A preliminary disease survey in livestock and health management practices comprising cattle, goats, and poultry was undertaken. This study was conducted to identify livestock diseases, determine the method of diagnosis, and evaluate the disease management system within the period of 2020 to 2022. A semi-structural questionnaire was developed. 86 animal owners, who have large number of animals, participated in three districts Assosa, Bambasi, and Homosha each consisting of 52, 18, and 16 respondents respectively. The mean average number of cattle, goats, and poultry per farm was 10.1, 10.8, and 2003.3 in that order. According to respondents 93% of tell us the first disease was trypanosmiasis, followed by parasites 70.9%. Newcastle disease 75.6% of respondents was the most common disease in poultry, and Peste des petites ruminants (46.5% of respondents) was the most common in small ruminants. Most respondents explained that there was low satisfaction in veterinary supply. In Homosha, Assosa and Bambasi district identification of diseases diagnosis only by clinical sign was 100%, 80.7% and 33.3% respectively. Season of outbreak vary according to diseases type and infected species. The majority of goat and sheep outbreaks occurred in the winter (keremt) and autumn, but cattle outbreaks were prevalent in the spring followed by summer (bega). The total morbidity was 16.8% and total mortality was 7.1%. In Bambasi, the average morbidity was 2.8% and average mortality was 0.1%. The morbidity in Homosha was 62.5% and mortality was 26.1%. In Assosa districts the morbidity was 7.6% and mortality 3.7%. The morbidity of cattle was 13.2% and mortality was 5.5%. The morbidity of goat was 51.7% and mortality was 23.3 %. The morbidity in poultry farms was 26.8% and mortality 11.2%. This Study revealed that there were different disease outbreaks in the Assosa zone and management in livestock Production is poorly practiced. This is the greatest threat to livestock production and productivity. Therefore, preventive measures should be taken to sustain livestock health.