AbstractThe essential oil composition of the aerial parts of Anemia tomentosa var. anthriscifolia (Anemiaceae) was characterized by GC–MS. Total volatile oil yield extracted by steam distillation was 0.2% dry weight basis. The volatile oil was composed mainly of sesquiterpenes (75%, as the relative percentage of the total essential oil) with lower amounts of monoterpenes (15%); no phenylpropanoids were detected. The sesquiterpenes were composed mainly of oxygenated components (67%), including α‐bisabolol (51%), spathulenol (1%), caryophyllene oxide (3%), α‐bisaboloxide (1%) and 14‐hydroxy‐9‐epi‐(E)‐caryophyllene (1%). The monoterpenes were dominated by neral (5%) and geranial (7%), with lower amounts of α‐pinene, camphene, 6‐methyl‐5‐hepten‐2‐one, 1,8‐cineole and pinocarveol. This is the first report of the essential oil accumulation (0.2%) and volatile composition in the aromatic fern A. tomentosa. Copyright © 2004 John Wiley & Sons, Ltd.