Abstract Enhancing soybean production can be achieved through plant breeding, utilizing gamma ray radiation mutation techniques to develop superior varieties. Understanding radiosensitivity is crucial for comprehending the impact of radiation on plant morphology and physiology. This study aims to investigate the impacts of varying doses and dose rates of Cobalt-60 gamma radiation on the growth and development of two soybean varieties, Grobogan and Mutiara 1. This study was conducted to understand how varied radiation doses influence growth parameters and to determine the radiosensitivity, LD50 value, in mutants of the Grobogan and Mutiara 1 soybean varieties. The study employed a randomized complete design with two factors. The first factor was radiation dose ranging from 0 to 1000 Gy. The second factor was dose rate, categorized into low dose rate (219.5 Gy/hour) and high dose rate (4470.2 Gy/hour). The results revealed that the LD50 value of Grobogan variety was estimated at 588.56 Gy at a low dose rate and 657.17 Gy at a high dose rate, while the Mutiara 1 variety had an LD50 value estimate of 512.36 Gy at a low dose rate and 514.30 Gy at a high dose rate. The study also found that the gamma ray radiation dose in the seedling phase trial influenced several growth parameters, including germination percentage, seedling height, development of stem nodes, and formation of leaves. Furthermore, field phase trial showed that a range of radiation dose at 100-300 Gy led to an increase in the number of branches in both soybean varieties and plant height in the Grobogan variety. It also resulted in an increase in the production of pods and total weight of seeds in the Mutiara 1 variety. These findings offer valuable insights into optimizing radiation doses for increasing genetic diversity in soybean.