Tomato Root Exudates Research Articles

Tomato Root Exudates Infected by Meloidogyne incognita Impact the Colonization of Nematicidal Proteus vulgaris

Root exudates play a pivotal role in shaping the microbial community in the rhizosphere and can impact the efficacy of bacteria in controlling nematode populations. This study identified Proteus vulgaris BX-1 as significantly effective in controlling Meloidogyne incognita. The infection of tomato plants with this nematode induced noticeable alterations in the composition of tomato root exudates and led to an increased colonization rate of strain BX-1. Further investigation into how strain BX-1 responded to changes in tomato root exudates revealed that specific metabolites, such as caffeic acid, coumarin, salicylic acid, sebacic acid, and butyric acid, strongly attracted strain BX-1. This attraction potentially contributed to its enhanced colonization and improved efficiency in controlling nematodes. Understanding the correlation between specific metabolites in root exudates and the response of antagonistic bacteria provides valuable insights for enhancing their effectiveness as biological control agents against plant-parasitic nematodes.

Unraveling the Roles of Neuropeptides in the Chemosensation of the Root-Knot Nematode Meloidogyne javanica.

The identification of novel drug targets in plant-parasitic nematodes (PPNs) is imperative due to the loss of traditional nematicides and a lack of replacements. Chemosensation, which is pivotal for PPNs in locating host roots, has become a focus in nematode behavioral research. However, its underlying molecular basis is still indistinct in such a diverse group of PPNs. To characterize genes participating in chemosensation in the Javanese root-knot nematode Meloidogyne javanica, RNA-sequencing of the second-stage juveniles (J2s) treated with tomato root exudate (TRE) for 1 h and 6 h was performed. Genes related to chemosensation in M. javanica mainly responded to TRE treatment at 1 h. Moreover, a gene ontology (GO) analysis underscored the significance of the neuropeptide G protein-coupled receptor signaling pathway. Consequently, the repertoire of putative neuropeptides in M. javanica, including FMRFamide-like peptides (FLPs), insulin-like peptides (ILPs), and neuropeptide-like peptides (NLPs), were outlined based on a homology analysis. The gene Mjflp-14a, harboring two neuropeptides, was significantly up-regulated at 1 h TRE treatment. Through peptide synthesis and J2 treatment, one of the two neuropeptides (MjFLP-14-2) was proven to influence the J2 chemotaxis towards tomato root tips. Overall, our study reinforces the potential of nematode neuropeptides as novel targets and tools for root-knot nematode control.

Effect of n-hexadecanoic acid on N2O emissions from vegetable soil and its synergism with Pseudomonas stutzeri NRCB010

Recently, it has been recommended to utilize specific plant root metabolites to decrease nitrous oxide (N2O) emissions from agricultural soil. However, only a limited number of plant root metabolites have been shown to decrease soil N2O emissions, and their combined effects with plant growth-promoting rhizobacteria, as well as the mechanisms involved in mitigating N2O emissions, remain to be explored. This study investigated the impact and microbial mechanisms of three tomato root exudate metabolites, namely, methyl hexadecanoate, methyl stearate, and n-hexadecanoic acid, on N2O emissions. In a pure culture experiment, n-hexadecanoic acid significantly enhanced Pseudomonas stutzeri NRCB010 auxin production, nitrogen removal, N2O reduction capacity, and N2O reductase activity. Methyl stearate significantly promoted NRCB010 N removal and the N2O reduction capacity. In soil microcosm and greenhouse pot experiments, n-hexadecanoic acid decreased N2O cumulative emissions by 34.3 % and 46.6 %, respectively, and further decreases were observed when combined with NRCB010. Additionally, n-hexadecanoic acid, both independently and in combination with NRCB010, significantly promoted tomato growth. N-hexadecanoic acid, alone or with NRCB010, also altered soil microbial communities and nitrogen-cycle functional gene abundance. Structural equation models revealed that n-hexadecanoic acid, either alone or with NRCB010, decreased N2O cumulative emissions by modifying the environmental conditions to be more conducive to N2O mitigation (e.g., higher soil pH and organic matter content), inhibiting N2O production (through a decrease in ammonia-oxidizing bacteria and nirK gene copy numbers), and promoting N2O reduction (by increasing nosZII gene copy numbers). Our results support the potential use of n-hexadecanoic acid in enhancing crop productivity and mitigating N2O emissions in agricultural soils.

Utilization of plant-derived sugars and lipids are coupled during colonization of rhizoplane and rhizosphere by the fungus Metarhizium robertsii

Plant-derived sugars and lipids are key nutritional sources for plant associated fungi. However, the relationship between utilization of host-derived sugars and lipids during development of the symbiotic association remains unknown. Here we show that the fungus Metarhizium robertsii also needs plant-derived lipids to develop symbiotic relationship with plants. The fatty acid binding proteins FABP1 and FABP2 are important for utilization of plant-derived lipids as the deletion of Fabp1 and Fabp2 significantly reduced the ability of M. robertsii to colonize rhizoplane and rhizosphere of maize and Arabidopsis thaliana. Deleting Fabp1 and Fabp2 increased sugar utilization by upregulating six sugar transporters, and this explains why deleting the monosaccharide transporter gene Mst1, which plays an important role in utilization of plant-derived sugars, had no impact on the ability of the double-gene deletion mutant ΔFabp1::ΔFabp2 to colonize plant roots. FABP1 and FABP2 were also found in other plant-associated Metarhizium species, and they were highly expressed in the medium using the tomato root exudate as the sole carbon and nitrogen source, suggesting that they could be also important for these species to develop symbiotic relationship with plants. In conclusion, we discovered that utilization of plant-derived sugars and lipids are coupled during colonization of rhizoplane and rhizosphere by M. robertsii.

Characterization and performance evaluation of plant growth promoting bacteria in tomato rhizosphere

An acute and compelling need to replace chemical fertilizers has shifted our focus towards plant growth-promoting rhizobacteria (PGPR) that rapidly colonize the rhizosphere. In the present investigation, two isolates from tomato rhizosphere, TS4 and TS6, were screened for various PGPR properties. Both isolates expressed different properties such as enzyme production and hydrogen sulfide (H2S) production. Production of Indole-3-Acetic Acid (IAA) was 13.33 μg mL−1 for both TS4 and TS6. Ammonia excretion was 75.0 μg mL−1 for TS4 and 82.5 μg mL−1 for TS6. Both isolates tolerated salt stress up to 10% sodium chloride (NaCl), drought stress tolerated by isolate TS4 was up to -0.30 MPa and TS6 survived up to -0.49 MPa osmotic pressure. To assess the colonization potential of the isolates, their biofilm potential and chemotactic behavior towards the tomato root exudates were studied. Colonization of the tomato rhizosphere by TS4 and TS6 was observed quantitatively and found that root tips were more profusely colonized as compared to the root base. Nethouse studies to test their influence on tomato revealed a positive influence in some plant aspects. All the results led to the conclusion that isolates TS6 identified as Micrococcus luteus and TS4 belonging to genera Lactobacillus were efficient PGPR. This paper highlights the efficacy of PGPR as a component of sustainable agriculture for improved productivity. However, the need of risk assessment and rigorous surveillance of bacteria before developing them as a bioformulation is also emphasized.

Vanillin in Resistant Tomato Plant Root Exudate Suppresses Meloidogyne incognita Parasitism.

Tomato (Solanum lycopersicum) plants are susceptible to infection by root-knot nematodes, which cause severe economic losses. Planting resistant tomato plants can reduce nematode damage; however, the effects of resistant tomato root exudates in suppressing Meloidogyne incognita remain insufficiently understood. Here, we determined that the resistant tomato plant Lycopersicon esculentum cv. Xianke-8 (XK8) alleviates nematode damage by downregulating the expression of the essential parasitic nematode gene Mi-flp-18 to reduce the infection and reproduction of M. incognita. Using gas chromatography-mass spectrometry, we identified vanillin as a unique compound (compared to susceptible tomato cultivars) in XK8 root exudates that acts as a lethal trap and inhibitor of egg hatching. Moreover, the soil application of 0.4-4.0 mmol/kg vanillin significantly reduced galls and egg masses. The parasite gene Mi-flp-18 was downregulated upon treatment with vanillin, both in vitro and in pot experiments. Collectively, our results reveal an effective nematicidal compound that can use in feasible and economical strategies to control RKNs.

The biocontrol agent Bacillus velezensis T-5 changes the soil bacterial community composition by affecting the tomato root exudate profile

The biocontrol agent Bacillus velezensis T-5 changes the soil bacterial community composition by affecting the tomato root exudate profile

Certain Tomato Root Exudates Induced by Pseudomonas stutzeri NRCB010 Enhance Its Rhizosphere Colonization Capability

Plant growth-promoting rhizobacteria (PGPR) can colonize plant root surfaces or form biofilms to promote plant growth and enhance plant resistance to harsh external environments. However, plant-PGPR interactions, especially chemical signaling molecules, are poorly understood. This study aimed to gain an in-depth understanding of the rhizosphere interaction mechanisms between PGPR and tomato plants. This study found that inoculation with a certain concentration of Pseudomonas stutzeri significantly promoted tomato growth and induced significant changes in tomato root exudates. Furthermore, the root exudates significantly induced NRCB010 growth, swarming motility, and biofilm formation. In addition, the composition of the root exudates was analyzed, and four metabolites (methyl hexadecanoate, methyl stearate, 2,4-di-tert-butylphenol, and n-hexadecanoic acid) significantly related to the chemotaxis and biofilm formation of NRCB010 were screened. Further assessment showed that these metabolites positively affected the growth, swarming motility, chemotaxis, or biofilm formation of strain NRCB010. Among these, n-hexadecanoic acid induced the most remarkable growth, chemotactic response, biofilm formation, and rhizosphere colonization. This study will help develop effective PGPR-based bioformulations to improve PGPR colonization and crop yields.

Plant Genotype Shapes the Soil Nematode Community in the Rhizosphere of Tomatoes with Different Resistance to Meloidognye incognita

Soil nematodes are considered indicators of soil quality due to their immediate responses to changes in the soil environment and plants. However, little is known about the effects of plant genotypes on the soil nematode community. To elucidate this, high-throughput sequencing and gas chromatography/mass spectrometry analysis was conducted to analyze the soil nematode community and the structure of root exudates in the rhizosphere of tomatoes with different resistance to Meloidognye incognita. The dominant soil nematode group in the soil of resistant tomatoes was Acrobeloides, while the soil nematode group in the rhizosphere of the susceptible and tolerant tomatoes was Meloidognye. Hierarchical clustering analysis and non-metric multidimensional scaling showed that the three soil nematode communities were clustered into three groups according to the resistance level of the tomato cultivars. The soil nematode community of the resistant tomatoes had a higher maturity index and a low plant-parasite index, Wasilewska index and disease index compared to the values of the susceptible and tolerant tomatoes. Redundancy analysis revealed that the disease index and root exudates were strongly related to the soil nematode community of three tomato cultivars. Taken together, the resistance of the tomato cultivars and root exudates jointly shapes the soil nematode community. This study provided a valuable contribution to understanding the mechanism of plant genotypes shaping the soil nematode community.

Interspecific plant interaction via root exudates structures the disease suppressiveness of rhizosphere microbiomes

Terrestrial plants can affect the growth and health of adjacent plants via interspecific interaction. Here, we studied the mechanism by which plant root exudates affect the recruitment of the rhizosphere microbiome in adjacent plants—with implications for plant protection—using a tomato (Solanum lycopersicum)–potatoonion (Allium cepa var. agrogatum) intercropping system. First, we showed that the intercropping system results in a disease-suppressive rhizosphere microbiome that protects tomato plants against Verticillium wilt disease caused by the soilborne pathogen Verticillium dahliae. Second, 16S rRNA gene sequencing revealed that intercropping with potatoonion altered the composition of the tomato rhizosphere microbiome by promoting the colonization of specific Bacillus sp. This taxon was isolated and shown to inhibit V. dahliae growth and induce systemic resistance in tomato plants. Third, a belowground segregation experiment found that root exudates mediated the interspecific interaction between potatoonion and tomato. Moreover, experiments using split-root tomato plants found that root exudates from potatoonion, especially taxifolin—a flavonoid compound—stimulate tomato plants to recruit plant-beneficial bacteria, such as Bacillus sp. Lastly, ultra-high-pressure liquid chromatography–mass spectrometry analysis found that taxifolin alters tomato root exudate chemistry; thus, this compound acts indirectly in modulating root colonization by Bacillus sp. Our results revealed that this intercropping system can improve tomato plant fitness by changing rhizosphere microbiome recruitment via the use of signaling chemicals released by root exudates of potatoonion. This study revealed a novel mechanism by which interspecific plant interaction modulates the establishment of a disease-suppressive microbiome, thus opening up new avenues of research for precision plant microbiome manipulations.

Solanoeclepin B, a hatching factor for potato cyst nematode

The potato cyst nematode (PCN) causes extensive crop losses worldwide. Because the hatching of PCN requires host-derived molecules known as hatching factors (HFs), regulating HF production in host plants may help to control this harmful pest. Solanoeclepin A (SEA), isolated from potato, is the most active HF for PCN; however, its biosynthesis is completely unknown. We discovered a HF called solanoeclepin B (SEB) from potato and tomato root exudates and showed that SEB was biosynthesized in the plant and converted to SEA outside the plant by biotic agents. Moreover, we identified five SEB biosynthetic genes encoding three 2-oxoglutarate-dependent dioxygenases and two cytochrome P450 monooxygenases in tomato. Exudates from tomato hairy roots in which each of the genes was disrupted contained no SEB and had low hatch-stimulating activity for PCN. These findings will help to breed crops with a lower risk of PCN infection.

Identification of novel canonical strigolactones produced by tomato.

Canonical strigolactones (SLs), such as orobanchol, consist of a tricyclic lactone ring (ABC-ring) connected to a methylbutenolide (D-ring). Tomato plants have been reported to produce not only orobanchol but also various canonical SLs related to the orobanchol structure, including orobanchyl acetate, 7-hydroxyorobanchol isomers, 7-oxoorobanchol, and solanacol. In addition to these, structurally unidentified SL-like compounds known as didehydroorobanchol isomers (DDHs), whose molecular mass is 2 Da smaller than that of orobanchol, have been found. Although the SL biosynthetic pathway in tomato is partially characterized, structural elucidation of DDHs is required for a better understanding of the entire biosynthetic pathway. In this study, three novel canonical SLs with the same molecular mass as DDHs were identified in tomato root exudates. The first was 6,7-didehydroorobanchol, while the other two were not in the DDH category. These two SLs were designated phelipanchol and epiphelipanchol because they induced the germination of Phelipanche ramosa, a noxious root parasitic weed of tomato. We also proposed a putative biosynthetic pathway incorporating these novel SLs from orobanchol to solanacol.

Type-dependent effects of microplastics on tomato (Lycopersicon esculentum L.): Focus on root exudates and metabolic reprogramming

Much attention has been paid to the prevalence of microplastics (MPs) in terrestrial systems. MPs have been shown to affect the physio-biochemical properties of plants. Different MPs may have distinctive behaviors and diverse effects on plant growth. In the present study, the effects of polystyrene (PS), polyethylene (PE), and polypropylene (PP) MPs on physio-biochemical properties, root exudates, and metabolomics of tomato (Lycopersicon esculentum L.) under hydroponic conditions were investigated. Our results show that MPs exposure has adverse effects on tomato growth. MPs exposure had a significant type-dependent effect (p < 0.001) on photosynthetic gas parameters, chlorophyll content, and antioxidant enzyme activities. After exposure to MPs, the content of low molecular weight organic acids in tomato root exudates was significantly increased, which was considered as a strategy to alleviate the toxicity of MPs. In addition, MPs treatment significantly changed the metabolites of tomato root and leaf. Metabolic pathway analysis showed that MPs treatment had a great effect on amino acid metabolism. We also found that plants exposed to PS and PP MPs produced more significant metabolic reprogramming than those exposed to PE MPs. This study provides important implications for the mechanism studies on the toxic effect of various MPs on crops and their future risk assessment.

Biochar stimulates tomato roots to recruit a bacterial assemblage contributing to disease resistance against Fusarium wilt.

Biochar amendment is acknowledged to favor plant resistance against soil-borne diseases. Although plant-beneficial bacteria enrichment in the rhizosphere is often proposed to be associated with this protection, the mechanism behind this stimulating effect remains unelucidated. Here, we tested whether biochar promotes plants to recruit beneficial bacteria to the rhizosphere, and thus develop a disease-suppressive rhizosphere microbiome. In a pot experiment, biochar amendment decreased tomato Fusarium wilt disease severity. Using a transplanting rhizosphere microbiome experiment, we showed that biochar enhanced the suppressiveness of tomato rhizosphere microbiome against Fusarium wilt disease. High-throughput sequencing of 16Sribosomal RNA gene and in vitro cultures further indicated that the recruited suppressive rhizosphere microbiome was associated with the increase of plant-beneficial bacteria, such as Pseudomonas sp. This amendment also enhanced the in vitro chemoattraction and biofilm promotion activity of tomato root exudates. Collectively, our results demonstrate that biochar amendment induces tomato seedlings to efficiently recruit a disease-suppressive rhizosphere microbiome against Fusarium wilt.

The tomato cytochrome P450 CYP712G1 catalyses the double oxidation of orobanchol en route to the rhizosphere signalling strigolactone, solanacol.

Summary Strigolactones (SLs) are rhizosphere signalling molecules and phytohormones. The biosynthetic pathway of SLs in tomato has been partially elucidated, but the structural diversity in tomato SLs predicts that additional biosynthetic steps are required. Here, root RNA‐seq data and co‐expression analysis were used for SL biosynthetic gene discovery.This strategy resulted in a candidate gene list containing several cytochrome P450s. Heterologous expression in Nicotiana benthamiana and yeast showed that one of these, CYP712G1, can catalyse the double oxidation of orobanchol, resulting in the formation of three didehydro‐orobanchol (DDH) isomers.Virus‐induced gene silencing and heterologous expression in yeast showed that one of these DDH isomers is converted to solanacol, one of the most abundant SLs in tomato root exudate. Protein modelling and substrate docking analysis suggest that hydroxy‐orbanchol is the likely intermediate in the conversion from orobanchol to the DDH isomers.Phylogenetic analysis demonstrated the occurrence of CYP712G1 homologues in the Eudicots only, which fits with the reports on DDH isomers in that clade. Protein modelling and orobanchol docking of the putative tobacco CYP712G1 homologue suggest that it can convert orobanchol to similar DDH isomers as tomato.

Mixtures of suppressive bacteria enhance biological control of tomato bacterial wilt

Tomato bacterial wilt is one of the most destructive soil-borne plant diseases in the world, causing considerable economic losses. Biological control is an effective and environmentally friendly strategy to reduce disease incidence and yield loss. In this study, six Streptomyces-like strains, NEAU-Z2, NEAU-D18, NEAU-88, NEAU-174, NEAU-C12 and NEAU-66 exhibiting high antagonistic activities against Ralstonia solanacearum were isolated from tomato rhizosphere soil and used for further study. Of which strains NEAU-D18 and NEAU-88 were compatible with each other and the mixed strain (NEAU-D18 + NEAU-88) showing the strongest antibacterial activity against R. solanacearum were prepared. Hydroponic experiment at seedling stage showed that strains NEAU-D18 and NEAU-88 had the best control efficacy at the spore concentration of 108 cfu ml−1, which were 66.6% and 80%, respectively. Therefore, strains NEAU-D18 and NEAU-88 were inoculated into tomato seedling roots with different proportions (i.e., 1:1, 1:2, and 2:1) at the spore concentration of 108 cfu ml−1, and the ratio of 1:1 showed the best control efficacy (100%) on tomato bacterial wilt. Pot experiment also indicated that the mixed strain (NEAU-D18 + NEAU-88) had better biocontrol efficacy than that of single strains. Moreover, the mixed strain and R. solanacearum could utilize 31 of 51 tested compounds of tomato root exudates, indicating the mixed strain was more competitive than the single strains (27, 29) in theory. Furthermore, in vitro nutritional competition experiment showed that the incidence of tomato bacterial wilt with the addition of compounds that could not be utilized by R. solanacearum and mixed strain was equal to that of the control group (CK4-1), but the biocontrol efficacy was great different after adding six compounds that could be utilized by R. solanacearum and mixed strain, which confirmed that the nutritional competition occurred between the mixed strain and R. solanacearum. In addition, solid-state fermentation was used to prepare bioorganic fertilizer of mixed strain. The application of bioorganic fertilizer could effectively suppress the occurrence of tomato bacterial wilt, and the incidence was only 21.67 at 14 days post-challenge inoculation, while 85% of tomato plants developed bacterial wilt with organic fertilizer treatment. In conclusion, this study demonstrated that the mixed strain (NEAU-D18 + NEAU-88) showed better control efficacy than that of single strains and was a potential biocontrol agent for controlling tomato bacterial wilt.

UPLC-MS/MS analysis and biological activity of the potato cyst nematode hatching stimulant, solanoeclepin A, in the root exudate of Solanum spp.

Main conclusionSolanoeclepin A is a hatching stimulant for potato cyst nematode in very low (pM) concentrations. We report a highly sensitive method for the analysis of SolA in plant root exudates using UHPLC-MS/MS and show that there is considerable natural variation in SolA production in Solanum spp. corresponding with their hatching inducing activity.Potato cyst nematode (PCN) is a plant root sedentary endoparasite, specialized in the infection of solanaceous species such as potato (Solanum tuberosum) and tomato (Solanum lycopersicum). Earlier reports (Mulder et al. in Hatching agent for the potato cyst nematode, Patent application No. PCT/NL92/00126, 1996; Schenk et al. in Croat Chem Acta 72:593–606, 1999) showed that solanoeclepin A (SolA), a triterpenoid metabolite that was isolated from the root exudate of potato, induces the hatching of PCN. Its low concentration in potato root exudate has hindered progress in fully understanding its hatching inducing activity and exploitation in the control of PCN. To further investigate the role of SolA in hatching of PCN, the establishment of a highly sensitive analytical method is a prerequisite. Here we present the efficient single-step extraction and UHPLC-MS/MS based analysis for rapid determination of SolA in sub-nanomolar concentrations in tomato root exudate. This method was used to analyze SolA production in different tomato cultivars and related solanaceous species, including the trap crop Solanum sisymbriifolium. Hatching assays with PCN, Globodera pallida, with root exudates of tomato genotypes revealed a significant positive correlation between SolA concentration and hatching activity. Our results demonstrate that there is natural variation in SolA production within solanaceous species and that this has an effect on PCN hatching. The analytical method we have developed can potentially be used to support breeding for crop genotypes that induce less hatching and may therefore display reduced infection by PCN.

Root exudates increase phosphorus availability in the tomato/potato onion intercropping system

Crop diversification usually increase nutrient use efficiency and productivity of crops, but we still lack deeper understandings of the underlying mechanisms. Here we aimed to study the role of root exudates and soil microbial communities in mobilization of soil P during an intercropping system. Different barrier treatments were used to separate tomato and potato onion, and different P forms, that makes most of the plant sparingly available P in the soil (i.e., Al-P, Fe-P, Ca-P, Organic P), were applied in a pot experiment. In addition, we studied the effects of monoculture and/or intercropped tomato root exudates in vitro on soil available P and soil microbial community composition using quantitative PCR and high throughput sequencing. Compared with solid barrier, the 30 μm barrier increased belowground tomato biomass in presence of Ca-P, O-P and no P, soil available P in presence of Al-P, Fe-P and Ca-P, increased abundance of Bacillus spp. in presence of Fe-P, Ca-P and O- P, Pseudomonas spp. in presence of all P forms, and Trichoderma spp. in presence of Al-P, Ca-P and O-P. Compared with control, in vitro root exudates of intercropped tomato activated all P forms examined in this study, and increased soil available P was positively correlated with abundances of Bacillus, Pseudomonas and Trichoderma spp., and relative abundances of Massilia and Planomicrobium. The bacterial community structure and diversity was altered in intercropped tomato root exudates treatment as compared to rest of the treatments. These findings suggest the key role of root exudates in increased bioavailability of soil P in the intercropping of tomato and potato onion.

Antifungal activity of marine-derived Paenibacillus sp. PNM200 against Fusarium oxysporum f. sp. lycopersici, the causal agent of tomato vascular wilt

Marine-derived bacteria are known for producing a wide range of specialized metabolites with antifungal activity against phytopathogens. Assessing the marine bacteria's ability to establish themselves in the rhizosphere and inhibit soil-borne phytopathogens under in vivo conditions could provide evidence that supports their use for biological control, widening biological control agent’s isolation sources. The current study evaluated the antifungal activity of a marine-derived bacterial collection against the tomato phytopathogen Fusarium oxysporum f. sp. lycopersici (FOL). Out of 152 isolates, 28 Firmicutes strains showed antifungal activity against ten isolates of F. oxysporum in in vitro testing. From them, Paenibacillus sp. PNM200 was identified as a potential PGPR and as a biocontrol agent against Fusarium vascular wilt. PNM200 colonized the tomato plant root system, increased plant growth, and reduced vascular wilt severity on greenhouse assays. Interestingly, plants treated with the spent supernatants of PNM200 cultures did not show a decrease in vascular wilt severity. This result indicates that the ability of PNM200 to act as a biocontrol agent against FOL does not depend on the diffusible antifungal metabolites produced during the fermentation process. Other Paenibacillus sp. PNM200 traits like chemotactic response towards tomato root exudates, biofilm, IAA, enzyme activity, and phosphate solubilization may contribute to PNM200 rhizospheric competence and biological control ability. Overall, the obtained results suggest that marine-derived bacteria could be a new source of biological control agents for integrated pest management of soil-borne diseases like Fusarium vascular wilt.

Chitosan Induces Plant Hormones and Defenses in Tomato Root Exudates.

In this work, we use electrophysiological and metabolomic tools to determine the role of chitosan as plant defense elicitor in soil for preventing or manage root pests and diseases sustainably. Root exudates include a wide variety of molecules that plants and root microbiota use to communicate in the rhizosphere. Tomato plants were treated with chitosan. Root exudates from tomato plants were analyzed at 3, 10, 20, and 30 days after planting (dap). We found, using high performance liquid chromatography (HPLC) and excitation emission matrix (EEM) fluorescence, that chitosan induces plant hormones, lipid signaling and defense compounds in tomato root exudates, including phenolics. High doses of chitosan induce membrane depolarization and affect membrane integrity. 1H-NMR showed the dynamic of exudation, detecting the largest number of signals in 20 dap root exudates. Root exudates from plants irrigated with chitosan inhibit ca. twofold growth kinetics of the tomato root parasitic fungus Fusarium oxysporum f. sp. radicis-lycopersici. and reduced ca. 1.5-fold egg hatching of the root-knot nematode Meloidogyne javanica.