Toxigenic Cyanobacteria Research Articles

Toxigenic Cyanobacteria and Microcystins in a Large Northern Oligotrophic Lake Onego, Russia

Toxigenic cyanobacteria and microcystins in the oligotrophic pelagic zone and mesotrophic bay of Lake Onego—the second largest lake in Europe—were found for the first time. Microscopic analysis revealed that Dolichospermum lemmermannii, D. circinale and D. spiroides dominated in bloom spots in the oligotrophic zone of the lake and D. flos-aquae and Microcystis aeruginosa OKin the eutrophic bay. The abundance of cyanobacteria in bloom spots is potentially hazardous for humans and animals. PCR-analysis showed that mcyA gene involved in microcystin biosynthesis was found in cyanobacteria of the genera Dolichospermum and Microcystis. Five structural variants of intracellular microcystins were detected in a trace amount using high-performance liquid chromatography–mass-spectrometry of high resolution. The most hazardous hepatotoxin, MC-LR, was found only in the eutrophic bay. In the present study, the reasons for the low cyanotoxin content in the phytoplankton dominated by Dolichospermum are discussed. The findings of our study make a significant contribution to the accumulation of facts which state that toxigenic cyanobacterial blooms can occur in large oligotrophic lakes.

The prevalence and persistence of microcystin in seven subtropical reservoirs in China and associated potential risks for human health

Microcystins (MCs) are the most frequent cyanotoxins occurring in inland freshwaters, posing a serious threat to drinking water safety and human health. However, the distribution and risk of MCs in subtropical reservoirs in China are still unclear. In this study, we investigated the MCs pollution in seven subtropical reservoirs in Fujian Province, China. The results showed that most samples (87.74%) from all reservoirs were in a mesotrophic state during the survey period, and a variety of potential toxigenic cyanobacteria were detected, which resulted in the detection rate of MCs reaching 100%. The MCs concentration of surface samples ranged from 0.19 to 1.06 μg/L, and the proportion of MC-YR (43.72%) was the highest, followed by MC-RR (37.88%). The maximum was usually obtained in warmer seasons. The MCs concentration in stratified water samples ranged from 0.20 to 0.54 μg/L, while the MCs concentration in bottom water samples was sometimes higher than that in surface samples. MCs were positively correlated with most of the physicochemical parameters and the densities of phytoplankton phyla, but only negatively correlated with water depth, total nitrogen and nitrate nitrogen. MCs concentration was significantly positively correlated with the density of Microcystis and Cylindrospermopsis. Finally, risk assessments of MCs indicate that the risk from MCs pollution in all reservoirs studied remains low, particularly to humans. However, since the reservoir has different community compositions of toxin-producing cyanobacteria from that of shallow lakes, the long-term and regular monitoring and study of cyanotoxins should be strengthened in future under a changing climate.

Impact of climate change on waterborne infections and intoxications.

Progressive climate change holds the potential for increasing human health risks from waterborne infections and intoxications, e. g. through an increase in pathogen concentrations in water bodies, through the establishment of new pathogens or through possible changes in pathogen properties. This paper presents some examples of potential impacts of climate change in Germany. Non-cholera Vibrio occur naturally in seawater, but can proliferate significantly in shallow water at elevated temperatures. In the case of Legionella, climate change could lead to temporary or longer-term increased incidences of legionellosis due to the combination of warm and wet weather. Higher temperatures in piped cold water or lower temperatures in piped hot water may also create conditions conducive to higher Legionella concentrations. In nutrient-rich water bodies, increased concentrations of toxigenic cyanobacteria may occur as temperatures rise. Heavy rainfall following storms or prolonged periods of heat and drought can lead to increased levels of human pathogenic viruses being washed into water bodies. Rising temperatures also pose a potential threat to human health through pathogens causing mycoses and facultatively pathogenic micro-organisms: increased infection rates with non-tuberculous mycobacteria or fungi have been documented after extreme weather events.

Characterization of Potential Threats from Cyanobacterial Toxins in Lake Victoria Embayments and during Water Treatment.

Africa’s water needs are often supported by eutrophic water bodies dominated by cyanobacteria posing health threats to riparian populations from cyanotoxins, and Lake Victoria is no exception. In two embayments of the lake (Murchison Bay and Napoleon Gulf), cyanobacterial surveys were conducted to characterize the dynamics of cyanotoxins in lake water and water treatment plants. Forty-six cyanobacterial taxa were recorded, and out of these, fourteen were considered potentially toxigenic (i.e., from the genera Dolichospermum, Microcystis, Oscillatoria, Pseudanabaena and Raphidiopsis). A higher concentration (ranging from 5 to 10 µg MC-LR equiv. L−1) of microcystins (MC) was detected in Murchison Bay compared to Napoleon Gulf, with a declining gradient from the inshore (max. 15 µg MC-LR equiv. L−1) to the open lake. In Murchison Bay, an increase in Microcystis sp. biovolume and MC was observed over the last two decades. Despite high cell densities of toxigenic Microcystis and high MC concentrations, the water treatment plant in Murchison Bay efficiently removed the cyanobacterial biomass, intracellular and dissolved MC to below the lifetime guideline value for exposure via drinking water (<1.0 µg MC-LR equiv. L−1). Thus, the potential health threats stem from the consumption of untreated water and recreational activities along the shores of the lake embayments. MC concentrations were predicted from Microcystis cell numbers regulated by environmental factors, such as solar radiation, wind speed in the N–S direction and turbidity. Thus, an early warning through microscopical counting of Microcystis cell numbers is proposed to better manage health risks from toxigenic cyanobacteria in Lake Victoria.

Micrometer scale polystyrene plastics of varying concentrations and particle sizes inhibit growth and upregulate microcystin-related gene expression in Microcystis aeruginosa

Microplastics (MPs) are a concerning environmental pollutant due to their adverse effects on aquatic organisms. However, the dose- and size-dependent effects of MPs on toxigenic cyanobacteria have not been extensively studied. Herein, we explored the effects of polystyrene MPs (PS-MPs) of varying particle sizes and concentrations on the growth and physiology of Microcystis aeruginosa. The results showed that exposure to 1 µm PS-MPs at a concentration of 2–10 mg L–1 significantly inhibited the growth of M. aeruginosa in a concentration-dependent manner. After 12 days of exposure, high concentrations of 1 µm PS-MPs (≥ 2 mg L–1) increased levels of reactive oxygen species. Following exposure to 5 mg L–1 PS-MPs of different particle sizes, algal growth was inhibited and oxidative stress was induced by 0.5 and 1 µm PS-MPs. At the molecular level, transcription of the atpB gene was generally downregulated in all PS-MPs treatments, while ftsH and fabZ were upregulated. Exposure to PS-MPs also altered the transcription levels of microcystin-related genes (mcyA and mcyH), causing more microcystin to be produced by M. aeruginosa. The results will be useful for understanding the toxicity of MPs toward toxigenic cyanobacteria, and evaluating the ecological risks of MPs in aquatic environments.

High nutrient loading and climatic parameters influence the dominance and dissimilarity of toxigenic cyanobacteria in northern bays of Lake Victoria

Eutrophication of Lake Victoria led to changes in its phytoplankton communities. However, different levels of eutrophication exist in the open lake and the bays, and between embayments. This study utilized spatial and temporal sampling of Napoleon Gulf and Murchison Bay, exhibiting different trophic conditions. Over one year, we investigated phytoplankton biomass, richness, diversity and dissimilarity, and related the dynamics of the dominant species to the limnological and climatic conditions. The results confirmed that Napoleon Gulf and Murchison Bay showed large differences in eutrophication status, with lower nutrient concentrations in Napoleon Gulf than in Murchison Bay, where a strong gradient was observed from inshore to offshore areas. These nutrient dynamics resulted in a 4 to 10 fold higher chlorophyll-a in Murchison Bay than in Napoleon Gulf. From the embayments, 135 phytoplankton taxa were recorded with no significant differences in alpha diversity. However, high dissimilarity in community structure was observed in beta diversity, mostly due to a turnover among the dominant toxigenic species. Thus, from a similar species pool, there was a shift in the dominant toxigenic cyanobacteria from Microcystis flos-aquae and M. aeruginosa in Murchison Bay, Dolichospermum circinale and Planktolyngbya circumcreta in Napoleon Gulf to D. circinale in the offshore stations. These cyanobacteria are toxigenic taxa with known health hazards. Using partial least square models, we showed that both climatic variables (e.g. wind, solar radiation) and levels of inorganic dissolved nutrients (e.g. SRP, NO3–, and NH4+) are the main drivers of differences and dominance in cyanobacteria communities in northern Lake Victoria.

The Effects of Low-Intensity Ultrasound on Toxigenic Cyanobacteria

The Effects of Low-Intensity Ultrasound on Toxigenic Cyanobacteria

Molecular and morphological characterization of a novel dihydroanatoxin-a producing Microcoleus species (cyanobacteria) from the Russian River, California, USA

Reports of anatoxins poisoning of wildlife and domestic animals by toxigenic cyanobacteria in streams and rivers are increasing globally. Little is known about the taxonomy, morphology and genomics of anatoxins producing species, limiting our knowledge about their environmental preferences. We isolated three benthic non-heterocystous filamentous cyanobacterial strains from the Russian River in Northern California (USA), which produce anatoxin-a and dihydroanatoxin-a. Both 16S rRNA and protein sequence phylogenetic analyses showed that the strains represent a distinct new member of the cyanobacterial genus Microcoleus (Oscillatoriales). A novel species, Microcoleus anatoxicus is described and accompanied with light microscope photomicrographs, toxin profiles and the complete anatoxin-a gene cassette with the first description of the anaK gene in Microcoleus.

Responses to local and global stressors in the large southern perialpine lakes: Present status and challenges for research and management

Here, we review the state-of-the-art of limnological investigations in the large and deep lakes south of the Alps (DSLs), lakes Garda, Maggiore, Como, Iseo and Lugano. In the pre-industrial age, these lakes were ultra- or oligotrophic. Increasing anthropogenic pressure and the impact of global warming has led to an acceleration of eutrophication and a decrease in the frequency of full mixing episodes, which have induced a state of meromixis in lakes Lugano and Iseo. In the last two decades, other changes have been identified, including fundamental variations in the long-term dynamics and structure of phytoplankton communities, identification of new toxigenic cyanobacteria and cyanotoxins, increases in the introduction and establishment of allochthonous species, and continuous detection of new, emerging chemical pollutants. Overall, these fundamental changes are quickly transforming the features of the DSLs. The ability to document and reconstruct changes in lake aquatic biota and micropollutants was strongly dependent on both research efforts and the availability of technologies to appraise these changes. In this context, the ongoing adoption of new technological tools, such as high-frequency monitoring and high throughput sequencing, is opening the way to a new level of comprehension of physical, chemical and biological processes, and aquatic biodiversity. New outlooks and new conceptual frameworks are necessary to cope with the huge dimensions of high-throughput data and analysis of big data.

Glyphosate's Synergistic Toxicity in Combination with Other Factors as a Cause of Chronic Kidney Disease of Unknown Origin.

Chronic kidney disease of unknown etiology (CKDu) is a global epidemic. Sri Lanka has experienced a doubling of the disease every 4 or 5 years since it was first identified in the North Central province in the mid-1990s. The disease primarily affects people in agricultural regions who are missing the commonly known risk factors for CKD. Sri Lanka is not alone: health workers have reported prevalence of CKDu in Mexico, Nicaragua, El Salvador, and the state of Andhra Pradesh in India. A global search for the cause of CKDu has not identified a single factor, but rather many factors that may contribute to the etiology of the disease. Some of these factors include heat stroke leading to dehydration, toxic metals such as cadmium and arsenic, fluoride, low selenium, toxigenic cyanobacteria, nutritionally deficient diet and mycotoxins from mold exposure. Furthermore, exposure to agrichemicals, particularly glyphosate and paraquat, are likely compounding factors, and may be the primary factors. Here, we argue that glyphosate in particular is working synergistically with most of the other factors to increase toxic effects. We propose, further, that glyphosate causes insidious harm through its action as an amino acid analogue of glycine, and that this interferes with natural protective mechanisms against other exposures. Glyphosate’s synergistic health effects in combination with exposure to other pollutants, in particular paraquat, and physical labor in the ubiquitous high temperatures of lowland tropical regions, could result in renal damage consistent with CKDu in Sri Lanka.

A Novel Multiplex PCR-based Technique for Detection of Toxigenic Cyanobacteria

A Novel Multiplex PCR-based Technique for Detection of Toxigenic Cyanobacteria

Mesohaline conditions represent the threshold for oxidative stress, cell death and toxin release in the cyanobacterium Microcystis aeruginosa

As aquatic ecosystems become increasingly affected by hydrologic alterations, drought and sea level rise a need exists to better understand the biological effects of elevated salinity on toxigenic cyanobacteria such as Microcystis aeruginosa. This study investigated the impacts of oligohaline/low mesohaline conditions and exposure time on selected physiological and biochemical responses in M. aeruginosa including cell viability, oxidative stress, antioxidant responses, in addition to microcystin synthesis and release into the surrounding environment. M. aeruginosa was able to grow in most test salinity treatments (1.4–10 ppt), as supported by cell abundance data and chlorophyll-a (chl-a) concentrations. Physiological data showed that after certain salinity thresholds (∼7ppt) were surpassed, salt stress had cascading effects, such as increased ROS production and lipid peroxidation, potentiating the decline in cellular viability. Furthermore, elevated salinity induced oxidative stress which was concomitant with a decrease in cell abundance, chl-a concentration and photochemical efficiency in the 7–10 ppt treatments. M. aeruginosa did not synthesize microcystins (MCs) in response to increased saline conditions, and mcy-D expression was not correlated with either salinity treatment or extracellular MC concentrations, indicating that salinity stress could inhibit toxin production and that released toxins were likely synthesized prior to exposure. Additionally, extracellular MC concentrations were not correlated with decreased cellular integrity, as evidenced by SYTOX analyses, suggesting that toxins may be released through mechanisms other than cellular lysis. Results from this study support that M. aeruginosa can survive with limited negative impacts to cellular structure and function up to a certain threshold between 7–10 ppt. However, after these thresholds are surpassed, there is radical decline in cell health and viability leading to toxin release. This work underscores the importance of understanding the balance between ROS production and antioxidant capacities when assessing the fate of M. aeruginosa under mesohaline conditions.

Distribution of &lt;em&gt;Cylindrospermopsis raciborskii&lt;/em&gt; (Cyanobacteria) in Sri Lanka

Sri Lanka is a tropical continental island which consists of 103 natural rivers and over ten thousand man-made lakes. Majority of these water resources are known to be contaminated with different types of toxigenic cyanobacteria making water unsuitable for human and animal consumption. Cylindrospermopsis raciborskii, a toxin producing tropical cyanobacterium, recently recorded at high abundances in lentic waters of the country. This species is highly adaptive and exhibit different morphotypes: straight, coiled and sigmoid- shaped trichomes under different environmental conditions. Distribution and abundance of the species is mainly depending on the environmental factors and therefore remedies can be proposed to keep the populations under control. This preliminary study was conducted to understand the prevalence of C. raciborskii, in Sri Lankan reservoirs. Sixty three reservoirs representing all climatic regions with varying environmental conditions were assessed using standard procedures. The results of the study reviewed that C. raciborskii is a dominant cyanobacterium species especially during the dry season in shallow urban reservoirs, terminal reservoirs in large cascades, reservoirs with low flushing rates and deep reservoirs with persistent water stratification. Morphological variations were common in urban and shallow reservoirs that maintain high water retention time. They commonly occur in coiled–shape. However, in eutrophic water bodies they are appeared to support other morphotypes: straight, sigmoid or tricomatous. Further, it was noticed that the cell size of C. raciborskii is exceptionally large (width 3.5 µm and length 7µm) in a high altitudinal wet–zone reservoir that usually maintains temperature range of 10oC - 20oC throughout the year. The results showed that C. raciborskii is widely distributed all over the country and the presence of several morphotypes signifies its potential for rapid distribution.

Reconstructing a long-term record of microcystins from the analysis of lake sediments.

Based on an analysis of sediment cores from Baptiste Lake (Alberta, Canada), we quantified century-scale trends in cyanobacteria and cyanotoxins, and identified possible drivers of toxigenic cyanobacteria. We measured concentrations of microcystins and pigments preserved in the sediment as proxies of toxigenic cyanobacteria and phytoplankton communities, respectively, while fossil diatom assemblages were used to infer past nutrient concentrations. Microcystins were detected in older sediments (ca. 1800s), pre-dating any significant alteration to the watershed. This demonstrates that toxigenic cyanobacteria may not be a recent phenomenon in eutrophic ecosystems. The dominant variants of microcystin throughout the sediment core were microcystin-LA and microcystin-LR. Other congeners including -LY, -7dmLR, -WR, -LF, -YR, and -LW (-RR was not detected) were mainly found in the upper layers of sediment (post 1980s). Starting in the 1990s, concentrations of microcystins both in the water column and in the sediment record increased in parallel. Total sediment microcystins were strongly correlated with historical nitrogen and phosphorus concentrations inferred from diatom assemblages (r=0.80-0.81, p<0.001, n=22); both nutrients increased over the past two decades coincident with the intensification of agriculture. Microcystins also tracked the rise in cyanobacterial pigments present throughout the core. In contrast, we found no relationship between climate-related variables and sediment microcystin concentrations, although such relationships were detected over the monitoring record with respect to water column concentrations. Overall, the rise in sediment microcystins was much greater than the rise in sediment cyanobacteria and diatom inferred nutrient concentrations. Furthermore, we demonstrate that the reconstruction of the microcystin sediment record can provide important insight for the development of realistic lake management goals. Applying this analytical approach to different lakes and regions of the world, where both natural and anthropogenic gradients vary, has the potential to markedly improve our understanding of long-term drivers of cyanotoxin production.

Presence of the Cyanotoxin Microcystin in Arctic Lakes of Southwestern Greenland.

Cyanobacteria and their toxins have received significant attention in eutrophic temperate and tropical systems where conspicuous blooms of certain planktonic taxa release toxins into fresh water, threatening its potability and safe use for recreation. Although toxigenic cyanobacteria are not confined to high nutrient environments, bloom-forming species, or planktonic taxa, these other situations are studied les often studied. For example, toxin production in picoplankton and benthic cyanobacteria—the predominant photoautotrophs found in polar lakes—is poorly understood. We quantified the occurrence of microcystin (MC, a hepatotoxic cyanotoxin) across 18 Arctic lakes in southwestern Greenland. All of the focal lakes contained detectable levels of MC, with concentrations ranging from 5 ng·L−1 to >400 ng·L−1 during summer, 2013–2015. These concentrations are orders of magnitude lower than many eutrophic systems, yet the median lake MC concentration in Greenland (57 ng·L−1) was still 6.5 times higher than the median summer MC toxicity observed across 50 New Hampshire lakes between 1998 and 2008 (8.7 ng·L−1). The presence of cyanotoxins in these Greenlandic lakes demonstrates that high latitude lakes can support toxigenic cyanobacteria, and suggests that we may be underestimating the potential for these systems to develop high levels of cyanotoxins in the future.

Elucidation of Taste- and Odor-Producing Bacteria and Toxigenic Cyanobacteria in a Midwestern Drinking Water Supply Reservoir by Shotgun Metagenomic Analysis.

While commonplace in clinical settings, DNA-based assays for identification or enumeration of drinking water pathogens and other biological contaminants remain widely unadopted by the monitoring community. In this study, shotgun metagenomics was used to identify taste-and-odor producers and toxin-producing cyanobacteria over a 2-year period in a drinking water reservoir. The sequencing data implicated several cyanobacteria, including Anabaena spp., Microcystis spp., and an unresolved member of the order Oscillatoriales as the likely principal producers of geosmin, microcystin, and 2-methylisoborneol (MIB), respectively. To further demonstrate this, quantitative PCR (qPCR) assays targeting geosmin-producing Anabaena and microcystin-producing Microcystis were utilized, and these data were fitted using generalized linear models and compared with routine monitoring data, including microscopic cell counts, sonde-based physicochemical analyses, and assays of all inorganic and organic nitrogen and phosphorus forms and fractions. The qPCR assays explained the greatest variation in observed geosmin (adjusted R(2) = 0.71) and microcystin (adjusted R(2) = 0.84) concentrations over the study period, highlighting their potential for routine monitoring applications. The origin of the monoterpene cyclase required for MIB biosynthesis was putatively linked to a periphytic cyanobacterial mat attached to the concrete drinking water inflow structure. We conclude that shotgun metagenomics can be used to identify microbial agents involved in water quality deterioration and to guide PCR assay selection or design for routine monitoring purposes. Finally, we offer estimates of microbial diversity and metagenomic coverage of our data sets for reference to others wishing to apply shotgun metagenomics to other lacustrine systems. Cyanobacterial toxins and microbial taste-and-odor compounds are a growing concern for drinking water utilities reliant upon surface water resources. Specific identification of the microorganism(s) responsible for water quality degradation is often complicated by the presence of co-occurring taxa capable of producing these undesirable metabolites. Here we present a framework for how shotgun metagenomics can be used to definitively identify problematic microorganisms and how these data can guide the development of rapid genetic assays for routine monitoring purposes.

ACQUA: Automated Cyanobacterial Quantification Algorithm for toxic filamentous genera using spline curves, pattern recognition and machine learning

Toxigenic cyanobacteria are one of the main health risks associated with water resources worldwide, as their toxins can affect humans and fauna exposed via drinking water, aquaculture and recreation. Microscopy monitoring of cyanobacteria in water bodies and massive growth systems is a routine operation for cell abundance and growth estimation. Here we present ACQUA (Automated Cyanobacterial Quantification Algorithm), a new fully automated image analysis method designed for filamentous genera in Bright field microscopy. A pre-processing algorithm has been developed to highlight filaments of interest from background signals due to other phytoplankton and dust. A spline-fitting algorithm has been designed to recombine interrupted and crossing filaments in order to perform accurate morphometric analysis and to extract the surface pattern information of highlighted objects. In addition, 17 specific pattern indicators have been developed and used as input data for a machine-learning algorithm dedicated to the recognition between five widespread toxic or potentially toxic filamentous genera in freshwater: Aphanizomenon, Cylindrospermopsis, Dolichospermum, Limnothrix and Planktothrix. The method was validated using freshwater samples from three Italian volcanic lakes comparing automated vs. manual results. ACQUA proved to be a fast and accurate tool to rapidly assess freshwater quality and to characterize cyanobacterial assemblages in aquatic environments.

Persistent and delayed effects of toxic cyanobacteria exposure on life history traits of a common zooplankter

Anthropogenic eutrophication has resulted in shifts in phytoplankton community composition worldwide which represent dramatic changes in resource quality and availability for grazers such as rotifers. For these grazers, harmful algal blooms may have consequences that persist across several generations. We hypothesized that rotifers exposed to a pulse of the toxigenic cyanobacterium Microcystis aeruginosa, would suffer demographic and physiological effects that decreased their ability to recover after cyanobacteria exposure. Additionally, we hypothesized that rotifer population recovery after harmful algal blooms is modulated by delayed effects of pre-bloom food availability. We used laboratory experiments to test the effects of switching from a high quality diet to toxigenic cyanobacteria on the physiological condition and associated life history changes of the common rotifer Brachionus calyciflorus. We found that M. aeruginosa exposure decreased fecundity of rotifers by 51.5%, and early exposure to high levels of the high-quality food Chlamydomonas sp. did not ameliorate this negative effect. Rotifers exposed to Microcystis produced lower quality offspring (by 16.6%). However, we found that the effect of Microcystis on offspring body size was dependent on the density of food available in early life. Exposure to high-density food for the first 3 d of life tempered the negative effects of Microcystis exposure, whereas initial exposure to low-density food resulted in a 9.0% decrease in offspring length. We found that the negative effects of exposure to toxigenic cyanobacteria may accumulate across generations and limit the ability of rotifer populations to withstand the predicted increasing frequency and duration of harmful algal blooms.

Cyanophages Infection of Microcystis Bloom in Lowland Dam Reservoir of Sulejów, Poland.

An increased incidence of cyanobacterial blooms, which are largely composed of toxigenic cyanobacteria from the Microcystis genus, leads to a disruption of aquatic ecosystems worldwide. Therefore, a better understanding of the impact of environmental parameters on the development and collapse of blooms is important. The objectives of the present study were as follows: (1) to investigate the presence and identity of Microcystis-specific cyanophages capable of cyanobacterial cell lysis in a lowland dam reservoir in Central Europe; (2) to investigate Microcystis sensitivity to phage infections with regard to toxic genotypes; and (3) to identify key abiotic parameters influencing phage infections during the summer seasons between 2009 and 2013. Sequencing analysis of selected g91 gene amplification products confirmed that the identified cyanophages belonged to the family Myoviridae (95 % homology). Cyanophages and Microcystis hosts, including toxic genotypes, were positively correlated in 4 of the 5 years analyzed (r = 0.67-0.82). The average percentage of infected Microcystis cells varied between 0.1 and 32 %, and no particular sensitivity of the phages to toxigenic genotypes was recorded. The highest number of cyanophages (>10(4) gene copy number per microliter) was observed in the period preceded by the following: an increase of the water retention time, growth of the water temperature, optimum nutrient concentrations, and the predomination of Microcystis bloom.

Development of toxin-producing cyanobacteria during the water level manipulation in a shallow heavily modified lake

Abstract The development of cyanobacteria and microcystin variation was studied during two years in response to periodical water-level regulation in modified Lake Tomaszne. Before the water entrance from a canal, the biomass of toxigenic cyanobacteria was 0.001-0.33 mg dm-3, the microcystin concentration was below 1 μg dm-3, and three variants of microcystins were detected. After the water entrance from the canal, the biomass of cyanobacteria increased to 1.3-7.1 mg dm-3 with the dominants Aphanizomenon gracile and Dolichospermum planctonicum. After the water discharge from the lake, Planktothrix agardhii reached the highest biomass (2.3-6.6 mg dm-3). During the mass development of toxigenic cyanobacteria, the total microcystin concentrations were mostly higher than 5 μg dm-3 and the number of MC variants increased. Both Pl. agardhii and D. planktonicum accounted for microcystin production. The higher NH4-N concentrations supplied with water from the canal supported the biomass increase of Nostocales, whereas Pl. agardhii mass development was due to the low light intensity and high TP concentrations. Our study revealed that the use of nutrient-rich waters for the water management in the hydromorphologically modified lake supported the persistent development of cyanobacteria species leading to increased amounts of MC and a higher number of their structural variants.