Total Mixed Ration Particle Size Research Articles

Ration particle size has different effects on digestive but not production parameters in higher-yielding (Holstein) compared to lower-yielding (Girolando) cows.

The aim of the study was to evaluate the effect of total mixed ration particle size (length) and breed of cow on intake dynamics, animal performance and CH4 emissions, comparing high yielding Holstein and low yielding Girolando cows. The experimental design was 2 × 2 Latin Square arranged as a crossover factorial scheme with two diets (short particle size, SPS and long particle size, LPS) and the two breed compositions. The design comprised two periods of 26 d each, where all data collection was performed at cow level. No influence of the particle size occurred for the passage rate, neutral detergent fiber digestibility, performance and milk composition, methane emissions or ruminal fermentation parameters. Girolando cows had greater dry matter intake (DMI) when fed SPS, while Holsteins had the same (P < 0.05). Girolando cows had lower dry matter digestibility when fed LPS compared to SPS, while Holsteins had the opposite effect (P < 0.05). Also, the digestibility of crude protein and non-fibrous carbohydrates decreased in Girolando cows fed LPS, but not in Holsteins (P < 0.05). Girolando cows reduced DMI by 10.6% when fed LPS diet (P < 0.05). Girolando had an increased eating rate (+24 g of DM/min; P < 0.05) compared to Holstein cows, but Holstein cows had a lower CH4 intensity (by 29.7%: P < 0.05). Girolando cows increased the dry matter intake when fed a diet with short particle size, while the same did not happen in Holsteins. Dry matter digestibility increased in Holsteins when fed long particle size, while the opposite was observed in Girolando cows. Nutrient digestibility was reduced in Girolando cows when fed short particle size. Particle size did not influence eating time, eating rate, feed trough visits, visits with intake, milk yield and composition regardless of the breed. Reducing particle size increased CH4 intensity in both breeds.

Case Study: Farm characteristics and total mixed ration particle size issues on Parmigiano Reggiano farms in northern Italy

ABSTRACT A convenience sample of 20 farms in the geographic bounds of the Parmigiano Reggiano cheese producing region of northern Italy were studied. All farms used a TMR feeding system consisting of all dry hay (primarily alfalfa and grass) diets and adhering to the specific limitations of feeds as described by the Parmigiano Reggiano cheese consortium. Farms were visited twice for a survey of general farm characteristics and feeding management practices and collection of feed intake and samples at 0, 8, and 24 h after feeding to measure particle size characteristics of TMR in the feed bunk at each time. Many farms added 1 to 10 L of water per cow to the TMR in an attempt to reduce sorting and enhance intake. Based on particle size analysis it appeared that cows were not sorting diets to any great degree either with or without added water. However, it is likely that adding water to the TMR enabled cows to eat faster, thereby producing less saliva per kilogram of feed, and helped to create a situation for more slug feeding in the warm summer months of this study when the water-added TMR heated due to molding several hours after feeding. There was a positive correlation (r = 0.41) between the amount of added water and the percentage of cows on a farm that had an inversion of milk fat and protein or low fat percentage. Farms using pelleted concentrates also had less milk fat (r = −0.41). Overall there appeared to be no advantage of adding water to reduce TMR sorting on these farms.

The Association between Total Mixed Ration Particle Size and Fecal Scores in Holstein Lactating Dairy Cows from Florida, USA

The objective of this study was to determine the association between the proportion of particles greater than 19 mm in a total mixed ration fed to dairy cattle and their fecal score. Over a 22 day period, random feed samples were collected twice a day from a one-side barn and assessed for particle size using the Pennsylvania State Particle Separator. In addition, at each feeding sampling, the same barn was inspected and fresh feces were scored on a four point scale. Multiple regression models were developed to associate the proportion of particles >19 mm with fecal scores at one, two and three days after that specific particle evaluation. Results of this observational study showed a significant relationship between particle size and fecal scores two days later. The results demonstrated a cubic polynomial effect: Fecal scores improved with increasing of the proportion of particle size >19 mm at a low proportion of large particles, then it was maintained constant at intermediate proportion (10-15%) of large particles and finally increased again with a high proportion (up to 17%) of particle >19 mm.

Feeding lactating dairy cattle long hay separate from the total mixed ration can maintain dry matter intake during incidents of low rumen pH

The objective of this study was to investigate effects of offering dry hay of different quality and length on rumen pH and feed preference in lactating dairy cows. Eight rumen-cannulated Holstein cows (104±34 d in milk, body weight of 601±116kg, and parity of 2.38±1.69; mean ± standard deviation) were used in a replicated 4×4 Latin square design. Each period encompassed 21 d divided into 5 phases: adaptation (d 1 to 14), with ad libitum total mixed ration (TMR); baseline (d 15 to 17), with ad libitum TMR; restricted feeding (d 18), with cows fed for 75% of baseline dry matter intake; challenge (d 19), with 4kg (as-fed) of finely ground wheat mixed into the digesta of each cow via rumen cannula before feeding; and recovery (d 20 to 21), with ad libitum TMR. Cows were assigned to squares by parity and randomly assigned to treatments. Treatments were 5.2% low-quality hay TMR (CL), 5.2% high-quality hay TMR (CH; both hays were chopped and included in TMR), TMR with 5.2% supplemental long low-quality hay (TMR+L), and TMR with 5.2% supplemental long high-quality hay (TMR+H; both hays were unprocessed and fed separate from TMR).Low-quality hay contained 8.6% crude protein and 67.1% neutral detergent fiber, whereas high-quality hay contained 14.4% crude protein and 56.2% neutral detergent fiber. Animals were housed individually, milked twice per day, and fed once per day for 10% refusal rate. Data were analyzed using PROC MIXED of SAS (SAS Institute Inc., Cary, NC). Subacute ruminal acidosis challenge decreased weighted average rumen pH from 5.72 to 5.51. Cows fed TMR+L had higher rumen pH compared with CL and TMR+H on d 19. During d 20, cows fed chopped hay had higher rumen pH than cows fed supplemental long hay. Cows fed supplemental long hay had greater dry matter intake during baseline and challenge days compared with when hay was chopped and included in the TMR. Minimal differences among diets were found for TMR particle size selection during the challenge day; however, cows had a greater preference for high-quality long hay during recovery days. Milk production averaged 38.3kg/d and did not differ among treatments. Fat, protein, and lactose yields were also not different among treatments. Milk fatty acid profile was altered by treatment. The TMR+L and CH treatments increased production of cis-9,trans-11 conjugated linoleic acid. Results of this study indicate that feeding TMR plus supplemental long hay can maintain dry matter intake during incidents of and recovery from periods of low ruminal pH.

Feeding behaviors of transition dairy cows fed glycerol as a replacement for corn

Feed sorting is a natural behavior of dairy cows that can result in inconsistencies in the nutritive value of a total mixed ration (TMR). The objective of this study was to determine the effects of replacing high-moisture corn with glycerol on feed sorting and the feed intake pattern of transition dairy cows. Multiparous Holstein cows (n=26) were paired by expected calving date, housed in individual tie stalls, and fed diets containing either glycerol or high-moisture corn once daily from d −28 to +56 relative to calving. Glycerol was included at 11.5 and 10.8% of the ration dry matter for the pre- and postpartum diets, respectively. The feed consumption pattern was determined by measuring TMR disappearance during the intervals from 0 to 4h, 4 to 8h, 8 to 12h, and 12 to 24h relative to feed delivery. Feed sorting was determined on d −16, −9, 9, 16, and 51 relative to calving at 4, 8, 12 and 24h after feeding. The TMR particle size profile was determined at feed delivery and at 4, 8, 12, and 24 after feed delivery by using the Penn State Particle Separator (Nasco, Fort Atkinson, WI) to yield long (>19mm), medium (<19mm, >8mm), short (<8mm, >1.18mm), and fine (<1.18mm) particles. Overall feed intake did not differ between diets and was 14.7±0.4 and 20.2±0.5 kg/d for the pre- and postpartum intervals, respectively. During the prepartum period, glycerol decreased the amount of feed consumed during the first 4h after feed delivery (7.22 vs. 5.59±0.35 kg; control vs. glycerol, respectively) but increased feed consumed from 12 through 24h after feed delivery (2.22 vs. 3.82±0.35 kg; control vs. glycerol, respectively). Similar effects on the feed consumption pattern were observed after calving. During the prepartum period, cows fed the control diet sorted against long particles, whereas cows fed glycerol did not sort against long particles (77.2 vs. 101.5±3.50% of expected intake for control vs. glycerol; significant treatment effect). The data indicate that addition of glycerol to the TMR alters the feed consumption pattern to increase feed consumption late in the day at the expense of feed consumed immediately after feeding, and it reduces sorting behavior against long particles. Together, these may reduce diurnal variations in the rumen environment to promote greater rumen health in transition cows.

Effect of varying total mixed ration particle size on rumen digesta and fecal particle size and digestibility in lactating dairy cows

The objective of this experiment was to evaluate the effects of feeding rations of different particle sizes on rumen digesta and fecal matter particle size. Four rumen-cannulated, multiparous, Holstein cows (104±15 d in milk) were randomly assigned to treatments in a 4×4 Latin square design. The diets consisted of 29.4% corn silage, 22.9% ground corn, 17.6% alfalfa haylage, and 11.8% dry grass hay [20% of forage dry matter (DM)] on a DM basis. Dry grass hay was chopped to 4 different lengths to vary the total mixed ration (TMR) particle size. Geometric mean particle sizes of the rations were 4.46, 5.10, 5.32, and 5.84mm for short, medium, long, and extra long diets, respectively. The ration affected rumen digesta particle size for particles ≥3.35mm, and had no effect on distribution of particles <3.35mm. All rumen digesta particle size fractions varied by time after feeding, with soluble particle fractions increasing immediately after feeding and 0.15, 0.6, and 1.18-mm particle size fractions decreasing slightly after feeding. Particle fractions >1.18mmhad ration by time interactions. Fecal neutral detergent fiber (NDF) and indigestible NDF concentrations decreased with increasing TMR particle size. Fecal particle size expressed as total geometric mean particle length followed this same tendency. Fecal particle size, expressed as retained geometric mean particle length, averaged 1.13mm with more than 36% of particles being larger than 1.18mm. All fecal nutrient concentrations measured were significantly affected by time after feeding, with NDF and indigestible NDF increasing after feeding and peaking at about 12h later and then decreasing to preprandial levels. Starch concentrations were determined to have the opposite effect. Additionally, apparent digestibility of diet nutrients was analyzed and DM digestibility tended to decrease with increasing TMR particle size, whereas other nutrient digestibilities were not different among rations. These results show that the critical size for increased resistance to rumen escape is larger than 1.18mm and this critical size is constant throughout the day. This study also concludes that, when using average quality grass hay to provide the range of particle sizes fed, DM digestibility tends to decrease with increasing ration particle size.

Effect of feed sorting on chewing behavior, production, and rumen fermentation in lactating dairy cows

Ration sorting is thought to allow cows to eat different rations throughout the day, causing fluctuations in rumen fermentation patterns that can be detrimental to production and possibly animal health. The objective of this experiment was to study the effects of varying total mixed ration (TMR) particle size on sorting behavior of lactating dairy cows and to evaluate effects on chewing behavior, milk yield, milk components, and rumen fermentation. Eight multiparous, Holstein cows (90±32 d in milk; 4 rumen cannulated) were randomly assigned to replicated 4×4 Latin squares. Cows were fed diets that varied in the chop length of dry grass hay. The diet consisted of 29.4% corn silage, 22.9% ground corn, 17.6% alfalfa haylage, and 11.8% dry grass hay on a dry matter basis. The percentage of hay particles >26.9mm was 4.2, 34.1, 60.4, and 77.6% for the short (S), medium (M), long (L), and extra long (XL) hays, respectively. This resulted in the TMR of each diet having 1.5 (S), 6.5 (M), 8.6 (L), and 11.7% (XL) of particles >26.9mm. Daily ruminating time [19.3, 19.2, 22.4, and 21.3 min/kg of dry matter intake (DMI) for S, M, L, and XL] and eating time (13.9, 14.6, 17.2, and 16.1 min/kg of DMI for S, M, L, and XL) increased linearly as TMR particle size increased. Daily DMI decreased linearly as TMR particle size increased and was 26.9 (S), 27.0 (M), 24.1 (L), and 25.1 (XL) kg/d. No differences were found in rumen volatile fatty acids and NH3, and only slight changes were found in rumen pH. Milk production and milk components were also similar among diets. Despite large differences in particle size among these diets and certain chewing and ruminating differences, no changes in rumen fermentation, milk production, or milk components were found in this study.

Technical note: Evaluation of procedures for analyzing ration sorting and rumen digesta particle size in dairy cows

Collecting total mixed ration (TMR) samples throughout the day to measure sorting activity of dairy cows may cause changes to the sorting behavior of cows or may make it more difficult to elucidate effects of sorting on TMR particle size distributions. Also, forage particle size research commonly includes analysis of the solid portion of rumen digesta for particle size distribution after digesta has been squeezed through several layers of cheesecloth. Therefore, the first objective of this experiment was to determine if collecting TMR samples throughout the day affected sorting behavior of cows and resulted in a different particle size distribution than when TMR was not artificially altered during the day. The second objective of this experiment was to determine if squeezing rumen digesta samples through cheesecloth changed particle size distribution when analyzed by a wet sieving technique. It was determined that small, significant differences existed in particle size distribution between the 2 sampling methods of TMR for sorting behavior. These differences were more likely to occur at time points later in the day. This resulted in small changes in sorting indices calculated from these data; sampling and mixing TMR throughout the day reduced the degree of sorting. Squeezing rumen digesta through 4 layers of cheesecloth had no effect on particle size distribution of particles >0.15mm but reduced the amount of rumen fluid-associated dry matter contained in the sample.

Use of maize silage in beef cattle feeding during the finishing period

The research investigated the use of maize silage (MS) in beef cattle diets during the finishing period by monitoring a sample of 406 commercial farms located in the Po Valley. Farms were selected in order to cover the most diverse rearing situations, in terms of farm size and cattle genotype, in which MS was fed to beef cattle during the finishing period. Each farm was visited to collect information about the feeding regimen and representative samples of total mixed ration (TMR) and MS were collected for chemical and physical analysis. Two specific classification factors of the farm population were created for statistical analysis of experimental data. The former considered the quantity of MS included in the TMR and the latter was based on the content of long particles of MS (>13 mm). Regardless of chop length, the quality of MS population was satisfactory both by a nutritional and a preservation standpoint, as indicated by the dry matter (DM) (350 ± 38 g/kg of fresh weight) and the high starch content (310 ± 28 g/kg DM) and lactic acid concentration (49 ± 12 g/kg DM). Physical analysis of MS samples showed a wide range of particle size, as result of the different chopping lengths during harvest. The average content of long particles retained by a 13-mm screen was 143 ± 73 g/kg of fresh weight. On average, MS accounted for 33.4% to the total dietary DM but a large standard deviation (11.4%) was observed across farms. The decision about the amount of MS to be included in the TMR has shown to be independent on both chemical composition and particle size of the roughage. In diets with a high quantity of MS the risk of occurrence of rumen acidosis due to the additional starch brought by this silage is balanced either by a significant reduction in the amount of other starch sources or by the higher NDF content and the coarser size of the dietary particles which should promote a prolonged rumination. The analysis of TMR particle size showed that in more than 30% of the farms MS was damaged during diet preparation with a loss of long particles. A logistic analysis demonstrated that the relative risk of MS damage was significantly increased either by a larger inclusion of the roughage in the TMR or by the use of silage with coarse particles.

Particle size evaluation of total mixed rations in intensive beef production systems

Samples of total mixed ration (TMR) were collected periodically in 15 beef cattle farms and submitted to a nutritional and particle size evaluation. Samples of faeces were taken and analysed for pH, consistency, colour and visually ranked for corn residues (Corn Residue Index) from 4 (totally digested) to 0 (very high presence of maize residues). Dry matter (DM), crude protein (CP), and ash content of TMR for Limousine were higher than that for Charolaise while an opposite situation was observed for crude fiber (CF). Particle size analysis of TMR showed an higher content of small (<1.8 mm) and large (>19.0 mm) particles in Charolaise than in Limousine TMR. From May to September a general increase of larger fractions and a reduction of the smaller ones were observed, as a consequence of empirical practices of TMR formulation during summer. Faeces characteristics did not show any difference between breeds (overall mean pH: 6.00 vs 6.13; Corn Residue Index: 2.90 vs 3.24, respectively for Charolaise and Limousine). A minimum value for pH was observed in November. Corn Residues Index decreased from May to September and than increased reaching values comparable with those observed at the beginning of the trial. These data would indicate a worsening of digestibility for non-fibrous carbohydrates, leading to an increase in the excretion of undigested corn and an increase of starch intestinal fermentation, in particular during summer. Such observations suggest to better standardize the feeding strategy in intensive beef production systems in order to optimize digestibility and productive performances. Further investigations are necessary in order to assess an optimal large/small particle size ratio of TRMs. Our findings highlight the usefulness of TMR particle size evaluation and visual/chemical analysis of faeces as a simple diagnostic methods also for beef cattle production management.

Effect of Total Mixed Ration Particle Size on Rumen pH, Chewing Activity, and Performance in Dairy Cows

Two experiments were conducted to determine effects of panicle size in total mixed ration (TMR) on performance of lactating cows. Three rumen cannulated Holstein cows were used in a 3×3 Latin square design for the metabolic experiment. The particle size of the diets was determined using the Penn State Particle Size Separator (PSPSS) and weighing the proportion of sample remaining on the top screen (19 mm diameter). The 3 treatments were short, medium or long diets (4.9, 24.2 and 27.8% of sample remaining on the top screen of the PSPSS, respectively). Nine farms in the Edmonton area were surveyed and the farms were placed into groups based on the particle size of the ration fed. The groups were short ≤6%, medium 7-12% and long ≥13% of sample weight remaining on the top screen of the PSPSS. Dry matter intake was greater (p=0.07) for the medium diet than the long diet in the metabolic study and resulted in a higher (p=0.07) efficiency of milk production. On the commercial farms, a significantly (p=0.002) lower milk fat percentage was observed for the long diet compared to the short diet. The results of these studies confirm that forage particle size influences milk composition and milk fat was negatively correlated to TMR particle size.

Effect of Forage and TMR Particle Size on Cow Health and Milk Components

Particle size of forages and total mixed rations plays an important role in the health and performance of dairy cattle. Objective of this study was to determine the effect of forage and total mixed ration (TMR) particle size on various health and production parameters in Ontario dairy herds.

Processing, mixing, and particle size reduction of forages for dairy cattle.

Adequate forage amounts in both physical and chemical forms are necessary for proper ruminal function in dairy cows. Under conditions in which total amounts of forage or particle size of the forage are reduced, cows spend less time ruminating and have a decreased amount of buoyant digesta in the rumen. These factors reduce saliva production and allow ruminal pH to fall, depressing activity of cellulolytic bacteria and causing a prolonged period of low ruminal pH. Insufficient particle size of the diet decreases the ruminal acetate-to-propionate ratio and reduces ruminal pH. The mean particle size of the diet, the variation in particle size, and the amount of chemical fiber (i.e., NDF or ADF) are all nutritionally important for dairy cows. Defining amounts and physical characteristics of fiber is important in balancing dairy cattle diets. Because particle size plays such an important role in digestion and animal performance, it must be an important consideration from harvest through feeding. Forages should not be reduced in particle size beyond what is necessary to achieve minimal storage losses and what can be accommodated by existing equipment. Forage and total mixed ration (TMR) particle sizes are potentially reduced in size by all phases of harvesting, storing, taking out of storage, mixing, and delivery of feed to the dairy cow. Mixing feed causes a reduction in size of all feed particles and is directly related to TMR mixing time; field studies show that the longest particles (>27 mm) may be reduced in size by 50%. Forage and TMR particle size as fed to the cows should be periodically monitored to maintain adequate nutrition for the dairy cow.