The volume of glaciers in Iceland (∼3,400 km3in 2019) corresponds to about 9 mm of potential global sea level rise. In this study, observations from 98.7% of glacier covered areas in Iceland (in 2019) are used to construct a record of mass change of Icelandic glaciers since the end of the 19th century i.e. the end of the Little Ice Age (LIA) in Iceland. Glaciological (in situ) mass-balance measurements have been conducted on Vatnajökull, Langjökull, and Hofsjökull since the glaciological years 1991/92, 1996/97, and 1987/88, respectively. Geodetic mass balance for multiple glaciers and many periods has been estimated from reconstructed surface maps, published maps, aerial photographs, declassified spy satellite images, modern satellite stereo imagery, and airborne lidar. To estimate the maximum glacier volume at the end of the LIA, a volume–area scaling method is used based on the observed area and volume from the three largest ice caps (over 90% of total ice mass) at 5–7 different times each, in total 19 points. The combined record shows a total mass change of −540 ± 130 Gt (−4.2 ± 1.0 Gt a−1on average) during the study period (1890/91 to 2018/19). This mass loss corresponds to 1.50 ± 0.36 mm sea level equivalent or 16 ± 4% of mass stored in Icelandic glaciers around 1890. Almost half of the total mass change occurred in 1994/95 to 2018/19, or −240 ± 20 Gt (−9.6 ± 0.8 Gt a−1on average), with most rapid loss in 1994/95 to 2009/10 (mass change rate −11.6 ± 0.8 Gt a−1). During the relatively warm period 1930/31–1949/50, mass loss rates were probably close to those observed since 1994, and in the colder period 1980/81–1993/94, the glaciers gained mass at a rate of 1.5 ± 1.0 Gt a−1. For other periods of this study, the glaciers were either close to equilibrium or experienced mild loss rates. For the periods of AR6 IPCC, the mass change rates are −3.1 ± 1.1 Gt a−1for 1900/01–1989/90, −4.3 ± 1.0 Gt a−1for 1970/71–2017/18, −8.3 ± 0.8 Gt a−1for 1992/93–2017/18, and −7.6 ± 0.8 Gt a−1for 2005/06–2017/18.