Tissues Of Broiler Chickens Research Articles

Potential and Metabolic Impacts of Double Enrichments of Docosahexaenoic Acid and 25-Hydroxy Vitamin D3 in Tissues of Broiler Chickens

BackgroundChicken may be enriched with 25-hydroxy D3 [25(OH)D3] and docosahexaenoic acid (DHA) to enhance the dietary intake of the public. ObjectivesTwo experiments (Expt.) were conducted to determine the potential and metabolic impacts of enriching both DHA and 25(OH)D3 in the tissues of broiler chickens. MethodsIn Expt. 1, 144 chicks (6 cages/treatment and 6 birds/cage) were fed a corn–soybean meal basal diet (BD), BD + 10,000 IU 25(OH)D3/kg [BD + 25(OH)D3], BD + 1% DHA-rich Aurantiochytrium (1.2 g DHA/kg; BD + DHA), or BD + 25(OH)D3+DHA for 6 wk. In Expt. 2, 180 chicks were fed the BD, BD + DHA-rich microalgal oil (1.5–3.0 g DHA/kg, BD + DHA), BD + DHA + eicosapentaenoic acid (EPA)-rich microalgae (0.3–0.6 g EPA/kg, BD + DHA + EPA), BD + DHA + 25(OH)D3 [6000 to 12,000 IU/kg diet; BD + DHA + 25(OH)D3], and BD + DHA + EPA + 25(OH)D3 for 6 wk. ResultsSupranutrition of these 2 nutrients resulted in 57–62 mg DHA and 1.9–3.3 μg of 25(OH)D3/100 g of breast or thigh muscles. The DHA enrichment was independent of dietary EPA or 25(OH)D3, but that of 25(OH)D3 in the liver was decreased (68%, P < 0.05) by dietary DHA in Expt. 1. Compared with BD, BD + 25(OH)D3 enhanced (P < 0.05) gene expression related to D3 absorption (scavenger receptor class B type 1 and Niemann-pick c1 like 1) in the liver and D3 degradation (cytochrome P450 24A1) in the breast, and decreased mRNA or protein concentrations of vitamin D binding protein in the adipose tissue or thigh muscle. Supranutrition of DHA decreased mRNA concentrations of lipid metabolism-related genes (fatty acid desaturase 1,2, ELOVL fatty acid elongase 5, fatty acid desaturase 2, fatty acid synthase, and sterol regulatory element-binding protein 1). ConclusionsBoth DHA and 25(OH)D3 were enriched in the muscles up to meeting 50%–100% of the suggested intakes of these nutrients by consuming 2 servings of 100 g of fortified chicken. The enrichments altered gene expression related to lipid biosynthesis and vitamin D transport or storage.

Establishment of withdrawal time and analysis of tiamulin residue in edible tissues of broiler chickens administered in drinking water

Establishment of withdrawal time and analysis of tiamulin residue in edible tissues of broiler chickens administered in drinking water

Comprehensive Screening of Salinomycin in Feed and Its Residues in Poultry Tissues Using Microbial Inhibition Tests Coupled to Enzyme-Linked Immunosorbent Assay (ELISA).

Salinomycin is a coccidiostat approved as a feed additive for the prevention of coccidiosis in poultry. Official control of its residues is set by the Commission Delegated Regulation (EU) 2022/1644. The aim of our study was to assess the suitability of three microbial inhibition tests (MITs), Premi®Test, Explorer 2.0, and the Screening Test for Antibiotic Residues (STAR) linked to the enzyme-linked immunosorbent assay (ELISA), for the screening of salinomycin residues in the tissues of broiler chickens (breast and thigh muscle, heart, liver, gizzard, kidneys, lungs, spleen, skin, and fat) fed commercially produced feed containing 70 mg.kg-1 of salinomycin in the complete feed. The first residue screening (Sampling A) was performed on the last day of administration of the salinomycin-medicated feed (day 30), and the second screening (Sampling B) was performed on the day of slaughter (day 37) after the expiry of the withdrawal period with the feeding of non-medicated feed. Based on the quantitative confirmation of salinomycin residues in the examined chicken tissues by the ELISA method (Sampling A from 0.025 to 0.241 mg.kg-1; Sampling B from 0.003 to 0.076 mg.kg-1), all the MITs with the preference of the bacterial strain Bacillus stearothermophilus var. calidolactis ATCC 10149 demonstrated the ability to detect the residues of salinomycin in the examined tissues of broiler chickens at the level of the maximum residue limits set from 0.015 to 0.150 mg.kg-1 by Commission Implementing Regulation (EU) 2017/1914 and confirmed the relevance of their sensitivity to the coccidiostat salinomycin.

Vitamin E Can Down-regulate Some of Apoptotic Genes Involved in Pulmonary Hypertension Syndrome in Broiler Chicken

Background: Ascites or pulmonary hypertension syndrome (PHS) is one of the significant problems in the poultry industry. Therefore, various studies have been conducted on its contributing factors. Objectives: This study aimed to investigate the role of vitamin E in reducing the mRNA levels of caspase-1 (CASP1), caspase-2 (CASP2), and caspase-3 (CASP3) genes involved in the apoptosis pathway. Methods: Ninety fast-growing 1-day-old chickens (Ross 308) were randomly assigned to three equal groups, including sham (basal diet), control (basal diet+1.5 mg/kg of triiodothyronine [T3]), and treatment group (basal diet+400 mg/kg of vitamin E+1.5 mg/kg of T3). To induce ascites, 1.5 mg/kg of T3 was added to basal diet from the seventh day to the end of the experiment. On the 21st and 49th days after rearing, 15 chicks from each group were randomly selected. The right ventricle/total ventricle weight ratio (RV/TV) and the expression levels of CASP1, CASP2, and CASP3 genes in the lung and right ventricle of all three groups of broiler chickens were measured and compared. Results: Although there was no significant difference between the three groups in terms of the RV/TV ratio on day 21 post-reared (P≥0.05), a significant decrease was detected in the vitamin E-receiving group compared to the control group with respect to the RV/TV ratio on day 49 post-reared (P&lt;0.05). Also, vitamin E reduced the relative expression of CASP1, CASP2, and CASP3 at 49 days of age in the lung and heart tissues of broiler chickens with ascites (P&lt;0.05). Conclusion: Based on the results of this study, it seems that vitamin E can reduce some apoptosis genes (CASP1, CASP2, and CASP3) associated with pulmonary hypertension in broilers.

Establishment of withdrawal time and analysis of didecyldimethylammonium chloride (DDAC) residue in edible tissues of broiler chickens by spraying DDAC disinfectant

Establishment of withdrawal time and analysis of didecyldimethylammonium chloride (DDAC) residue in edible tissues of broiler chickens by spraying DDAC disinfectant

The Effect of the Supplementation of Humic Substances and Fermented Products in the Feed on the Content of Salinomycin Residues in Poultry Tissues.

The presence of antimicrobial residues in products of animal origin is a constant problem for consumer health. The aim of this study was to observe the effect of the addition of humic substances (H), fermented products (F) and a mixture of both (FH) to feed supplemented with the coccidiostat salinomycin, compared with a control group (C), on the content of salinomycin residues in the edible tissues of broiler chickens using two microbial inhibition screening methods, Explorer 2.0 test and the Screening Test for Antibiotic Residues (STAR), and a confirmatory competitive enzyme immunoassay analysis (Salinomycin ELISA Kit). The results of the microbial inhibition tests showed a gradual decline in the positive results in the tissue samples from the last day of salinomycin administration (30th day) tothe last day of fattening (37th day, day of slaughter) in group C and no positive results in the tissue samples from experimental groups H, F and FH slaughtered on the last day of fattening. Using the Salinomycin ELISA Kit, salinomycin was detected in the chicken muscle tissues of all the control and experimental groups. However, no sample from any group contained salinomycin at a concentration exceeding the maximum residue limits set by European law. The high level of significance (p < 0.001) confirmed the positive influence of the administration of humic substances and fermented products on the content of salinomycin residues in chicken tissues.

Assessment of Enrofloxacin Usage and Residue Levels of Enrofloxacin-Ciprofloxacin in Breast and Liver Tissues of Commercial Broilers Sold in Kampala-Uganda.

Human exposure to veterinary drugs like fluoroquinolones occurs due to the presence of their residues in foods from animal sources in varying concentrations. The existence of antibiotic residues in foodstuffs can pose great public health problems to consumers. This study aimed to assess enrofloxacin use patterns and assess residue levels of enrofloxacin/ciprofloxacin in breast muscle and liver tissues of broiler chickens sold for consumption in Kampala capital city. This was a cross-sectional study that involved both field survey and laboratory-based methods. The field study involved the use of qualitative and semi-quantitative data collection tools to interview 34 broiler farmers and 10 veterinary drugs vendors. For the determination of enrofloxacin/ciprofloxacin levels, 68 chicken breast and liver tissue samples were collected from main markets in Kampala over one month and analyzed using HPLC-UV. Enrofloxacin was the most used antibiotic (100%) for the management of poultry diseases, majorly respiratory diseases (100%), salmonella infections (40%), and disease prevention (60%). Over 76% of the farmers knew the meat withdrawal time (WDT) for enrofloxacin, but none of them adhered to this. Over 70% of the farmers reported that the veterinary drugs vendors were not providing meat WDT information. Enrofloxacin/ciprofloxacin residues were identified in 35.3% (12/34) of the muscle and 38.2% (13/34) of the liver tissues analyzed. Of those muscle and liver tissue samples that tested positive, 25% (3/12) and 38.5% (5/13) respectively had drug concentrations higher than the recommended Maximum Residue Limits. The overall mean enrofloxacin/ciprofloxacin concentration in the chicken muscle and liver tissues was 83.6 (±34.5) µg/kg and 171.5 (±75.9) µg/kg. This observed presence of enrofloxacin/ciprofloxacin levels above safety requirements is attributable to inadequate medicines use information provided by veterinary drugs vendors to farmers and also to the non-compliance of some farmers to meat WDT due to the economic implications.

Effect of In Ovo Administration of a Multi-Strain Probiotic and Zinc Glycine Chelate on Antioxidant Capacity and Selected Immune Parameters in Newly Hatched Chicks.

The aim of this study was to determine the effect of in ovo co-supplementation of chicken embryos with a multi-strain probiotic containing effective microorganisms and zinc glycine chelate on total antioxidant capacity; concentrations of sulfhydryl groups, bityrosine bridges, formylkynurenines, hydroperoxides, proteins, corticosterone, pro- and anti-inflammatory cytokines and heat shock proteins; and the activity of catalase and superoxide dismutase in the serum, yolk sac and tissues of broiler chickens at 12 h and at 7 days after hatching. The results indicate high SOD activity in the small and large intestines of chicks at 12 h post-hatch in the groups receiving the multi-strain probiotic and in the small intestine and yolk sac of birds receiving the multi-strain probiotic and Zn-Gly chelate. High concentrations of TNF-α and IFN-γ in the yolk sac and serum after in ovo administration of Zn-Gly chelate were observed 12 h after hatching. The use of a probiotic and a probiotic with Zn-Gly chelate increased the total antioxidant capacity in the tissues of chickens. It can be concluded that in ovo administration of a multi-strain probiotic and Zn-Gly chelate can maintain the oxidant/antioxidant balance in chickens and increase the defense capacity against oxidative stress.

Establishment of withdrawal time and analysis of ethopabate residue in edible tissues of broiler chickens administered in drinking water

Establishment of withdrawal time and analysis of ethopabate residue in edible tissues of broiler chickens administered in drinking water

Establishment of withdrawal time and analysis of amprolium residue in edible tissues of broiler chickens administered in drinking water

Establishment of withdrawal time and analysis of amprolium residue in edible tissues of broiler chickens administered in drinking water

Cellular Immune Responses in Lymphoid Tissues of Broiler Chickens Experimentally Infected with Necrotic Enteritis-Producing Clostridium perfringens Strains.

Host cellular responses against Clostridium perfringens (CP), the causative agent of necrotic enteritis (NE) in chickens, are poorly understood. In the present study, we first tested the NE-producing ability of seven netB+ CP strains (CP5, CP18, CP26, CP64, CP67, CP68, and NCNE-1), using an experimental infection model of broiler chickens. Evaluation of intestinal gross lesions showed that all the strains, except CP5, were able to produce NE, while CP26 and CP64 strains produced relatively more severe lesions when compared with other groups. Next, cellular responses in the cecal tonsil (CT), bursa of Fabricius, and spleen were evaluated in chickens infected with strains representing variation in the level of virulence, namely, avirulent CP5, virulent CP18, and a relatively more virulent CP26 strain. Immunophenotyping analysis showed that CT or splenic macrophage frequencies were significantly higher in CP18- and CP26-infected chickens compared with uninfected controls, while the frequencies of γδ T-cells and B-cells in the CT of CP26-infected chickens were significantly higher than those in the uninfected, CP5- or CP18-infected groups. The T-cell analysis showed that chickens infected with CP18 and CP26 had a significantly higher number of splenic CD4+ and CD8+ T-cells expressing CD44 and CD28 activation molecules, while CP26-infected chickens also had significantly increased CT frequency of these activated CD4+ and CD8+ T-cells when compared with uninfected or CP5-infected groups. Collectively, our findings suggested that cellular responses, including activation of T-cells, are selectively induced against virulent CP strains and that the NE-producing characteristics of this pathogen may influence the outcome of immunity to NE.

Transcriptome analysis of the uterovaginal junction containing sperm storage tubules in heat-stressed breeder hens

Sperm storage tubules (SSTs) in the uterovaginal junction (UVJ) of the oviduct are major sites of sperm storage after artificial insemination or mating. Female birds may regulate sperm motility in the UVJ. Heat stress can decrease the reproductive ability of broiler breeder hens. However, its effects on UVJ remain unclear. Changes in gene expression aid in understanding heat stress-affected molecular mechanisms. Herein, we wanted to conduct a comparative transcriptomic analysis to identify the differentially expressed genes (DEGs) in the UVJ of breeder hens under thermoneutral (23°C) and heat stress (36°C for 6 h) conditions. The results indicated that cloacal temperatures and respiratory rates were significantly increased in heat-stressed breeder hens (P < 0.05). Total RNA was extracted from the hen UVJ tissues containing SSTs after heat exposure. Transcriptome analysis identified 561 DEGs, including 181 upregulated DEGs containing heat shock protein (HSP) transcripts and 380 downregulated DEGs containing immune-related genes, such as interleukin 4-induced 1, radical S-adenosyl methionine domain containing 2, and 2'-5'-oligoadenylate synthetase like, in heat-stressed hens. Gene Ontology analysis revealed the significantly enriched terms involving HSPs. Kyoto Encyclopedia of Genes and Genomes analysis identified 9 significant pathways, including the protein processing in endoplasmic reticulum (11 genes including HSPs), neuroactive ligand-receptor interaction (13 genes including luteinizing hormone/choriogonadotropin receptor), biosynthesis of amino acids (4 genes including tyrosine aminotransferase), ferroptosis (3 genes including heme oxygenase 1), and nitrogen metabolism (carbonic anhydrase [CA]-12 and CA6) pathways. Protein-protein interaction network analysis of DEGs revealed 2 large networks, one containing upregulated HSPs and the other containing downregulated interferon-stimulating genes. Overall, heat stress inhibits innate immunity in the UVJ tissues of broiler chickens, and heat-stressed chickens protect their cells by increasing the expression levels of HSPs. The identified genes are potential candidates for further exploration of the UVJ in heat-stressed hens. The identified molecular pathways and networks increase our understanding of the sperm storage reservoirs (UVJ containing SSTs) within the reproductive tract and may be used to prevent heat stress-induced fertility loss in breeder hens.

Feasibility of including a phytobiotic containing cinnamon oil in the diet to reduce the occurrence of neurodegenerative changes in broiler chicken tissues

1. Adarsh A., Kanthesh B.M., Raghu N., Bharath C., 2020. Phytochemical screening and antimicrobial activity of "Cinnamon zeylanicum". Int. J. Pharm. Res. Innov. 13, 22–33, http://doi.org/10.13140/RG.2.2... CrossRef Google Scholar

Mucosal and systemic lymphoid immune responses against Clostridium perfringens strains with variable virulence in the production of necrotic enteritis in broiler chickens

ABSTRACT Necrotic enteritis (NE), caused by Clostridium perfringens, is an economically important disease of chickens. Although NE pathogenesis is moderately well studied, the host immune responses against C. perfringens are poorly understood. The present study used an experimental NE model to characterize lymphoid immune responses in the caecal tonsils (CT), bursa of Fabricius, Harderian gland (HG) and spleen tissues of broiler chickens infected with four netB+ C. perfringens strains (CP1, CP5, CP18, and CP26), of which CP18 and CP26 strains also carried the tpeL gene. The gross and histopathological lesions in chickens revealed CP5 to be avirulent, while CP1, CP18, and CP26 strains were virulent with CP26 being “very virulent”. Gene expression analysis showed that, while the virulent strains induced a significantly upregulated expression of pro-inflammatory IL-1β gene in CT, the CP26-infected birds had significantly higher CT transcription of IFNγ and IL-6 pro-inflammatory genes compared to CP5-infected or uninfected chickens. Furthermore, CP26 infection also led to significantly increased bursal and HG expression of the anti-inflammatory/regulatory genes, IL-10 or TGFβ, compared to control, CP5 and CP1 groups. Additionally, the splenic pro- and anti-inflammatory transcriptional changes were observed only in the CP26-infected chickens. An antibody-mediated response, as characterized by increased IL-4 and/or IL-13 transcription and elevated IgM levels in birds infected with virulent strains, particularly in the CP26-infected group compared to uninfected controls, was also evident. Collectively, our findings suggest that lymphoid immune responses during NE in chickens are spatially regulated such that the inflammatory responses against C. perfringens depend on the virulence of the strain.

Heavy Metals Content in Broiler Chicken Tissues and Health Risk Assessment Posed to Consumers in Jazan Region, Saudi Arabia

Heavy Metals Content in Broiler Chicken Tissues and Health Risk Assessment Posed to Consumers in Jazan Region, Saudi Arabia

The effect of gamma-octalactone supplementation on the content of chemical elements in the muscles and liver of broiler chickens

Relevance. In recent years, a large number of new herbal preparations have appeared on the market, including gamma-octalactone. It is a naturally occurring compound isolated from Eucalyptus viminalis leaf extract. This compound has a wide range of therapeutic properties, which is associated with its antiinflammatory, antiseptic effect, stimulates appetite, increases the secretion of digestive enzymes and increases performance in animals.Materials and methods. The paper studied the features of the accumulation of chemical elements in the body tissues of broiler chickens when the compound gamma-octalactone was included in the diet. The studies were carried out on broiler chickens of the Arbor Acres cross at the age of 7–42 days. According to the scheme of the experiment, inthe diet of chickens of the 1st, 2nd and 3rd experimental groups was added gamma-octalactone at a dosages of 0.05, 0.1 and 0.2 ml/kg of b.w./day. The elemental composition of biosubstrates was studied using atomic emission and mass spectrometry.Results. It was found that the addition of gamma-octalactone contributed to the redistribution of chemical elements in the body tissues of the experimental poultry. In particular, the broilers of the experimental group that received gamma-octalactone at a dosage of 0.05 ml/kg of b.w./day had higher concentrations of B, Co, Ni, Se, V, Zn in the pectoral muscles. An analysis of the concentrations of the main essential elements in the thigh muscles of broiler chickens showed that feeding gamma-octalactone at a dosage of 0.05 ml/kg of b.w./day was accompanied by a significant increase in the content of Ca, Na, Fe, Si and Zn and a decrease in the levels of Cr, Cu and Ni relative to poultry of the control group. There was also a significant increase in the concentrations of Cu, Fe, Se, Zn, Mg, P in the liver of chickens of the experimental group, who received gamma-octalactone in the diet at a dosage of 0.1 ml/kg of l.w./day, against the background of a decrease in the concentrations of Si and Na.

Integrated transcriptome analysis for the hepatic and jejunal mucosa tissues of broiler chickens raised under heat stress conditions

BackgroundHeat stress (HS) is one of the most important threats for the current poultry industry. Therefore, many efforts have been made to ameliorate the adverse effect of HS on poultry production; however, physiological and molecular mechanisms pertaining to HS are still limited in poultry. Therefore, the objective of the current study was to investigate functional alterations based on individual and integrated transcriptomes in the liver and jejunal mucosa tissues of broiler chickens exposed to HS conditions.ResultsBroiler chickens exposed to HS showed decreased growth performance and increased corticosterone concentrations in the feather. In the transcriptome analysis, the number of differentially expressed genes (DEGs) were identified in the liver and jejunal mucosa by HS conditions. In the liver, genes related to amino acid oxidation, tryptophan metabolism, lipid metabolism, oxidative phosphorylation, and immune responses were altered by HS, which support the reason why heat-stressed poultry had decreased productive performance. In the jejunal mucosa, genes related to defense systems, glutathione metabolism, detoxification of xenobiotics, and immune responses were differently expressed by HS conditions. The integrated transcriptome analysis with DEGs found in the liver and jejunal mucosa showed a considerable connectivity between the core nodes in the constructed networks, which includes glutathione metabolism, xenobiotic metabolism, carbon metabolism, and several amino acid metabolisms.ConclusionsThe core network analysis may indicate that increased requirement of energy and amino acids in the jejunal mucosa of broiler chickens exposed to HS conditions is likely compromised by increased oxidation and synthesis of amino acids in the liver. Therefore, our results may provide comprehensive insights for molecular and metabolic alterations of broiler chickens raised under HS conditions, which can aid in the development of the novel strategies to ameliorate the negative effect of HS on poultry productivity and health.

Efficacy of SDS For Protein Extraction from Broiler Muscles and Mammalian Liver Tissue

Background: The present study purports to check and validate the potential of sodium dodecyl sulfate (SDS) alone being a suitable and cost-effective lysis buffer for maximum and efficient protein extraction from various muscle tissues of broiler chicken and mammalian liver. Materials and Methods: Three different muscle tissues (I; chest, II; wing and III; leg) were extracted from randomly selected commercial broilers (n=4) while mice (n=3) were dissected for the extraction of liver tissue samples. 1:1 ratio (w/v) of SDS; 10 and 1.0 &amp; 1.5% was used for muscles and liver tissues, respectively for its best time optimization for protein extraction. After incubation, respective tissues were homogenized followed by centrifugation. The supernatant was then processed for crude protein (CP) extraction by Bromocresol Green (BCG) method. Results: SDS (10%) achieved a maximum yield of CP after 1 hour of incubation. When checked the co-dependence of SDS-reagent on muscle-tissue type and time of incubation, tissue I (chest) was found to give maximum CP contents after 1 hour of incubation, tissue II (wing) extracted more CP after 3 hours while tissue III (leg) rendered equal amounts of CP after 1, 2 and 3 hours of incubation, respectively. From the mammalian liver tissue maximum yield of CP (6.9 g/dl), and albumin (ALB) (1.6 g/dl) was obtained with 1.5% of SDS. While the CP and albumin (Alb) content was not detected after homogenization with 1.0% SDS. Significance was checked at (P&lt; 0.05). Conclusion: It is concluded from the above findings that 10% SDS is the best lysis buffer concentration to extract crude protein from all the studied broiler muscle tissues while from mice liver samples we found 1.5% SDS lysis reagent seems good than 1.0%. Furthermore, this simple and cheapest procedure and ease of preparation this reagent may be suitable for extraction of important tissue protein fractions.

Toxic Effects of Norfloxacin on Cartilage Tissues of Broiler Chicken

Background: Norfloxacin is reported to have a wide distribution in the body. It penetrates well into tissues of the genito-urinary tract and crosses the placenta as well and it has relatively high concentrations when it gets into bile. After the withdrawal of enrofloxacin by the U.S. FDA for its use in poultry, the importance of norfloxacin is getting increased and already some veterinary formulations are introduced by authorized companies to the market. Besides, its wide usage for various bacterial infections, new and unrecognized toxicities have emerged; where the most important finding from the pre-clinical evaluation of flouroquinolone was arthropathogenic potential in young animals. This study was conducted to investigate the toxic effects of norfloxacin on the cartilaginous tissues of broiler chickens. Materials and Methods: The norfloxacin powder was obtained from Trichem laboratories in Bangalore. For better dissolving 1gr norfloxacin was first added to 0.25 ml of acetic acid and 2 ml of 50mmol/L acetate buffer maintained at pH 4.5 was added and mixed until the drug was completely dissolved. The experiment was carried out under hygienic conditions and standard management. One-week-old broiler chickens were procured from a reputed hatchery and divided into five groups; each containing six chickens. The study was conducted for 28 days. Findings: In the result of the histopathological study, chondrocytes were swollen with degeneration, infiltrated neutrophils, and improper ossification. The chondrocytes degeneration around the blood vessels was also noticed. The lesions were supported by the biochemical finding of ALP (Alkaline phosphatase). On the 21st day of treatment. There was a significant increase in ALP values of groups IV, and V. On the 28th day of treatment the mean serum ALP was significantly increased in groups III, IV, and V birds (P &lt;0.05, P &lt;0.01, P &lt;0.01) respectively. They were compared to group I birds. Conclusion: It was concluded that norfloxacin has a toxic effect on the cartilaginous tissues of chickens at the dose of 333 and 1100 mg/kg orally.

Влияние кормовой микробной добавки на мясную продуктивность цыплят-бройлеров и качество мяса птицы

Abstract. The purpose of the research is to study the effect of a probiotic supplement on the productivity of broiler chickens of the fast-growing Cobb 500 cross and the quality of poultry meat. Research methods. To study the parameters of meat productivity of broiler chickens, slaughter and anatomical cutting of poultry carcasses were carried out, the morphological composition of individual parts of carcasses was evaluated, the chemical composition of poultry meat was analyzed by determining moisture (GOST 9793-74), fat (GOST 23042-86), protein (GOST 25011-81), the level of essential amino acids (capillary electrophoresis method), organoleptic parameters of poultry meat and broth were studied. Research results. It was found that the introduction of a fodder additive into the poultry diet contributes to the improvement of meat characteristics of the Cobb 500 cross, improves the quality properties of poultry meat, as well as the bio-value of poultry meat products. When studying meat productivity, it was revealed that in experimental groups the mass of the gutted carcass was higher than in the control by 1.45.3 %, the slaughter yield was higher by 0.31.4 %, the mass of all breast tissues of broiler chickens of experimental groups exceeded this indicator in the control by 1.78.2 %, the mass of all femoral tissues was higher by 2.07.8 %, and the mass of all shin tissues by 1.56.8 %. The results of the chemical analysis of muscles of broiler chickens showed that the protein content was higher in experimental groups compared to the control group by 0.180.60 %, with a decrease in fat by 0.060.16 %, and there was also a decrease in meat quality index in all experimental groups by 4.510.5 %. A positive statistically significant difference was revealed when analyzing the amino acid score in the muscles of birds of experimental groups compared with the control group. The organoleptic evaluation of boiled broiler chicken meat and broth from them in experimental groups showed superiority over the poultry meat of the control group and averaged 4.9 versus 4.8 points. Scientific novelty. For the first time, the meat productivity and meat quality of broiler chickens were evaluated when using a microbial additive based on autochthonous microorganisms of the genus Lactobacillus isolated from the contents of the gastrointestinal tract of wild quails in their diet.