Total Psi Research Articles

Dehydration of bioethanol using novel polysulfone (PSF)/nonionic surfactants hybrid membranes

This study focuses on developing a novel polysulfone (PSF) dense hybrid membrane modified with nonionic surfactants, Tween 20 (T20) and Triton X-100 (X100), for improved pervaporation (PV) dehydration of bioethanol. The modified membranes were then examined for their ethanol dehydration performance, microscopy and spectroscopy characteristics, surface charge, mechanical and roughness properties, crystallinity structure, differential scanning calorimetry (DSC), swelling, and contact angle (CA) analysis. The free volume structure of the membranes was assessed using positron annihilation lifetime spectroscopy (PALS) and Doppler broadening spectroscopy (DBS). A significant discovery from this analysis was the semi antiplasticizing effect observed when surfactants were added to the PV membranes. The PV results revealed that incorporating 0.5 wt% T20 into PSF significantly improved the total flux and PSI of PSF/T20 to 226.35 g/m2 h and 46578 g/m2 h, respectively. Similarly, the total flux and PSI of hybrid membranes containing X100 increased to 110.19 g/m2 h and 43680 g/m2 h. Consequently, the developed PSF/T20 membranes demonstrated more significant potential for bioethanol dehydration compared to PSF/X100 membranes.

Incidence and predictors of posttraumatic epilepsy and cognitive impairment in patients with traumatic brain injury: A retrospective cohort study in Malaysia.

Posttraumatic epilepsy (PTE) significantly impacts morbidity and mortality, yet local PTE data remain scarce. In addition, there is a lack of evidence on cognitive comorbidity in individuals with PTE in the literature. We sought to identify potential PTE predictors and evaluate cognitive comorbidity in patients with PTE. A 2-year retrospective cohort study was employed, in which adults with a history of admission for traumatic brain injury (TBI) in 2019 and 2020 were contacted. Three hundred one individuals agreed to participate, with a median follow-up time of 30.75 months. The development of epilepsy was ascertained using a validated tool and confirmed by our neurologists during visits. Clinical psychologists assessed the patients' cognitive performance. The 2-year cumulative incidence of PTE was 9.3% (95% confidence interval [CI] 5.9-12.7). The significant predictors of PTE were identified as a previous history of brain injury [hazard ratio [HR] 4.025, p = .021], and intraparenchymal hemorrhage (HR: 2.291, p = .036), after adjusting for other confounders. TBI patients with PTE performed significantly worse on the total ACE-III cognitive test (73.5 vs 87.0, p = .018), CTMT (27.5 vs 33.0, p = .044), and PSI (74.0 vs 86.0, p = .006) than TBI patients without PTE. A significantly higher percentage of individuals in the PTE group had cognitive impairment, compared to the non-PTE group based on ACE-III (53.6% vs 46.4%, p = .001) and PSI (70% vs 31.7%, p = .005) scores at 2 years post-TBI follow-up. This study emphasizes the link between TBI and PTE and the chance of developing cognitive impairment in the future. Clinicians can target interventions to prevent PTE by identifying specific predictors, which helps them make care decisions and develop therapies to improve patients' quality of life.

Parental stress and food allergy phenotypes in young children: A National Birth Cohort (JECS).

Food allergy children and their families tend to have emotional distress and anxiety. There have been few reports of differences in parenting stress and a child's food allergy phenotypes. We examined the associations between food allergy phenotypes in children and parenting stress assessed by the Parenting Stress Index-Short Form (PSI-SF) from a national birth cohort (Japan Environment and Children's Study). We included 65,805 children for statistical analysis. Of them, 7.2% of children had a food allergy diagnosis at 2 years old. The means of the total PSI-SF (39.9 ± 10.3, 39.1 ± 9.9), CD-SF (19.5 ± 5.4, 19.1 ± 5.2), and PD-SF (20.5 ± 6.3, 20.0 ± 6.1) scores are similar for caregivers in the with and without food allergy groups. Food allergy diagnosis resulted in significantly higher total PSI scores (coefficient .47, 95% CI 0.19-0.75, p = .001), CD-SF (coefficient .22, 95% CI 0.07-0.38, p = .004), and PD-SF (coefficient .24, 95% CI 0.08-0.41, p = .004). A similar trend was observed for allergy reactions to hen's egg. However, there was no clear relationship between allergic reactions to milk, wheat, nuts, and PSI-SF. Parental stress was significantly related to a child's food allergy. Furthermore, hen's egg allergy increased parental stress. Multiple food avoidance might also increase parental stress. Healthcare providers need to be aware of parental stress in our daily clinic.

The effect of ketamine and D-cycloserine on the high frequency resting EEG spectrum in humans

RationalePreclinical studies indicate that high-frequency oscillations, above 100 Hz (HFO:100–170 Hz), are a potential translatable biomarker for pharmacological studies, with the rapid acting antidepressant ketamine increasing both gamma (40–100 Hz) and HFO.ObjectivesTo assess the effect of the uncompetitive NMDA antagonist ketamine, and of D-cycloserine (DCS), which acts at the glycine site on NMDA receptors on HFO in humans.MethodsWe carried out a partially double-blind, 4-way crossover study in 24 healthy male volunteers. Each participant received an oral tablet and an intravenous infusion on each of four study days. The oral treatment was either DCS (250 mg or 1000 mg) or placebo. The infusion contained 0.5 mg/kg ketamine or saline placebo. The four study conditions were therefore placebo-placebo, 250 mg DCS-placebo, 1000 mg DCS-placebo, or placebo-ketamine.ResultsCompared with placebo, frontal midline HFO magnitude was increased by ketamine (p = 0.00014) and 1000 mg DCS (p = 0.013). Frontal gamma magnitude was also increased by both these treatments. However, at a midline parietal location, only HFO were increased by DCS, and not gamma, whilst ketamine increased both gamma and HFO at this location. Ketamine induced psychomimetic effects, as measured by the PSI scale, whereas DCS did not increase the total PSI score. The perceptual distortion subscale scores correlated with the posterior low gamma to frontal high beta ratio.ConclusionsOur results suggest that, at high doses, a partial NMDA agonist (DCS) has similar effects on fast neural oscillations as an NMDA antagonist (ketamine). As HFO were induced without psychomimetic effects, they may prove a useful drug development target.

The Effect of Group Counseling in Reducing Parental Stress and Depression in Jordanian Mothers of Children with Autism

This study aimed to investigate the impact of a group counseling program on reducing parental stress and depression among mothers of Jordanian children with autism. A purposive sample comprising 40 mothers of children with autism was selected from three autism centers in Zarqa and Rusaifa in Jordan; these participants were then randomly assigned to either an experimental group (N = 20) or control group (N = 20). Participants were asked to complete both the Parental Stress Index–Short Form (PSI–SF; including its three sub-scales) and an amended Arabic version of the Depression, Anxiety and Stress Scale (DASS): pre- and posttest by both groups, and a six-week follow-up test by the experimental group. A CBT-based group counseling program was designed and 14 sessions were held three times per week over a four-week period. The difference between the pre- and posttest scores for the two groups revealed that the experimental group was significantly more likely to have lower levels of parental stress and depression. Furthermore, there was a significant difference in scores between the experimental group’s posttest and follow-up assessment: Total Stress (Total PSI), Parental Distress (PD), and depression had all decreased significantly; Parent–Child Dysfunctional Interaction (PCDI) had also decreased, but not significantly; however, although not significantly, stress levels associated with a Difficult Child (DC) had increased.

Parenting stress trajectories in mothers of very preterm infants to 2 years

ObjectiveTo examine levels of parenting stress in mothers of preterm and term infants when the children were 2 years old; to determine the trajectory of stress over three time periods...

Effect of Nursing Intervention Program on stress and competence of Parents of Attention Deficit Hyperactivity Disorders Children

Background: The Attention-Deficit/Hyperactivity-Disorder (ADHD) is a common neurobehavioral childhood problem with negative impacts on child and parents. The aim of this study was to evaluate the effect of a nursing intervention program on stress and competence of parents of ADHD children. Subjects M child-related, parent-related, and total PSI (p<0.001). ADHD improved among 78% after the intervention (p<0.001). Statistically significant negative correlations were found between the scores of parents’ satisfaction, competence, and PSOC from one side and each of the child-related, parent-related, PSI, and ADHD scores. In conclusion, the implementation of an educational intervention to the parents of children with ADHD is effective in improving their parenting satisfaction, efficacy, and PSOC, with reduction in their stress, and improvement of child abnormal behaviors. Recommendations: It is recommended to apply the developed program in the study settings for more improvements. The school health team needs to be trained in screening and early detection, with counseling clinics for parents of ADHD children.

Sensory subtypes and associated outcomes in children with autism spectrum disorders.

Sensory features are prevalent and heterogeneous across children with ASD and these features have been associated with child outcomes. Identification of clinically defined sensory subtypes may enhance our understanding of unique phenotypes that have implications for etiology, prognosis, and intervention. This longitudinal study used a national online survey aimed to identify associations of previously validated sensory subtypes to specific child and family characteristics and functional outcomes [vineland adaptive behavior scale-II (VABS) and parenting stress index short form (PSI)]. The sensory experiences questionnaire-3.0 was collected from caregivers with children with ASD, ages 2-12, at two time points (Time 1, n = 1307, Time 2, n = 884), 1 year apart. Functional outcomes assessments were collected at the second time point. A latent profile transition analysis (LPTA) was used to test associations, and results indicated that the attenuated-preoccupied subtype presented with the significantly lowest levels of VABS adaptive behavior composite scores compared to the other three sensory subtypes. Both the VABS maladaptive behavior index and the total PSI score were significantly highest in the extreme-mixed subtype. These results underscore the clinical utility of this subtyping approach for differentiating characteristics and functional outcomes associated with clinically defined sensory phenotypes. These findings may have implications for better understanding etiology, prognosis, and more precise targets for interventions designed to ameliorate sensory difficulties, and ultimately mitigate negative developmental consequences and parenting stress. Autism Res 2016, 9: 1316-1327. © 2016 International Society for Autism Research, Wiley Periodicals, Inc.

Association between Left Ventricular Postsystolic Shortening and Diastolic Relaxation in Asymptomatic Patients with Systemic Hypertension.

Few data have existed on myocardial postsystolic shortening (PSS) accounting for left ventricular (LV) diastolic dysfunction in systemic hypertension. We examined this relationship, along with clinical and other cardiac parameters, in asymptomatic patients with hypertension. We analyzed 104 patients on pharmacological treatment for hypertension who had no signs or symptoms of heart failure for conventional and speckle tracking echocardiography. The sum of the postsystolic index for multiple LV segments (total PSI) and global longitudinal strain (GLS) was computed and used as measures for global PSS and myocardial systolic function, respectively. The tissue Doppler e' and the speckle tracking-derived global strain rate during early diastole (e'sr) were obtained as indicators of LV relaxation. The circumferential end-systolic stress was also determined noninvasively and substituted for a measure of myocardial afterload. Main variables that correlated with the e' and e'sr were shown to be age, LV mass index, left atrial volume index, GLS, and the total PSI. Multivariate analysis including gender, circumferential end-systolic stress, and the use of calcium channel blockers as possible covariates revealed that age (β=-0.29, P=0.002), total PSI (β=-0.26, P=0.008), and LV mass index (β=-0.25, P=0.017) were significant independent determinants of e' and that age (β=-0.34, P<0.001) and GLS (β=-0.28, P=0.006) were of e'sr. Our findings indicate that while depending on the degree of GLS, PSS may affect diastolic relaxation in patients with asymptomatic, but treated, hypertensive patients.

Characterization and Pervaporation Properties of Modified PU Membranes

Pervaporation will have broad application prospects in the treatment of phenolic wastewater. Polyurethane (PU) membrane is promising but needs to be further modified. In this study, both β-CD and CNT were used to modify the PU membrane. The structure and property of these membranes were characterized by FTIR, DSC and TGA. The pervaporation performances were investigated, and the effects of β-CD and CNT on membrane separation were discussed. TGA shows that three kinds of modified membranes have enough thermal stability to afford pervaporation test and start weight loss at about 200 °C. In pervaporaiton, the total flux, separation factor and PSI value all increase with increased feed temperature. That is anti-trade-off phenomenon. Among these membranes, HTPB+β-CD-PU shows the best comprehensive performance at 80°C and has great potential for further application.

Die kognitive Entwicklung von Drillingen im Schulalter und ihr Einfluss auf die Qualität des Familienlebens – eine Follow-up Studie eines Perinatalzentrums

The aim of this study was to assess the developmental outcome of 7- to 9-year-old triplets and to determine parenting stress and quality of family life. Cognitive development (Wechsler intelligence scales for children III, WISC III) and quality of family life (Kansas family life satisfaction scale, KFLSS; parenting stress index, PSI) of 48 triplets born between 1996 and 1998 at a perinatal centre were compared with controls born at a gestational age ≥37 weeks. Index and control children/mothers were matched by age (birthday: ±14 days/maternal age±2 years). Triplets and their families showed a mean IQ score and parenting satisfaction and stress within the normal range. Full IQ score (97 SD 16) as well as verbal and performance IQs (102 SD 16; 92 SD 19) were significantly lower than the controls' corresponding data (full IQ 111 SD 15, p<0.001; verbal IQ 116 SD 21, p<0.001; performance IQ 102 SD 17, p=0.001). Parents of triplets tended to show a higher total PSI score (222 SD 39) than the parents of the controls (205 SD 47). The differences reached statistical significance only in the parent domain (triplets 125 SD 24; controls 111 SD 27; p<0.01). Satisfaction with family life was good in triplet families and did not differ significantly in any of the subscales from control parents' satisfaction. This study demonstrates a favourable outcome of triplets with respect to their cognitive development and the quality of their families' lives at school age. The difference in children's IQ was statistically, but perhaps not clinically, significant, because the triplets' data were within the normal range.

Heavy-quarkonium production in high energy proton-proton collisions at RHIC

We update the study of the total Psi and Upsilon production cross section in proton-proton collisions at RHIC energies using the QCD-based Color-Singlet (CS) Model, including next-to-leading order partonic matrix elements. We also include charm-quark initiated processes which appear at leading order in alpha_s, but which have so far been overlooked in such studies. Contrary to earlier claims, we show that the CS yield is consistent with measurements over a broad range of J/Psi rapidities. We also find that charm-quark initiated processes, including both intrinsic and sea-like charm components, typically contribute at least 20% of the direct J/Psi yield, improving the agreement with data both for the integrated cross section and its rapidity dependence. The key signature for such processes is the observation of a charm-quark jet opposite in azimuthal angle phi to the detected J/Psi. Our results have impact on the proper interpretation of heavy-quarkonium production in heavy-ion collisions and its use as a probe for the quark-gluon plasma.

Technology Development Highlights Generated from the Center for Eukaryotic Structural Genomics

The Center for Eukaryotic Structural Genomics (CESG) aims to be the leading center for developing and disseminating tested technologies to efficiently solve structures of eukaryotic proteins. We create, evaluate, and optimize innovative protocols for producing eukaryotic proteins in active forms. We seek to improve the efficiency of all stages from target selection-design to three-dimensional structure determination by X-ray crystallography or NMR spectroscopy, including development of bioinformatic techniques and LIMS tools. Using our protein production platform, we refine methods for improving the yield of structures from high-value targets, in particular proteins from humans and other vertebrates. CESG has a substantial outreach component; more than 400 targets from outside requestors have been accepted for study with a structure success rate of 5%, which compares favorably with the eukaryotic success rates for the total PSI effort. These eukaryotic targets frequently present unique challenges. All CESG protein production protocols and Technology Dissemination Reports are accessible through the PSI Knowledgebase: http://kb-psi-structuralgenomics.org/KB/ and CESG's website. Selected technology developments are presented here. These include advances in expression vector design, enhanced methodology for cell based and wheat germ cell-free expression systems, new software to improve the quality and reduce time for structure determination by X-ray crystallography and NMR, and optimized techniques for the production of TEV protease for use in our protein production platform. We actively share our advances with the biotechnology, pharmaceutical, and academic communities through collaborations, oral presentations, peer-reviewed articles, submissions to public databases and material distribution channels, including PepcDB, PDB, BMRB, PSI Materials Repository, and technology transfer workshops. Supported by NIH/NIGMS Protein Structure Initiative grants GM074901 and GM064598. We acknowledge ALL members of the CESG team for their dedicated work.

Fruit ripening in Vitis vinifera: apoplastic solute accumulation accounts for pre-veraison turgor loss in berries

In Vitis vinifera L. berries, the onset of ripening (known as "veraison") involves loss of turgor (P) in the mesocarp cells. We hypothesized that P loss was associated with an accumulation of apoplastic solutes in mesocarp tissue prior to veraison. Apoplastic sap was extracted from the mesocarp by centrifugation at the appropriate gravity to measure the apoplast solute potential (Psi (s) (A) ) and assay the sap composition. The Psi (s) (A) was about -0.2 MPa early in development, decreased about 1.0 MPa by veraison, and continued to decrease during ripening to almost -4.0 MPa by the end of berry development. Potassium, malate, tartrate, proline, glucose, fructose, and sucrose were quantified in apoplastic sap. The calculated contribution of these solutes was about 50% of the total Psi (s) (A) preveraison, but increased to about 75% as fructose and glucose accumulated during ripening. The contribution of the estimated matric potential to apoplast water potential decreased during development and was only 1.5% postveraison. We conclude that high concentrations of solutes accumulated in the mesocarp apoplast prior to veraison, and that P loss was a direct result of decreased Psi (s) (A) . Because Psi (s) (A) decreased before veraison, our findings suggest that apoplast solutes play an important role in the events of cellular metabolism that lead to the onset of ripening.

Drillinge im Alter von 3 Jahren - Entwicklungsstand der Kinder und Zufriedenheit der Eltern mit dem Familienleben

Fragestellung: Vergleich von Drillingen und deren Familien mit ehemals reif geborenen Einlingskindern und ihren Familien, um anhand dieser Daten eine Verbesserung der präpartalen Beratung zu erreichen.

Analysis of leaf water relations in leaves of two olive (Olea europaea) cultivars differing in tolerance to salinity.

One-year-old rooted cuttings of olive (Olea europaea L. cvs. Frantoio and Leccino) were grown either hydroponically or in soil in a greenhouse. Plants were exposed to NaCl treatments (0, 100, and 200 mM) for 35 days, followed by 30 to 34 days of relief from salt stress to determine whether previously demonstrated genotypic differences in tolerance to salinity were related to water relations parameters. Exposure to high salt concentrations resulted in reductions in predawn water potential (Psi(w)), osmotic potential at full turgor (Psi(piFT)), osmotic potential at turgor loss point (Psi(piTLP)), and relative water content (RWC) in both cultivars, regardless of the growth substrate. Leaf Psi(w) and RWC returned to values similar to those of controls by the end of the relief period. The effect of salinity on Psi(pi) appeared earlier in Leccino than in Frantoio. Values for Psi(piFT) were -2.50, -2.87, and -3.16 MPa for the 0, 100, and 200 mM salt-treated Frantoio plants, respectively, and -2.23, -2.87, and -3.37 MPa for the corresponding Leccino plants. Recovery of Psi(pi) was complete for plants in the 100 mM salt treatment, but not for plants in the 200 mM salt treatment, which maintained an increased pressure potential (Psi(pi)) compared to control plants. Net solute accumulation was higher in Leccino, the salt-sensitive cultivar, than in Frantoio. In controls of both cultivars, cations contributed 39.9 to 42.0% of the total Psi(piFT), mannitol and glucose contributed 27.1 to 30.8%, and other soluble carbohydrates contributed 3.1 to 3.6%. The osmotic contribution of Na(+) increased from 0.1-2.1% for non-treated plants to 8.6-15.5% and 15.6-20.0% for the 100 mM and 200 mM salt-treated plants, respectively. The mannitol contribution to Psi(piFT) reached a maximum of 9.1% at the end of the salinization period. We conclude that differences between the two cultivars in leaf water relations reflect differences in the exclusion capacities for Na(+) and Cl(-) ions.