Total Tetrahydrocannabinol Research Articles

Evaluating Chinese fiber hemp (Cannabis sativa L.) varieties and planting dates in North Carolina

AbstractField trials were conducted in 2021 and 2022 to evaluate the effects of planting date (mid‐March, mid‐April, and mid‐May) on 11 fiber hemp (Cannabis sativa L. <0.3% total tetrahydrocannabinol) varieties. Trials were conducted in Goldsboro, Kinston, and Salisbury, NC. Each location followed a split‐plot randomized complete block design with at least three blocks where planting date was the main‐plot and variety the sub‐plot. Varieties investigated originated from China and Australia (2021 only). Data collection included flowering time, end of season stand counts, stem height, diameter, and final retted dry straw yield. We found differences among the varieties investigated in both years; however, no distinct trend was observed across years. All varieties investigated flowered at the end of August and beginning of September, allowing for a long growing season and ability to produce abundant biomass. In general, the Chinese genetics yielded higher stem biomass compared to previously reported European genetics. Stem thickness was >7.5 mm, which is generally considered the maximum width for textile‐grade fiber production. To achieve thinner stems from the varieties investigated, harvesting prior to male‐flower initiation may be required. The crop experienced temperatures below freezing in both years with no signs of damage. Taken together, farmers seeking to plant fiber hemp in North Carolina have a wide planting window from mid‐March to mid‐May using these Chinese varieties.

Exploring Chemical and Cultural Weed Management for Industrial Hemp Production in Georgia, USA

Industrial hemp (Cannabis sativa) production is complex, with strict regulatory constraints and challenges associated with a lack of labeled pesticides due to its status as a novel crop in the US. Four experiments were conducted in 2020 and 2021 to establish herbicide tolerances for hemp production in the coastal plain of Georgia, USA. Objectives included evaluating hemp response to pretransplant or posttransplant herbicides, determining if planting method influenced herbicide injury from residual preplant applied herbicides, and understanding how plastic mulch may influence hemp flower yields. When applied one day prior to transplanting, maximum hemp crop visual injury was less than 12% compared to the untreated control, with acetochlor, flumioxazin, fomesafen, pendimethalin, and norflurazon while dithiopyr, halosulfuron, isoxaben, and isoxaflutole resulted in greater than 50% injury. Posttransplant applications of S-metolachlor, acetochlor, pendimethalin, and clethodim resulted in less than 15% injury while halosulfuron, metribuzin, trifloxysulfuron, imazethapyr, and prometryn applications resulted in greater than 50% injury to plants. Preplant and posttransplant applied herbicides were found to have little effect on total tetrahydrocannabinol (THC), cannabidiol (CBD), or total cannabinoids in the dry flower after harvest. In a separate experiment, injury from halosulfuron and metribuzin was 52% to 56% less when planted with a mechanical transplanter as compared to the practice of using a transplant wheel to depress a hole in the soil followed by hand transplanting. In the final experiment, hemp dry flower yield in a non-plastic mulched (bareground) system was similar to that in a plastic mulched system. However, early season plant above-ground biomass was less in the plastic mulched system, which may have been due to elevated soil temperatures inhibiting early season growth.

Medicinal cannabis tea contains variable doses of cannabinoids and no terpenes.

Tea is a recommended way of administration of prescribed cannabis plant products in Denmark. We aimed to investigate the cannabinoid and terpene doses contained in different teas. We analysed tetrahydrocannabinol (THC), tetrahydrocannabinolic acid (THCA), cannabidiol (CBD), cannabidiolic acid (CBDA), and terpene concentrations in three repeated preparations of each type of tea, and in plant material. In standard tea, concentrations of THC were [median (min-max)] 9.5 (2.3-15), 19 (13-34), and 36 (26-57) μg/mL for products with a labelled content of 6.3%, 14%, and 22% total THC (THC + THCA), respectively. The CBD concentration in tea from a product labelled with 8% total CBD (CBD + CBDA) was 7.5 (1.9-10) μg/mL. Based on this, the recommended starting amount of 0.2L of the different teas would contain between 0.46 and 11.3mg THC, and 0.38 to 2.0mg CBD. Adding creamer before, but not after boiling, increased the THC and CBD concentration 2.3-4.4 and 2.1-fold, respectively. Terpenes were detected in plant material, but not in tea. The study elucidates THC and CBD doses in different teas, which may assist the clinician's choice of cannabis product. Moreover, it underscores the need for caution as administration as tea can result in exposure to different doses, even when the same cannabis product is used.

THC Content on a Dry Weight Basis: Implications for Hemp Legality.

The 2018 Farm Bill defines hemp as Cannabis sativa L. with a tetrahydrocannabinol (THC) concentration not more than 0.3% on a dry weight basis where THC is implied to be total THC (THCTotal) including both acid (Δ9-THCA) and neutral (Δ9-THC) forms. Effect of temperature and mass were studied to determine the quickest time to prepare fresh hemp suitable for grinding without affecting THCTotal. Proficiency testing program data were also analyzed to compare THCTotal contents on a dry versus wet weight basis. Fresh hemp of 150 and 400 g were freeze-dried or heat-treated at 50 °C, 60 °C, and 70 °C for various lengths of time up to 72 hours. Ground hemp was analyzed for moisture content via AOAC 934.01 and THCTotal via liquid chromatography/mass spectrometer (LC/MS) or gas chromatography with flame ionization detection (GC/FID). A data set analyzed from a proficiency testing program included moisture and THCTotal from 20 to 67 laboratories on 12 analytical samples circulated from 2020 through 2022. The quickest drying time of 24 h occurred at 70 °C with 150 g. These conditions did not affect THCTotal content on a dry weight basis. Twelve proficiency testing program analytical samples ranged from 5.8 to 11.4% moisture and 0.144 to 0.399% THCTotal on a wet weight basis. An increase in reported THCTotal on a dry weight basis compared to a wet weight basis ranged from 0.002 to 0.027% with a significant difference occurring in only one analytical sample. Suitable condition for drying hemp without altering THCTotal content was 24 h at 70 °C. Determining residual moisture in oven-dried ground hemp to calculate THCTotal content on a dry weight basis provides no benefit given minimal difference between contents on dry versus wet weight bases, interlaboratory variability, and terpene volatilization.

Compliance Testing of Hemp (Cannabis sativa L.) Cultivars for Total Delta-9 THC and Total CBD Using Gas Chromatography with Flame Ionization Detection.

The United States Agriculture Improvement Act passed in December of 2018 legalized the growing of Cannabis sativa containing not more than 0.3% total Delta-9 tetrahydrocannabinol (THC) in the country. While Cannabis sativa has been cultivated for hundreds of years, the illegal status of the plant in the United States, and elsewhere, has hindered the development of plant cultivars that meet this legal definition. To assess sampling strategies, and conformance to the THC limit, 14 cultivars of hemp were grown and tested by using gas chromatography with flame ionization detection for total delta-9 THC and total cannabidiol (CBD) during 2020, 2021 and 2022. Each year, samples of fresh plant material were collected from each cultivar weekly, beginning in mid-August and ending in late October, to examine the rate of increase in THC and CBD for different cultivars and select individual plants. The sampling demonstrated that both CBD and THC increase rapidly over a 1-2-week time frame with maximum concentrations (about 16% and 0.6%, respectively) around late September to early October. The testing of individual plants on the same day for select cultivars showed that while the ratio of CBD to THC remains constant (about 20:1 in compliant hemp) during the growing season, the individual plants are highly variable in concentration. Whereas previous studies have shown cultivar-dependent variability in THC production, this study demonstrated a novel plant-to-plant variability in the levels of THC within the same hemp cultivar. Understanding variability within and between hemp cultivars is useful to determine field sampling strategies and to assess the risk of crop embargoes to growers by compliance regulators.

Microwave-infrared drying of cannabis (Cannabis sativa L.): Effect on drying characteristics, energy consumption and quality

Conventional cannabis drying is time-consuming and energy intensive. A quick and dependable drying of cannabis is essential to ensure high-quality products to meet increasing demands. This study explored a combined microwave and infrared (MI) drying on cannabis comparing with control environmental (CE) drying. MI was very efficient with a short drying time of 16–200 min, high moisture diffusivity of 7.95×10−09-8.70×10−08 m2/s, and low energy consumption of 390.49–1611.42 kJ. The Page and Modified Page drying models fitted well to describe and predict the MI drying characteristics of cannabis. Microstructural images identified shrinkage in glandular trichomes of cannabis, whereas colorimetric assessment resulted in alteration of color attributes due to MI drying. It also facilitated the conversion of acidic cannabinoids to their neutral forms by decreasing tetrahydrocannabinolic acid (THCA) in g/g of dry matter from 20.15% to 7.57% and increasing tetrahydrocannabinol (THC) from 6.31% to 16.65% that insignificantly (p≥0.05) affected the total THC level. MI drying resulted in a total terpenes concentration (%g/g of dry matters) of 0.541–0.730, insignificantly lower than CE drying (0.768). Overall, the study highlights MI as a rapid and energy-efficient drying for obtaining high quality cannabis, particularly for medicinal applications.

Potency levels of regulated cannabis products in Michigan 2021-2022.

Evaluation of cannabinoid concentrations in products from the legal cannabis market has been fraught with uncertainty. The lack of standardized testing methodology and the susceptibility of cannabinoids to degradation under certain storage conditions complicates the efforts to assess total tetrahydrocannabinol (THC) levels across wide geographic areas. There are few peer-reviewed surveys of cannabinoid concentrations in regulated products. Those that have been done have not characterized the effects of differences in analytical methodology, sample population, and storage conditions. Viridis Laboratories, which operates two cannabis safety compliance facilities in Michigan, has analyzed over 34,000 cannabis products throughout 2021 and 2022 before the sale in the regulated market. Fifteen cannabinoids in cannabis flower, concentrates, and infused products were tested using methanolic extraction and analysis by high-performance liquid chromatography with diode-array detection. Methods were validated before use, and the flower analysis procedure was certified by the Association of Analytical Collaboration. All the samples were tested before submission for sale and therefore had not undergone prolonged storage. The results are compared with those seen in other states as well as in the illicit market. Total THC levels in cannabis flower from the regulated market are significantly higher than those seen in illicit products. The distribution of cannabinoid levels is similar in flowers intended for either the medicinal or adult-use markets, with an average potency of 18%-23% of total THC. Total THC in concentrates averages up to 82%. Other cannabinoids are observed at significant levels, mostly in products specifically formulated to contain them. These results may act as a benchmark for potency levels in the regulated market.

Assessing the impact of piercing-sucking pests on greenhouse-grown industrial hemp (Cannabis sativa L.).

Cannabis sativa or hemp, defined as <0.3% total tetrahydrocannabinol (THC), is a specialty crop in the United States, of particular interest among growers in the southeastern United States to replace tobacco production. Tetranychus urticae (twospotted spider mite), Aculops cannabicola (hemp russet mite), Polyphagotarsonemus latus (broad mites), and Phorodon cannabis (cannabis aphids) are considered the most significant pests in greenhouse grown hemp. Mite and aphid injury can cause cupping and yellowing of leaves, resulting in leaf drop, and reduced flower and resin production. We sought to understand the effects of feeding by T. urticae and Myzus persicae (green peach aphid), as a proxy for P. cannabis, on the concentration of economically significant cannabinoids through a series of experiments on greenhouse grown plants. First, we compared the variability of chemical concentrations in samples collected from individual plants versus pooled samples from 5 plants, and found that chemical concentrations in single plants were similar to those in pooled plant samples. Next, we compared chemical concentrations prior to arthropod infestation and post infestation. When evaluating the mite feeding damage in 2020, cannabinoids in plants infested with high densities of T. urticae increased more slowly than in uninfested control plants or plants infested with low T. urticae densities. In 2021, the concentration of tetrahydrocannabinol did not differ significantly between treatments. Cannabidiol increased more slowly in plants with low T. urticae densities when compared to uninfested controls but did not differ from the high T. urticae densities 14 days after infestation.

Hemp yield and cannabinoid concentrations under variable nitrogen and potassium fertilizer rates

AbstractWith the passing of the 2014 US farm bill, there is more interest in industrial hemp (Cannabis sativa L. &lt; 0.3% total tetrahydrocannabinol [THC]) grown for cannabinoid production. However, production recommendations that outline fertilizer requirements of cannabidiol (CBD) hemp do not exist. Our primary objective was to identify nitrogen (N) and potassium (K) fertilizer rates for maximizing biomass and CBD yield. A secondary objective was to identify the relationships between N and K rates and total THC and total CBD concentrations. Fertilizer rates from 0 to 224 kg N and 0 to 185 kg K ha−1 were tested separately at four general locations in North Carolina. Two locations were used in 2019 and 2020, while the other two were used only in 2020. Dry weight yield was predicted to increase linearly from 1822 to 3384 kg biomass ha−1 as N rate increased from 0 to 86.8 kg ha−1. Nitrogen rates above 86.8 kg ha−1 were not predicted to increase biomass. Likewise, as N rate increased from 0 to 84.2 kg N ha−1, CBD yield was predicted to increase linearly from 204 to 389 kg CBD ha−1. Additional N was not predicted to increase CBD yield. The CBD and THC concentrations showed a slight bell‐shaped response curve over increasing N rates, ranging from 11.33% to 12.11% and 0.473% to 0.509%, respectively. Potassium application did not affect yield nor CBD and THC concentrations. Results from this work indicate that N is a more limiting factor than K for maximizing CBD hemp biomass production.

Medical cannabis use among patients with Post-Traumatic Stress Disorder (PTSD): A nationwide database study

IntroductionIn recent years, cannabis use among PTSD patients has become more common than ever. However, data available today regarding the effectiveness and safety of medical cannabis in PTSD treatment is limited, based on cross sectional studies, self-report surveys and a few clinical studies with small sample size.ObjectivesTo characterize patterns of use and adverse effects over time in patients with PTSD using medical cannabis in real life setting.MethodsData were acquired from the Israeli national database of all patients licensed to use medical cannabis from January 2014 to December 2021. A license for medical cannabis is given to patients with PTSD of at least moderate intensity after treatment failure of at least two drugs and two psychological interventions. Comparative statistics were used to evaluate patterns of use and adverse effects.Results12,977 patients were licensed to use medical cannabis in the study period for PTSD (8.2% of all users; 70% men) during the above-mentioned time period. PTSD was the 3rd most common indication after chronic pain and symptoms related to oncological disease and chemotherapy treatment. Over time, the relative increase in use of medical cannabis among PTSD patients was higher than that found in non-PTSD patients. In 2021 36.2% of all PTSD patients using medical cannabis had their license issued that year compared to 28.1% of all non-PTSD patients. PTSD patients were significantly younger compared to non-PTSD patients (40.9 years vs. 52.9 years). PTSD patients consume slightly higher monthly amount at the beginning of treatment compared to non-PTSD patients (32.1gr vs. 30.6gr) with higher Tetrahydrocannabinol (THC) concentration (15.2% vs. 12.9%) and lower Cannabidiol (CBD) concentration (4.7% vs. 6.0%). Over two years of use, amount, and composition of cannabis among the two groups were comparable and showed an increase in total amount and THC concentration, reaching the maximal available THC concentration of 20%. Data regarding adverse effects were available for 6,242 PTSD patients (48.1%) and 39,497 non-PTSD patients (26.6%). PTSD patients reported more physical adverse effects (RR 1.45 [95%CI 1.34-1.56]), anxiety (RR 1.47 [95%CI 1.13-1.92]), and derealization (RR 3.44 [95%CI 2.42-4.89]).ConclusionsPTSD is one of the leading indications for medical cannabis use in Israel, despite scarcity in good quality data supporting its effectiveness and safety. The increased risk of mental adverse effects among PTSD patients emphasizes the need for cautious use in cannabis in this population. Expanding the knowledge regarding patterns of use and risks in medical cannabis use among PTSD patients is important for understanding the role of cannabis in PTSD treatment and to ensure an effective and safe treatment.Disclosure of InterestNone Declared

State Variation in U.S. Medical Cannabis Limits, Restrictions, and Therapeutic Cannabis Dosing.

Wide variation exists in the possession limits of cannabis products sold for medical use in the U.S. as well as the tetrahydrocannabinol (THC) content of cannabis products. Prior work has found that legal limits on recreational cannabis sold per transaction may promote moderate use and diversion. This paper finds similar results for monthly medical cannabis limits. In the present analyses, state limits on medical cannabis were aggregated and converted into 30-day limits and 5 milligram (mg) THC doses. Grams of pure THC were calculated using plant weight limits and medical cannabis median THC potency aggregated from Colorado and Washington state medical cannabis retail sales data. Weight in pure THC was then broken down into 5 mg doses. Weight-based possession limits of cannabis for medical use varied widely across states (range: 1.5-762.05 grams pure THC per 30 days), with three states lacking a quantifiable weight limit (in which limits are not by weight but by physician's recommendation). States generally do not impose limits on the potency of cannabis products, therefore small differences in weight limits can result in large differences in the amount of total THC allowed to be sold. Assuming a typical medical dose of 5 mg and the median THC potency of 21%, current laws allow for sales of 300 (Iowa) to 152,410 (Maine) doses per month. Current state statutes and methods of cannabis recommendation allow patients to increase therapeutic THC doses independently, and perhaps unknowingly. High THC content products combined with the higher purchase or possession limits allowed by medical cannabis laws may lead to an increased potential for overconsumption or diversion.

The Effect of Harvest Date on Temporal Cannabinoid and Biomass Production in the Floral Hemp (Cannabis sativa L.) Cultivars BaOx and Cherry Wine

The objectives of this study were to model the temporal accumulation of cannabidiol (CBD) and tetrahydrocannabinol (THC) in field-grown floral hemp in North Carolina and establish harvest timing recommendations to minimize non-compliant crop production. Field trials were conducted in 2020 and 2021 with BaOx and Cherry Wine cultivars. Harvest events started two weeks after floral initiation and occurred every two weeks for 12 weeks. Per-plant threshed biomass accumulation exhibited a linear plateau trend. The best fit model for temporal accumulation of THC was a beta growth curve. As harvest date was delayed, total THC concentrations increased until concentrations reached their maximum, then decreased as plants approached senescence. Logistic regression was the best fit model for temporal accumulation of CBD. CBD concentrations increased with later harvest dates. Unlike THC concentrations, there was no decline in total CBD concentrations. To minimize risk, growers should test their crop as early as possible within the USDA’s 30-day compliance window. We observed ‘BaOx’ and ‘Cherry Wine’ exceeding the compliance threshold 50 and 41 days after flower initiation, respectively.

Variation among hemp (Cannabis sativus L.) analytical testing laboratories evinces regulatory and quality control issues for the industry

Validated analytical testing of cannabinoid content for regulatory purposes is critical to farming high-cannabidiol (CBD) hemp (Cannabis sativus L.), as these methods are key to determine whether a crop is federally compliant by containing < 0.3% THC or must be destroyed at the time of harvest. This report identifies the sources and extent of variation in reported cannabinoid content after flower selection and conducted a survey of ten accredited hemp regulatory testing laboratories, including one state-owned laboratory. The results indicate that total tetrahydrocannabinol (THC) content is reported inconsistently due to an insufficient standardization of sample preparation and testing methods, thus likely leading to erroneous data. This work represents the early phase of ongoing research to enhance the consistency of cannabinoid analyses of hemp flower samples as an essential tool in Cannabis crop development.

Different Fertility Approaches in Organic Hemp (Cannabis sativa L.) Production Alter Floral Biomass Yield but Not CBD:THC Ratio

Industrial hemp is once again legal in the United States but agronomic practices are not well characterized, especially for organic production systems. Many producers are concerned that while increased fertility may increase biomass yield it will also disproportionally increase that rate of total tetrahydrocannabinol (THC) accumulation, making their crop more likely to be noncompliant at harvest. We measured the effect of four organic fertility treatments: (1) no fertility (NA); (2) manure-based compost (CM); (3) an industry-standard organic fertilizer blend (ST); and (4) an intensive fertility treatment designed for cannabis production (BQ), on biomass yield and cannabinoid accumulation over multiple timepoints and two years. We found that higher fertility (ST and BQ) led to increased biomass but did not influence the ratio of cannabidiol (CBD) to THC over time. We also found the ST treatment yielded the most consistently across years, whereas CM and BQ were less predictable and more costly, respectively. Our results show that fertility recommendations can be made to increase biomass yield without increasing the chance of noncompliance. Additional research addressing specific fertility requirements in organic cropping systems for hemp will be needed to meet consumer demand while remaining profitable and sustainable for growers.

Studying the Effects of Waterhemp Competition on Yield and Phytochemical Content of High-cannabidiol Hemp in Plasticulture

The impacts of weed interference on hemp (Cannabis sativa) yield are largely unstudied despite causing serious economic losses in most cropping systems. For high-cannabidiol (CBD) hemp, understanding the role of weed competition on CBD and tetrahydrocannabinol (THC) content may help promote profitability and regulatory compliance. Therefore, we tested the effects of varying waterhemp [Amaranthus tuberculatus (zero, one, three, and five waterhemp plants per planting hole)] and hemp (zero or one hemp plants per planting hole) planting densities on total hemp yield, chemical composition, and aboveground waterhemp biomass in plasticulture. There was no significant total biomass or stripped floral biomass yield loss resulting from waterhemp competition, although unexpectedly high variation in hemp phenotypes likely limited the ability to detect subtle differences between treatments. Furthermore, there was no significant effect of competition treatment on total CBD, total THC, or measured terpene composition. However, waterhemp biomass was reduced significantly by competition from hemp in comparison with hemp-free treatments. Suppression of waterhemp by hemp and lack of significant yield loss suggest that hemp can be highly competitive and grown successfully without herbicides in certain circumstances.

How do pinching and plant density affect industrial hemp produced for cannabinoids in open field conditions?

AbstractInformation regarding agronomic practices for field‐scale industrial hemp (IH) production for cannabinoids is extremely limited. The objective of this study was to investigate how pinching practice (i.e., removing the apical meristem) and varied plant density affect yield and cannabinoid production of IH in open field conditions. A field trial was performed in northern Florida in 2019 and 2020. The pinching study included pinch and non‐pinch treatments evaluated on two commercially available day‐length–sensitive IH cultivars Cherry Blossom (CBL) and Cherry Wine (CW). Four plant densities (3,000, 4,000, 6,000, and 12,000 plants ha–1) were evaluated on CW. The experimental design was a randomized complete block design with four blocks. Total flower yield in 2019 was two times greater than in 2020. In 2020, total tetrahydrocannabinol and cannabidiol (CBD) in CBL was significantly greater than in CW. Pinching did not significantly improve flower yield, cannabinoid concentration, or CBD production. Flower yield per plant decreased as plant density increased, but a reversed trend occurred for total flower yield per hectare. Cannabinoid concentration was not affected by plant density, but CBD production increased with increased plant density. Due to the high cost of IH plant material and labor, greater plant density does not always guarantee greater economic return. This study provides valuable information for field‐scale cultivation of IH for cannabinoids and supports the development of a large agricultural industry globally.

A comparative study of supercritical fluid and ethanol extracts of cannabis inflorescences: Chemical profile and biological activity

We compared chemical profiles and in vitro biological activity of extracts obtained through maceration with ethanol or supercritical fluid extraction with CO2 (scCO2), from two different cannabis inflorescences chemotypes, one with high total tetrahydrocannabinol (THC) and other with high total cannabidiol (CBD) content. scCO2 was performed to obtain extracts with highest total THC and CBD recovery using ethanol as a co-solvent. In addition, a short maceration with ethanol was performed for both chemotypes. Cannabinoid and terpene composition of the extracts were determined using chromatographic and spectroscopic methods, and their cytotoxicity and cell proliferation effects were evaluated in vitro using HT-29 colon adenocarcinoma cell line. In vitro biological activity confirmed that cannabis extracts from both chemotypes have anti-proliferative activity but the scCO2 extract from high total THC chemotype was more cytotoxic. These results underscore the importance of chemotype and extraction method selection in the preparation of cannabis extracts for different therapeutic applications.

Identification of Chemotypic Markers in Three Chemotype Categories of Cannabis Using Secondary Metabolites Profiled in Inflorescences, Leaves, Stem Bark, and Roots.

Previous chemotaxonomic studies of cannabis only focused on tetrahydrocannabinol (THC) dominant strains while excluded the cannabidiol (CBD) dominant strains and intermediate strains (THC ≈ CBD). This study investigated the utility of the full spectrum of secondary metabolites in different plant parts in three cannabis chemotypes (THC dominant, intermediate, and CBD dominant) for chemotaxonomic discrimination. Hierarchical clustering, principal component analysis (PCA), and canonical correlation analysis assigned 21 cannabis varieties into three chemotypes using the content and ratio of cannabinoids, terpenoids, flavonoids, sterols, and triterpenoids across inflorescences, leaves, stem bark, and roots. The same clustering results were obtained using secondary metabolites, omitting THC and CBD. Significant chemical differences were identified in these three chemotypes. Cannabinoids, terpenoids, flavonoids had differentiation power while sterols and triterpenoids had none. CBD dominant strains had higher amounts of total CBD, cannabidivarin (CBDV), cannabichromene (CBC), α-pinene, β-myrcene, (−)-guaiol, β-eudesmol, α-eudesmol, α-bisabolol, orientin, vitexin, and isovitexin, while THC dominant strains had higher total THC, total tetrahydrocannabivarin (THCV), total cannabigerol (CBG), camphene, limonene, ocimene, sabinene hydrate, terpinolene, linalool, fenchol, α-terpineol, β-caryophyllene, trans-β-farnesene, α-humulene, trans-nerolidol, quercetin, and kaempferol. Compound levels in intermediate strains were generally equal to or in between those in CBD dominant and THC dominant strains. Overall, with higher amounts of β-myrcene, (−)-guaiol, β-eudesmol, α-eudesmol, and α-bisabolol, intermediate strains more resemble CBD dominant strains than THC dominant strains. The results of this study provide a comprehensive profile of bioactive compounds in three chemotypes for medical purposes. The simultaneous presence of a predominant number of identified chemotype markers (with or without THC and CBD) could be used as chemical fingerprints for quality standardization or strain identification for research, clinical studies, and cannabis product manufacturing.

A Preliminary Study: The C-Vue Chromatograph as a Presumptive Tool to Assist Kansas Law Enforcement to Distinguish Between Legal and Illegal Forms of Cannabis

Since the federal government now controls cannabis based on the total tetrahydrocannabinol (THC) content of 0.3% by dry weight, law enforcement, particularly in states in which cannabis is still controlled, faces many new challenges regarding the potential seizure of cannabis. No longer is the traditional Duquenois-Levine color reaction test utilized by law enforcement for many years able to distinguish between legal versus illegal forms of cannabis; both federally legal and illegal cannabis contain cannabinoids, which produce positive responses for this presumptive test. Law enforcement officers often need a quick answer when it comes to deciding whether or not to seize potential cannabis without the fear of a potential lawsuit. They often do not have the time to send a sample to an analytical lab and wait weeks for the results. This study evaluated the C-Vue Liquid Chromatograph (LC) as a simple, robust, affordable presumptive tool that, within less than an hour, allows officers to determine whether the sample is most likely legal (hemp) or illegal cannabis (marijuana) based on the 0.3% THC threshold. When this data was compared against a validated research-grade LC, using a set of 50 cannabis samples from different sources, the C-Vue correctly predicted whether the THC concentration was above or below the 0.3 wt. % threshold in 48 out of 50 samples, suggesting that the C-Vue may be useful as a presumptive tool.

Effect of Cannabis sativa L. root, leaf and inflorescence ethanol extracts on the chemotrophic response of entomopathogenic nematodes

Soils represent the natural habitat of entomopathogenic nematodes (EPNs). When moving in soil, EPNs are oriented to follow a chemical signal (chemotaxis). Cannabis sativa L. is known to secrete a very wide spectrum of secondary metabolites. Ethanol extracts (EE) of different C. sativa L. organs were used to study the effect on EPN chemotaxis. The root, leaf and inflorescence EE of two hemp varieties and two medical cannabis breeding lines were used in laboratory chemotaxis assays with three EPN species (Steinernema carpocapsae, S. feltiae and Heterorhabditis bacteriophora) at 20 and 25 °C. The content of terpenes and cannabinoids in C. sativa L. inflorescences was measured as well. Overall, EPNs were most attracted or repelled by inflorescence extracts, followed by leaves and roots. The most abundant terpene in hemp inflorescences was trans caryophyllene (38.2% on average), the highest contents of total cannabidiol, total cannabigerol and total tetrahydrocannabinol were 9.65%, 0.89% and 0.62%, respectively. The attraction effect of S. sativa L. EE is a highly interesting outcome and could lead to the development of attractant compounds in EPN biological control. Cannabinoids and terpenes may be responsible for pronounced effects on EPN chemotaxis.