Tissue Vibration Research Articles

Biomechanics of sound production in high-pitched classical singing

Voice production of humans and most mammals is governed by the MyoElastic-AeroDynamic (MEAD) principle, where an air stream is modulated by self-sustained vocal fold oscillation to generate audible air pressure fluctuations. An alternative mechanism is found in ultrasonic vocalizations of rodents, which are established by an aeroacoustic (AA) phenomenon without vibration of laryngeal tissue. Previously, some authors argued that high-pitched human vocalization is also produced by the AA principle. Here, we investigate the so-called “whistle register” voice production in nine professional female operatic sopranos singing a scale from C6 (≈ 1047 Hz) to G6 (≈ 1568 Hz). Super-high-speed videolaryngoscopy revealed vocal fold collision in all participants, with closed quotients from 30 to 73%. Computational modeling showed that the biomechanical requirements to produce such high-pitched voice would be an increased contraction of the cricothyroid muscle, vocal fold strain of about 50%, and high subglottal pressure. Our data suggest that high-pitched operatic soprano singing uses the MEAD mechanism. Consequently, the commonly used term “whistle register” does not reflect the physical principle of a whistle with regard to voice generation in high pitched classical singing.

Soft Tissue Conduction Activates the Auditory Pathway in the Brain.

Soft tissue conduction is a mode of hearing which differs from air and bone conduction since the soft tissues of the body convey the audio-frequency vibrations to the ear. It is elicited by inducing soft tissue vibrations with an external vibrator applied to sites on the body or by intrinsic vibrations resulting from vocalization or the heartbeat. However, the same external vibrator applied to the skin sites also excites cutaneous mechanoreceptors, and attempts have been made to assist patients with hearing loss by audio-tactile substitution. The present study was conducted to assess the contribution of the auditory nerve and brainstem pathways to soft tissue conduction hearing. The study involved 20 normal hearing students, equipped with ear plugs to reduce the possibility of their response to air-conducted sounds produced by the external vibrator. Pure tone audiograms and speech reception (recognition) thresholds were determined in response to the delivery of the stimuli by a clinical bone vibrator applied to the cheek, neck and shoulder. Pure tone and speech recognition thresholds were obtained; the participants were able to repeat the words they heard by soft tissue conduction, confirming that the auditory pathways in the brain had been stimulated, with minimal involvement of the somatosensory pathways.

Regional differences in cochlear nonlinearity across the basal organ of Corti of gerbil: Regional differences in cochlear nonlinearity

Auditory sensation is based in nanoscale vibration of the sensory tissue of the cochlea, the organ of Corti complex (OCC). Motion within the OCC is now observable due to optical coherence tomography. In a previous study (Cooper et al., 2018), the region that includes the electro-motile outer hair cells (OHC) and Deiters cells (DC) was observed to move with larger amplitude than the basilar membrane (BM) and surrounding regions and was termed the "hotspot." In addition to this quantitative distinction, the hotspot moved qualitatively differently than the BM, in that its motion scaled nonlinearly with stimulus level at all frequencies, evincing sub-BF activity. Sub-BF activity enhances non-BF motion; thus the frequency tuning of the OHC/DC region was reduced relative to the BM. In this work we further explore the motion of the gerbil basal OCC and find that regions that lack significant sub-BF activity include the BM, the medial and lateral OCC, and the reticular lamina (RL) region. The observation that the RL region does not move actively sub-BF (already observed in Cho and Puria 2022), suggests that hair cell stereocilia are not exposed to sub-BF activity in the cochlear base. The observation that the lateral and RL regions move approximately linearly sub-BF indicates that linear forces dominate non-linear OHC-based forces on these components at sub-BF frequencies. A complex difference analysis was performed to reveal the internal motion of the OHC/DC region and showed that amplitude structure and phase shifts in the directly measured OHC/DC motion emerge due to the internal OHC/DC motion destructively interfering with BM motion.

The Art of Diagnosis from Breath Sound: A Literature Review

Breath sounds provide relevant information related to lung abnormalities. It is often difficult to differentiate between breath sounds, this is due to the different characteristics of each breath sound. Differentiating the types of breath sounds is crucial in making an accurate diagnosis. Breath sounds are divided into normal breath sounds and abnormal (additional) breath sounds. Normal breath sounds are sounds that originate from the chest wall, such as tracheal, bronchial, bronchovesicular and vesicular breath sounds. Meanwhile, abnormal (additional) breath sounds are breath sounds that indicate an abnormal condition in the respiratory system. Normal and abnormal breath sounds have different characteristics such as intensity, duration, frequency, quality of air flow, air flow pattern, air distribution, body position, location of sound production, changes in pressure and vibration of dense tissue in the lungs.

Treadmill Deck Performance Optimization Design Based on Muscle Activity during Running

In previous research on treadmills, the main focus has been on comparing the physiological differences induced by running on treadmill decks and other exercise surfaces, with relatively little research on the mechanical properties of treadmill decks. Reducing sports injuries is a common desire of runners, which may be closely related to muscle activity. Obviously, the mechanical properties of the treadmill play an important role in this process. Muscle activity was evaluated based on a mass-spring-damper (MSD) model that provides a simulated signal of the ground reaction forces (GRF) and vibration of the lower-limb soft tissues (LLST) during the landing of the human body during running. We improved the original human motion model by considering the stiffness and damping effect of the treadmill deck. In addition, based on the theory of muscle activity regulation, the dimensionless objective function is established, and the particle swarm optimization algorithm is used to find the best range of treadmill deck parameters under pre- and post-fatigue conditions. The results show that the hardness of the treadmill deck can affect the regulation of muscle activity. Based on this, the parameters of the specific safe area of the treadmill deck are obtained, and the size of the safe area after fatigue is significantly reduced compared to that before fatigue. By studying the physiological effects of the mechanical properties of the treadmill deck on runners, the research results are expected to provide references for the design of treadmill deck parameters and reduce the risk of runners’ sports injuries, which has practical application value for treadmill design and runners’ health.

The influence of midsole horizontal and vertical deformation on soft tissue vibrations and bone acceleration during running

ABSTRACT Increased midsole deformation can limit exposure to high impact and vibration magnitudes while running. The aim of this study was to evaluate the effect of shoes eliciting different midsole deformation on ground reaction forces, heel impact, soft tissue vibrations and bone vibrations. Forty-eight runners performed a 5-min running task on an instrumented treadmill at a self-selected pace with four different shoes. Midsole horizontal and vertical deformations were quantified with relative displacement of seven reflective markers placed on the midsole of the shoe and tracked by eight optoelectronic cameras. Heel impacts, soft tissue and bone vibrations of lower leg muscle groups, sacrum and head were quantified with tri-axial accelerometers. Continuous wavelet transform was used to assess magnitude and frequency of the acceleration data. Linear mixed models and non-parametric one-dimensional regressions between the accelerometer data and shoe deformation were performed. Greater horizontal and vertical deformations decreased the magnitude (up to 4.6% per mm) and frequency (up to 0.6 Hz per mm) of soft tissue vibrations and bone accelerations. Accelerations of the heel, tibia, gastrocnemius medialis and vastus lateralis were more influenced than the sacrum and head. Increasing midsole deformation could therefore mitigate the risk of injury, while increasing running comfort and smoothness.

Toothed whales use distinct vocal registers for echolocation and communication.

Echolocating toothed whales (odontocetes) capture fast-moving prey in dark marine environments, which critically depends on their ability to generate powerful, ultrasonic clicks. How their supposedly air-driven sound source can produce biosonar clicks at depths of >1000 meters, while also producing rich vocal repertoires to mediate complex social communication, remains unknown. We show that odontocetes possess a sound production system based on air driven through nasal passages that is functionally analogous to laryngeal and syringeal sound production. Tissue vibration in different registers produces distinct echolocation and communication signals across all major odontocete clades, and thus provides a physiological basis for classifying their vocal repertoires. The vocal fry register is used by species from porpoises to sperm whales for generating powerful, highly air-efficient echolocation clicks.

Simultaneous acquisition of vibration and diffusion by multifrequency diffusion tensor imaging-magnetic resonance elastography (mDTI-MRE)

Magnetic Resonance Elastography (MRE) and Diffusion Tensor Imaging (DTI) are MRI techniques that provide complementary diagnostic information and utilize motion-sensitive magnetic field gradients. In MRE, tissue vibrations are encoded into the MRI signal phase, while in DTI the diffusive motion of water molecules is assessed by means of the MRI signal magnitude. Careful consideration of the motion-encoding gradient timing conditions and the mechanical frequencies allows the simultaneous acquisition of vibration and diffusion data without observable mutual interferences. We propose a multifrequency Diffusion Tensor Imaging-Magnetic Resonance Elastography (mDTI-MRE) protocol that measures the diffusive behavior along 24 directions and the full 3D displacement field at two mechanical frequencies. Using this method, mDTI-MRE provides information about the mechanical wave field and about a preferred direction, which may facilitate the accurate mechanical characterization of anisotropic biological tissues, such as brain white matter and skeletal muscle.

Reliability of Soft Tissue Vibration Measurement and Number of Steps Demanded during Treadmill Running.

The present study aims to determine the test-retest reliability of the input signal (INPUT) of foot impact and soft tissue vibration (STV) of the lower limb muscles during treadmill running. Twenty-six recreational runners participated in three running trials at constant velocity (10 km/h) within two days. The INPUT and STV of gastrocnemius medialis (GAS) and vastus lateralis (VL) were extracted from 100 steps measured by three triaxial accelerometers. The Intraclass Correlation Coefficient (ICC) was calculated to determine the Intra-trial and Inter-day reliability of the different variables. Intra-trial reliability results indicated that most of the INPUT and GAS STV parameters, except for damping coefficient and setting time, have good to excellent reliability (0.75 < ICC < 0.9) from the beginning of the run (10 steps) to the end. In contrast, only 4 VL STV parameters showed good reliability. Furthermore, inter-trial reliability measured on day one showed that the number of reliable parameters reduced, especially for VL STV, and more steps were required (20 < steps < 80) to achieve good reliability. Inter-day reliability results showed that only one VL STV parameter reached good reliability. Therefore, the present results show that the measurement of the foot impact and the calf muscle vibrations present a good to excellent reliability measured on a single trial and two trials carried out on the same day. The reliability of these parameters remains good when comparing two days of experimentation. We recommend measuring impact and STV parameters during treadmill running in the same session.

Does neuromuscular fatigue generated by trail running modify foot-ground impact and soft tissue vibrations?

ABSTRACT The purpose of the study was to assess the influence of a preceding mountain ultramarathon on the impact between the foot and the ground and the resulting soft tissue vibrations (STV). Two sessions of measurements were performed on 52 trail runners, before and just after mountain trail running races of various distances (from 40 to 171 km). Triaxial accelerometers were used to quantify the foot-ground impact (FGI) and STV of both gastrocnemius medialis (GAS) and vastus lateralis (VL) muscles during level treadmill running at 10 km·h−1. A continuous wavelet transform was used to analyze the acceleration signals in the time–frequency domain, and the maps of coefficients as well as the frequency and damping properties of STV were computed. Fatigue was assessed from isometric maximal voluntary contraction force loss of knee extensors (KE) and plantar flexors (PF) after each race. Statistical nonParametric Mapping and linear mixed models were used to compare the means between the data obtained before and after the races. FGI amplitude and GAS STV were not modified after the race, while VL STV amplitude, frequency and damping significantly decreased whatever the running distance. A significant force loss was observed for the PF (26 ± 14%) and KE (27 ± 16%), but this was not correlated to the changes observed in STV. These results might reveal a protection mechanism of the muscles, indicating that biomechanical and/or physiological adaptations may occur in mountain ultramarathons to limit STV and muscle damage of knee extensors. Trial registration: ClinicalTrials.gov identifier: NCT04025138..

Intra-cycle analysis of muscle vibration during cycling

ABSTRACT Cyclists are exposed for a long period to continuous vibrations. When a muscle is exposed to vibration, its efficiency decreases, the onset of fatigue occurs sooner, and the comfort of the cyclist is reduced. This study characterised the vastus lateralis (VL) soft tissue vibrations for different input frequencies and different pedalling phases. Ten cyclists were recruited to pedal at 55, 70, 85, and 100 rpm on a vibrating cycle ergometer that induced vibrations at frequencies ranging from 14.4 Hz (55 rpm) to 26.3 Hz (100 rpm). The VL vibration amplitude was quantified with a continuous wavelet transform and expressed as a function of the crank angle. The pedalling cycle was split into four phases (downstroke, backstroke, upstroke, and overstroke) to express the mean vibration amplitude and frequency of each phase. Statistical analysis depicted that VL vibration frequency increased with the pedalling cadence and that the VL was exposed to up to 50% more vibration amplitudes during the downstroke phase at a slow cadence. The increase in the pedal vibration frequency, a higher vibration transmission due to greater normal force on the pedal, and strong activation of the VL during the downstroke phase were discussed to explain these results.

The Viability of 3-D Power Doppler Imaging Using Continuous Mechanical Translation: Simulation and Theoretical Analysis.

Although conventional Doppler ultrasound is widely used for quantifying blood flow, it is restricted by its low sensitivity to detect slow flow. The incorporation of ultrafast ultrasound and spatial-temporal clutter filters can not only extensively boost the Doppler sensitivity to low-velocity slow flow but also facilitate the development of advanced 3-D Doppler techniques. In this work, we propose a novel 3-D Doppler method which extends 2-D imaging to 3-D through the continuous mechanical translation of a linear transducer. The viability of this method is assessed by simulations with the aids of a theoretical model. The combination of simulations and the theoretical model provides unique insights into the inherent mechanisms involved in the performance of this 3-D Doppler method and the roles of factors, such as tissue vibration characteristics, blood flow velocity, elevational point-spread-function profile, probe translating speed, and signal energy ratios.

Modal coupling of acoustic wave propagation and mechanical wall vibration in the time-varying vocal tract

Coupling between acoustic wave propagation and mechanical vibration of wall tissue along the vocal tract has long been considered to be an important loss mechanism in speech, affecting the frequencies and bandwidths of the lower formants. However, the detailed mechanism of energy transfer between fluid and structure as the shape of the vocal tract changes over time has not been fully elucidated. To examine this question, a time-domain finite-volume simulation of quasi-one-dimensional fluid flow and mechanical wall vibration was derived from underlying conservation laws. By calculating the eigenvalues and left and right eigenfunctions of the resulting implicit matrix recursion at each point in time, the sound field can be decomposed into modal vibrations describing coupled motion of air and tissue. Standing waves in the fluid within the vocal tract create sound waves that propagate in the wall tissue. Conversely, standing waves in the wall tissue excite waves in the fluid that propagate as sound. The coupling between airborne and mechanical modes of vibration and the transfer of energy between the two systems can easily be understood by examining the time-varying amplitude and phase of the spatial eigenfunctions. Illustrations are provided for simulations of VCV transitions.

Compression Garments Reduce Soft Tissue Vibrations and Muscle Activations during Drop Jumps: An Accelerometry Evaluation.

Objectives: To explore the effects of wearing compression garments on joint mechanics, soft tissue vibration and muscle activities during drop jumps. Methods: Twelve healthy male athletes were recruited to execute drop jumps from heights of 30, 45 and 60 cm whilst wearing compression shorts (CS) and control shorts (CON). Sagittal plane kinematics, ground reaction forces, accelerations of the quadriceps femoris (QF), hamstrings (HM) and shoe heel-cup, and electromyography images of the rectus femoris (RF) and biceps femoris (BF) were collected. Results: Compared with wearing CON, wearing CS significantly reduced the QF peak acceleration at 45 and 60 cm and the HM peak acceleration at 30 cm. Wearing CS significantly increased the damping coefficient for QF and HM at 60 cm compared with wearing CON. Moreover, the peak transmissibility when wearing CS was significantly lower than that when wearing CON for all soft tissue compartments and heights, except for QF at 30 cm. Wearing CS reduced the RF activity during the pre-, post-, and eccentric activations for all heights and concentric activations at 45 cm; it also reduced the BF activity during post- and eccentric activations at 30 and 60 cm, respectively. The hip and knee joint moments and power or jump height were unaffected by the garment type. Conclusion: Applying external compression can reduce soft tissue vibrations without compromising neuromuscular performance during strenuous physical activities that involve exposure to impact-induced vibrations.

Vocal Creativity in Elephant Sound Production.

Simple SummaryElephants are known for their complex vocalization system and for being able to imitate sounds. Here, we show that African elephants apply unusual and individualistic sound production mechanisms to generate idiosyncratic sounds. These sounds are produced by manipulating non-phonatory structures, e.g., applying an ingressive airflow at the trunk tip to emit extraordinarily high-frequency sounds or repeatedly contract superficial muscles at the trunk base to generate lower-frequency pulsated sounds. Intriguingly, each individual establishes its own distinctive sound-producing strategy (e.g., contracting different muscle bundles). The production of these sounds on cue is encouraged via positive reinforcement training. This suggests that social feedback and reinforcement can facilitate vocal creativity and learning behavior in elephants. Social interactions and positive feedback are also crucial for early speech learning in human infants. Increasing knowledge on sound production plasticity in elephants—long-living, highly social mammals—is crucial in the effort to better understand their communicative and vocal learning ability and its function in wild elephant populations.How do elephants achieve their enormous vocal flexibility when communicating, imitating or creating idiosyncratic sounds? The mechanisms that underpin this trait combine motoric abilities with vocal learning processes. We demonstrate the unusual production techniques used by five African savanna elephants to create idiosyncratic sounds, which they learn to produce on cue by positive reinforcement training. The elephants generate these sounds by applying nasal tissue vibration via an ingressive airflow at the trunk tip, or by contracting defined superficial muscles at the trunk base. While the production mechanisms of the individuals performing the same sound categories are similar, they do vary in fine-tuning, revealing that each individual has its own specific sound-producing strategy. This plasticity reflects the creative and cognitive abilities associated with ‘vocal’ learning processes. The fact that these sounds were reinforced and cue-stimulated suggests that social feedback and positive reinforcement can facilitate vocal creativity and vocal learning behavior in elephants. Revealing the mechanism and the capacity for vocal learning and sound creativity is fundamental to understanding the eloquence within the elephants’ communication system. This also helps to understand the evolution of human language and of open-ended vocal systems, which build upon similar cognitive processes.

Wearable RF Near-Field Cough Monitoring by Frequency-Time Deep Learning.

Coughing is a common symptom for many respiratory disorders, and can spread droplets of various sizes containing bacterial and viral pathogens. Mild coughs are usually overlooked in the early stage, not only because they are barely noticeable by the person and the people around, but also because the present recording method is not comfortable, private, or reliable for long-term monitoring. In this paper, a wearable radio-frequency (RF) sensor is presented to recognize the mild cough signal directly from the local trachea vibration characteristics, and can isolate interferences from nearby people. The sensor operates at the ultra-high-frequency band, and can couple the RF energy to the upper respiratory track by the near field of the sensing antenna. The retrieved tissue vibration caused by the cough airflow burst can then be analyzed by a convolutional neural network trained on the frequency-time spectra. The sensing antenna design is analyzed for performance improvement. During the human study of 5 participants over 100 minutes of prescribed routines, the overall recognition ratio is above 90% and the false positive ratio during other routines is below 2.09%.

How Is the Cochlea Activated in Response to Soft Tissue Auditory Stimulation in the Occluded Ear?

Soft tissue conduction is an additional mode of auditory stimulation which can be initiated either by applying an external vibrator to skin sites not overlying skull bone such as the neck (so it is not bone conduction) or by intrinsic body vibrations resulting, for example, from the heartbeat and vocalization. The soft tissue vibrations thereby induced are conducted by the soft tissues to all parts of the body, including the walls of the external auditory canal. In order for soft tissue conduction to elicit hearing, the soft tissue vibrations which are induced must penetrate into the cochlea in order to excite the inner ear hair cells and auditory nerve fibers. This final stage can be achieved either by an osseous bone conduction mechanism, or, more likely, by the occlusion effect: the vibrations of the walls of the occluded canal induce air pressures in the canal which drive the tympanic membrane and middle ear ossicles and activate the inner ear, acting by means of a more air conduction-like mechanism. In fact, when the clinician applies his stethoscope to the body surface of his patient in order to detect heart sounds or pulmonary air flow, he is detecting soft tissue vibrations.

A novel theory of Asian elephant high-frequency squeak production

BackgroundAnatomical and cognitive adaptations to overcome morpho-mechanical limitations of laryngeal sound production, where body size and the related vocal apparatus dimensions determine the fundamental frequency, increase vocal diversity across taxa. Elephants flexibly use laryngeal and trunk-based vocalizations to form a repertoire ranging from infrasonic rumbles to higher-pitched trumpets. Moreover, they are among the few evolutionarily distantly related animals (humans, pinnipeds, cetaceans, birds) capable of imitating species-atypical sounds. Yet, their vocal plasticity has so far not been related to functions within their natural communicative system, in part because not all call types have been systematically studied. Here, we reveal how Asian elephants (Elephas maximus) produce species-specific squeaks (F0 300–2300 Hz) by using acoustic camera recordings to visualize sound emission and examining this alongside acoustic, behavioral, and morphological data across seven captive groups.ResultsWe found that squeaks were emitted through the closed mouth in synchrony with cheek depression and retraction of the labial angles. The simultaneous emission of squeaks with nasal snorts (biphonation) in one individual confirmed that squeak production was independent of nasal passage involvement and this implicated oral sound production. The squeaks’ spectral structure is incongruent with laryngeal sound production and aerodynamic whistles, pointing to tissue vibration as the sound source. Anatomical considerations suggest that the longitudinal closed lips function as the vibrators. Acoustic and temporal parameters exhibit high intra- and inter-individual variability that enables individual but no call-subtype classification. Only 19 of 56 study subjects were recorded to squeak, mostly during alarming contexts and social arousal but some also on command.ConclusionOur results strongly suggest that Asian elephants force air from the small oral cavity through the tensed lips, inducing self-sustained lip vibration. Besides human brass players, lip buzzing is not described elsewhere in the animal kingdom. Given the complexity of the proposed mechanism, the surprising absence of squeaking in most of the unrelated subjects and the indication for volitional control, we hypothesize that squeak production involves social learning. Our study offers new insights into how vocal and cognitive flexibility enables mammals to overcome size-related limitations of laryngeal sound production. This flexibility enables Asian elephants to exploit a frequency range spanning seven octaves within their communicative system.

Previous hamstring muscle strain injury alters passive tissue stiffness and vibration sense

BackgroundHamstring strain is one of the most common among sports injuries. A previous history of this injury is considered a strong predictor of recurrent hamstring strain injury. It has been suggested that fascial tissue alters after muscle strain injury. However, the association between previous hamstring strain injury and tissue stiffness and vibration sense detection has not been investigated. ObjectivesWe aimed to determine whether a previous history of hamstring strain injury affects tissue stiffness and vibration sense in professional soccer players. MethodThe stiffness (MyotonPRO®) and vibration disappearance threshold (tuning fork) were measured in eight professional soccer players with previous history of hamstring strain and eight uninjured players. The differences between two groups’ means were analyzed. Side-to-side differences between injured and uninjured legs were also analyzed. Results: The tissue stiffness was higher, and the vibration disappearance threshold was lower, in previously injured players when compared to uninjured players. Similar differences were found between injured and uninjured legs. No significant relationship was detected between the age or body mass index (BMI) for both tissue stiffness and vibration disappearance threshold (all P < 0.05). ConclusionsSoccer players with a previous history of hamstring strain injury exhibited higher tissue stiffness and lower vibration sensitivity in the injured leg, regardless of the age and BMI. The results that players who have a previous hamstring strain injury with altered tissue stiffness and vibration sense will be useful and feasible evaluation for chronic muscle strain condition.

Automated quantitative analysis of vocal fold vibration using two-dimensional scanning videokymography after transoral laser microsurgery

Purpose The purpose of this study was to develop a quantitative automated analysis system for two-dimensional videokymography (2D VKG) using the threshold segmentation method, and to analyze the vocal fold vibration characteristics of vocally healthy subjects and patients who underwent CO2 transoral laser microsurgery using this system, and to examine in accuracy differences between automatic and automated analysis methods. Methods 29 male patients who underwent CO2 transoral laser microsurgery and 10 vocally healthy male participated in the study. Quantitative analysis such as opening quotient (OQ), phase symmetry index (PSI), amplitude symmetry index (ASI) of 2D VKG images was performed using an automated analysis program in which glottal area extraction by the threshold segmentation method and edge detection using a manual plotting technique were mixed. Results Automatic analysis enabled accurate quantitative analysis in 76.9% (30/39) of the total image. The automated analysis corrected by the manual plotting technique on the sample showing gross error showed higher accuracy than the automatic analysis. The results of automated and automatic analysis were not statistically different in ASI, but there were differences in OQ and PSI. In addition, vocally healthy group and patients group showed statistical significance in all parameters (OQ, ASI, PSI). Conclusions The 2D VKG, which can evaluate the vibration of the entire vocal fold tissue in real time, can be usefully utilized as a laryngeal imaging technique that visualizes the structure and function of the vocal fold. Quantitative analysis using automated and automatic analysis can increase the clinical usability of 2D VKG. Keywords: 2D VKG; Quantitative analysis; Automated analysis; CO2 transoral laser microsurgery; Segmentation