Tissues Of Treatment Groups Research Articles

Effect of moringa flower extract on liver function and histopathology appearance of obesity model rat's liver

This study aims to test and analyze the effectiveness of moringa flower extract on the liver function of male wistar strain white rats obesity model and how the histopathological picture. This research is a type of experimental quantitative research using post-test only group design. This study used test animals in the form of male white wistar rats with a body weight of 200-300 gr aged 2-3 months. The test animals were divided into 4 groups, the control group was only given regular food and distilled water, the treatment group was given high-fat food and Moringa flower extract at different doses, namely 200 mg / kgBB, 400 mg / kgBB, and 600 mg / kgBB. The results of the phytochemical test showed that moringa flower extract contains secondary metabolites in the form of saponins, tannins, flavonoids, alkaloids, and steroids. The administration of moringa flower extract at a dose of 600 mg/KgBB is effective in improving liver function in obese Wistar white rats. This improvement can be seen through the levels of ALT, AST, and histological structure of the liver that has improved. The results of histopathological observations of liver tissue in treatment group 3, namely moringa flower extract at a dose of 600 mg/KgBB, experienced the most significant improvement and approached the control group compared to other groups.

Role and mechanism of IL-6/STAT3/Th17 signaling pathway in the intervention of forsythia in acute lung injury of septic rats

Objective: To investigate the mechanism of interleukin-6 (IL-6) /signal transducers and activators of transcriptional 3 (STAT3) /helper T cell 17 (Th17) signaling pathway in lung injury of rats with sepsis intervened by forsythia, with a view to provide experimental basis for the role and mechanism of forsythia in the treatment of sepsis. Methods: In July 2021, 30 healthy Wistar male rats were selected and randomly divided into sham operation group, model group and treatment group, with 10 rats in each group. The rat model of sepsis was established by cecal ligation and puncture. 2 h after recovery, the rats were given traditional Chinese medicine forsythia orally, twice a day at an interval of 12 h. The wet/dry mass ratio (W/D) of lung tissue was detected 24 h after surgery. The morphological changes of lung tissue were detected by HE staining. Flow cytometry was used to detect Th17 population in peripheral blood. The expression levels of IL-6 and interleukin-17A (IL-17A) in serum were determined by enzyme-linked immunosorbent assay. The mRNA expression levels of IL-17A and IL-6 in lung tissues were detected by reverse transcription-polymerase chain reaction. The expression levels of STAT3 and IL-17A in lung tissue were determined by Western blotting. Results: Compared with model group, the W/D of lung tissue in treatment group was decreased, and the difference was statistically significant (P<0.05). HE staining of lung tissue showed that compared with the model group, the degree of lung lesion and injury was reduced in the treatment group. Compared with sham operation group, the proportion of Th17 cells in CD4 lymphocytes in peripheral blood of rats in model group was significantly increased (P<0.05). Compared with model group, the proportion of Th17 cells in CD4 lymphocytes in peripheral blood of rats in treatment group was significantly decreased (P<0.05). Compared with sham operation group, peripheral blood serum IL-6 and IL-17 of rats in model group were significantly increased (P<0.05). Compared with model group, IL-6 and IL-17 in peripheral blood serum of rats in treatment group were decreased (P<0.05). Compared with sham operation group, the expressions of IL-17A and IL-6 mRNA in lung tissue of model group were significantly increased (P<0.05). Compared with model group, the expressions of IL-17A and IL-6 mRNA in lung tissue of rats in treatment group were significantly decreased (P<0.05). Compared with sham operation group, the protein expressions of STAT3 and IL-17A in lung tissue in model group were significantly increased (P<0.05). Compared with model group, the pritein expressions of STAT3 and IL-17A in lung tissue in treatment group were significantly decreased (P<0.05) . Conclusion: Forsythia plays a role in alleviating lung injury by down-regulating the expressions of the signaling pathway IL-6/STAT3/Th17, which providing a new target for the treatment of sepsis induced lung injury.

Efficacy of apolipoprotein 2-Jifusheng in the treatment of osteomyelitis in rabbits

To evaluate the effect of one-stage treatment of bone morphogenetic protein 2 combined with Jifusheng in the experimental model of osteomyelitis in rabbits. The model of chronic osteomyelitis of tibia was established in 30 3-month-old male New Zealand white rabbits with a body weight of (2.0±0.5) kg, and the model was verified 4 weeks after operation. Thirty rabbits with osteomyelitis were randomly divided into 3 groups with 10 rabbits in each group (n= 10). The model group did not do any treatment after focal debridement, treatment group A was implanted with musculosheng at the site of focal debridement and bone defect, and treatment group B was implanted with apolipoprotein 2-Jifusheng at the site of focal debridement and bone defect. The therapeuticeffect was evaluated by blood biochemical, microbiological, imaging, pathological and immunohistochemical examination 8 weeks after operation. At 4 weeks after operation, 30 rabbits with osteomyelitis were successfully validated. The results of serological examination showed that the hypersensitive C-reactive protein (CRP) and white blood cell count(WBC)in the model group were significantly higher than those in the blank group at 2 and 4 weeks after operation. Eight weeks after treatment, the detection of blood indexes showed that the white blood cell count (WBC)and hypersensitive C reactive protein (CRP)in treatment group A and treatment group B were significantly lower than those in the model group (P<0.05), but there was no significant difference between treatment group An and treatment group B (P>0.05). Eight weeks after treatment, the bone defect area was measured. The bone volume/total volume (BV/TV) and bone mineral density (BMD)in treatment group A and B were significantly higher than those in the model group (P<0.05), and the bone volume/total volume (BV/TV) and bone mineral density(BMD)in treatment group B were significantly higher than those in treatment group A (P<0.05), the repair of bone defect was almost complete, and the bone volume and bone mineral density increased significantly. At 8 weeks after treatment, the number of osteoblasts/bone tissue area (N.Ob/T.Ar) and the number of osteoblasts/bone tissue perimeter (N.Ob/B.Pm) in treatment group A and B were significantly higher than those in model group, and the N.Ob/T.Ar and N.Ob/B.Pm in treatment group B were significantly higher than those in treatment group A. The immunohistochemical staining of TNF- α and IL-6 around bone tissue in treatment group A and treatment group B was significantly less than that in model group, but there was no significant difference between treatment group B and treatment group A. The combined application of apolipoprotein 2-Jifusheng can promote bone repair and reduce the inflammation of the focus. it can treat rabbits with osteomyelitis in one stage, provide objective basis for the formulation of clinical treatment strategy of osteomyelitis and further promote clinical research.

Thermosensitive hydrogels as a controlled release system for alkannin to improve localized treatment of Candida vaginitis after external beam radiotherapy in vitro and in vivo.

External beam radiotherapy increases the risk of Candida vaginitis in cervical cancer patients, which brings a lot of insufferable influence to their life. Here, we explored the efficacy of alkannin in the treatment of Candida vaginitis after external beam radiotherapy. We exploit thermosensitive hydrogel-mediated alkannin as the topical formulation in a rat model established in our work. Periodic acid-Schiff of vaginas indicated little Candida albicans adhered to the vaginal tissue in treatment group. Additionally, hematoxylin and eosin stain revealed that inflammatory response of high dose alkannin was reduced. Above all, the animal model was first established in our work for the clinical desire. Our results suggested the promising application of alkannin for the disease with satisfying fungicidal activity and anti-inflammatory activity.

Effects and mechanisms of Rapamycin on renal interstitial fibrosis in rats

Objective To discuss the intervention effects and mechanisms for Rapamycin on renal interstitial fibrosis in rats. Methods A total of 72 Wistar male rats were randomly divided into 3 groups, normal group(n=24), model group(n=24), treatment group(n=24). The model group and treatment group received adenine 200 mg/kg daily, and the treatment group was also given Rapamycin 5 mg/(kg·d) at the 8th day, the normal group was just given the same amount of normal saline for 6 weeks.In the end of the 2nd, 4th and 6th week, 8 rats in each group were sacrificed respectively.The expression of hepatocyte growth factor(HGF), transforming growth factor-β(TGF-β)and neutrophils gelatinases related apolipoprotein(NGAL)in each group were observed.The software of image analysis system was used for semi-quantitative analysis. Results HE and Masson staining results showed that the renal tubular were progressive swelling, and changed with interstitial fibrosis, atrophy and even necrosis in model group from 2 weeks to 6 weeks.The pathological changes of kidney were more ease in the treatment group compared with those in model group.Immunohistochemical staining results showed that HGF expression levels of renal interstitial tissue in model group and treatment group at the 2nd week were significantly higher than those of normal group(P<0.05), and were significantly decreased at the 4th week and 6th week(P<0.05); HGF expression levels of renal interstitial tissue in treatment group were significantly higher than those in model group(P<0.05). NGAL expression levels of renal interstitial tissue in model group and treatment group at the 2nd week were significantly higher than those in normal group(P<0.05), and were significantly decreased at the 4th week and 6th week(P<0.05); NGAL expression levels of renal interstitial tissue in treatment group were significantly higher than those in model group(P<0.05). TGF-β expression levels of renal interstitial tissue in model group and treatment group at the 2nd, 4th, and 6th week were significantly higher than those in normal group(P<0.05), while TGF-β expression levels of renal interstitial tissue in treatment group were significantly lower than those in model group(P<0.05). Conclusion Rapamycin could improve the rat kidney tissue pathology, relieve renal tubular expansion, and slow progression of renal interstitial fibrosis, and has certain protective effect to the kidney. Key words: Rapamycin; Renal interstitial fibrosis; Intervention mechanism

Protective effect of fasudil hydrochloride against acute renal injury in septicopyemia rats.

To observe the protective effect of fasudil hydrochloride against acute renal injury in septicopyemia rats. A total of 60 Wister rats were included in the study and divided into control group (n=10), model group (n=25) and treatment group (n=25). Model group and treatment group received intraperitoneal injection of endotoxin (ET) to establish acute renal injury models while the control group only received daily intraperitoneal injection of normal saline 1mL. Five rats were taken out of model group and treatment group respectively at 1h (T1), 6h (T2), 12h (T3), 24h (T4) and 48h (T5), for intraperitoneal injection of ET 30mg/kg. Treatment group received intraperitoneal injection of fasudil hydrochloride 30mg/kg 1h before injection of ET. For three groups, 5mL blood samples were collected from postcava for determination of serum creatinine and urea nitrogen levels at different time points. Concentrations of serum tumor necrosis factor α and ET-1 were determined by using ELISA. The renal pathologic changes were observed under the microscope. Serum creatinine levels in both model group and treatment group were significantly higher than control group at T2-T5 (P<0.05) while the levels in treatment group were significantly lower than control group at T3-T5 (P<0.05). At T2-T5, blood urea nitrogen levels in model group and treatment group were significantly higher than control group (P<0.05) while the levels in treatment group were significantly lower than model group at T3-T5 (P<0.05). Concentrations of serum tumor necrosis factor α in model group and treatment group were significantly higher than control group at T1-T5 (P<0.05) while the levels in treatment group were significantly lower than model group at T1-T5 (P<0.05). Serum ET-1 concentrations in model group and treatment group were significantly higher than control group at T1-T5 (P<0.05) while the levels in treatment group at T1-T4 were significantly lower than model group (P<0.05). Rats in control group showed no swelling or hyperemia in kidney cells but normal structure and normally arranged renal tubular epithelial cells. Obvious injury was observed in model group at T3 and renal tubular epithelial cells in disorder and at swelling condition, hyperemia and angiectasis in glomerulus, degenerative opacities and vacuolar degeneration, and maximized injury were observed at T4. Injury in renal tissue in treatment group was significantly milder than model group. Fasudil hydrochloride has the significantly protective effect against acute renal injury in septicopyemia rats.