Marination is an important part of meat processing, the disadvantage is that the process of marinating is slow and prone to losing nutrients. In this study, pork tenderloins were pretreated with pulsed electric field (PEF) before being immersed in the marinade at 4 °C. The aim was to investigate the effect of PEF pretreatment (PEFP) on the marination process, specifically mass transfer and the quality of the pork. Additionally, the response surface method (RSM) was employed to explore the optimal conditions for PEFP. Results showed that under the best condition (2 kV/cm, 141.2 Hz and 2.3% duty cycle), the NaCl content of pork cured for 6 h reached 3.31%, which could effectively shorten the marinating time of pork (up to 40%). After marinating for 6 h with PEFP, the weight, moisture, and salt changes increased significantly. The effective diffusion coefficient (De) was raised to 1.71 × 10−10 (PEFP) from 1.36 × 10−10 (control) and the kinetic model of mass transfer over time has good linear correlation. The scanning electron microscopy (SEM) results indicate that PEFP altered the muscle fibre structure of pork to some extent, resulting in loosely arranged muscle fibre bundles. The total bacterial colony count results showed that PEFP had a good sterilization effect on microorganisms in pork. Furthermore, PEFP does not alter the colour or pH of marinated pork while improving its tenderness. These results showed that PEFP could effectively promote salt diffusion to shorten marinating time and improve meat tenderness.