Tolperisone Research Articles

Ефективність та безпека препарату Мідокалм у лікуванні постінсультної спастичності

Стаття присвячена проблемі спастичності у хворих після інсульту. Подані результати дослідження клінічної ефективності та безпеки лікування постінсультної спастичності та больового синдрому, пов’язаного з нею, препаратом Мідокалм. Отримані результати свідчать про те, що включення Мідокалму в комплексну терапію наслідків ішемічного інсульту призводить до збільшення м’язової сили та обсягу рухів у паретичних кінцівках, покращує адаптацію хворих із руховими порушеннями після перенесеного церебрального інсульту.

Применение толперизона в практике травматолога-ортопеда

Применение толперизона в практике травматолога-ортопеда

Multidimensional voltammetry: Four-way multivariate calibration with third-order differential pulse voltammetric data for multi-analyte quantification in the presence of uncalibrated interferences

A four-way multivariate calibration approach based on the combination of differential pulse voltammetric (DPV) data and four-way algorithms is described for the first time. To achieve this goal, the DPV response of each sample was recorded thirty-six times. Six current-potential matrices were recorded at six different pulse durations. Each matrix consists of six vectors which have been recorded at six different pulse heights. The three-way data array obtained for the calibration set and for each of the test samples were joined into a single four-way data array. The recorded four-way data array was nonlinear, thus, the non-linearities were tackled by potential shift correction using correlation-optimized warping (COW) algorithm and subsequently was analyzed with unfolded-partial least squares/residual trilinearization (U-PLS/RTL) and multi-way-PLS/RTL (N-PLS/RTL) as third-order multivariate calibration algorithms. A comprehensive and systematic strategy for comparing the performance of the two algorithms was presented in this work, in particular with a view of practical applications. This comparison was developed to identify which algorithm offers the best predictions for the simultaneous determination of levodopa (LD), carbidopa (CD), methyldopa (MD), acetaminophen (AC), tramadol (TRA), lidocaine (LC), tolperisone (TOP), ofloxacin (OF), levofloxacin (LOF), and norfloxacin (NOF) in the presence of benserazide (BA), dopamine (DP), and ciprofloxacin (COF) as uncalibrated interferences using a multi-walled carbon nanotubes modified glassy carbon electrode (MWCNTs/GCE). This study demonstrated the more superiority of U-PLS/RTL to resolve the complex systems. The results of applying U-PLS/RTL for the simultaneous determination of the studied analytes in human serum samples as experimental cases were also encouraging.

Enantiomeric separation of tolperisone and eperisone by reversed‐phase HPLC with cellulose tris(3‐chloro‐4‐methylphenylcarbamate)‐coated chiral column

Enantiomeric separations of centrally acting muscle relaxants, that is, tolperisone (TOL) and eperisone (EP), that are marketed as racemates were investigated by reversed-phase high-performance liquid chromatography (HPLC) on a polysaccharide-based chiral column. Both TOL and EP are basic drugs because they contain a tertiary amino group and have similar chemical structures with the exception of the p-methylphenyl and p-ethylphenyl groups in TOL and EP, respectively. A reversed-phase chiral column, that is, a Chiralcel OZ-RH column, which bears cellulose tris(3-chloro-4-methylphenylcarbamate) as the chiral moiety, was effective for the enantiomeric separation of TOL and EP enantiomers. The separation factor and resolution values obtained for TOL were 1.22 and 1.66, respectively, and those for EP were 1.21 and 2.24, respectively, using a 20 mm ammonium acetate in H2 O (pH 8.0 and 7.0, respectively)-CH3 CN (70:30) mobile phase. Using the proposed HPLC conditions, it was found that (R)-TOL eluted faster than (S)-TOL, as revealed by the optical rotation and circular dichroism spectroscopy. In contrast, EP was easily racemized under the experimental conditions, and hence, the elution order was not determined.

Simultaneous determination of tolperisone and lidocaine by high performance liquid chromatography.

A reversed phase high performance liquid chromatographic (RP-HPLC) method for the simultaneous determination of tolperisone (TP) and lidocaine (LD) has been developed. The drugs were separated on a column (4.60 x 250 mm(2)) Spherisorb ODS (5 microm) using 5.5% triethylamine in 70/30 v/v acetonitrile/water as mobile phase 0.7 ml min(-1)and UV detection at 254 nm. The detection limits for Tolperisone hydrochloride (TP-HCl) and lidocaine hydrochloride (LD-HCl) were 0.20 ng/20 microl and 100 ng/20 microl and the quantitation limits were 0.50 ng/20 microl and 250 ng/20 microl, respectively. Linear calibration curves over the ranges of 1-10, 10-100 and 150-500 microg ml(-1) for TP-HCl and 10-500 microg ml(-1) for LD-HCl were established. Different calibration slopes were found for TP probably owing to changes in refractive index due to increase in TP concentration. The average recoveries of the added TP in the samples (TP-HCl tablets and injection liquid). A solutions spiked with standard TP-HCl were 99.9 and 99.7% with the RSD (n=11) of 0.66 and 0.67%, respectively. The average recovery of the added LD in the sample (injection) spiked with standard LD-HCl was 98.9% with the RSD (n=11) of 0.59%. The proposed method has been applied to the determination of TP-HCl and LD-HCl in commercial products available in Thailand. Comparative determination of TP by UV spectrophotometry and LD by colorimetry were also carried out. The results obtained by both methods were in good agreement of those obtained by the proposed method verified by using t-test. The proposed RP-HPLC method is simple, accurate, reproducible and suitable for routine analysis.