Tissue Conditioner Research Articles

Tissue response in a rat model of denture stomatitis treated with tissue conditioner containing antifungal complexed with β-cyclodextrin.

Introduction. Tissue conditioners modified with antifungals are a potential alternative to denture stomatitis (DS) treatment.Gap Statement. Information on tissue response to this treatment before its clinical application is lacking.Aim. This study aimed to evaluate the tissue response of a tissue conditioner modified with antifungals in a rat model of DS.Methodology. After DS induction for 4 days under antibiotic therapy, Wistar rats had their intraoral devices (IODs) relined with the tissue conditioner Softone without (Soft) or with the MICs against Candida albicans of nystatin (Nys) or chlorhexidine (Chx) complexed or not with β-cyclodextrin (Nys:βCD and Chx:βCD). Three controls were included: healthy rats [negative control (Nc)], rats using a sterile IOD [sterile device (Sd)] and rats with DS that did not receive treatment (DS). After 4 days of treatment, the palatal mucosa under the IODs underwent histological processing for morphohistopathological and histometric analyses, morphology of collagen fibres (birefringence), immunohistochemistry for the expression of cell proliferation (proliferating cell nuclear antigen) and cytokine (IL-1β).Results. The Nc and Sd groups were similar (P>0.05), displaying epithelial and connective tissues without any discernible changes in the parameters assessed. The DS and Soft groups exhibited pronounced epithelial alterations, cell proliferation and expression of the cytokine IL-1β. In groups treated with drug incorporation (Nys, Chx, Nys:βCD and Chx:βCD), all samples demonstrated a reduction in tissue inflammation or complete tissue recovery, with an epithelium compatible with health. For the immunohistochemical parameters, the Chx, Nys:βCD and Chx:βCD groups were comparable with Nc (P>0.05).Conclusion. The proposed treatment could be promising for DS, as it led to the tissue recovery of the palatal mucosa. Nevertheless, much lower concentrations of complexed antifungals were required to achieve a similar or higher degree of tissue response compared with uncomplexed drugs in a modified tissue conditioner formulation.

A Comparative Evaluation of Antifungal and Physical Properties When Nanoparticles Are Incorporated Into the Tissue Conditioner: An In Vitro Study.

Objective The objective of this in vitrostudy was to comparatively evaluate the antifungaland physical properties of tissue conditioner incorporated with nanoparticles (NPs) of different types and concentrations. Materials and methods A total of 198 tissue conditioner samples were used in this study. The samples were categorized into a control group, namely, tissue conditioner without NPs (Group 1), and test groups, namely, tissue conditioner incorporated with zinc oxide (ZnO) NPs (Group 2) andmagnesium oxide (MgO) NPs (Group 3). The antifungal properties and surface roughness of the samples were evaluated. The groups were further subdivided into seven subgroups: control (without NPs), 5% ZnO NPs, 10% ZnO NPs, 15% ZnO NPs, 3% MgO NPs, 5% MgO NPs, and 7% MgO NPs by weight. Surface roughness was measured using an optical profilometer,and antifungal activity was measured in terms of the diameter of the inhibition zone (DIZ) using the well diffusion method over seven days. Results The result showed that the 5%ZnO NPs subgroup had the lowest mean surface roughness, whereas the 15% ZnO NPs subgroup had the highest antifungal activity. Increasing the concentration of NPsincreased the antifungal property, and there was a steady decrease in DIZ from day one to day seven in all test groups. Conclusion Our results showed that the incorporation of various concentrations of ZnO and MgO NPs into tissue conditioner samples positively affected their physical and antifungal properties. The highest antifungal activity was found in the 15% ZnO NPs subgroup, and the lowest surface roughness was found in the 5% ZnO NPs subgroup.

Antifungal agents incorporated in denture base materials: ascoping review of the current evidence and technology prospecting.

The aim of this study was to summarize existing data and perform technological prospecting on the effect of incorporating antifungal agents into denture base materials in inhibiting Candida spp., as well as to explore the antimicrobial properties of these materials. A comprehensive electronic search was carried out in six major bibliographic databases (PubMed, Scopus, Embase, Cochrane Library, Web of Science and Lilacs) until February 2024. In addition, international patent databases were also examined. The search process, study and patent selection, data extraction and risk of bias assessment were carried out independently by researchers. The collected data underwent qualitative analysis. A total of 10 718 articles were identified in the searched databases, of which 40 documents were included for qualitative data analysis (articles: 31; patents: 9). The majority of the studies focused on investigating tissue conditioners (n = 14) and acrylic resins (n = 14). The primary antifungal agents studied were nystatin (n = 15) and fluconazole (n = 13). The most commonly utilized microbiological evaluation methodology was the agar diffusion test (n = 16), followed by the microdilution (n = 7) and biofilm formation assays (n = 7). All of the studies investigated the inhibitory effect of these materials against Candida species. The incorporation of antifungal agents into denture base materials has been extensively studied and has shown a significant inhibitory response against Candida spp. across various methodological assays.

Assessment of occlusion changes during laboratory phase of relining: An in vitro study.

Assessment of occlusion changes during laboratory phase of relining is essential to evaluate the occlusal discrepancies that could get incorporated in the denture with the use of different relining materials. Since the long term stability and functional success of the denture is heavily influenced by occlusion, an In-vitro study to assess these changes after relining is warranted. The aim of the study is to evaluate the changes in occlusion during laboratory phase of relining procedure. This is an in vitro study with a total of 30 specimen. A total of 30 maxillary standardized dentures were fabricated after mounting on a semi adjustable articulator. These samples will be divided into three groups based on the relining material used (Autopolymerizing resin, Heat-cure resin, Tissue conditioner). The vertical dimension, Centric contact points and eccentric contact points were measured before and after relining. The variables were tested to see if they had a normal distribution using the Shapiro-Wilk test. Parametric distribution was seen for ECP leading to further comparison using one way analysis of variance (ANOVA). Non-parametric distribution was found while testing the VD, CCP leading to adoption of Kruskal-wallis test for comparison of groups. Dunn Bonferroni test was done for VD since results were significant. The results of this in-vitro study showed statistically significant difference with respect to change in vertical dimension in all groups pre and post relining (P = 0.005). The centric contact points showed lesser variation in position when comparing the pre to the post relining phase with the use of autopolymerising resins, whereas heat cure resins and tissue conditioners showed statistically significant difference in the centric point contacts post relining. No statistically significant changes were seen in eccentric occlusion post relining in all groups. Tissue conditioners showed minimum mean changes in eccentric contacts. Within the limitations of this study, the use of autopolymerising resins depicted the most stable results with respect to occlusion, for relining of dentures.

Comparative Evaluation of the Antifungal Activity of Four Essential Oils Incorporated in Tissue Conditioner on Candida albicans: An In Vitro Study

Comparative Evaluation of the Antifungal Activity of Four Essential Oils Incorporated in Tissue Conditioner on Candida albicans: An In Vitro Study

Novel antimicrobial applications of copper oxide nanoparticles after combination with tissue conditioner used in complete prostheses

BackgroundTissue conditioners are used for treating and improving the tissues supporting complete dentures. On the other hand, recent advances in nanotechnology have revolutionized various fields of science, including dentistry. The present study aimed to investigate novel antimicrobial applications of copper oxide nanoparticle-based tissue conditioner used in complete prostheses.MethodsThe present experimental study included 126 tissue conditioner samples with different concentrations of copper oxide nanoparticles (20%, 10%, 5%, 2.5%, 1.25%, 0.625%, and 0% w/w). The samples were incubated with Enterococcus faecalis, Pseudomonas aeruginosa, and Candida albicans in 24-well plates for 24 h. Then, samples from the wells were re-incubated for 24 h, and the microorganisms were counted.ResultsThe culture media containing E. faecalis and P. aeruginosa showed significantly different growth between different nanoparticle concentrations following 24 h (P < 0.001), showing a reduction in bacterial growth with increased nanoparticle concentration. Both bacteria did not show any growth at the 20% concentration. However, C. albicans showed significant differences in growth between different nanoparticle concentrations following 48 h (P < 0.001), showing a reduction in growth with increased nanoparticle concentration. Also, the least growth was observed at the 20% concentration.ConclusionsIn conclusion, the CuO nanoparticles were prepared using a green synthesis methon in the suitable sizes. Moreover, the tissue conditioners containing CuO nanoparticles showed acceptable antimicrobial properties against E. faecalis, P. aeruginosa, and C. albicans.

Effectiveness of a tissue conditioner containing antifungals in a rat model of denture stomatitis.

This study investigated the effectiveness of a drug-modified tissue conditioner in an animal model of denture stomatitis. Wistar rats wore a Candida albicans-contaminated palatal device for 4 days. Next, nystatin (Nys) or chlorhexidine (Chx) were added to a tissue conditioner in their raw or β-cyclodextrin-complexed (βCD) forms at their minimum inhibitory concentrations. As controls, one group was not subjected to any procedure (NC), one group used sterile devices, one group had denture stomatitis but was not treated (DS), and another had the devices relined with the tissue conditioner without the addition of any drug (Soft). After 4 days of treatment, treatment effectiveness was assessed visually, histologically, and through CFU count, and myeloperoxidase (MPO) and N-acetylglucosaminidase (NAG) assays. Rats from the Soft, Nys, Nys:βCD, and Chx groups presented a significant decrease in the microbial load compared with the untreated group. Treatment groups showed lower MPO and NAG activity compared to the non-treated group. The addition of antifungals to a soft tissue conditioner can be a promising approach for denture stomatitis treatment.

Effectiveness of Citrus paradisi Extract, its Synergistic Effect and Cytotoxicity when Incorporated into Tissue Conditioners against Candida Species

Effectiveness of Citrus paradisi Extract, its Synergistic Effect and Cytotoxicity when Incorporated into Tissue Conditioners against Candida Species

Effect of Food-Simulating Liquids on the Leachability of Plasticizers from Dental Tissue Conditioners.

Tissue conditioners are composed of poly(ethyl methacrylate) (PEMA) powder and plasticizer/ethanol mix liquid. Butyl phthalyl butyl glycolate (BPBG) plasticizer is commonly used in tissue conditioners, but the main concern with phthalate plasticizer is its leachability and biocompatibility, especially the estrogenic activity and cytotoxicity of phthalate. Therefore, acetyl tributyl citrate (ATBC) plasticizer has been introduced and formulated as plasticizer in tissue conditioner; however its leachability characteristics are still unknown. Furthermore, the effect of foodsimulating liquids toward leachability of BPBG and ATBC plasticizers has not been documented. The objective of this study was to compare the effect of food-simulating liquids on the leachability of plasticizers and hardness of two experimental tissue conditioners containing BPBG and ATBC plasticizers. Ten experimental materials were prepared using PEMA polymer powder with 95% plasticizer (BPBG and ATBC) and 5% ethanol by volume, using powder to liquid ratio of 1.67:1, and the thickness was controlled at 3 mm. Shore A hardness value was measured after immersion in distilled water, artificial saliva, 25% ethanol/water mix, 3% citric acid, and coconut oil at 37°C. Measurements were taken at 2 hours and 1, 2, 3, 4, 7, 10, 14, 21, 28, 42, 56, and 84 days. Six readings were taken for each sample and hardness change was calculated and statistically analyzed using Wilcoxon signed-rank test. Increase in hardness value was noted for both plasticizers over time with the highest increase was when immersed in coconut oil. Shore A hardness value was significantly higher in ATBC after 84 days of immersion in all food-simulating liquids. The increase in hardness is due to plasticizer/ethanol leaching; however, as ethanol content was the same (5%), the hardness change is attributed to the leaching of plasticizers. Leaching of both plasticizers was highest in coconut oil indicating that tissue conditioners may have a shorter intraoral lifetime in patients eating high-fat diet.

“Antimicrobial properties of tissue conditioner modified with chitosan and green-synthesized silver nanoparticles: a promising approach for preventing denture stomatitis”

BackgroundChitosan is known to inhibit the growth of many bacteria and fungi. Tissue conditioners are commonly used to prevent bone destruction under dentures. However, over time, these materials can become a suitable substrate for microbial growth. One approach to improving dental materials is the use of nanoparticles. This study examined the antifungal properties of chitosan and green technique-synthesized silver nanoparticles in combination with tissue conditioners.MethodsTissue conditioner materials were mixed with chitosan and silver nanoparticles at concentrations of 0.097%, 0.19%, and 0.37%, along with 1.25 ppm Nystatin, and their antimicrobial properties against Candida albicans were investigated. The growth rate was measured after 24 h of incubation at 37 °C. Non-parametric tests, such as the Kruskal-Wallis H test and Mann-Whitney U test with Bonferroni correction, were used for data analysis after verifying that the groups did not have a normal distribution.ResultsCompared with the control and Nystatin groups, the Chitosan-silver groups showed a significant decrease in the number of CFUs of Candida albicans.ConclusionsThe combination of chitosan and silver nanoparticles with tissue conditioner materials is a promising alternative for preventing and treating denture stomatitis. These findings suggest that using very small amounts of nanoparticles in dental materials could effectively prevent microbial growth, which could improve the longevity and efficacy of dental prosthetics and materials.

Evaluation of the Strain under Compression and Surface Roughness of Tissue Conditioners Incorporated with Silver Nano Particles and Chlorhexidine: An In Vitro Study

Evaluation of the Strain under Compression and Surface Roughness of Tissue Conditioners Incorporated with Silver Nano Particles and Chlorhexidine: An In Vitro Study

Studying The Anti Candidal-Activity of Different Herbal Oils Incorporated into Tissue Conditioner: (A Comparative study)

This in vitro study was undertaken with the aim of testing the efficacy of the tissue conditioner mixed with four different commercially available herbal oils (Origanum oil, (Nigella sativa) Black seeds oil, Garlic oil, and Ginger oil) against Candida albicans. Control groups tested with antifungal test discs included fluconazole, flucytosine, and tissue conditioner discs (Acrosoft). The susceptibility test of plant extracts alone was also conducted. The combination groups tested were Acrosoft mixed with ginger oil, Acrosoft mixed with origanum oil, Acrosoft mixed with black seed oil, and Acrosoft with garlic oil. Test discs were completely embedded in the tissue conditioner mixed with plant extracts and gently placed on the agar plates. The plates were incubated at 35 °C for 72 hours. After incubation, inhibiting diameters of various groups were noted. There was complete resistance of Candida albicans to both fluconazole and flucytosine, and there was no inhibition zone observed regarding the susceptibility of ginger oil, origanum oil, black seed oil, and garlic oils. Similarly, there was no inhibition of Candida albicans observed in Acrosoft material. For the combination of tissue conditioner with plant extracts, results showed no inhibition in black seed oil and ginger oil combined with tissue conditioner. In contrast, the combination of garlic oil and origanum oil with tissue conditioner showed a zone of inhibition, and the inhibition diameters ranged from 5-9 mm.

Application of Zeolites and Zeolitic Imidazolate Frameworks in Dentistry-A Narrative Review.

Zeolites and zeolitic imidazolate frameworks (ZIFs) are crystalline aluminosilicates with porous structure, which are closely linked with nanomaterials. They are characterized by enhanced ion exchange capacity, physical-chemical stability, thermal stability and biocompatibility, making them a promising material for dental applications. This review aimed to provide an overview of the application of zeolites and ZIFs in dentistry. The common zeolite compounds for dental application include silver zeolite, zinc zeolite, calcium zeolite and strontium zeolite. The common ZIFs for dental application include ZIF-8 and ZIF-67. Zeolites and ZIFs have been employed in various areas of dentistry, such as restorative dentistry, endodontics, prosthodontics, implantology, periodontics, orthodontics and oral surgery. In restorative dentistry, zeolites and ZIFs are used as antimicrobial additives in dental adhesives and restorative materials. In endodontics, zeolites are used in root-end fillings, root canal irritants, root canal sealers and bone matrix scaffolds for peri-apical diseases. In prosthodontics, zeolites can be incorporated into denture bases, tissue conditioners, soft denture liners and dental prostheses. In implantology, zeolites and ZIFs are applied in dental implants, bone graft materials, bone adhesive hydrogels, drug delivery systems and electrospinning. In periodontics, zeolites can be applied as antibacterial agents for deep periodontal pockets, while ZIFs can be embedded in guided tissue regeneration membranes and guided bone regeneration membranes. In orthodontics, zeolites can be applied in orthodontic appliances. Additionally, for oral surgery, zeolites can be used in oral cancer diagnostic marker membranes, maxillofacial prosthesis silicone elastomer and tooth extraction medicines, while ZIFs can be incorporated to osteogenic glue or used as a carrier for antitumour drugs. In summary, zeolites have a broad application in dentistry and are receiving more attention from clinicians and researchers.

Comparison of Mechanical Properties of Three Tissue Conditioners: An Evaluation In Vitro Study.

Introduction: Tissue conditioners have been widely used in various clinical applications in dentistry, such as treating inflamed alveolar ridges, temporarily relining partial and complete dentures, and the acquisition of functional impressions for denture fabrication. This study aimed to investigate the mechanical properties of the most prevalent tissue conditioner materials on the market, including Tissue Conditioners (TC), Visco Gel (VG), and FITT (F). Materials and Methods: The three tissue conditioners, TC, VG, and F, were assessed based on the parameters mentioned above. The following tests were performed based on the ISO 10139-1 and ISO 10139-2 requirements: Shore A hardness, denture plate adhesion, sorption, water solubility, and contraction after 1 and 3 days in water. Additional tests are described in the literature, such as ethanol content and gelling time. The tests were carried out by storing the materials in water at 37 °C for 7 days. Results: The gel times of all tested materials exceeded 5 min (TC = 300 [s], VG = 350 [s]). In vitro, phthalate-free materials exhibited higher dissolution in water after 14 days (VG = -260.78 ± 11.31 µg/mm2) compared to F (-76.12 ± 7.11 µg/mm2) and experienced faster hardening when stored in distilled water (F = 33.4 ± 0.30 Sh. A, VG = 59.2 ± 0.60 Sh. A). They also showed greater contractions. The connection of all materials to the prosthesis plate was consistent at 0.11 MPa. The highest counterbalance after 3 days was observed in TC = 3.53 ± 1.12%. Conclusions: Materials containing plasticizers that are not phthalates have worse mechanical properties than products containing these substances. Since phthalates are not allowed to be used indefinitely in medical devices, additional research is necessary, especially in vivo, to develop safe materials with superior functional properties to newer-generation alternatives. In vitro results often do not agree fully with those of in vivo outcomes.

Treatment of Denture Stomatitis Using Modified Tissue Conditioners: A Systematic Review.

The most common sequela of wearing removable dentures is denture stomatitis. This review article uses a Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) format to collect data regarding articles that report on the treatment of denture stomatitis using tissue conditioners modified with antifungal drugs, inorganic compound, and phytomedicines. Their advantages, disadvantages, and mechanism of action are discussed.

Long-Term Antibacterial Efficacy of Cetylpyridinium Chloride-Montmorillonite Containing PMMA Resin Cement.

Despite being able to adhesively restore teeth, adhesives and cement do not possess any anticariogenic protection potential, by which caries recurrence may still occur and reduce the clinical lifetime of adhesive restorations. Several antibacterial agents have been incorporated into dental adhesives and cement to render them anticariogenic. Due to an additional therapeutic effect, such materials are classified as 'dental combination products' with more strict market regulations. We incorporated cetylpyridinium chloride (CPC), often used for oral hygiene applications, into montmorillonite (CPC-Mont), the latter to serve as a carrier for controlled CPC release. CPC-Mont incorporated into tissue conditioner has been approved by the Pharmaceuticals and Medical Devices Agency (PmontMDA) in Japan. To produce a clinically effective dental cement with the antibacterial potential to prevent secondary caries, we incorporated CPC-Mont into PMMA resin cement. We measured the flexural strength, shear bond strength onto dentin, CPC release, and the biofilm-inhibition potential of the experimental CPC-Mont-containing PMMA cement. An 8 and 10 wt% CPC-Mont concentration revealed the antibacterial potential without reducing the mechanical properties of the PMMA cement.

Effect of non-thermal oxygen-DBD plasma treatment on reducing the phthalate leach and in improving the mechanical strength properties of polymethyl methacrylate denture base and denture liner

Phthalates and esters of aromatic carboxylic acids are used as plasticizers in certain denture soft-lining materials and tissue conditioners. The presence of phthalates is of concern, because they may induce undesirable biological effects such as hormonal disorders, reproductive toxicity, hepatocellular tumours, genital disorders owing to a capacity to bind oestrogen receptors. Despite the potential risk posed by phthalates, their use in denture and denture liner is unavoidable due to the lack of efficient substituent material. Varied curing cycles, solvent wash, thermal plasma treatment/curing, etc., are some of the methods used to eliminate the phthalate content. The use of varied curing cycles, thermal plasma curing etc., will harm the polymer matrix of the acrylic denture resulting in further complication of the acrylic-liner interface bond architecture such as increased wettability, formation of pores, formation of gas pockets, micro fractures at the edges, etc. Hence, in order to overcome the above mentioned, curing of the materials under non thermal Oxygen dielectric barrier discharge (NT-O2/DBD) plasma is suggested. Sets of research samples prepared and lined with PDMS liners (as per ISO 178) were treated with NT-O2/DBD plasma. The treated samples were subjected to mechanical strength tests and calorimetric phthalate leach test. The results indicated that NT-O2/DBD plasma reduced the phthalate content remarkably and improved the overall mechanical strength properties when compared to the controls (thermal plasma treated and heat cured samples).

Antifungal Efficacy of Ocimum Basilicum Essential Oil in Tissue Conditioner Against Candida Albicans: An In vitro Study.

The antimicrobial activities were determined using disc diffusion, minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). After incorporation into tissue conditioner, the color difference and surface roughness were evaluated after one day. The surface morphology and the antibiofilm activity were assessed on day 1 and day 14. The zone of inhibition of OBEO on Sabouraud Dextrose agar was significantly wider than nystatin and deionized water (P=0.000) with the MIC and MFC values were 1.25% and 5%. Both OBEO concentrations incorporated into tissue conditioner, there was no significant difference of color changes in the light-dark character (P=0.102) and surface roughness (P=0.920) when compared to the control group. As for adherence at days 1 and 14, both concentrations of OBEO in tissue conditioner show significantly reduced adherence of C. albicans compared to the control group (P=0.000). OBEO exhibited significantly better antifungal activity against C. albicans when compared to nystatin and deionized water and resulted in no significant color changes, better surface roughness and lesser adherence of C. albicans toward the tissue conditioner.

Tıbbi Bitkilerin Doku Şartlandırıcılara İlave Edilmesiyle Antibakteriyel ve Antifungal Etkinlik Sağlanabilir Mi?

Bu çalışmanın amacı, antienflamatuar, antibakteriyel ve antioksidan özelliklerinden faydalanmak amacıyla tıbbi bitkilerin doku şartlandırıcılara ilave edilmesini konu alan literatürlerin taranması ve başarılı bir tedavi için kanıta dayalı bilgi verilmesidir. Bu çalışmada, diş hekimliğinde bitkilerin antimikrobiyal ve antifungal özelliklerinin değerlendirilmesi ve doku şartlandırıcılara ilave edildiğinde elde edilen sonuçların irdelenmesi amacıyla PubMed aracılığı ile Medline veri tabanında literatür taraması yapılmıştır. Araştırılan makaleler sonucunda, bitkilerin doku şartlandırıcılara ilavesinin diş hekimliğinde alternatif bir destekleyici tedavi seçeneği olabileceği tespit edilmiştir. Bununla birlikte, yapılan tüm değerlendirmelerden sonra, bu uygulamaların uzun dönem başarısı ve klinik kullanımları açısından daha ileri çalışmalara ihtiyaç duyulmaktadır.

Role of Nanotechnology in Prosthodontics: a review

In the present-day context, a considerable portion of the population faces the challenge of edentulism. Various treatment approaches, including both fixed and removable prostheses, are available for restoring missing teeth. Noteworthy progress has recentlybeen observed in the characteristics of commonly used dental materials, attributed to the emergence of nanotechnology. This technological advancement has significantly contributed to improving the outcomes of dental treatments. Nanotechnology represents amultidisciplinary domain that amalgamates principles from both the arts and sciences, involving the manipulation of structures and functional materials at a minuscule scale. This field leverages various physical and chemical methodologies to operate at dimensions where sizes of particles, typically ranging from 1 to 100 nanometers, give rise to what is termed as Nanoparticles. The integration of nanoparticles in dentistry, particularly within dental materials such as ceramics, acrylic resin, tissue conditioners, and denture adhesives, has exhibited a discernible positive impact on their inherent properties. This review predominantly delves into an exploration of diverse applications of nanoparticles within the realm of dentistry, with a specific emphasis ontheir role in prosthodontics.