Tobacco Products Research Articles

Identifying SSR/InDel loci related to tobacco bacterial wilt resistance using association mapping

Identifying molecular markers linked to tobacco bacterial wilt resistance is crucial for developing resistant tobacco varieties, thereby enhancing tobacco production and quality. In this two-year study, we evaluated the tobacco bacterial wilt disease index (TBWDI) in a mapping population of 78 tobacco accessions using SSR/InDel markers across 1377 marker loci. Two association models, GLM_Q and MLM_Q + Kinship, were used for association analysis. By considering multiple environments, selection thresholds, and phenotype values, we identified 19 reliable marker loci (P = 7.07-E05–4.98-E02) that explained 5.37–19.10 % of the phenotypic variation. Among these, we selected 11 accessions with high resistance to tobacco bacterial wilt, each containing at least one excellent locus. The models predicted five hybrid combinations whose offspring aggregated additional excellent loci. Additionally, one locus was identified during quantitative trait loci mapping, with accessions carrying this locus exhibiting significantly different TBWDI values from those without it, confirming its reliability and stability. A candidate gene (LOC107795335) was also identified downstream at 17.58 Kbp of the locus. Our study pinpointed reliable loci associated with bacterial wilt resistance, identified important materials for breeding resistant tobacco varieities, and predicted the best hybrid combinations for low disease indexes. The results of our study offer invaluable theoretical insights for breeding varieties with high and stable resistance.

Use of tobacco price-minimization strategies among public housing residents compared to U.S. low-income adults

BackgroundPrice minimization strategies (PMS) are ways for people to save money on tobacco purchases especially among those of lower socioeconomic status. This study assesses PMS and coupon receipt among public housing residents compared to U.S. low-income adults. MethodsData were from adults who currently use tobacco and live in District of Columbia Housing Authority (DCHA) public housing (n=270) and a US nationally representative sample of low-income adults who currently use tobacco (n=820). We examined the prevalence of PMS use across demographic characteristics and smoking behaviors, and qualitatively compared them across the two datasets. ResultsMost DCHA resident participants (84.2 %) and US low-income adults who currently use tobacco (91.5 %) recently used at least one PMS to save money on tobacco. The top-three most common strategies among DCHA residents were saving cigarettes to finish later (35.6 %), smoking fewer cigarettes (32.2 %), and finding cheaper places to buy cigarettes (30.4 %), while among U.S. low-income adults these strategies were using coupons or promotions (62.7 %), purchasing by bulk (55.5 %), and finding cheaper places to buy tobacco products (53.6 %). People who lightly smoke in DCHA were more likely than people who heavily smoke to use PMS in general (25.0 % vs. 13.7 %) and smoke fewer cigarettes to save money (35.8 % vs. 19.2 %). US low-income people who lightly smoke were more likely than people who heavily smoke to cut back on tobacco use (61.9 % vs. 39.8 %). ConclusionMost U.S. low-income individuals and DCHA residents who smoke engaged in PMS. Regulating these strategies while supporting tobacco use cessation may reduce the impact of tobacco in these populations.

Mapping of tobacco conversion characteristics in electrically heated systems: effect of air and temperatures on the onset of combustion and formation of volatile species

The understanding of tobacco pyrolysis and oxidation mechanisms is an important topic for tobacco science in order to reduce the emissions of toxic species and to better control the conversion of tobacco in electrically heated tobacco products (EHTPs) by avoiding combustion. In this work, we have instrumented experimental Tobacco Heating Devices (expTHD) with a micro-positioning system of a thin thermocouple. Each expTHD has a pre-programmed set temperature (between 250 and 550°C) to be able to investigate the influence of temperature on tobacco conversion characteristics and emissions. Puffing cycles were conducted under air or N2 in order to understand the effect of oxygen on tobacco conversion and the resulting emissions. Electrically Heated Tobacco Products (EHTPs) were heated by the expTHD to the different final set temperatures, and puffs were drawn according to the specified puffing cycles. The conversion of tobacco becomes clearly exothermic with air from 400°C after 8 puffs, indicating that combustion was triggered. Below a set temperature of 400°C, the tobacco conversion is always net endothermic, which includes the tobacco temperatures of commercially available Tobacco Heating Systems (THS) during operation (typically lower than 325°C). Furthermore, the volatiles emitted during the puffing cycles were sampled in cold impingers. PAHs (naphthalene, phenanthrene, pyrene, and benzo[a]pyrene) were quantified by HPLC-UV Fluorescence. Benzene and toluene were quantified by GC/MS. Gases (CO, CO2, CH4, H2) were quantified by FTIR and µGC. The mass yields of all these species are presented as a function of the final set temperature of the expTHD and of the carrier gas used during the puffs (air or N2). CO2 yields are higher for air than for N2 even at 250°C, highlighting some low temperature oxidation reactions, but they did not lead to a detectable exothermic regime. A jump in CO formation is observed from 400°C under air, indicating combustion of the tobacco. Benzene and PAHs are promoted by air (compared to N2) from 400°C (heater set temperature). Therefore, air does not promote the formation of these species during the operation of the commercially available THS.

The impact of the introduction of tobacco product plain packaging on consumer responses in Ireland: a real-world policy evaluation stratified by socioeconomic groups.

Smoking prevalence remains high in Europe and widening socioeconomic group differences are driving health inequalities. While plain packaging policies disrupt tobacco industry tactics that sustain smoking, evidence of their equity impact is sparse. This study evaluated the implementation of plain packaging in Ireland in 2018 on consumer responses, overall and by the socioeconomic group. Consecutive nationally representative cross-sectional surveys (2018, n = 7701 and 2019, n = 7382) measured changes in 13 consumer responses among respondents who smoked across three domains: product appeal, health warnings effectiveness, and perceived harmfulness of smoking. Multiple logistic regression-derived adjusted odds ratios with 95% confidence intervals to compare responses post- versus pre-implementation adjusting for age, gender, educational level, and heaviness of smoking. A stratified analysis examined changes by socioeconomic group indexed using educational level. There were statistically significant changes in consumer responses to plain packaging policy implementation across 7/13 outcomes studied. Five changes were aligned with expected policy impacts (2/6 product appeal outcomes and 3/4 health warning effectiveness outcomes). Two responses were also observed which were not expected policy impacts (1 appeal-related and 1 perceived harm-related outcome). There was no change in five outcomes. Differences in consumer responses between educational groups were generally small, mixed in nature, and indistinguishable when interval estimates of effect were compared. Implementation of plain packaging in Ireland had intended impacts on consumer responses. Including plain packaging requirements in revising the European Union's legislative frameworks for tobacco control will help build progress towards a Tobacco-Free Europe without exacerbating smoking inequalities.

Gaps in Awareness of the United States Minimum Legal Age of E-Cigarette and Cigarette Sales: Implications for Public Health Messaging

PurposeIn 2019, the United States raised the minimum legal age (MLA) of sales for all tobacco products from 18 to 21. Public awareness of the federal MLA of tobacco sales overall and by demographics is understudied. MethodsA national, cross-sectional 2023 survey in which 6,098 participants aged 13–40 years responded to the question, “at which age is it legal to purchase [e-cigarettes; cigarettes; alcohol] in the United States?” Participants also reported age, sex, race/ethnicity, and prior use of each substance. ResultsSixty-one percent, 64.6%, and 88.7% correctly identified the MLA of sales for e-cigarettes, cigarettes, and alcohol, respectively. Differences in awareness of the MLA were found by age, sex, race/ethnicity, and prior use status. DiscussionGaps in awareness of the MLA of tobacco sales, particularly among adolescents and adults, suggest messaging regarding the federal Tobacco 21 law is needed.

Heterogeneous Association Between E-Cigarette Use and Diabetes Prevalence Among U.S. Adults

IntroductionE-cigarette use has increased among US adults. While the association between traditional cigarette smoking and the diabetes/prediabetes prevalence is well established, the relationship between e-cigarette use and these conditions remains unclear. This study examines the association between different types of cigarette use (including e-cigarettes alone, combustible cigarettes alone, and dual use) and the prevalence of prediabetes and diabetes among US adults. MethodsWe analyzed 1,285,783 observations from the 2020-2022 Behavioral Risk Factor Surveillance System surveys. Self-reported prediabetes and diabetes diagnoses were outcomes of interest. Propensity score matching with one-to-one nearest-neighbor matching was used to create balanced groups of smokers and non-smokers. Multivariable logistic regressions examined associations between self-reported smoking status and outcomes. We also conducted subgroup analyses to assess potential variations in these associations. Analyses were performed in September-December 2023. ResultsAfter propensity score matching and adjusting for covariates, sole e-cigarette users (adjusted odds ratio [AOR], 1.07; 95% CI, 1.03-1.11) and dual users (AOR, 1.28; 95% CI, 1.23-1.33) had higher odds of prediabetes diagnosis compared with never users. Sole e-cigarette use was not associated with diabetes risk, but dual users showed higher odds for diabetes diagnosis (AOR, 1.09; 95% CI, 1.06-1.11) versus never users. E-cigarette use was associated with varying odds of prediabetes and diabetes across different demographic groups. ConclusionsThis cross-sectional analysis found that e-cigarette use is associated with higher odds of prediabetes, with dual use potentially linked to increased odds of both prediabetes and diabetes. However, due to study limitations, including its cross-sectional design, self-reported data, and unmeasured confounders, these findings should be interpreted cautiously. As causal relationships and temporal sequences cannot be established, large prospective studies are needed to explore the metabolic health implications of emerging tobacco product use.

Из истории развития табаководства как важнейшей отрасли торгового земледелия в губер-ниях Южного Кавказа во второй половине XIX – начале XX века

The article focuses on the evolution of tobacco cultivation in the South Caucasus over the research period. The author investigates the spread of this technological culture throughout all provinces in the specified region. Information is provided on the quantity of land occupied for tobacco cultiva-tion, tobacco types, industrial firms and their owners, tobacco products produced, and tobacco sales locations. The author examines the many causes of the shift in demand for tobacco products over this historical time, as well as the unequal level of tobacco production. The essay discusses the excellent conditions for the growth of tobacco production in the South Caucasus, which eventually became commodities. The report also underlined the Russian government’s interest in spreading this crop, which was the primary raw material for the tobacco sector. The government, which contributed to the region’s colonial exploitation by the Russian and foreign bourgeoisie, benefited financially from the expansion of the cultivation of technologi-cal and precious crops. The author finds that the tobacco business in the South Caucasus evolved, improved, and gradually adopted the characteristics of commercial agriculture, which included commodity rela-tions. It is emphasized that the quick expansion of commercial agriculture is closely tied to the emergence of capitalist relationships in agriculture.

Salivary cysteine levels as a potential biochemical indicator of oral cancer risk in tobacco consumers.

Aim: Oral cancer is the leading cause of mortality, with a survival rate of less than 5 years, and is predominantly influenced by tobacco mutagens. Invasive diagnostic methods hinder early detection of oral cancer biomarkers. The present study performed salivary biochemical analysis for early oral cancer screening in tobacco consumers.Materials & methods: Three study groups included healthy controls (n=25), tobacco users (n=25) and oral cancer patients (n=25). Salivary total protein, amylase, TNF-α and amino acid levels were evaluated using enzymatic tests, Enzyme linked Immunosorbent Assay (ELISA) and High-Performance Liquid Chromatography (HPLC).Results: Compared with healthy controls, salivary total protein and TNF-αlevels were significantly (p=0.04) higher in oral cancer patients. Salivary amylase levels were significantly lower in tobacco smokers (p=0.02) and higher in oral cancer patients (p=0.01). Interestingly, the amino acid cysteine concentration was significantly higher (p=0.02) in tobacco consumers (62.5±10) than in healthy controls (116.1±28).Conclusion: In high-risk populations, such as tobacco users, salivary biochemical analysis can serve as a promising noninvasive diagnostic method for early oral cancer screening. As a salivary biomarker, the amino acid cysteine exhibits potential as a means of detecting the progression of oral cancer in individuals who consume tobacco.

Tobacco Products Use and Policy for Tobacco Cessation

Tobacco Products Use and Policy for Tobacco Cessation

Bio-insecticidal Efficacy of Beauveria bassiana Vuill. Strains against Tettigonia viridissima L. in Tobacco

The green grasshopper (Tettigonia viridissima L.) is a species which attacks tobacco plants. The purpose of the present study was to establish the bio-insecticidal efficacy of entomopathogenic fungi Beauveria bassiana Vuill. strains 214, 644, and 733 on the growth and development of T. viridissima in tobacco. The research was conducted under laboratory conditions. The study found insecticidal effects of the B. bassiana strains 214, 644, and 733 against T. viridissima. Surviving of larvae was monitored at 5-day intervals until the 15th day after treatment with a spore suspension of the tested Beauveria strains. The highest virulence of the tested fungi was shown by strain 644, followed by strains 214 and 733. On the 5th day after treatment, no efficacy against T. viridissima larvae was detected. On the 10th day post-treatment strain 644 showed 66.7% efficacy, while efficacy against larvae of grasshoppers for the other two strains 214 and 733 was significantly lower: 33.3%. Efficacy gradually increased and on the 15th day post-treatment 100% mortality of T. viridissima was observed for all isolates. The results obtained from the virulence test of B. bassiana 214, 644, and 733 ascertain their application as biocontrol agents in tobacco production under field conditions.

Just How Profitable Is Illicit Tobacco in Australia?

In the past 12 months, the southern Australian state of Victoria has been in the midst of what may be a ‘turf war’ between rival organised crime groups for the control of the sale of illicit tobacco products. While organised crime has been more traditionally associated with the distribution and sale of narcotic drugs, illegal gaming venues, prostitution services or human trafficking, recent events might suggest that distribution of untaxed tobacco is now another source of income for criminal gangs. This paper does not look to connect organised crime with illicit tobacco but rather looks at the extent of potential profits to be made from sourcing and distributing tobacco products on which the appropriate federal duties and taxes have not been paid, and whether this could be a possible ‘driver’ in attracting organised crime to the product. Using publicly available data, this paper will propose a methodology and use that methodology to gauge the potential profitability in relation to a container of imported illicit cigarettes.

Gender-Based Socioeconomic Inequality of Electronic Cigarettes and Heated Tobacco Products in Korea

Gender-Based Socioeconomic Inequality of Electronic Cigarettes and Heated Tobacco Products in Korea

Exposure to smoking tobacco, roll your own tobacco, heated tobacco products, and e-cigarette advertising in 28 European countries.

Exposure to smoking tobacco, roll your own tobacco, heated tobacco products, and e-cigarette advertising in 28 European countries.

Comparative evaluation of cigarette smoke and a heated tobacco product on microglial toxicity, oxidative stress and inflammatory response

BackgroundTobacco smoking is the leading cause of preventable death and disease worldwide, with over 8 million annual deaths attributed to cigarette smoking. This study investigates the impact of cigarette smoke and heated tobacco products (HTPs) on microglial function, focusing on toxicological profiles, inflammatory responses, and oxidative stress using ISO standard and clinically relevant conditions of exposure.MethodsWe assessed cell viability, reactive oxygen species (ROS) production, lipid peroxidation, mitochondrial function, unfolded protein response, and inflammation in human microglial cells (HMC3) exposed to cigarette smoke, HTP aerosol or nicotine.ResultsOur findings show that cigarette smoke significantly reduces microglial viability, increases ROS formation, induces lipid peroxidation, and reduces intracellular glutathione levels. Cigarette smoke also alters the expression of genes involved in mitochondrial dynamics and biogenesis, leading to mitochondrial dysfunction. Additionally, cigarette smoke impairs the unfolded protein response, activates the NF-κB pathway, and induces a pro-inflammatory state characterized by increased TNF and IL-18 expression. Furthermore, cigarette smoke causes DNA damage and decreases the expression of the aging marker Klotho β. In contrast, HTP, exhibited a lesser degree of microglial toxicity, with reduced ROS production, lipid peroxidation, and mitochondrial dysfunction compared to conventional cigarettes.ConclusionThese results highlight the differential toxicological profile of cigarette smoke and HTP on microglial cells, suggesting a potential harm reduction strategy for neurodegenerative disease for smokers unwilling or unable to quit.

Study of Knowledge, Attitudes and Practices Related to Smoking Among Health Professionals in Public Hospitals in the Department of Dakar in 2021

Introduction: In Senegal, previous studies showed a prevalence of smoking among health professionals of between 11.6 and 12.8%. The general objective was to study smoking among health professionals in public hospitals in the department of Dakar. This was a cross-sectional study with a descriptive and analytical aim, the data collection phase of which took place from October 11 to November 23, 2021. The study population consisted of medical and paramedical staff. We collected 262 cases, i.e. a non-participation rate of 12.67%. Results: The average age of the health workers surveyed was 28.19 %. There were 148 men and women with a sex ratio of 1.30. Thus, 68.3% were single. During our study, the health workers at Abass NDAO Hospital were 110 (42%), 80 (30.5%) at the Principal Hospital in Dakar and 72 (27.5%) at HOGGY. The majority of the workers surveyed (69 cases, 26.3%) were DES or 7th year medical students. The least represented professional category was that of nursing assistants. Thus, 3.4% of the health workers surveyed were current tobacco users compared to 10.2% (26 health workers) who were former users. The average age at which these workers first used tobacco was 16.5 ± 2.5 years. Within the study population, 86% (225 workers) had never used tobacco. Smoked tobacco was the only form used by the subjects surveyed. The average consumption of smoked tobacco was 3.5 ± 0.51 times per day. However, the last attempt to quit was more than a year ago for the majority (5/7) of consumer health workers. The main reason for this attempt (failed) was awareness of the dangers of tobacco consumption (5/7). The main reason (05/07) that justified their tobacco consumption was stress management. All the workers surveyed were unanimous on the dangers of tobacco consumption on health. The consequences of tobacco most cited by the health workers surveyed were lung cancer (224 responses or 85.5% of respondents), COPD (205 or 78.24%) and strokes (165 or 62.98%). Male health workers were more likely to have good knowledge about smoking than female health workers. The agents thus surveyed had for 73.7% (i.e. 193 people) of them an insufficient or weak attitude towards smoking. Conclusion: At the end of our study and in light of the conclusions reached, we make the following suggestions to the health authorities: To organize communication-education sessions during World Tobacco Day.

Development of an LC-HRMS non-targeted method for comprehensive profiling of the exposome of nicotine and tobacco product users – A showcase for cigarette smokers

The global prevalence of electronic cigarettes, heated tobacco products, and other smokeless alternatives has grown significantly in the last ten years. These products have been suggested as combustion-free alternatives for conventional tobacco products like cigarettes, aiming to reduce the negative health impacts associated with smoking. However, the impact of those products on the health and safety of the general population are still unclear, as the absolute exposure from those products has not been thoroughly studied, yet. In this project, a non-targeted LC-HRMS method was developed comprising four different analytical modes for the investigation of the exposure profile in urine of the product users. The method is characterized by its high sensitivity and reproducibility, as shown during method validation. As a proof of concept, we first applied this method to detect significant differences in biomarkers of exposure (BoEs) between smokers and non-smokers. We observed a total of 171 BoEs significantly elevated in smokers, including several well-known biomarkers of smoke exposure like nicotine and its metabolites, mercapturic acid derivatives, and phenolic compounds. Some of the detected biomarkers are present at low ng/mL concentrations in urine, proving the high sensitivity needed for a holistic exploration of the exposome. Moreover, we were able to identify BoEs that have not been reported previously for smoking, such as 2,6-dimethoxyphenol and 7-methyl-1-naphthol glucuronide.

The effect of rising e-cigarette usage among young people on public health and physical activity

Introduction: Electronic cigarettes (e-cigarettes/ENDS) are regarded by many as a safe alternative to traditional cigarettes. This article will present a review of the current knowledge regarding the effects and impact of electronic cigarettes on health and the risk of addiction in adolescents. Methods: We selected articles with an unlimited search period in several databases, including PubMed, Google Scholar, Web of Science and the websites of the Polish government. Only articles with free full text were included in the review. There were no restrictions based on article type. Conclusions: The increasing consumption of e-cigarettes (ENDS) among young people in Poland is emerging as a significant public health concern. The high addictive potential of nicotine, coupled with the toxicity of aerosols and the multitude of ways in which manufacturers make their products more appealing, gives rise to the risk of an increase in future patients with impaired performance in sports, and consequently with cardiovascular and respiratory diseases, as well as the necessity to combat addiction. Reducing and preventing the use of tobacco products by children and adolescents represents one of the most significant challenges currently facing the public health sector in Poland.

Understanding Potential Mechanisms of Vaping Prevention Messages: A Mediation Analysis of the Real Cost Campaign Advertisements.

The U.S. Food and Drug Administration (FDA) developed a public education campaign, The Real Cost, that reduced youth susceptibility to tobacco product use. We sought to identify the mechanisms that may underlie the impact of The Real Cost ads on susceptibility to vaping to inform youth tobacco prevention campaigns. Our online randomized controlled trial (clinicaltrials.gov, identifier NCT04836455) examined a large sample of U.S. adolescents (n = 1,348) who had multiple exposures to Real Cost ads or control videos over a 3-week period in 2021. To examine potential mediating pathways between The Real Cost ads and susceptibility to vaping, we examined theory-based psychosocial and message-related variables. The largest impact of The Real Cost ads on susceptibility was via more negative attitudes toward vaping (βa*βb = -0.16; 95% confidence interval [CI] = [-0.25, -0.06]). Other mediation paths were via improved health harm risk beliefs (βa*βb = -0.08; 95% CI = [-0.13, -0.04]), addiction risk beliefs (βa*βb = -0.04; 95% CI = [-0.06, -0.01]), injunctive norms against vaping (βa*βb = -0.05; 95% CI = [-0.09, -0.02]), negative affect (βa*βb = -0.05; 95% CI = [-0.08, -0.02]), and cognitive elaboration (βa*βb = -0.03; 95% CI = [-0.05, -0.003]). Our findings suggest that ads that target negative attitudes may decrease susceptibility to vaping among youth. Our findings also introduce normative pressure as a novel factor that may be important for vaping prevention messages.

The Effects of Smoking on Dental Implant Failure: A Current Literature Update.

Background: This systematic review assesses the current literature (2020-2024) evaluating the impact of smoking on dental implant failure rates. Methods: A non-funded Pubmed database review was conducted according to PRISMA guidelines, and the results were tabulated to extract the study design, patient characteristics, follow-up time, comparison, outcome, and strengths and weaknesses, including risk of bias. This review included 33 studies with 29,519 implants placed in over 18,301 patients. We included prospective and retrospective clinical studies, randomized and non-randomized controlled trials, cohort studies, and observational studies that examined smoking's effects on implant failure rates. Studies had to classify individuals into two groups, smokers and non-smokers, with at least ten implants. Exclusions included reviews, case reports, experimental studies, guidelines, non-English publications, studies lacking comparative data on failure rates, those excluding smokers, and studies focusing on head and neck cancer patients or specialized implants. Results: Our findings indicate a significant correlation in 25 out of 33 studies between smoking and increased implant failure rates, affecting both early and late stages of implant integration and survival as well as revealing a dose-response relationship, with higher daily cigarette consumption significantly increasing the risk of implant failure. Conclusions: This review highlights the importance of smoking cessation efforts, patient education, and tailored patient care in dental implantology. Future research should explore the effects of smoking frequency and alternative tobacco products, such as e-cigarettes, aiming to improve success rates among smokers.

Influence of body fat on heart rate variability in healthy females.

To evaluate the effect of central obesity indices in healthy females on cardiac autonomic activity, using heart rate variability as an index reflecting the cardiac autonomic balance. The cross-sectional study was conducted at the Department of Physiology, College of Medicine, Mustansiriyah University, Baghdad, Iraq, from September 2020 to April 2021, and comprised adult healthy women having a regular menstrual cycle who were not consumers of tobacco, alcohol or any medication. Waist circumference, waist-hip ratio, waist-height ratio and lipid accumulation product measurements were noted for each subject. Repeated blood pressure and heart rate measurements were taken on the brachial artery of the right arm until stable values were achieved. Heart rate variability was based on electrocardiogram data related to R-R interval. Data was analysed using GraphPad InStat version 3.06. There were 53 women with mean age 37.4±10.5 years. Low-frequency power normalised units at the highest two quintiles of waist circumference was significantly higher than the lowest two quintiles (p<0.03), and significantly and positively correlated with waist-hip ratio (p<0.02) and waist-height ratio (p<0.05). At the highest two quintiles of lipid accumulation product, low-frequency power normalised units value was significantly higher than at the lowest two quintiles (p<0.02). Increased waist circumference, waist-height ratio, waist-hip ratio and lipid accumulation product values were significantly associated with high sympathetic tone.