Node localization and temporal synchronization, as two key parts of each self-organized and localization-aware wireless sensor network (WSN), have been a key topic for research and applications. The initial prototype of the sensor network is the same as that of the local area network. All nodes are connected by wires, and there is a central control node. All nodes transmit data to the central node point-to-point. With the development and progress of wireless communication technology, the current sensor network has developed into a WSN. Without a central node, all nodes can communicate with each other; so, it is natural to develop positioning technology in WSN. Node positioning in wireless sensor networks refers to the process in which sensor nodes determine the location information of other nodes in the network through a certain positioning technology based on the location information of a few known nodes in the network. The principle of positioning is purely geometric in mathematics. With the in-depth promotion of WSN in the application field, there are more and more requirements for high precision positioning, the higher the positioning accuracy, the higher the requirements for network time synchronization, and the problem of node clock synchronization and high precision positioning of the node can be studied together. Solving the problem of node clock synchronization can further provide support for node positioning in a variety of different environments. As a new ultrabroadband (UWB) carrier-free communication technology with nanoscale temporal resolution, it has been widely used in high-precision node positioning systems in recent years, UWB technology is the most advanced noncarrier wireless communication technology that uses bandwidths above 1 GHz and uses nonsine wave narrow pulses from nanoseconds to picoseconds to transmit data. Therefore, it occupies a very wide spectrum. UWB technology has the advantages of low system complexity, low transmit signal power spectrum density, insensitive to channel fading, and high positioning accuracy. It is especially suitable for high-speed wireless access in dense multipath places such as indoors, providing a technical basis for the engineering implementation of high-precision positioning algorithms. However, the current situation of Chinese track and field events has not kept pace with the development of Chinese competitive sports, and even the level of individual events has a gradual decline. Therefore, it is very meaningful to study the relevant biological factors that affect sprint performance. This article analyzes the related biological factors that affect the performance of the sprint, combines the knowledge of physiology to analyze the training methods that appear in the sprint from the physiological perspective, and analyzes the related biological factors that affect the performance of the sprint. This article chooses to divide them into men’s group (3 groups) and women’s group (3 groups), with 4 people in each group. Experiment proved that after the experiment, these are the following factors: the fatigue of the nervous system, the technical difference of sprinting, the change of muscle fiber enzyme activity, the order of muscle fiber cross-sectional area and muscle activation, and the recruitment of muscle fiber types. Impact P value less than 0.05, which shows that the factors that affect sprint performance are complex, and the biological factors that affect step length can be studied through anthropometry. The impact of step frequency on sports performance is very important.