Time-location Sampling Research Articles

Vaccine Hesitancy at Nine Community Sites Across the United States, Early in COVID-19 Vaccine Rollout.

Vaccine hesitancy has been a significant concern throughout the COVID-19 pandemic. Vaccine hesitancy can be attributed to lack of confidence in vaccines, complacency about the health threat, or lack of convenience of vaccination. To date, few studies have used methods designed to include populations underrepresented in research when identifying factors associated with vaccine hesitancy. Between January and July 2021, potential participants were recruited from community venues selected through time-location sampling in 15 defined communities in the United States. Study staff administered a questionnaire on demographics, COVID-19 behaviors and attitudes, and vaccination status or intention to consenting individuals. Vaccine hesitancy was analyzed among those age 18years and older from nine of the 15 sites and was defined as self-reported neutral, unlikely, or very unlikely vaccine intention. Logistic regression modeling, adjusted for site, identified factors associated with vaccine hesitancy. Among 11,559 individuals, vaccine hesitancy by site ranged from 8.7 to 31.1%. Vaccine hesitancy was associated with being Black compared to White, being White compared to Asian, younger age, unstable housing, being unemployed, lower income, having a disability, providing care in home, not reporting inability to visit sick or elderly relatives during the pandemic, not reporting increased anxiety during the pandemic, and not spending more time with loved ones during the pandemic. In these selected US communities, early in vaccine rollout, there were significant racial disparities in vaccine hesitancy. Additionally, individuals who were more marginalized due to their socioeconomic status were more likely to report vaccine hesitancy. Vaccine campaigns should make efforts to remove barriers to vaccination, by improving convenience.

The Development and the Assessment of Sampling Methods for Hard-to-Reach Populations in HIV Surveillance.

Due to stigma or legal issues, populations with higher HIV risk are often hard to reach, which impedes accurate population estimation of HIV burden. To better sample hard-to-reach populations (HTRPs) for HIV surveillance, various sampling methods have been designed and/or used since HIV epidemic following the first reported AIDS cases in 1981. This paper describes the development and the assessment (i.e., validity and reproducibility) of approximately eight sampling methods (e.g., convenience sampling, snowball sampling, time location sampling, and respondent-driven sampling) for HTRPs in HIV surveillance, with a focus on respondent-driven sampling (RDS). Compared to other methods, RDS has been greatly assessed. However, current evidence is still inadequate for RDS to be considered the best option for sampling HTRPs. The field must continue to assess RDS and to develop new sampling approaches or modifications to existing approaches.

The social-structural contexts of cannabis consumption and harm reduction among young Nigerian women

BackgroundYoung women remain under-represented in the research literature on young people's cannabis use. This study explored cannabis use and harm reduction practices of young Nigerian women. MethodsThe study draws on 19 in-depth interviews conducted with young women aged 21–35 years who were recruited through time-location sampling in Uyo, Nigeria. Interview transcripts were subjected to thematic coding and analysis. ResultsAccounts highlighted a rapid progression from occasional consumption during hang-outs to regular and heavy consumption for most participants. Frequent and heavy cannabis use, shaped by trauma, stress and mental health problems linked to participants’ marginal social locations, established a context of risk for health harms. The participants sought to reduce harms by attempting to reduce frequency and quantity of cannabis consumed, often with limited success. They also micro-dosed to monitor drug effects, avoided mixing cannabis with more potent drugs, sought out cannabis strains with lower THC as well as consuming privately to reduce social harms such as violence and police arrest associated with using in street milieus. On the other hand, cannabis consumption was seen as helping to reduce harms by providing a relatively safer and less addictive alternative to drugs such as heroin and pharmaceutical opioids, preventing high-risk sexual exchanges often associated with opioid withdrawal among women, and relieving pain. ConclusionsCannabis was consumed recreationally at first, and harmful consumption patterns developed from using cannabis to treat the psychological symptoms of structural inequalities. The analysis supports the social-structural production of drug-related harms, foregrounding the importance of structural-level interventions for creating an enabling environment for safer consumption.

Adaptive Time-Location Sampling for COMPASS: A SARS-CoV-2 Prevalence Study in Fifteen Diverse Communities in the United States.

COVID-19 has placed a disproportionate burden on underserved racial and ethnic groups, community members working in essential industries, those living in areas of high population density, and those reliant on in-person services such as transportation. The goal of this study was to estimate the cross-sectional prevalence of SARS-CoV-2 (active SARS-CoV-2 or prior SARS-CoV-2 infection) in children and adults attending public venues in 15 sociodemographically diverse communities in the United States and to develop a statistical design that could be rigorously implemented amidst unpredictable stay-at-home COVID-19 guidelines. We used time-location sampling with complex sampling involving stratification, clustering of units, and unequal probabilities of selection to recruit individuals from selected communities. We safely conducted informed consent, specimen collection, and face-to-face interviews outside of public venues immediately following recruitment. We developed an innovative sampling design that adapted to constraints such as closure of venues, changing infection hotspots, and uncertain policies. We updated both the sampling frame and the selection probabilities over time using information acquired from prior weeks. We created site-specific survey weights that adjusted sampling probabilities for nonresponse and calibrated to county-level margins on age and sex at birth. Although the study itself was specific to COVID-19, the strategies presented in this article could serve as a case study that can be adapted for performing population-level inferences in similar settings and could help inform rapid and effective responses to future global public health challenges.

Community stigma, victimization, and coping strategies among gay, bisexual, and other cis-gender men who have sex with men in slum communities in Ghana. BSGH-003

BackgroundGay, bisexual, and cis-gender men who have sex with men (GBMSM) face severe consequences, especially within stigmatized environments. However, very little is known about the experiences of GBMSM living in slums in SSA and Ghana. This study investigates the experiences of stigma, victimization, and coping strategies and proposes some interventional approaches for combating stigma facing GBMSM in slum communities.MethodsWe engaged GBMSM living in slums in two major Ghanaian cities. We used a time-location sampling and collected data through in-depth individual interviews. Two major themes emerged from the study: (1) insecurities and criminalization of GBMSM activity, and (2) GBMSM coping strategies.ResultsFindings show GBMSM experienced negative attitudes from the community due to their sexual behavior/orientation. GBMSM also developed coping strategies to avert negative experiences, such as hiding their identities/behavior, avoiding gender non-conforming men, and having relationships with persons outside their communities.ConclusionWe propose interventions such as HIV Education, Empathy, Empowerment, Acceptance, and Commitment Therapy as possible measures to improve the experiences of GBMSM living in Ghanaian slum communities.

Biobehavioral survey using time location sampling among female sex workers living in Ghana in 2020.

The HIV epidemic in Ghana is characterized as a mix of a low-level generalized epidemic with significant contributions from transmission among female sex workers (FSW) and their clients. This study seeks to identify and describe key characteristics and sexual behaviors of FSW and estimate the prevalence of HIV, syphilis, gonorrhea, chlamydia, and hepatitis B virus (HBV) among FSW in Ghana. A total of 7,000 FSW were recruited for the study using Time Location Sampling (TLS) approach with 5,990 (85.6%) participants completing both biological and the behavioral aspects of the study. A structured questionnaire was administered to respondents to assess several factors, such as background characteristics, sexual risk behaviors, condom usage, HIV/AIDS knowledge, opinions, and attitudes. Trained staff conducted face-to-face interviews using mobile data collection software (REDCap) after provision of specimens for HIV and STI testing. Descriptive statistics such as medians, ranges, charts, and percentages are performed and presented. Also included, are bivariate analyses to establish relationships between FSW type and other relevant characteristics of the study. Among the 7,000 (100%) FSW sampled from all regions, 6,773 took part in the behavioral and 6,217 the biological. There were 783 (11.2%) respondents who took part only in the behavioral and 227 (3.2%) only in the biological. Most were young, with a median age of 26 years, majority had never been married or were widowed/divorced and a quarter had no education or had only primary education. Majority (74.8%) of FSW first sold sex at age 25 years or less with a median age of 20 years. Most (84.8%) of the FSW indicated that they entered sex work for money, either for self or family and had an average of eleven (11) sexual partners per week. More than half (55.2%) of the FSW were new entrants who had been in sex work for less than 5 years before the study. Consistent condom use with paying clients was generally unsatisfactory (71%), and was however, very low (24%) with their intimate partners or boyfriends. Only about half (54.6%) of FSW have been exposed to HIV prevention services in the last three months preceding the survey, and this varies across regions. Overall, comprehensive knowledge about HIV and AIDS was low. Only 35% of FSW had comprehensive knowledge. HIV prevalence was 4.6% and was higher among seaters (brothel-based) and older FSW who had been sex work for a longer period. The HIV prevalence from the previous bio-behavioral survey (BBS) in 2015 and 2011 were estimated to be 6.9 and 11.1%, respectively. Compared to the results from the previous studies, the findings give an indication that Ghana is making significant progress in reducing the burden of HIV among FSW in the country. However, risky behaviors such as low consistent condom use, low coverage of HIV services across the regions, and low comprehensive knowledge could reverse the gains made so far. Immediate actions should be taken to expand coverage of HIV services to all locations. Efforts must be made to reach out to the new entrants while also addressing strongly held myths and misconceptions about HIV.

Interaction of social support and depressive symptoms on antiretroviral therapy adherence among people living with HIV in South Africa.

Depression consistently emerges as a significant predictor of poor antiretroviral therapy (ART) adherence among adult people living with human immunodeficiency virus (PLHIV). However, a gap exists regarding how social support and depressive symptoms can interact to influence ART adherence among adult PLHIV in South Africa (SA). To investigate the interaction between social support and depressive symptoms on ART adherence among adult PLHIV. A tertiary hospital in Durban, KwaZulu-Natal province of SA. Utilising a quantitative cross-sectional research design along with time location sampling technique (TLS); the study recruited 201 adult patients enrolled in an ART programme. The results indicated that depressive symptoms were significantly associated with ART adherence with and without the interaction (B = -0.105; odds ratios [OR] 0.901; 95% confidence intervals [CI] = 0.827, 0.981; p = 0.016), while social support was not significantly associated with ART adherence (B = 0.007; OR 1.007; 95%CI = 0.989, 1.025; p = 0.475). However, a statistically significant interaction was found between social support and depressive symptoms (B = -0.006; OR 0.994; 95%CI = 0.989, 1.000; p = 0.037) on ART adherence. Based on the results, depressive symptoms significantly influenced ART adherence. However, social support did not buffer the adverse effects of clinical depression associated with poor ART adherence. This study provides an evidence-based approach to address gaps in the mental health and social well-being of PLHIV in the context of ART adherence.

The Moderating Role of HIV Stigma on the Relationship between Perceived Social Support and Antiretroviral Therapy Adherence Self-Efficacy among Adult PLHIV in South Africa.

People living with human immune deficiency virus (PLHIV) grapple with distinct challenges, including HIV stigma which affects their antiretroviral therapy (ART) adherence self-efficacy. This study investigates the interaction of HIV stigma and perceived social support on ART adherence self-efficacy among adult PLHIV in South Africa. This study utilized a cross-sectional design that involved 201 participants selected using time location sampling at a tertiary health facility in Durban. HIV stigma was significantly and negatively associated with self-efficacy (β = -7.860, t = -4.654, p = .001), with variations across different stigma levels (β = -5.844, t = -4.003, p = .001). Social support was significantly and positively associated with self-efficacy at lower HIV stigma levels (β = 7.440, t = 3.887, p = .001), in contrast to higher levels (β = -2.825, t = 1.400, p = .163). Social support significantly influences ART adherence self-efficacy, particularly at lower levels of HIV stigma, but the effect of support weakens as stigma intensifies.

Correlates of prevalent HIV infection among adolescents, young adults, and older adult female sex workers in Ghana: Analysis of data from the Ghana biobehavioral survey.

Human immunodeficiency virus infection remains a high burden among key populations such as female sex workers in the world. We aimed to provide distribution of prevalence and correlates of Human immunodeficiency virus infection among adolescent, young, and older adult FSWs in Ghana. This data was obtained from the biobehavioral survey of female sex workers (2020) in Ghana based on a time location sampling approach for the selection of respondents. A sampling frame was developed taking into consideration list of venues, days, and time that sex workers operate across all the regions of Ghana. These lists were derived from a sampling universe which was obtained during a mapping exercise. All sex workers aged 16 years and above and eligible on the day of visit participated. Human immunodeficiency virus testing was done based on First Response and Oraquick. To obtain estimates for sex workers, sampling weights were calculated and applied to the dataset. Inferential analyses using Bayesian regression models were applied with interaction effects. A total of 5,990 participants completed both the biological and behavioral aspects of the study. The HIV prevalence among female sex workers in Ghana was 4.67% (CI: 4.05%, 5.40%). About 70% of the respondents who tested positive for Human immunodeficiency virus were among the older adults (= >25 years) group. Generally, there was a high prevalence variation across the 16 regions of Ghana, from 0.00% to 8.40%. Respondents' age was a significant contributor to the prevalence of HIV. Respondents who were forced into having sex had higher odds (38%) of being positive in the combined analysis. Respondents who had comprehensive knowledge of HIV had lower odds (39%) of testing positive. The findings suggest a low prevalence of HIV among sex workers in 2020 compared to the 2011 and 2015 biobehavioral survey results but higher than the general population. Specifically, older adults have a higher prevalence of HIV. There is generally low level of comprehensive knowledge among sex workers. Interventions geared towards increasing FSW knowledge on risky behavior should be vigorously pursued.

Kuwait's circular prototype mosque design: a history and comprehensive evaluation

PurposeThis study evaluates the architectural status of circular prototype mosques in Kuwait. The argument is that the once dominant and powerful image of mosques is now merely a repetitive reproduction through the prototype scheme. The study focuses on the circular prototype design, which has been constructed in many of Kuwait's recent residential areas. It evaluates qualitatively the worshipers' experiences of these mosques.Design/methodology/approachThe research methodology is qualitative. The main question is how well the circular prototype mosques are received by worshipers and local communities. Various research methods were used, including walk-through survey and group interview with worshipers and semi-structured interview with key informants in Public Authority for Housing Welfare and Ministry of Awqaf and Islamic Affairs. Purposive sampling was chosen to select for key informants. Time-location sampling was selected for worshipers' group interview.FindingsCircular prototype presents several weaknesses. The community did not receive the circular design well as it deviates from the traditional and modern rectangular mosque design prevalent in Kuwait. This research highlights the importance of considering traditional design principles, community preferences and functional requirements while designing a mosque. It also emphasizes the need for an extensive evaluation of prototype designs to identify potential weaknesses before proceeding with the final design.Social implicationsIt is recommended that future mosque design projects in Kuwait consider traditional design principles; community preferences; and financial, functional and sustainability requirements. In addition, the findings of this study can be used to inform future mosque design projects in Kuwait and to ensure that they are functional, cost-effective and well received by the community.Originality/valueThis research provides an informative and comprehensive analysis of Kuwait's prototype mosque designs from the 1950s to the present day. It focuses on the current circular prototype, critically examining its advantages and disadvantages. This research is the first to evaluate the history and design improvements over the years. As such, this research offers invaluable information to those interested in Kuwait's religious architecture and cultural history.

Life-time HIV testing among people who inject drugs in Iran: results from the National Rapid Assessment and Response survey.

HIV testing is recommended for people who inject drugs (PWID). The aim of this study was to determine the prevalence of lifetime HIV testing among PWID and to better understand the predictors for HIV testing in a convenience sample across Iran. This study is a secondary analysis of Iran's National Rapid Assessment and Response survey conducted between October 2016 and March 2017. Analysis includes the 999 people who reported injecting drugs across the capital cities of 21 provinces. Data were collected by using the venue-based application of the Time Location Sampling (TLS) frame. Chi-square tests were used to examine the difference between HIV testing across different quantitative variables. Logistic regression was used to determine the predictors of life-time HIV testing. Analysis was performed using STATA V.12 software with a significance level of 95%. Most participants were male (n = 902, 93.50%), and over half (n = 555, 59.17%) were older than 35 years old. About one-third, (n = 326, 38%) of people interviewed were single and another one-third (n = 251, 29%) reported being divorced. Over two-thirds of participants (n = 678, 69.78%) in this study reported lifetime HIV testing. The results from a multiple variable logistic regression showed people with a university education were more likely to have been previously tested for HIV than illiterate people (OR = 18.87, 95%CI 2.85-124.6, value of p = 0.002). Those individuals who reported ever receiving methadone treatment were 2.8 times more likely to have been tested for HIV than individuals without methadone treatment experience (OR = 2.89, 95%CI 1.53-5.42, value of p < 0.001). Needle syringe sharing in last month, was negatively associated with life-time HIV testing (OR = 0.29, 95%CI 0.17-0.48, value of p < 0.001). Despite Iran's wide availability and access to counseling services for HIV testing in key populations, the proportion of PWID being tested for HIV could be improved. Developing effective strategies to increase people's understanding and awareness of the importance of and need for HIV prevention and familiarity with HIV testing sites is an essential step in increasing HIV testing for this population. Studies on more recent HIV testing are required to better assess and understand the frequency of HIV testing among PWID in Iran.

Estimating prevalence and modelling correlates of HIV test positivity among female sex workers, men who have sex with men, people who inject drugs, transgender people and prison inmates in Sierra Leone, 2021

Key populations (KPs) are particularly vulnerable to HIV infection and efforts to prevent HIV infections among KPs have been less successful, largely due to existing laws and legislation that classify the groups as illegal. Understanding the HIV infection pathway and the burden of HIV infection among Female Sex Workers (FSWs), Transgender people (TG), Men who have sex with Men (MSM), People who Inject Drugs (PWID), and Prison Inmates (PIs) is critical to combatting the HIV epidemic globally. This study aims to estimate HIV prevalence and model the risk factors of HIV positivity rate among the aforementioned KPs in Sierra Leone. This study used Time Location Sampling, Respondent Driven Sampling (RDS), and Conventional cluster Sampling designs to generate a representative sample of FSWs, MSM, TG, PI, and PWID. HIV prevalence and the corresponding 95% confidence intervals among each KP were estimated by adjusting for sampling weight using the logit-transformed confidence intervals. To determine correlates of HIV test positivity among KPs, a multivariable modified Poisson regression model that adjusts for RDS survey weights was used and sensitivity analysis was conducted using a multivariable logistic regression model with cluster robust standard errors. The prevalence of HIV among FSWs in the six regional headquarter towns was estimated to be 11.8% (95% CI: 7.9–17.1); MSM was 3.4% [95% CI: 1.9–5.8]; TGs was 4.2% (95% CI: 2.9–6.1); PWIDs was 4.2% (95% CI: 2.7–6.4) and PI was 3.7% (95% CI: 1.4–9.6). The correlates of HIV test positivity among KPs and PIs include HIV-related knowledge, marital status, district, income, age and sex of KP, level of education, alcohol intake, injecting drugs, and use of lubricants. HIV prevalence is relatively high among FSWs, MSMs, PWID, and TGs as compared to the previous estimate of the general population. There is a need to scale up and strengthen evidence-based HIV prevention interventions such Pre-Exposure Prophylaxis and needle and syringe exchange programmes targeting KPs, including prison inmates. Government must scale up both non-clinical and clinical routine HIV and STI testing and counseling services at the correctional center and drop-in centers for KPs screening/testing, and ensure that services are responsive to the needs of KP.

Ants Along the Street: Street Vending as a Lifeblood of the Urban Poor in Dilla Town, Southern Ethiopia

This article investigates the perspectives and experiences of street vendors in Dilla Town, Southern Ethiopia, focusing on their motivations for engaging in business and social mobility. The study used a descriptive survey design and gathered data through a questionnaire survey, in-depth interviews, and non-participant observations. A time-location sampling technique was applied to select 250 vendors, and an additional 15 were interviewed using a convenience sampling procedure. The data were analyzed using a combination of quantitative and qualitative data analysis techniques. The results revealed that vendors chose street vending due to unemployment, the aspiration for unbridled autonomy, low entry barriers, minimal initial investment requirements, social connections, and the necessity to bolster meager salaries. Most street vendors reported a better standard of living since embarking on their vending venture. The study recommends that local government authorities should acknowledge the socio-economic significance of street vending business for economically disadvantaged urban populations.

Implementation of a sample design for a survey of program participants using time-location sampling.

To assess the feeding practices and behaviors of women and young children participating in the Special Supplemental Nutrition Program for Women, Infants, and Children (WIC), USDA currently funds the longitudinal WIC Infant and Toddler Feeding Practices Study-2 (WIC ITFPS-2). In 2013, the study used time-location sampling (TLS) to enroll a cohort of infants who participated in WIC around birth. The children are subsequently followed across their first six years of life, regardless of their participation in WIC, with an additional follow-up at age nine years. A woman may enroll her child in WIC either during pregnancy or postpartum. For this study, a representative sample of infants enrolled in WIC was desired. Because the associations between WIC prenatal support and education and feeding practices and behaviors are substantively important to this study, the sample needed to include both women enrolling their children prenatally and women enrolling their children postnatally. For prenatal WIC enrollees, we attempted to complete a prenatal interview with the mother prior to the child's birth. This paper describes the TLS approach used and the challenges addressed in implementation of the sample design and selection for the WIC ITFPS-2. Our approach generated a probability sample (subject to site geographic and size exclusions) using a stratified, multistage design, but there were challenges at each stage of selection. First, a WIC site was selected, and then newly enrolled WIC participants were sampled within selected sites during predetermined recruitment windows based on the site's average flow of new WIC enrollees. We discuss issues faced, including overcoming incomplete lists of individual WIC sites and discrepancies between projected new WIC enrollment counts and actual flow of new WIC enrollments during the recruitment period.

Prevalence of Domestic Violence against Women and Its Visibility in Southeast Iran.

We aimed to estimate the prevalence of domestic violence against women at Reproductive Age (WARA) and its visibility in southeast of Iran. Adopting a modified time-location sampling, we recruited 933 WARA in the city of Kerman, Iran from Aug to Dec 2019. Domestic violence (DV) was divided into three main categories: Physical, psychological, and sexual. Data were obtained by direct and Network Scale-Up (NSU) methods through self-administered questionnaires. Generalized Estimating Equations (GEE) was used to determine the association between socioeconomic variables and the violence experience. Visibility was defined as the ratio of NSU over direct estimates. Using the direct method, the annual prevalence of psychological violence was estimated at 60.9%. Corresponding figures for physical and sexual violence were 34.7% and 37.7%, respectively. NSU estimates were about one-third of the direct estimates. Divorced and widowed, self-employed, and less educated women were more likely to experience DV. While the average DV was as high as 44%, its visibility was as low as 33%. Nearly two-thirds of domestic violence against women remains undisclosed. This indicates a high level of stigma perceived around this type of violence.

Sociodemographic characteristics, riding behavior and motorcycle crash involvement: a structural equation modeling approach.

The increasing rate of traffic crashes involving motorcyclists have turned into a public health and road safety concern. Furthermore, riding behaviors and their precedent factors have been identified as potential determinants for assessing, intervening, and preventing traffic injuries of motorists. This study aimed to identify the effects of a set of demographic and motorcycle-related variables as potential predictors on collision through riding behavior components. The study sample was 1,611 motorcyclists who were selected through time-location sampling method from three cities in Iran. They responded a Motorcycle Rider Behavior Questionnaire (MRBQ) and a general questionnaire including sociodemographic and riding-related items. The chosen method to analyze the data was Structural Equation Modeling (SEM) through Lavaan package version 0.6-8 of R software version 4.1.0. All participants were male (100%) with a mean age of 28.1(SD=8.5) years. About 24.4% of riders experienced at least one crash during the last year and the majority of riders did not hold a motorcycle license (80.1%). The SEM model showed that riding license (0.06) and frequency of riding (0.09) had a direct effect on crash involvement. Some latent variables including speed violation (0.13), stunts (0.11) and traffic violation (0.07) had positive effects and safety violation (-0.07) had a negative effect on crash history. There were indirect effects between age and history of crash mediated by speed violation (-0.04), stunts (-0.04), traffic violation (-0.02) and safety violation (0.01). Also, the indirect effects of riding frequency on crash involvement were mediated by speed violation (0.01), traffic violation (0.006) and safety violation (-0.01). This study's main finding is that age and riding frequency are the main variables indirectly affecting crash involvement. Therefore, periodic training courses for younger riders is essential in order to decreasing crash involvements.

Factors Associated with Driving Behavior and Road Traffic Accidents among Public Vehicle Drivers in Kathmandu Valley: A Cross-sectional Study

Introduction: Vehicle drivers are most significant stakeholder in any road traffic accident (RTAs). This study aims to assess their knowledge related to traffic signs and attitude towards safe driving practices and to identify self-reported risky driving behaviors, their encounter of RTAs, and associated factors.&#x0D; Methods: We carried out a cross-sectional study in Kathmandu valley. 14 prime spots were randomly selected and time location sampling was done. A face-to-face interview was done with public vehicle drivers using structured questionnaire. We analyzed the association using logistic regression. Ethical clearance was obtained from Institute of Medicine. Informed written consent was taken from the participants.&#x0D; Results: Of 411 drivers, mean age was 31.8 (±8.2) years. All participants were male of which 71.0% lived in a joint family. More than half had less than 10 year experience and 20% of them were driving 12-18 hours per day. Half of them had good knowledge on traffic signs while nearly one tenth had non-supportive attitude towards safe driving practices. Prevalence of at least one risky driving behavior was 68%, however, only a few cases fined by traffic. Self-reported encounters of RTA were 21.7%; of these, 22.2% also had human injuries. Experiences of driving less than 10 years and living in a nuclear family were significantly associated with risky driving. Moreover, age of public vehicle drivers less than 25 years, living in nuclear family and continuing driving while fatigue were also significantly associated with RTAs.&#x0D; Conclusion: Only half of drivers had good knowledge on traffic signs. Risky driving behavior present in about seven among ten public vehicle drivers and two among ten encountering RTAs. We recommend interventions targeted to change behavior among drivers to reduce risky driving behaviors, to increase age bar for permitting driving license for public vehicles and not continuing driving while fatigue to reduce RTAs.

Prevalence of hepatitis B and C, HIV, and syphilis among people who inject drugs in Germany

Abstract Background We piloted a future monitoring system among people who inject drugs (PWID) in Germany (DRUCK2.0). Monitoring is needed to inform and support the viral hepatitis/HIV/STI elimination process in Germany by providing regular up-to-date prevalence and behavioural data for this key population. Methods PWID aged 16+ years who injected drugs within the last 12 months were recruited by time location sampling via low threshold drug services and opioid substitution treatment (OST) practices during routine services in Berlin and Bavaria between 01/06/2021 and 28/02/2022. All participants filled a questionnaire on sociodemographics, behaviour and access to care and were tested for hepatitis B and C (HBV, HCV), HIV and syphilis using capillary dried blood spots. All received a 10 Euro incentive voucher. Results In total, 495 PWID were included, median age was 39 years [range 18-66], 68% (336/494) were male, and 23% (114/492) born outside Germany, mostly in eastern Europe. Of all participants, 58% (275/477) reported recent use of unsafe needles/syringes and 77% (372/485) detention experience. Current OST was reported by 62% (304/487). Prevalence was 46% (229/495) for cured HCV, 26% (130/495) for active HCV, 17% (80/482) for cured HBV, 1.2% (6/483) for active HBV, 2.7% (13/482) for HIV and 2.1% (10/473) for previous Syphilis. Serological HBV vaccination coverage was 24% (115/475). Of all, 95% (453/475) reported previous HCV testing. Of those with cured/active HCV infection 88% (296/337) knew about their infection and 56% (161/285) of them reported previous/current treatment. Conclusions To decrease the heavy burden of infection among PWID in Germany, targeted measures regarding access to HCV treatment, HBV vaccination, and harm reduction (safer use measures, OST) need to be implemented and rolled out. Nationwide regular monitoring of indicators in this key population is needed to guide the elimination progress of viral hepatitis and HIV in Germany. Key messages • High burden of active Hepatitis C infection and low Hepatitis B vaccination coverage among people who inject drugs in Berlin and Bavaria require improved access to treatment and prevention efforts. • National Monitoring is needed to inform and support the viral hepatitis/HIV/STI elimination process among people who inject drugs in Germany.

Molecular and spatial epidemiology of HCV among people who inject drugs in Boston, Massachusetts.

Integration of genetic, social network, and spatial data has the potential to improve understanding of transmission dynamics in established HCV epidemics. Sequence data were analyzed from 63 viremic people who inject drugs recruited in the Boston area through chain referral or time-location sampling. HCV subtype 1a was most prevalent (57.1%), followed by subtype 3a (33.9%). The phylogenetic distances between sequences were no shorter comparing individuals within versus across networks, nor by location or time of first injection. Social and spatial networks, while interesting, may be too ephemeral to inform transmission dynamics when the date and location of infection are indeterminate.

Acceptability of multiple modalities of pre-exposure prophylaxis (PrEP) among female sex workers in Tanzania: a mixed-methods study

ObjectivesModalities of pre-exposure prophylaxis (PrEP) for HIV prevention offer options to women at high risk including female sex workers (FSW). This study aimed to explore FSW’s acceptability and preferences for...