or cat litter, consumption of unwashed raw vegetables and fruits, drinking of contaminated water and milk. We presumed that consuming unpasteurised milk could be a potential risk factor for developing toxoplasmosis in pregnant women.What the results of this study add? This study demonstrates high seroprevalence of Toxoplasma gondii antibodies in females of child bearing age that have consumed unpasteurised milk and is a potential risk factor for developing toxoplasmosis.What the implications are of these findings for clinical practice and/or further research? Our findings suggest that primary preventive measures (personal hygiene, frequent hand washing and consuming pasteurised milk) should be taken by health surveillance authorities to focus on families, especially pregnant women, to educate them about personal hygiene, contact with cattle or using their milk and milk products. The latter is especially important to aware them about the hazards of consuming unpasteurised and contaminated milk.

Analysis of Toxoplasma Gondii Infection Rate in Patients with Hematological Diseases

To investigate the infection rate of Toxoplasma gondii in patients with hematological diseases. The Toxoplasma gondii IgM antibody in 200 patients with hematological diseases were tested, at the same time, IgM antibody in the persons received physical examination and other patients with common clinical diseases also were test, and their detection results were compared. The positive rate of Toxoplasma gondii IgM antibody in patients with hematological diseases was 7.50%, the positive rate in persons received physical examination was 0.67%, and the positive rate in patients with other common clinical diseases was 1.20%. The positive rate of IgM antibody in patients with hematological diseases was statistically significantly higher than that in the latter two kinds of persons(P<0.05). Among the patients with hematological diseases, the positive rate of Toxoplasma gondii IgM antibody in patients with bone marrow neoplastic diseases was 10.32%, which was statistically significantly higher than that in patients with bone marrow non-neoplastic diseases (2.70%). Patients with hematological diseases are susceptible to Toxoplasma gondii, and to whom enough attention should be paid.

Analysis of Toxoplasma Gondii Infection Rate in Patients with Hematological Diseases

To investigate the infection rate of Toxoplasma gondii in patients with hematological diseases. The Toxoplasma gondii IgM antibody in 200 patients with hematological diseases were tested, at the same time, IgM antibody in the persons received physical examination and other patients with common clinical diseases also were test, and their detection results were compared. The positive rate of Toxoplasma gondii IgM antibody in patients with hematological diseases was 7.50%, the positive rate in persons received physical examination was 0.67%, and the positive rate in patients with other common clinical diseases was 1.20%. The positive rate of IgM antibody in patients with hematological diseases was statistically significantly higher than that in the latter two kinds of persons(P<0.05). Among the patients with hematological diseases, the positive rate of Toxoplasma gondii IgM antibody in patients with bone marrow neoplastic diseases was 10.32%, which was statistically significantly higher than that in patients with bone marrow non-neoplastic diseases (2.70%). Patients with hematological diseases are susceptible to Toxoplasma gondii, and to whom enough attention should be paid.

Toxoplasmoz şüpheli hastalarda Toxoplasma gondii seropozitifliğinin kemilüminesan mikropartikül immunolojik test (CMIA) yöntemi ile araştırılması

Aim. Toxoplasmosis, is a parasitic disease can be seen as an acquired or congenital caused by Toxoplasma gondii. In the study, we aimed to determine seroprevalence of the antibodies against T gondii in the patients admitted to Sivas Numune Hospital in a period of one year. Method. Antibody results for Toxoplasma gondii which were assayed with chemiluminescent microparticle immunological test (CMIA) (Abbott-Architect system, Weisbaden, Germany) from the serum samples of the patients with 0-80 years old who applied to Sivas Numune Hospital’s clinics between January 2012-January 2013 were analysed retrospectively. In the study, anti-toxoplasma IgG and IgM antibodies were investigated in sera of 809 patients. Results. The rates of Toxoplasma gondii IgM antibodies were found to be 0.7% (5/772) and that of Toxoplasma gondii IgG antibodies 35.3% (86/244). In only one patient either IgG or IgM antibodies were obtained positive (0.6%-1/207). Conclusion. As a result, seroprevalence of toksoplasmosis should be monitored and especially for individuals who are seronegative should take precautions.

An investigation into the seroprevalence ofToxoplasma gondii,Bartonellaspp., feline immunodeficiency virus (FIV), and feline leukaemia virus (FeLV) in cats in Addis Ababa, Ethiopia

Toxoplasma gondii and Bartonella spp. are zoonotic pathogens of cats. Feline immunodeficiency virus (FIV), and feline leukaemia virus (FeLV) are immunosuppressive viruses of cats that can affect T. gondii oocyst shedding. In this study, the prevalence of antibodies to T. gondii, Bartonella spp., FIV, as well as FeLV antigens were determined in sera from feral cats (Felis catus) from Addis Ababa, Ethiopia. Using the modified agglutination test, IgG antibodies to T. gondii were found in 41 (85.4%) of the 48 cats with titres of 1:25 in one, 1:50 in one, 1:200 in six, 1:400 in six, 1:800 in six, 1:1600 in eight, and 1:3200 in 13 cats. Toxoplasma gondii IgM antibodies were found in 11/46 cats tested by ELISA, suggesting recent infection. Antibodies to Bartonella spp. were found in five (11%) of 46 cats tested. Antibodies to FIV or FeLV antigen were not detected in any of the 41 cats tested. The results indicate a high prevalence of T. gondii and a low prevalence of Bartonella spp. infection in cats in Ethiopia.

The national neonatal screening programme for congenital toxoplasmosis in Denmark: results from the initial four years, 1999–2002

Aims: To describe the outcome of four years’ nationwide neonatal screening for congenital toxoplasmosis in liveborn newborns. Methods: Congenital toxoplasmosis was diagnosed if specific Toxoplasma gondii IgM antibodies were detected...